• Proceedings of the Korea Society of Poultry Science Conference
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• 2005.11a
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• pp.78-79
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• 2005

Effects of dietary lactose or killed Salmonella on the performance, immune response and anti-oxidant system was studied in chicks innoculated with Salmonella typhimurium. In 27 days of age broiler, dietary lactose decreased performance, while dietary lactose and killed Salmonella elevated plasma peroxidase activity and IL-1 level in supernatant of PBMC stimulated with LPS. When broiler chicks innoculated with Salmonella, performance, activities of erythrocyte MnSOD and plasma peroxidase were enhanced after 7 days of the innoculation. Dietary lactose and killed Salmonella increased activity of erythrocyte MnSOD, plasma peroxidase, proliferation of PBMC stimulated with LPS and IL-1 level in the supernatant after 15 days of the innoculation. The result indicated that dietary lactose and killed Salmonella have modulated innate immune response and antioxidant system in broiler chicks innoculated with Salmonella typhimurium.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.52 no.3
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• pp.289-295
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• 2007

This study was conducted to investigate the potential of polyphenols and polyphenol oxidase activity on the induction of rusty symptom development in ginseng root. When rusty inducing bacteria were inoculated on fresh ginseng root, the hue value of the inoculated root increased from 101.2 (white yellow) at 1 day after innoculation to 60.9 (brownish red) at 30 days after innoculation. Lysobacter gummosus, Pseudomonas veronii and Agrobacterium tumefaciens enhanced the accumulation of total phenolics. Along with the increase of total phenolics, total activity of polyphenol oxidase concomitantly increased but the specific activity of the enzyme was not.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2002.09a
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• pp.122-124
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• 2002

The removal capacity of zero-valent iron for Cr(Ⅵ) was evaluated using batch kinetic tests. The rate constants for zero-valent iron dramatically increased as initial Cr(Ⅵ) concentration decreased. Generally, the reaction rates of Cr(Ⅵ) with zero-valent iron were faster than that of a biotic degradation of Cr(Ⅵ), and furthermore the reaction rates were inversely proportional to the initial Cr(Ⅵ) concentrations. After certain reaction time elapsed. no further decrease of Cr(Ⅵ) was observed, indicating a loss of iron reactivity. The loss of iron reactivity was primarily due to the passivation of iron surfaces with iron-Cr precipitates, but the reactivity of iron was recovered by adding iron-reducing bacteria. Even though the addition of bacteria itself removed Cr(Ⅵ), the combination of iron-reducing bactera and oxidized iron significantly enhanced the reaction rate for Cr(Ⅵ) removal. The results from column tests also confirmed that the innoculation of iron-reducing bacteria to the column containing completely oxidized iron partially enhanced the recovery of the iron reactivity.

• Journal of Plant Biotechnology
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• v.42 no.4
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• pp.350-355
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• 2015

Clubroot disease is one of the most wide-spread and devastating diseases in the cultivation of Chinese cabbage. To develop a protein marker for resistance to clubroot disease in Chinese cabbage, a comparative proteome analysis was performed between a sensitive line, 94SK, and a resistant line, CR Shinki DH. Three proteins of two fold or higher accumulation that are specific to each line were found 3 days after innoculation of the Plasmodiphora brassicae. They are glutamine synthetase, malate dehydrogenase/oxidoreductase and fructose-bisphosphate aldolase in the 94SK and actin, phosphoglycerate kinase, and Cu/Zn superoxide dismutase in the CR Shinki line. From the comparison of the synthesized proteins in the 94SK and the CR Shinki, CR Shinki was found to produce more ATP-binding protein for the ABC transporter while 94SK showed a higher level of pathogenesis-related protein 1 production. All of these proteomic variations may lead to the development of molecular markers to accelerate the breeding process.

• Korean Journal of Poultry Science
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• v.27 no.3
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• pp.227-233
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• 2000

Preset study was carried out to evaluate characteristics of lactic acid producing bacteria(LAB) in hen's cecum as probiotics value. Distribution of LAB in intestinal tracts was investigated using 5∼25 weeks - old hens. So, 12 strains to LAB with different morphology were isolated purely. Acid tolerance of LAB tested at pH 1, 2, 3, and 4, and bile resistant also tested at 0, 0.3% and 0.5% bile salt concentration. Growth pattern of LAB observed to 60h. All strains of cecal LAB couldn't survive at pH 1, and decreased linearly survival colony after incubation at pH 2 although some strains could survive for 2h. Most of LAB maintained constant number at pH 3 and 4. The bacterial action could increase linearly at 0% bile salt concentration in all of tested strains. However, only one strain could multiply at 0.3% bile salt, others were influenced by bile salt. That tendency was similar at 0.5% bile salt. Growth was peaked at 12 to 18 h after innoculation. After peak, the decreasing pattern of colony was different to strains which some strains decreased rapidly or maintained for long time. The LAB of hen's cecum was similar to intolerance acidity, but different to resistant to bile salt and growth pattern by strain. So, we choose three strains which have probiocs value, and identified as Lactobacillus amylovorus LLA7, Lactobacillus crispatus LLA9 and Lactobacillus vaginalis LLA11.

• Korean Journal of Environment and Ecology
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• v.29 no.1
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• pp.88-94
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• 2015

An investigation was conducted on the effects of arbuscular mycorrhizal fungus, Glomus etunicatum on the formation of soil aggregate and mycorhizal development in the roots of Robinia pseudoacacia seedlings in coal mine tailings and forest soil. G. etunicatum formed mycorrhizas by 35.1 % in coal mine tailings and by 48.9 % in forest soil. Its infection was the typical Arum-type forming inter-cellular hyphae and intra-cellular arbuscules. Ergosterol contents were 3.20 ppm in forest soil and 1.92 ppm in coal mine tailings. The formation of soil aggregate per 50 g pot soil was 19.6 g and 9.5 g in inoculated and noninoculated forest soil and 16.5 g and 11.0 g in inoculated and non-inoculated coal min tailings, respectively. In conclusion, G. etunicatum inoculation increased the formation of soil aggregate both in forest soil and coal mine tailings, but was less effective in the latter.

• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.681-684
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• 2001

Pseudomonas sp. F721 isolated from soil produced a substance related in seeds germination inhibition. Addition of phytohormone, and GA (gibberellin acid) in the culture broth elevated production of the germination inhibition substance. The production of the substance was optimized in the culture conditions of $35^{\circ}C$, pH 9.0, 150 rpm, 48 hr, glucose 0.5% (w/v), and innoculation ratio 1.0% (v/v). The physical and chemical stability of the substance in the variety of pH ranging from 2.0 to 12.0 and from freezing to $100^{\circ}C$ were shown. The germination inhibition substance suppressed 90% of germination compared with that of the control experiment in a few days.

• KSBB Journal
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• v.16 no.6
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• pp.620-625
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• 2001

This study was examined to investigate the time course behaviors of cell growth and sucrose consumption, and effects of various elicitors on ginsenoside production in batch suspension cultures of Panax ginseng Meyer. Suspended cells reached to the stationary phase at 12 days after innoculation. The maximum cell concentration was 14.7 g-DCW/L at 17 days. The highest cell growth rate was 0.59 g-DCW/L d. The sucrose used as a sole carbon source was hydrolysed to glucose and fructose in 4 days, then quickly utilized until the middle-log phase and consumpted completely at 16 days. Various elicitors were app1ied at 8 days from inoculation which is the middle-log phase. Among the elicitors tested, jasmonic acid was the most efficient to increase the ginseneside production, which was 1.5 times higher than control.

• Biomedical Science Letters
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• v.2 no.2
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• pp.257-265
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• 1996

Simple stain and electron microscopic in situ hybridization is studied and applied for the identification of rabbit haemorrhagic disease viral RNA in a unicrylated preparation of the liver after innoculation of rabbit haemorrhagic disease virus. Hybridization for detection of viral RNA in unicryl embedded tissues using complementary 84 bases oligonucleotide probe labelled by biotin CE-phosphoramidite compared with 4717∼4800 sequences of rabbit haemorrhagic disease virus, modified hybridization protocol and antibiotin antibody-l0nm gold as signal marker. The best results were obtained in 0.02% glutaraldehyde, Unicryl resin cell block, biotinylated oligonucleotide probes, antibiotin-l0nm gold. In this report, RHD viral RNA was distributed widely within the mitochondria and nucleus of liver cell by electron microscopic in situ hybridization. In situ hybridization has become a standard method for localizing DNA or RNA sequences in tissue or celt preparation. In situ hybridization is detected the virus genome in the cells and tissue as specifically compared with others nucleic acid hybridization method.

• Korean journal of applied entomology
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• v.45 no.3 s.144
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• pp.339-346
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• 2006

Total 90 species of medicinal plants were surveyed to see if they have any suppressive effects on the dinsity of M hapla at the exhibition field in the Chinan medicinal herbs experiment station. In 70 species including Achyranthes japonica, root-knot and/or egg sac of M. hapla was not found and these plants were planted in C. lanceolata field to check the degree of M. hapla infection. In 26 species including A. japonica, M. hapla infection was not observed. Simultaneously, 30 species were planted in pots to find out degree of infection by M. hapla. Dianthus chinensis, Rudbeckia bicolor, Sedum kantschaticum, Ricinus communis, Anemarrhena asphodeloides, Malva verticillate, Chelidonium majus, Sesamum indicum, Agrimonia pilosa, Geum aleppicum, Sanguisorba officinalis and Scrophularia buergeriana were free from infection. While the number of galls and density of M. hapla in soil were higher to high innoculation density, and the growth of C. lanceolata was rower.