Browse > Article
http://dx.doi.org/10.5010/JPB.2015.42.4.350

Root proteome analysis of Chinese cabbage in response to Plasmodipohora brassicae Woron  

Jeung, Jae Yun (Department of Crop Science and Biotechnology, Dankook University)
Lim, Yong Pyo (Department of Horticulture, Chungnam National University)
Hwang, Cheol Ho (Department of Crop Science and Biotechnology, Dankook University)
Publication Information
Journal of Plant Biotechnology / v.42, no.4, 2015 , pp. 350-355 More about this Journal
Abstract
Clubroot disease is one of the most wide-spread and devastating diseases in the cultivation of Chinese cabbage. To develop a protein marker for resistance to clubroot disease in Chinese cabbage, a comparative proteome analysis was performed between a sensitive line, 94SK, and a resistant line, CR Shinki DH. Three proteins of two fold or higher accumulation that are specific to each line were found 3 days after innoculation of the Plasmodiphora brassicae. They are glutamine synthetase, malate dehydrogenase/oxidoreductase and fructose-bisphosphate aldolase in the 94SK and actin, phosphoglycerate kinase, and Cu/Zn superoxide dismutase in the CR Shinki line. From the comparison of the synthesized proteins in the 94SK and the CR Shinki, CR Shinki was found to produce more ATP-binding protein for the ABC transporter while 94SK showed a higher level of pathogenesis-related protein 1 production. All of these proteomic variations may lead to the development of molecular markers to accelerate the breeding process.
Keywords
Clubroot disease; Plasmodiphora brassicae; Proteome; Root;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Jang CS, Piao Z, Park YJ, Lim YP (2001) Development of anther-derived lines resistant to clubroot disease (Plasmodiophora brassicae Woron.) in Chinese cabbage. J Kor Hort Sci 42:689-694
2 Kaido M, Ishikawa T, Hori H (2007) Comparative proteomic analysis of the resistant response in root culture to the clubroot disease agent Plasmodiophora brassicae. Bull Facul Agric Niigata Univ 60:67-71
3 Kim DW, Oh JH (1977) Incidence, pathogenicity of clubroot fungus (Plasmodiophora brassicae) and varietal resistance in Chinese cabbage. Kor J Plant Pathol 13:95-99
4 Kobayashi Y, Yamada M, Kobayashi I, Kunoh H (1997) Actin microfilaments are required for the expression of nonhost resistance in higher plants. Plant Cell Physiol 38:725-733   DOI
5 Krattinger SG, Lagudah ES, Spielmeyer W, Singh RP, Huerta-Espino J, McFaddenn H, Bossolini E, Selter LL, Keller B (2009) A putative ABC transporter confers durable resistance to multiple fungal pathogens in wheat. Science 323:1360-1363   DOI
6 Lebel E, Heifetz P, Thorne L, Uknes S, Ryals J, Ward (1998) Functional analysis of regulatory sequences controlling PR-1 gene expression in Arabidopsis. The Plant J 16:223-233   DOI
7 Neuhoff VN, Arold N, Taube D, Ehrhardt W (1988) Improved staining of protein in polyacrylamide gel including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250. Electrophoresis 9:255-262   DOI
8 Ouibrahim L, Mazier M, Estevan J, Pagny G, Decroocq V, Desbiez C, Moretti A, Gallois J-L, Caranta C (2014) Cloning of the Arabisopsis rwm1 gene for resistance to Watermelon mosaic virus points to a new function for natural virus resistance genes. The Plant J 79:705-716   DOI
9 Pagano EA. Chueca A. Lopez-Gorge J (2000) Expression of thioredoxins f and m, and of their targets fructose-1,6- bisphosphatase and NADP-malate dehydrogenase, in pea plants grown under normal and light/temperature stress conditions. J. Exp. Bot. 51:1299-1307   DOI
10 Piao ZY, Park YJ, Choi SR, Hong CP, Park JY, Choi YS, Lim YP (2002) Conversion of AFLP marker linked to clubroot resistance gene in chinese cabbage into a SCAR marker. J. Kor. Soc. Hort. Sci. 43(6):653-659
11 Piao ZY, Deng YQ, Cho SR, Park YJ, Lim YP (2004) SCAR and CAPS mapping of CRb, a gene conferring resistance to plasmodiophora brassicae in Chinese cabbage (Brassica rapa ssp. pekinensis) Theor Appl Genet 108:1458-1465   DOI
12 Rabilloud T (1992) A comparison between low background silver diammine and silver nitrate protein stains. Electrophoresis 13:429-439   DOI
13 Seifi HS, Bockhaven JV, Angenon G, Hofte M (2013) Glutamate metabolism in plant disease and defense: Friend or Foe? MPMI 26:75-485   DOI
14 Subramanian B, Bansal VK, Kav NNV (2005) Proteome-level investigation of Brassica carinata-derived resistance to Leptosphaeria maculans. Agr and Food Chem 53:313-324   DOI
15 Sun C, Wang L, Hu D, Riquicho AR, Liu T, Hou X, Li Y (2014) Proteomic analysis of non-heading Chinese cabbage infected with Hyaloperonospora parasitica. J Proteomics 98:15-30   DOI
16 Williams PH (1996) A system for the determination of race of Plasmodiophora brassicae that infect cabbage and rutabaga. Phytopathology 56:624-626
17 Yu CY, Hu SW, Zhao HX, Guo AG, Sun GL (2005) Genetic distances revealed by morphological characters, isozymes, proteins and RAPD markers performance in oilseed rape (Brassica napus L.). Theor. Appl. Genet. 110:511-518   DOI