• 한방안이비인후피부과학회지
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• 제17권1호
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• pp.45-54
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• 2004

Major symptoms of allergic rhinitis are nasal obstructions, sneezing and watery rhinorrhea. Gamigyeji-tang has been used to treat for watery rhinorrhea, which is one of the symptoms of allergic rhinitis. This experimental study was done to rescarch effects of Gamigyeji-tang. We have studied effect of mice on OVA-induced Production of IL-4, IL-5, IFN-${\gamma}$ by Murine Splenocytes, and effect of OVA-induced total IgE and OVA-Specific IgE. The results were as follows ; 1. In IL-4 study, Gamigyeji-tang treated group was proved significant inhibitory effect(p〈0.005) 2. In IL-5 study, Gamigyeji-tang treated group was proved significant inhibitory effect.(p〈0.05) 3. In IFN-${\gamma}$ study, Gamigyeji-tang treated group was proved significant inhibitory effect(p〈0.000001) 4. In Total IgE, Gamigyeji-tang treated group didn't showed significant inhibitory effect. 5. In OVA-specific IgE, Gamigyeji-tang treated group didn't showed significant inhibitory effect. According to this result, Gamigyejj-tang was concluded to be effective on anti-allergic action. More studies are required to investigate the mechanism of inhibition by herbal medicine in allergic rhinitis model.

• Restorative Dentistry and Endodontics
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• 제20권1호
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• pp.275-288
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• 1995

The inhibitory effect of cell wall extracts of streptococci, have been investigated to know host-parasite relationship or pathogenesis of abscess formation. Streptococci isolated from the infected root canals were sonicated to get cell wall extracts which have been known as one of the factors of pyogenesis. Lymphocytes separated by density gradient were stimulated with phytohemagglutinin and exposed to cell wall extracts of Streptococcus sanguis, S. mitis, S. uberis, S. mutans (ATCC 10449), and S. faecalis (ATCC 19433). [$^3H$]-thymidine uptake of lymphocytes was analyzed with scintillation counter and lactate dehyrogenase (LD) activity was measured with autochemistry analyzer. S. faeealis had the strongest inhibitory effect. beginning at $100\;{\mu}g/ml$ concentration of sonic extracts. S. sanguis and S. mitis had inhibitory effect at $300\;{\mu}g/ml$, while S. uberis and S. mutans showed no inhibitory, effect on DNA syntheis even at $300\;{\mu}g/ml$. Each streptococci showed different inhibitory effect on the DNA synthesis of lymphocytes, which finding indicated wide spectrum of susceptibility of lymphocytes according to streptococcus spp. There were no significant difference of LD activities between control and each streptococcal extracts. Streptococcal sonic extracts did not affect the morphological findings or number of colonies activated lymphocytes. These finding suggested the inhibitory effect of sonic extract of streptococci to lymphocytes could be detected by DNA synthesis inhibition, not by cellular membrane damage.

• KSBB Journal
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• 제8권1호
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• pp.75-82
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• 1993

The inhibitory effect of cacao bean husk (CBH) extract on glucosyltransferase(GTasc) from Streptococcus mutans B13 was investigated. Water solube extract from CBH showeda sarong inhibitory effect (88-89%) on GTase from Streptococcus mutans Bl3. GTase inhibitors from sequential extraction by hot water or water-methanol had the strongest inhibition. Sources, fermentation, and types of solvents and fumigation processes did not influence the effect. These active compounds proved to be polyphones through acid hydrolytic analysis of the precipitates by ammonium sulfate or ethanol and proteinase K. It was also confirmed by additional column chromatography of Sephadex G-50, Sephadex LH-20 and DEAE-Sephdex A-50.

• 한국약용작물학회지
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• 제28권3호
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• pp.195-199
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• 2020

Background: The inhibitory effect of Rodgersia podophylla root extract on melanin production has been reported, however, the study on the optimal extraction conditions that increase melanin inhibitory activity has not yet been performed. Methods and Results: In this study, we compared the melanin inhibitory activity of R. podophylla root extract obtained through different preprocessing and extraction methods. The melanin inhibitory activity was examined using Melan-A melanocytes. The results indicated that the inhibitory activity of R. podophylla roots collected in August was higher than that of the roots collected in May and November. Additionally, non-dried R. podophylla roots exhibited higher activity than dried roots, and the stirring extract was more active than the ultrasonic extract. Conclusions: Collection of R. podophylla root when the temperature is high, without drying, and stirring extraction are considered to be the optimal extraction conditions for increased melanin inhibitory effect.

• 한국식품영양학회지
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• 제6권2호
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• pp.109-114
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• 1993

This study was divised to observe an Inhibitory effect toward a lipolytic action of toxohormone-L from large root and small root Nepal pseudo ginseng (NPG ; Nepal products) components by water extract and ethanol precipitate in vitro. Toxohormone-L is known to be a lipolytic factor that was partially purified from the ascites fluid of Sarcoma 180-bearing mice and of patients with hepatoma. The inhibitory effect that inhibited the lipolytic action of toxohormone-L by ethanol Precipitate component of large root NPG (mean 55.5%) was higher (mean 1.37 times) than that of water extract component in final reaction concentration of 500 and 1, 000ug/ml, on the other side inhibitory effect of water extract component in small root NPG (mean 55.5%) was higer (mean 1.14 times) than that of ethanol precipitate component. In a way inhibitory effect of precipitate component In large root NPG(47.6%), when final reaction concentration of sample were 1, 000ug/ml, was about 40% lower than that of Korean red ginseng.

• 대한수의학회지
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• 제21권1호
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• pp.1-6
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• 1981

In order to observe the bactericidal effect of Ranunculus species on pathogenic bacteria, the minimal inhibitory concentration and bactericidal effect was tested Throughout the studies, the following experimental results were obtained and summarized. 1. Minimal inhibitory concentration of Ranunculus species extracts on E. coli was observed in the medium in which 1% Ranunculus species extracts added to brain heart infusion agar. 2. Minimal inhibitory concentration of Ranunculus species extracts on Salmonella species observed in the medium in which 1% Ranunculus species extracts added to brain heart infusion agar. 3. Minimal inhibitory concentration of Ranunculus species extracts on Staphylococcus and Streptococcus was observed in the medium in which 1.5% Ranunculus species extracts added to brain hrart infusion blood agar. 4. The Bactericidal effect of Ranunculus species extracts on E. coli and S. typhi was observed in 30 minutes. 5. The Bactericidal effect of Ranunculus species extracts on staphylococcus aureus was obserded in 40 minutes.

• 동의생리병리학회지
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• 제19권3호
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• pp.710-715
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• 2005

This research was to investigate inhibitory activity of Sasang medicines on Obesity. 56 kind of herb medicines to powder, abstract add 100 times methanol. Examine inhibitory effect against lipase activity in vitro. It was compared Xenical(Orlistat) with 56 kind of herb medicines. Inhibitory effect against lipase activity of Xenical as a standard was 93.3%. It is Inhibitory effect against lipase activity of Taeyangin herb medicine. (Fructus Pruni Tomentosae 77.7% Cortex Acanthopanacis73.5%, Fructus Chaenomelis73.1%, rice bran on a mallet head68.1%, Semen Fagopyri 48.1%, Radix Vitis 31.5%) It is Inhibitory effect against lipase activity of Soyangin herb medicine. (Radix Saposhnikoviae 91.5%, Semen Plantaginis 90.4%, Semen Trichosanthis 89.2%, Herba Schizonepetae 85.7%, Radix Euphorbiae Kansui 76.1%, Rhizoma Anemarrhenae 76.0%, Cortex Phellodendri 75.1%, Herba Menthae 74.8%, Radix Angelicae Pubescentis 61.2%, Rhizoma Alismatis 62.6%, Poria 60.9%, Rhizoma Notopterygii 22.5%, Radix Peucedani 18.2%, Caulis Akebiae 17.7%) It is Inhibitory effect against lipase activity of Taeeumin herb medicine. (Herba Spirodelae 91.4%, Radix Polygalae 88.3%, Arillus Longanae 84.3%, Radix Platycodi 81.7%, Semen Zizyphi Spinosae 80.2%, Spina Gleditsiae 79.7%, Herba Ephedrae 75.3%, Semen Raphani 73.1%, Rhizoma Cimicifugae 73.0%, Rhizoma Acori Graminei 71.8%, Flos Chrysanthemi71.0%, Radix et Rhizoma Rhei 68.4%, Vermiculus Holotrichia 59.9%, Radix Puerariae 55.3%, Fructus Schizandrae 53.4% Semen Coicis 50.6%, Semen Biotae 47.5%, Semen Nelumbinis 46.7%, Radix Angelicae Dahuricae 45.1%, Semen Castaneae 44.4%, Rhizoma Ligustici 34.5%, Tuber Liriopis 16.4%, Radix Scutellariae4.2%) It is Inhibitory effect against lipase activity of Soeumin herb medicine. (Pericarpium Arecae89.9%, Rhizoma Alpiniae Officinarum 89.5%, Radix Polygoni Multiflori81.2%, Rhizoma Cyperi 79.0%, Cortex Magnoliae 72.8%, Radix Aucklandiae 72.0%, Rhizoma Zingiberis 71.9%, Pericarpium Citri Reticulatae Viride 71.3%, Radix Cynanchi Wilfordi 57.7% Rhizoma Pinelliae 36.7%, Fructus Alpiniae Oxyphyllae 35.8% Fructus Aurantii Immaturus 17.5%, Pericarpium Citri 16.1%) Therefore, Radix Saposhnikoviae(91.5) and Herba Spirodelae(91.4%) were the most effective medicice of 56 kind of medicines.

• Journal of Microbiology and Biotechnology
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• 제12권5호
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• pp.834-837
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• 2002

A fluorescent glucose analogue,2-[N-(7-nitrobenz-2-ox a-1,3-diazol-4-yl) amino] -2- deoxy-D-glucose (2-NBDG), which had previously been developed for the analysis of glucose uptake in living cells, was investigated to determine its biological activity on microorganisms.2-NBDG did not show any inhibitory effect on growth of yeast cells and bacteria. In contrast, 2-NBDG exhibited strong inhibitory effects on filamentous fungal growth. The growth of filamentous fungi was completely inhibited, when 2-NBDG was supplemented as sole carbon source. The inhibitory effect was decreased by the addition of glucose in the test medium. Furthermore, 2-NBDC inhibited chitinase activity of Trichoderma sp. These results suggested that the inhibitory effects of 2-NBDG on filamentous fungi might be partially due to the inhibition of chitinase.

• The Korean Journal of Physiology and Pharmacology
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• 제1권6호
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• pp.797-808
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• 1997

Nitric oxide (NO) has been 3mown as a mediator of nonadrenergic, noncholinergic inhibitory neurotransmitter in intestinal smooth muscles. It has been suggested that NO donor such as sodium nitroprusside (SNP) produces relaxation of smooth muscle via activation of guanylate cyclase and elevation of cGMP levels. We have therefore investigated the effects of NO, using SNP, on muscle tension in the longitudinal smooth muscle of guinea-pig ileum. The possible role of cGMP was also investigated as well as the involvement of $K^+$ channel on SNP-induced inhibitory effect. The results are summarized as follows; high KCI-or CCh-activated contractions were inhibited by SNP in a concentration-dependent manner. 8-Br-cGMP also showed a similar effect in that of SNP TEA (1 mM) significantly reduced the SNP-induced inhibitory effect. SNP-induced effect was forther reduced by the presence of 10 mM TEA. On the other hand, 4-AP (0.1 mM), glibenclamide $(10\;{\mu}M)$ and apinain $(0.1\;{\mu}M)$ showed little effects on SNP-induced relaxation. Zaprinast significantly potentiated the SNP-induced inhibitory effect in all ranges. ODQ also significantly decreased the SNP-induced inhibitory effect. Pretreatment with CPA $(10\;{\mu}M)$ slightly reduced the SNP-induced inhibitory effect. From the above results, both effect mediated by NO and cGMP might be responsible for the activation of $Ca^{2+}$-activated $K^+$ channel by SNP in guinea-rig ileum. And this $K^+$ channel activation by SNP also contributes to the SNP-induced membrane hyperpolarization and relaxation.

• 한방안이비인후피부과학회지
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• 제17권1호
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• pp.55-65
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• 2004

Allergic rhinitis is an inflammation of the nasal mucosa which is triggered by an allergic reaction. When exposed to certain allergens, the IgE covered mast cells degranulate releasing inflammatory mediators and cytokines which results in a local inflammatory reaction. In many recent studies, molecular biological methods have been used to investigate the role of cytokines in pathogenesis and new therapeutic targets of allergic rhinitis. This experimental study was done to research effects of Sochungryong-tang. We have studied effect of mice on OVA-induced production of IL-4, IL-5, IFN-${\gamma}$ by murine splenocytes and effect of OVA-induced total IgE and OVA-specific IgE. The results were as follows; 1. In IL-4 study, Sochungryong-tang treated group was proved significant inhibitory effect(p〈0.005). 2. In IL-5 study, Sochungryong-tang treated group was proved significant inhibitory effect(p〈0.05). 3. In JFN-${\gamma}$ study, Sochungryong-tang treated group was proved significant inhibitory effect(p〈0.000001). 4. In Total IgE, Sochungryong-tang treated group was proved significant inhibitory effect(p〈0.000001). 5. In OVA-specific IgE, Sochungryong-tang treated group didn't showed significant inhibitory effect. Depending upon above results, it is considered that Sochungryong-tang has the inhibitory effect on the allergic rhinitis of mice and suggested that it could be used in relieving patients of the symptoms which are caused by allergic rhinitis.