THE INHIBITORY EFFECT OF STREPTOCOCCAL CELL WALL EXTRACTS ON STIMULATION OF LYMPHOCYTES

연쇄구균의 세포벽 단백질 추출물이 림프구 활성의 억제에 미치는 영향

  • Sang, Hyoung-Sook (Department of Conservative Dentistry, College of Dentistry, Wonkwang University) ;
  • Jeong, Hee-Il (Department of Conservative Dentistry, College of Dentistry, Wonkwang University) ;
  • Oh, Se-Hong (Department of Conservative Dentistry, College of Dentistry, Wonkwang University) ;
  • Im, Mi-Kyung (Department of Conservative Dentistry, College of Dentistry, Wonkwang University)
  • 상현숙 (원광대학교 치과대학 치과보존학교실) ;
  • 정희일 (원광대학교 치과대학 치과보존학교실) ;
  • 오세홍 (원광대학교 치과대학 치과보존학교실) ;
  • 임미경 (원광대학교 치과대학 치과보존학교실)
  • Published : 1995.03.10

Abstract

The inhibitory effect of cell wall extracts of streptococci, have been investigated to know host-parasite relationship or pathogenesis of abscess formation. Streptococci isolated from the infected root canals were sonicated to get cell wall extracts which have been known as one of the factors of pyogenesis. Lymphocytes separated by density gradient were stimulated with phytohemagglutinin and exposed to cell wall extracts of Streptococcus sanguis, S. mitis, S. uberis, S. mutans (ATCC 10449), and S. faecalis (ATCC 19433). [$^3H$]-thymidine uptake of lymphocytes was analyzed with scintillation counter and lactate dehyrogenase (LD) activity was measured with autochemistry analyzer. S. faeealis had the strongest inhibitory effect. beginning at $100\;{\mu}g/ml$ concentration of sonic extracts. S. sanguis and S. mitis had inhibitory effect at $300\;{\mu}g/ml$, while S. uberis and S. mutans showed no inhibitory, effect on DNA syntheis even at $300\;{\mu}g/ml$. Each streptococci showed different inhibitory effect on the DNA synthesis of lymphocytes, which finding indicated wide spectrum of susceptibility of lymphocytes according to streptococcus spp. There were no significant difference of LD activities between control and each streptococcal extracts. Streptococcal sonic extracts did not affect the morphological findings or number of colonies activated lymphocytes. These finding suggested the inhibitory effect of sonic extract of streptococci to lymphocytes could be detected by DNA synthesis inhibition, not by cellular membrane damage.

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