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PCR-RFLP and Sequence Analysis of the rDNA ITS Region in the Fusarium spp.

  • Min, Byung-Re;Lee, Young-Mi;Choi, Yong-Keel
    • Journal of Microbiology
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    • v.38 no.2
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    • pp.66-73
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    • 2000
  • To investigate the genetic relationship among 12 species belonging to the Fusarium section Martiella, Dlaminia, Gibbosum, Arthrosporiella, Liseola and Elegans, the internal transcribed spacer(ITS) regions of ribosomal DNA (rDNA) were amplified with primer pITS1 and pITS4 using the polymerase chain reaction(PCR). After the amplified products were digested with 7 restriction enzymes, restriction fragment length polymorphism (RFLP) patterns were analyzed. The partial nucleotide sequences of the ITS region were determined and compared. Little variation was observed in the size of the amplified product having sizes of 550bp or 570bp. Based on the RFLP analysis, the 12 species studied were divided into 5 RFLP types. In particular, strains belonging to the section Martiella were separated into three RFLP types. Interestingly, the RFLP type of F. solani f. sp. piperis was identical with that of isolates belonging to the section Elegans. In the dendrogram derived from RFLP analysis of the ITS region, the Fusarium spp. examined were divided into two major groups. In general, section Martiella excluding F. solani f. sp. piperis showed relatively low similarity with the other section. The dendrogram based on the sequencing analysis of the ITS2 region also gave the same results as that of the RFLP analysis. As expected, 5.8S, a coding region, was highly conserved, whereas the ITS2 region was more variable and informative. The difference in the ITS2 region between the length of F. solani and its formae speciales excluding F. solani f. sp. piperis and that of other species was caused by the insertion/deletion of nucleotides in positions 143-148 and 179-192.

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Prenatal diagnosis of an unbalanced translocation between chromosome Y and chromosome 15 in a female fetus

  • Lee, Dongsook;Park, Heeju;Kwak, Sanha;Lee, Soomin;Go, Sanghee;Park, Sohyun;Jo, Sukyung;Kim, Kichul;Lee, Seunggwan;Hwang, Doyeong
    • Journal of Genetic Medicine
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    • v.13 no.2
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    • pp.95-98
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    • 2016
  • We report the prenatal diagnosis of an unbalanced translocation between chromosome Y and chromosome 15 in a female fetus. Cytogenetic analysis of parental chromosomes revealed that the mother had a normal 46,XX karyotype, whereas the father exhibited a 46,XY,der(15)t(Y;15) karyotype. We performed cytogenetic analysis of the father's family as a result of the father and confirmed the same karyotype in his mother and brother. Fluorescence in situ hybridization and quantitative fluorescent-polymerase chain reaction analysis identified the breakpoint and demonstrated the absence of the SRY gene in female members. Thus, the proband inherited this translocation from the father and grandmother. This makes the prediction of the fetal phenotype possible through assessing the grandmother. Therefore, we suggest that conventional cytogenetic and molecular cytogenetic methods, in combination with family history, provide informative results for prenatal diagnosis and prenatal genetic counseling.

A Study on Barrier-free Certification Evaluation of the Bus Terminals (여객자동차터미널의 장애물 없는 생활환경 인증 평가에 관한 연구)

  • Kang, Byoung-Keun;Kang, Tae-Sung;Kim, Sang-Woon;Lee, Joo-Hyung
    • Journal of The Korea Institute of Healthcare Architecture
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    • v.22 no.2
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    • pp.7-14
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    • 2016
  • Purpose: Caring for weak person in society, which increases the population of the elderly and people with disabilities is essential. By increasing along with the increase of the use of passenger facilities for travelers and pedestrian is increasing the demand for convenient and secure facilities for everyone. The emerging importance of the passenger-related facilities, but a representative study of the bus terminal facilities is lacking. Bus terminals are the most popular passenger facility. Thus, the bus terminal can be conveniently used by anyone in everyday life, however, the applied elements considering the use of the transportation Poor are not sufficient. Methods: This study was conducted to evaluate BF certification standards targeting bus terminals across the country to determine the availability of the transportation Poor in bus terminal. Result: As a result of the bus Terminal possibility BF certified it appeared to be very low. Also, items received the lowest rating of each item was evaluated in the informative facilities and items. Bus terminal is a facility used by the unspecified individuals, the proportion of first-time user is high, but there is a lack of consideration for the transportation Poor bus terminals. Implications: In the future, this study can guide the next research on the application of BF certification standards in bus terminal. Further studies can be presented to the improvement of the BF certification indicator in bus terminal.

Secured Telemedicine Using Whole Image as Watermark with Tamper Localization and Recovery Capabilities

  • Badshah, Gran;Liew, Siau-Chuin;Zain, Jasni Mohamad;Ali, Mushtaq
    • Journal of Information Processing Systems
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    • v.11 no.4
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    • pp.601-615
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    • 2015
  • Region of interest (ROI) is the most informative part of a medical image and mostly has been used as a major part of watermark. Various shapes ROIs selection have been reported in region-based watermarking techniques. In region-based watermarking schemes an image region of non-interest (RONI) is the second important part of the image and is used mostly for watermark encapsulation. In online healthcare systems the ROI wrong selection by missing some important portions of the image to be part of ROI can create problem at the destination. This paper discusses the complete medical image availability in original at destination using the whole image as a watermark for authentication, tamper localization and lossless recovery (WITALLOR). The WITALLOR watermarking scheme ensures the complete image security without of ROI selection at the source point as compared to the other region-based watermarking techniques. The complete image is compressed using the Lempel-Ziv-Welch (LZW) lossless compression technique to get the watermark in reduced number of bits. Bits reduction occurs to a number that can be completely encapsulated into image. The watermark is randomly encapsulated at the least significant bits (LSBs) of the image without caring of the ROI and RONI to keep the image perceptual degradation negligible. After communication, the watermark is retrieved, decompressed and used for authentication of the whole image, tamper detection, localization and lossless recovery. WITALLOR scheme is capable of any number of tampers detection and recovery at any part of the image. The complete authentic image gives the opportunity to conduct an image based analysis of medical problem without restriction to a fixed ROI.

3D Graphic Nursery Contents Developed by Mobile AR Technology (모바일 기반 증강현실 기술을 활용한 3D전래동화 콘텐츠 연구)

  • Park, Young-sook;Park, Dea-woo
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.20 no.11
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    • pp.2125-2130
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    • 2016
  • In this paper, we researched the excellency of 3D graphic nursery contents which is developed by mobile AR technology. AR technology has currently people's attention because of the potential to be core contents of future ICT industry. We applied AR nursery contents for kid's subtitle language selection in Korean, Chinese and English education. The original fairy tale consisted of 6~8 scenes for the 3D contents production, and was adapted and translated. Dubbing was dubbed by the native speaker using the standard pronunciation, and the effect sound was edited separately to fit the scene. After composing a scenario, constructing a 3D model, constructing a interaction, constructing a sound effect, and creating content metadata, the Unity 3D game engine is executed to create a project and describe it as a script. It provides a fun and informative tradition of fairy tales with abundant content that incorporates ICT technology, accepting advanced technology-based education, and having opportunities to perceive software in daily life.

Social Media based Real-time Event Detection by using Deep Learning Methods

  • Nguyen, Van Quan;Yang, Hyung-Jeong;Kim, Young-chul;Kim, Soo-hyung;Kim, Kyungbaek
    • Smart Media Journal
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    • v.6 no.3
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    • pp.41-48
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    • 2017
  • Event detection using social media has been widespread since social network services have been an active communication channel for connecting with others, diffusing news message. Especially, the real-time characteristic of social media has created the opportunity for supporting for real-time applications/systems. Social network such as Twitter is the potential data source to explore useful information by mining messages posted by the user community. This paper proposed a novel system for temporal event detection by analyzing social data. As a result, this information can be used by first responders, decision makers, or news agents to gain insight of the situation. The proposed approach takes advantages of deep learning methods that play core techniques on the main tasks including informative data identifying from a noisy environment and temporal event detection. The former is the responsibility of Convolutional Neural Network model trained from labeled Twitter data. The latter is for event detection supported by Recurrent Neural Network module. We demonstrated our approach and experimental results on the case study of earthquake situations. Our system is more adaptive than other systems used traditional methods since deep learning enables to extract the features of data without spending lots of time constructing feature by hand. This benefit makes our approach adaptive to extend to a new context of practice. Moreover, the proposed system promised to respond to acceptable delay within several minutes that will helpful mean for supporting news channel agents or belief plan in case of disaster events.

Analysis of the Phylogenetic Relationships in the Genus Spiraea Based on the Nuclear Ribosomal DNA ITS Region (핵 리보솜 DNA ITS 부위에 의한 조팝나무속 식물종의 계통 관계 분석)

  • Huh, Man-Kyu
    • Journal of Life Science
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    • v.22 no.3
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    • pp.285-292
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    • 2012
  • Genus Spiraea is composed of many long-lived woody species that are primarily distributed throughout Asia and Europe. In this study, we evaluated a representative sample of the 38 taxa in the world, including 14 in Korea, with nuclear ribosomal DNA internal transcribed spacer sequences (ITS) to estimate genetic relationships within the genus. The molecular data allowed us to resolve well-supported clades in the taxa. In 47 world accessions (38 taxa: 14 Korean taxa, 33 world taxa, and 9 overlapping taxa), total alignment length was 689 positions, of which 452 were parsimony informative, 527 variable, 75 singleton, and 159 constant characters. Although the phylogenic tree showed that many taxa of genus Spiraea were well separated from each other, many branches were not congruent with the morphological characteristics and geographical distributions of the genus. There were 430 segregating sites and the nucleotide diversity (${\pi}$) value was 0.281. Under the neutral mutation hypothesis, the probability that the Tajima test statistic (D) is positive (2.325) is more than 0.5. Therefore, there may be a site at which natural selection, which increases genetic variation, is operating.

Histopathologic Changes to Additive Effect of Endocrine Disruptors in Mice (마우스에서의 환경호르몬물질의 상가작용에 대한 조직학적인 변화)

  • Jung, Ji-Youn
    • Journal of Food Hygiene and Safety
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    • v.21 no.4
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    • pp.258-262
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    • 2006
  • To investigate additive effects of endocrine disruptors, we have histopathologically studied the exchanges of the reproductive organ to ovariectomized ICR mice. Female ICR mice were ovariectomized and then treated with endocrine disruptors after two weeks. Macroscopic exchanges, which were body weight, feed and water intake, of all groups were not seen during experiment period. Histopathological changes of uterine epithelial cells, vaginal epithelial cells, mammary glands and the diameter in uterine tubles were observed. In the results, the histopathological sensitivity to endocrine disruptors effect was more seen to the vaginal epithelial cell height than others. The additive estrogenic effects of endocrine disruptors, which were combinations of DEHP, DBP and BPA, were seen with E2 and BPA treatments. These results offers a sysmatic and mechanistically informative approach to assessing estrogenicity. It provides a useful profile of activity using a reasonable amount of resources and is compatible with the study of individual chemicals as well as the investigation of interactions among combinations of chemicals.

The Implement of System on Microarry Classification Using Combination of Signigicant Gene Selection Method (정보력 있는 유전자 선택 방법 조합을 이용한 마이크로어레이 분류 시스템 구현)

  • Park, Su-Young;Jung, Chai-Yeoung
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.12 no.2
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    • pp.315-320
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    • 2008
  • Nowadays, a lot of related data obtained from these research could be given a new present meaning to accomplish the original purpose of the whole research as a human genome project. In such a thread, construction of gene expression analysis system and a basis rank analysis system is being watched newly. Recently, being identified fact that particular sub-class of tumor be related with particular chromosome, microarray started to be used in diagnosis field by doing cancer classification and predication based on gene expression information. In this thesis, we used cDNA microarrays of 3840 genes obtained from neuronal differentiation experiment of cortical stem cells on white mouse with cancer, created system that can extract informative gene list through normalization separately and proposed combination method for selecting more significant genes. And possibility of proposed system and method is verified through experiment. That result is that PC-ED combination represent 98.74% accurate and 0.04% MSE, which show that it improve classification performance than case to experiment after generating gene list using single similarity scale.

Role of Features in Plasma Information Based Virtual Metrology (PI-VM) for SiO2 Etching Depth (플라즈마 정보인자를 활용한 SiO2 식각 깊이 가상 계측 모델의 특성 인자 역할 분석)

  • Jang, Yun Chang;Park, Seol Hye;Jeong, Sang Min;Ryu, Sang Won;Kim, Gon Ho
    • Journal of the Semiconductor & Display Technology
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    • v.18 no.4
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    • pp.30-34
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    • 2019
  • We analyzed how the features in plasma information based virtual metrology (PI-VM) for SiO2 etching depth with variation of 5% contribute to the prediction accuracy, which is previously developed by Jang. As a single feature, the explanatory power to the process results is in the order of plasma information about electron energy distribution function (PIEEDF), equipment, and optical emission spectroscopy (OES) features. In the procedure of stepwise variable selection (SVS), OES features are selected after PIEEDF. Informative vector for developed PI-VM also shows relatively high correlation between OES features and etching depth. This is because the reaction rate of each chemical species that governs the etching depth can be sensitively monitored when OES features are used with PIEEDF. Securing PIEEDF is important for the development of virtual metrology (VM) for prediction of process results. The role of PIEEDF as an independent feature and the ability to monitor variation of plasma thermal state can make other features in the procedure of SVS more sensitive to the process results. It is expected that fault detection and classification (FDC) can be effectively developed by using the PI-VM.