• Journal of Microbiology and Biotechnology
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• v.28 no.11
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• pp.1916-1927
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• 2018

Corynebacterium glutamicum is an excellent platform for the production of amino acids, and is widely used in the fermentation industry. Most industrial strains are traditionally obtained by repeated processes of random mutation and selection, but the genotype of these strains is often unclear owing to the absence of genomic information. As such, it is difficult to improve the growth and amino acid production of these strains via metabolic engineering. In this study, we generated a complete genome map of an industrial L-valine-producing strain, C. glutamicum XV. In order to establish the relationship between genotypes and physiological characteristics, a comparative genomic analysis was performed to explore the core genome, structural variations, and gene mutations referring to an industrial L-leucine-producing strain, C. glutamicum CP, and the widely used C. glutamicum ATCC 13032. The results indicate that a 36,349 bp repeat sequence in the CP genome contained an additional copy each of lrp and brnFE genes, which benefited the export of L-leucine. However, in XV, the kgd and panB genes were disrupted by nucleotide insertion, which increase the availability of precursors to synthesize L-valine. Moreover, the specific amino acid substitutions in key enzymes increased their activities. Additionally, a novel strategy is proposed to remodel central carbon metabolism and reduce pyruvate consumption without having a negative impact on cell growth by introducing the CP-derived mutant $H^+$/citrate symporter. These results further our understanding regarding the metabolic networks in these strains and help to elucidate the influence of different genotypes on these processes.

• The Korean Journal of Pharmacology
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• v.24 no.1
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• pp.1-10
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• 1988

To obtain information about the structure of the human phenylethanolamin N-methyltransferase (PNMT) and to further define the extent of the evolutionary relationships among PNMT molecules of several spesies, a full length cDNA clone for bovine adrenal PNMT was used to screen a charon 4A genomic library. One phage was isolated and identified, which included the entire PNMT gene. The length of inserted genomic DNA was 13.1-Kilobase (Kb) containing two internal EcoRI sites. Construction of a restriction map and subsequent Southern and dot blot analysis with 5'-and3'-specific cDNA probes allowed the identification of exon-containing fragments. This is the first report of the cloning of gene for human epinephrine synthesizing enzyme.

• The Journal of the Korea Contents Association
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• v.9 no.11
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• pp.465-474
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• 2009

To measure the amount of nonpoint source pollution, some efforts are tried to utilize satellite imagery. But, as the factors for water models do not relate with the landcover categories for satellite imagery, satellite imagery are adapted to roughly classified thematic map or used only for the image interpretation. The purpose of this study is to establish the landcover categories of satellite imagery to relate with the water models. To establish the categories of the landcover for the water models, it was investigated to get main factors of water flow models for the nonpoint source pollution and to review the existing study and the classification system. For this result, it was convinced that the basic unit on the nonpoint source pollution, landcover coefficients of SCS Curve Number, the crop factor of Universal Soil Loss Equation, Manning's roughness coefficients are the useful parameters to extract information from the satellite imagery. After the setup the categories for the landcover classification, it was finally defined from the consultation of the water model specialist. Woopo wetland watershed was selected to the study area because it is a representative wetland in Korea and needs the management system for nonpoint source pollution. There were used Landsat ETM Plus and SPOT-5 satellite imagery to assess the result of the image classification.

• Journal of Korean Society for Atmospheric Environment
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• v.26 no.1
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• pp.10-20
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• 2010

In recent studies, $PM_{2.5}$ has been reported to be more harmful to human health than $PM_{10}$ because it penetrates more deeply into the lung. We estimated $PM_{2.5}$ related health benefits in Seoul from implementing the World Health Organization (WHO)'s guidelines (annual average $10{\mu}g/m^3$, 24-hour average $25{\mu}g/m^3$) and U.S. Environmental Protection Agency (EPA)'s National Ambient Air Quality Standard (annual average $15{\mu}g/m^3$, 24-hour average $35{\mu}g/m^3$). U.S. EPA's Environmental Benefits Mapping and Analysis Program was utilized for the analysis. It was predicted that the attainment of the WHO annual guideline and U.S. EPA's annual standard, relative to the concentration in 2006, would result in reduction of 2,333~2,895 premature deaths and 1,703~2,121 premature deaths, respectively. If the WHO and EPA's daily standard for $PM_{2.5}$ are attained, 1,211~1,394 and 1,012~1,165 premature deaths could be avoided, respectively. Sensitivity analyses indicated that the estimates were robust regardless of air quality simulation methods for attaining the $PM_{2.5}$ goals. This study provides a quantitative approach to evaluate health risks from air pollution as well as to assess the potential health benefits of improving atmospheric $PM_{2.5}$ concentration. Even considering the intrinsic limitations and uncertainties of the analysis, it is an important information to rationalize the enforcement of $PM_{2.5}$ management policies and measures in Seoul, Korea.

• Journal of Navigation and Port Research
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• v.35 no.1
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• pp.57-62
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• 2011

On the view point of renewable energies as energy sources of district heating and cooling plant, the purpose of this study is to develop, classify and map the 500m${\times}$500m mesh, of which is treated as normal size in DHC regulations for evaluation process. Followings are the results. Various building and geographical informations including 13 districts and 108 counties are re-defined to create 500m${\times}$500m meshes, and it is find out that 3,289 meshes among 8,463 meshes have meaningful floor areas. Only 59 meshes(1.8%) are evaluated as mesh which has more than 50% of building volume ratio per mesh. 5 clusters classified by principal analysis and cluster analysis with building needs' characteristics are defined. Gwang-an Dong is representative of cluster 1 characterized as commercial area, and the cluster 4, 5 which has mainly residential needs are distributed in Yong-ho dong. Because there are a lot of cluster 3 meshes, which has complex needs area based on residential, cluster 3 could be defined as representative of Busan metropolitan city.

• Journal of KIISE:Software and Applications
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• v.36 no.12
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• pp.967-976
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• 2009

In this paper, we propose an automatic lower extremity vessel extraction based on rigid registration and bone elimination techniques in CT and CT angiography images. First, automatic partitioning of the lower extremity based on the anatomy is proposed to consider the local movement of the bone. Second, rigid registration based on distance map is performed to estimate the movement of the bone between CT and CT angiography images. Third, bone elimination and vessel masking techniques are proposed to remove bones in CT angiography image and to prevent the vessel near to bone from eroding. Fourth, post-processing based on vessel tracking is proposed to reduce the effect of misalignment and noises like a cartilage. For the evaluation of our method, we performed the visual inspection, accuracy measures and processing time. For visual inspection, the results of applying general subtraction, registered subtraction and proposed method are compared using volume rendering and maximum intensity projection. For accuracy evaluation, intensity distributions of CT angiography image, subtraction based method and proposed method are analyzed. Experimental result shows that bones are accurately eliminated and vessels are robustly extracted without the loss of other structure. The total processing time of thirteen patient datasets was 40 seconds on average.

• Journal of Cadastre & Land InformatiX
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• v.47 no.1
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• pp.127-142
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• 2017

The purpose of this study is to examine the status of urbanization of Namyangju-Si from 2001 till 2008, analyze the correlation of the change of surrounding land use according to the change of accessibility and analyze the correlation of the change of land use. Based on the result from correlation analysis, I simulated from the view point of interactions between agents of land use and transportation accessibility by using MAS. I tried to forecast, based on space, the change of surrounding land use caused by the change of accessibility. I conducted landscape analysis by using a land registration map. Also, I conducted GIS analysis statistical analysis simulation analysis of traffic data, land use data in order to analyze the correlation of the change of surrounding land use according to the change of accessibility. I could find out a certain pattern that new roads and railroads causes the within 500m adjacent land to change into urban land use, which led me to construct a system through which land use changing phenomenon can be expected according to the change of accessibility. It is expected that it can make the best use of selecting the location where new transportation facilities are constructed.

• Proceedings of the Korean Association of Geographic Inforamtion Studies Conference
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• 2006.05a
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• pp.211-216
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• 2006

교통주제도는 건설교통부와 한국교통연구원이 ${\ulcorner}$국가교통DB구축사업${\lrcorner}$의 일환으로 제작한 것으로, 국토지리정보원의 NGIS 수치지도(축척 1:5,000)를 기반으로 하여 현장조사 및 문헌조사를 통해 갱신, 보완하여 교통 분야의 활용에 적합하도록 구축한 GIS 수치지도이다. 교통주제도는 현장조사와 문헌조사를 통해 수집된 자료를 기반으로 구축되며 이중 교통시설물조사는 문헌자료(준공도로현황 등)를 참고하여 산정된 조사대상에 대하여 GPS 조사시스템을 활용, 도형 및 속성정보를 취득하는 것을 말한다. 조사항목은 도형정보(노드, 링크) 및 속성정보(차선수, 도로번호 등)이며 GPS 조사시스템과 조사원장(도면)을 이용하여 조사한다. 교통시설물조사와 문헌자료조사를 통해 수집된 자료는 단위도엽(1:5,000기준)별로 교통주제도에 반영되며 최종적으로 전국단위의 통합자료로 구축된다 교통주제도는 사용목적과 축척기준에 따라 단순화되며 이를 교통주제도 레벨이라 한다. 교통주제도의 레벨은 총 4단계로 구분되며 현재 레벨2에서 레벨4까지 구축되고 있다. 교통분석용 네트워크는 교통시설투자계획, 수요분석, 타당성 평가와 같은 교통분야의 연구를 수행하기 위해 반드시 필요한 자료이다. 교통분석용 네트워크는 교통주제도보다 더 단순화된 도로망 모델로서 구성요소는 노드(시점, 종점, 죤 센트로이드)와 링크(도로망, 센트로이드 커넥터)이며 도로의 기하구조보다는 논리적 구조(방향성, 노드/링크 연결성 등)에 중점을 둔 데이터이다 교통분석용 네트워크는 교통주제도의 도형과 속성정보를 기반으로 하며 각종 통계자료(도로교통량통계연보 등)를 활용, 구축된다. 교통분석용 네트워크는 구축시점에 따라 현재 및 장래 네트워크로 구분되며 장래 네트워크는 기준년도부터 2031년까지 5년 단위로 계획된 장래도로를 반영하여 구축된다. 교통주제도 및 교통분석용 네트워크는 국가교통DB구축사업을 통해 구축된 자료로서 교통체계효율화법 제9조의4에 따라 공공기관이 교통정책 및 계획수립 등에 활용할 수 있도록 제공하고 있다. 건설교통부의 승인절차를 거쳐 제공하며 활용 후에는 갱신자료 및 활용결과를 통보하는 과정을 거치도록 되어있다. 교통주제도는 국가의 교통정책결정과 관련분야의 기초자료로서 다양하게 활용되고 있으며, 특히 ITS 노드/링크 기본지도로 활용되는 등 교통 분야의 중요한 지리정보로서 구축되고 있다.

• IMMUNE NETWORK
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• v.5 no.4
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• pp.237-246
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• 2005

Background: Little information is available on the identification and characterization of the upstream regulators of the signal transduction cascades for Mycobacterium tuberculosis (M. tbc)-induced ERK 1/2 activation and chemokine expression. We investigated the signaling mechanisms involved in expression of CCL3 /MIP-1 and CCL4/MIP-1 in human primary monocytes infected with M. tbc. Methods: MAP kinase phosphorylation was determined using western blot analysis with specific primary antibodies (ERK 1/2, and phospho-ERK1/2), and the upstream signaling pathways were further investigated using specific inhibitors. Results: An avirulent strain, M. tbc H37Ra, induced greater and more sustained ERK 1/2 phosphorylation, and higher CCL3 and CCL4 production, than did M. tbc H37Rv. Specific inhibitors for mitogen-activated protein kinase (MAPK) kinase (MEK; U0126 and PD98059) significantly inhibited the expression of CCL3 and CCL4 in human monocytes. Mycobactetia-mediated expression of CCL3 and CCL4 was not inhibited by the Ras inhibitor manumycin A or the Raf-1 inhibitor GW 5074. On the other hand, phospholipase C (PLC) inhibitor (U73122) and protein kinase C (PKC)specific inhibitors ($G\ddot{o}6976$ and Ro31-8220) significantly reduced M. tbc-induced activation of ERK 1/2 and chemokine synthesis. Conclusion: These results are the first to demonstrate that the PLC-PKC-MEK-ERK, not the Ras-Raf-MEK-ERK, pathway is the major signaling pathway inducing M. tbc-mediated CCL3 and CCL4 expression in human primary monocytes.

• Journal of The Korean Astronomical Society
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• v.51 no.3
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• pp.49-63
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• 2018

We carry out a study of Sub-Millimeter Galaxies (SMGs) in the AKARI NEP-Deep field using the James Clerk Maxwell Telescope (JCMT) SCUBA-2 $850{\mu}m$ source catalog, released as part of the SCUBA-2 Cosmology Legacy Survey (S2CLS) program. The SCUBA-2 $850{\mu}m$ map has a root mean square (rms) noise of $1.2mJy\;beam^{-1}$ and covers an area of $0.60degree^2$. We find four SMGs which have counterparts to Herschel sources with spectroscopic redshifts in the literature. In addition, three dust obscured galaxies (DOGs) detected in Herschel bands are selected as a comparison sample. We derive IR luminosities of SMGs using the CIGALE code, which are similar to those of high redshift SMGs from previous studies. The contribution of AGN to the total IR luminosity in SMGs (2%-11%) is smaller than the lower limit for the one in DOGs (19%-35%), which is consistent with the expectation from the evolutionary scenario of massive galaxies. We search for SMGs in overdense regions as protocluster candidates and investigate four regions, including candidates around three DOGs. Finally, we argue that follow-up spectroscopic observation for the NEP-Deep field will provide crucial information to understand the role of SMGs in the evolution of massive galaxies.