• Journal of Periodontal and Implant Science
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• 제30권4호
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• pp.747-757
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• 2000

Cyclosporin A(CsA) is an immunosuppressive agent widely used for preventing graft rejecting response in organ transplantation. The basic properties of CsA to osteoblast has not been well known yet. A better understanding of the mechanisms of CsA function on bone could provide valuable information regarding basic properties of bone remodeling, pharmacotherapeutic intervention in metabolic bone disease, and the consequences of immunosuppression in bone physiology. The purpose of this study was to investigate the effect of CsA on osteoblast by evaluating parameters of proliferation, collagen synthetic activity, alkaline phosphatase activity, and ALP mRNA expression in mouse calvarial cell. 1. CsA ($3{\mu}g/m{\ell}$) treated mouse calvarial cell showed statistically significant increase in cell proliferation.(P<0.05) 2. CsA($1,\; 3{\mu}g/m{\ell}$) treated MC3T3 cell line showed statistically significant increase in cell proliferation. 3. The amount of collagen of CsA($3{\mu}g/m{\ell}$) treated mouse calvarial cell was decreased statistically significantly. 4. Alkaline phosphatase activity was increased statistically significantly in CsA treated group($1{\mu}g/m{\ell}$). 5. mRNA expression of ALP was increased in CsA treated group These results suggest that CsA could affect bone remodeling by modulating proliferation & differentiation of osteoblast.

• 복식문화연구
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• 제10권2호
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• pp.200-212
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• 2002

The purpose of this study is to make the internet website on the order-made fashion design so as to do meet the need of netizen in the 21st century. It made the consumer do the design which he or she wants by the combination of the compositions that do select the pattern of the skirt design to fit his or her size and body shape. For the set-up of website, the elements of composition must be considered, and also the planning and design must be done fur the order-made fashion design. With the internet website, it's possible to do reach the below points; first, it can make the consumer do the design which he or she wants by himself or herself; second, it can provide him or her the information of measuring his or her body shape to buy the design pattern which he or she wants. Third, it can be contributed to make the brand more active by improving his or her satisfaction with the design and pattern which he or she wants. Also it can bring up the effects as fellows; first, lashing power of the design can be increased by the order-made fashion design to attract his or her interest, and also it can make him or her more satisfied. Second, the competitiveness can be strengthened by the differentiation of the fashion site between the existing design and the order-made fashion design. Third, the production of design can be higher and also its expense can be reduced even for the small and medium-sized clothes maker or merchants. Finally, it is able to enlarge the applicable field into the design of small articles, home fashion, and textile which they want to sell the differentiated and order-made products.

• 융합정보논문지
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• 제10권3호
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• pp.58-66
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• 2020

이 연구는 인터넷전문은행의 국내 금융소비자의 신뢰 수준을 토대로 연령대별 신뢰도 차이에 대해 다단 비교를 통해 실증 분석하고자 하였다. 이로써 국내 금융시장에 인터넷전문은행이 착근할 수 있는 토대에 대한 기초 연구 자료를 구축하고, 연령대별로 소비자 신뢰 구축 전략을 어떻게 차별화할 것인지에 대한 단초를 제공하려는 것이 이 연구의 목적이다. 이를 위해 먼저 이 연구에서는 연령대별로 금융소비자 그룹을 2030세대 그룹과 4050세대 그룹으로 분리하여 이 두 그룹의 신뢰감을 결정하는 요인을 탐색하고, 두 그룹의 차이를 다집단 분석방법을 통해 비교하였다. 연구 결과, 전 연령층에서 인터넷전문은행에 대한 금융소비자 신뢰는 편리성, 보안성 그리고 금융 거래 비용의 합리성이 결정요인으로 작용함을 보았다. 그리고 연령대별로 과연 신뢰 결정요인이 영향력에서 차이가 있는지를 살펴본 결과, 3개의 신뢰 결정요인 중 비용의 합리성 요인이 젊은 층과 중장년층 간 신뢰 수준의 차이를 발생시키는 것으로 확인되었다. 그리고 소비자 신뢰는 추후 관계몰입으로 역시 강하게 연결됨도 재확인되었다. 이러한 연구 결과는 앞으로 시장 경쟁이 격화될 인터넷전문은행이 지속성장하기 위해 소비자 신뢰가 무엇보다 중요함을 시사한다고 하겠다.

• 지적과 국토정보
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• 제50권1호
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• pp.25-45
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• 2020

본 연구의 주된 목적은 녹지의 공간 분포 패턴을 식별하는데 있어 국지적 공간자기상관 기법들의 성능을 비교하고 분석하는 것이다. 이 연구목적을 달성하기 위해 본 연구는 위성영상분석기법과 공간자기상관기법들을 이용하였다. 분석의 결과 공간 특이치 군집을 갖는 LISA 군집지도가 도시녹지의 공간 분포 패턴을 식별하는 데 있어서 다른 분석기법들보다 우수함이 확인되었다. 본 연구는 기존의 연구들과는 다른 몇 가지 연구방법을 이용했다는 점에서 관련분야에 기여할 수 있다. 이러한 차별성과 유용성에도 불구하고 본 연구는 녹지의 공간적 분포패턴을 식별하는 있어서 저해상도 위성영상을 이용했다는 점과 식생지수들 중에서 NDVI만을 이용했다는 점에서 한계를 지닌다. 이러한 한계들은 향후연구에서 UAV영상을 이용하거나 또는 여러 가지 식생지수들을 동시에 이용한다면 극복될 수 있을 것이다.

• Molecules and Cells
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• 제39권4호
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• pp.316-321
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• 2016

The receptor activator of nuclear factor ${\kappa}B$ (RANK) and its ligand RANKL are key regulators of osteoclastogenesis and well-recognized targets in developing treatments for bone disorders associated with excessive bone resorption, such as osteoporosis. Our previous work on the structure of the RANK-RANKL complex revealed that Loop3 of RANK, specifically the non-canonical disulfide bond at the tip, performs a crucial role in specific recognition of RANKL. It also demonstrated that peptide mimics of Loop3 were capable of interfering with the function of RANKL in osteoclastogenesis. Here, we reported the structure-based design of a smaller peptide with enhanced inhibitory efficiency. The kinetic analysis and osteoclast differentiation assay showed that in addition to the sharp turn induced by the disulfide bond, two consecutive arginine residues were also important for binding to RANKL and inhibiting osteoclastogenesis. Docking and molecular dynamics simulations proposed the binding mode of the peptide to the RANKL trimer, showing that the arginine residues provide electrostatic interactions with RANKL and contribute to stabilizing the complex. These findings provided useful information for the rational design of therapeutics for bone diseases associated with RANK/RANKL function.

• Journal of Gastric Cancer
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• 제10권3호
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• pp.91-98
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• 2010

Purpose: Silent mating-type information regulation 2 homologue 1 (SIRT1) is a nicotinamide adenine dinucleotide-dependent deacetylase. SIRT1 plays an important role in the regulation of cell death/survival and stress response in mammals. The aim of this study was to investigate whether the SIRT1 gene is involved in the development or progression of gastric cancers. Materials and Methods: SIRT1 and p53 genes in 86 gastric cancers were examined for genetic alterations by PCR-single strand conformation polymorphism sequencing, as well as SIRT1 protein expression in 170 gastric cancers by immunohistochemistry. Results: In the genetic analysis, we found SIRT1 and p53 mutations in two and 12 cases, respectively. Two missense mutations, c.599 C>T (T200I) and c.1258 G>A (E420K), were detected in the SIRT1 gene coding region. The SIRT1 and p53 mutation were found in mutually exclusive gastric cancers. The immunohistochemistry revealed that SIRT1 overexpression was found in 95 (55.9%) of 170 gastric cancers. Altered SIRT1 expression was not statistically associated with clinicopathological parameters, including tumor differentiation, location, lymph node metastasis, or p53 expression. Two cases with an SIRT1 mutation showed increased SIRT1 expression. Conclusions: These results suggest that genetic alterations and overexpression of the SIRT1 gene may contribute to gastric cancer development.

• 한국항해항만학회:학술대회논문집
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• 한국항해항만학회 2006년도 International Symposium on GPS/GNSS Vol.1
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• pp.327-332
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• 2006

In airborne gravimetry, there are two data streams. One is the specific force measured by an air/sea gravimeter or accelerometers, the other is kinematic acceleration measured by DGPS. And the difference of them provides the gravity disturbance information. To satisfy the requirement of most applications, an accuracy of 1mGal $(1mCal=10^{-5}m/s^{2})$ with a spatial resolution of 1km is the aim of current airborne gravimetry. There are two different methods to derive the kinematic acceleration. The generally used method is to differentiate the position twice, and the position can be calculated by commercial DGPS software. The main defect of this method is that integer ambiguities need to be fixed to get the precise position solution, but it's not a trivial thing for long base line. And to fix integer ambiguities, the noisier iono-free measurement is used. When differentiation is applied, noise is amplified and will influence the accuracy of acceleration. The other method is to get carrier phase acceleration by differentiate the carrier phase first, and then using the acceleration of GPS satellite to derive the vehicle acceleration. The main advantages include that fixing integer ambiguities is not needed anymore, position can be relaxed to about 10 meters, and smoother acceleration can be got since iono-free measurement is not needed. In some literatures, it's considered that the dynamic performance of the second method is inferior to that of the first. Through analysis, it is found that the performance degradation in dynamic environment results from the simplification of the GPS carrier phase observable model. And an iterative algorithm is presented to compensate the model error. Using a dynamic GPS data from an aeromagnetic survey, the importance of this compensation is showed at last.

• 환경생물
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• 제32권1호
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• pp.1-15
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• 2014

양서류는 육상과 수상생태계를 연결하는 먹이사슬의 연결자로 진화적 생태적 지이를 갖는다. 양서류의 배아와 유생은 모체와 독립되어 수환경 내에서 초기발생 및 성장하기 때문에 수환경에 존재하는 다양한 화학물질에 직접적으로 노출될 수 있다. Azole계열 항곰팡이제는 농업 및 의료용으로 널리 사용되는 화학물질로서 농지, 하수처리장 등으로 부터 수계로 유입된다. 최근, 양서류에서 이러한 azole계 물질에 의한 기형유발, 내분비계장애 효과가 증가하고 있다. 본 소고에서는 azole계 물질의 양서류 독성을 파악하고 azole계 물질의 안전한 이용을 위한 가이드라인을 제공하고자 azole계열에 속하는 imidazole, triazole, thiazole, oxazole, pyrazole 항곰팡이 물질이 양서류의 발생, 분화, 생식 등에 미치는 영향에 대해 최근까지의 연구결과를 정리하였다.

• 동의생리병리학회지
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• 제29권1호
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• pp.39-45
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• 2015

This experiment was designed to investigate the effect of Added Chongmyung-tang (ACM) on Alzheimer's disease mouse model. Effects of ACM on learning behavior were investigated using the Morris water maze method. Expression levels of molecular factors related to Alzheimer's disease such as glial fibrillary acidic protein (GFAP), cluster of differentiation antigen 68 (CD68), and tau protein in the hippocampus of APP-SWE Tg2576 mice were analyzed by immunofluorescence staining method. ACM reduced escape latency in the Morris water maze test. ACM decreased the expression level of GFAP and tau protein in the hippocampus. These results suggest that ACM may be involved in regulating molecules that are known to play an important role in the pathogenesis of Alzheimer's disease.

• 식물조직배양학회지
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• 제25권3호
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• pp.171-175
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• 1998

지황(Rehmannia glutinosa)의 캘러스배양을 통한 기내 대량증식을 위하여 엽조직으로부터 캘러스 형성에 미치는 생장조절물질의 영향을 조사한 결과 MS배지에 0.5 mg/L NAA와 2.0 mg/L BA 첨가배지에서 캘러스 형성율 100%, 식물체 분화율 31%로 가장 높았으며, 2.4-D와 zeatin 첨가배지에서는 캘러스 형성율은 95% 이상 높았으나 기관분화가 전혀 이루어지지 않았다. 재분화 식물체로부터 다수의 신초 유기에 적합한 배지로는 MS배지에 0.2mg/L NAA와 0.5 mg/L BA를 첨가한 배지에서 3.4개와 2.8개로 양호하였다. 다수의 신초 증식을 위하여 1.0 g/L활성탄을 첨가한 배지에서 가장 많은 3.8개의 신초가 분화되었으나 2.0 g/L 첨가배지에서는 2.4개로 감소된 반면, 초장과 엽수는 증가하여 개체생육은 양호함을 알 수 있었다.