Numerous studies have reported that genes with similar expression patterns are co-regulated. From gene expression data, we have assumed that genes having similar expression pattern would share similar transcription factor binding sites (TFBSs). These function as the binding regions for transcription factors (TFs) and thereby regulate gene expression. In this context, various analysis tools have been developed. However, they have shortcomings in the combined analysis of expression patterns and significant TFBSs and in the functional analysis of target genes of significantly overrepresented putative regulators. In this study, we present a web-based A Functional Clustering Analysis Tool for Predicted Transcription Regulatory Elements and Gene Ontology Terms (FCAnalyzer). This system integrates microarray clustering data with similar expression patterns, and TFBS data in each cluster. FCAnalyzer is designed to perform two independent clustering procedures. The first process clusters gene expression profiles using the K-means clustering method, and the second process clusters predicted TFBSs in the upstream region of previously clustered genes using the hierarchical biclustering method for simultaneous grouping of genes and samples. This system offers retrieved information for predicted TFBSs in each cluster using $Match^{TM}$ in the TRANSFAC database. We used gene ontology term analysis for functional annotation of genes in the same cluster. We also provide the user with a combinatorial TFBS analysis of TFBS pairs. The enrichment of TFBS analysis and GO term analysis is statistically by the calculation of P values based on Fisher’s exact test, hypergeometric distribution and Bonferroni correction. FCAnalyzer is a web-based, user-friendly functional clustering analysis system that facilitates the transcriptional regulatory analysis of co-expressed genes. This system presents the analyses of clustered genes, significant TFBSs, significantly enriched TFBS combinations, their target genes and TFBS-TF pairs.
This study aimed to explore the direction and possibility of development of the integrated curriculum for the Gifted students in the discipline-centered curriculum perspective. To this end, the study analysed the Ontario interdisciplinary curriculum based on a Drake's Integration Model which is typical model of the discipline-centered curriculum and explored the applicability to Science Gifted Academy in Korea. Through showing the possibility of enrichment on the selected majors, integration with other disciplines and solving the future problems by the integrated curriculum centered on 'Big Idea', the Ontario interdisciplinary curriculum gave suggestions of curriculum integration within or through individual research and integrated curriculum for the Gifted. The application of the Ontario's "Introduction to Information studies" to "Information Science" in Science Gifted Academy in Korea could be obtained the conclusion that the Drake's Integration model is applied to the Gifted by the individualization of the navigation network, KDB(Knowledge-Do-Be) umbrella, and the final interdisciplinary task. From this result, we could suggest that the development of integrated curriculum for the Gifted should be considered the clarity of learning objectives for the Gifted, the plan of evaluation to demonstrate big understanding and big idea, the integration with other disciplines or real-world problem, as well as the need of teachers council for the integrated curriculum. This study is expected to be contribute to development of the integrated curriculum model for the gifted based on the their characteristics and to be utilized in Science Gifted Academy.
Journal of the Korea Society of Computer and Information
/
v.15
no.12
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pp.197-207
/
2010
Using a variety of data-mining methods on high-throughput cDNA microarray data, the level of gene expression in two different tissues can be compared, and DEG(Differentially Expressed Gene) genes in between normal cell and tumor cell can be detected. Diagnosis can be made with these genes, and also treatment strategy can be determined according to the cancer stages. Existing cancer classification methods using machine learning select the marker genes which are differential expressed in normal and tumor samples, and build a classifier using those marker genes. However, in addition to the differences in gene expression levels, the difference in gene-gene correlations between two conditions could be a good marker in disease diagnosis. In this study, we identify gene pairs with a big correlation difference in two sets of samples, build gene classification modules using these gene pairs. This cancer classification method using gene modules achieves higher accuracy than current methods. The implementing clinical kit can be considered since the number of genes in classification module is small. For future study, Authors plan to identify novel cancer-related genes with functionality analysis on the genes in a classification module through GO(Gene Ontology) enrichment validation, and to extend the classification module into gene regulatory networks.
Purpose - The purpose of this study is to identify team commitment affecting employees' innovative activities and factors affecting team commitment including empowerment by leaders and job enrichment factors. In other words, so as to explain outcome variables of innovative activities, this study aims to emphasize employees' attachment roles towards their groups within nomological network, and identify the motives encouraging employees' innovative activities. The research purpose is significant due to the realistic situation of hotel industry. The reason why innovative activities are important can be found in recent changes of business environment. Also, unlike other various studies on precedence factors encouraging employees' innovative activities, this study classified those precedence factors into job and leader characteristics, and it emphasized the importance of team commitment as the process that job and leader characteristics are connected to innovative activities. Research design, data and methodology - The survey for this study was conducted during October 6th ~ November 10th in 2014 to the employees who are working in 5-star hotels in Korea. As for the selection of hotels and sampling method, convenience sampling method was used to the employees in 5-star hotels. Self-report method was used in the survey, judging that the employees' characteristics would be relatively homogeneous. 311 questionnaires were distributed in total, and 275 reponses were collected. After excluding the missing and unreliable responses, 245 questionnaires were used in the research. SPSS and AMOS programs were used for the analysis. Results - First, empowering leadership had positive effects on hotel employees' team commitment. It indicates that hotel employees are more committed to their team when their leaders set examples, provide information, and involve employees in decision-making process. Second, as a result of the relationship analysis in task diversity, task significance, task identity and team commitment, task diversity and task significance had significant effect on team commitment, while task identity had no significant effect on team commitment. It indicates that team commitment is enhanced when the employees can conduct diverse types of jobs and get more opportunities to talk with the guests. Also, the repetition for the same jobs in hotel rooms and the space for preparing food and beverage do not lead to team commitment, even though the employees fulfill their duties to the end. Third, hotel employees' team commitment has positive effect on their innovative activities. It indicates that employees voluntarily conduct innovative activities when they are attached to their team and identifies themselves with the team. Conclusions - There are theoretical and practical implications in this study. First, in terms of the theoretical perspective, this study proposes structural framework in team commitment, and it identifies the psychological mechanism in team commitment from the aspect of social exchange, which resulted in identification of precedence factors related to team commitment. In addition, this study presents new possibilities for relevant studies about team commitment by examining the effect on team commitment when the importance of innovative activities is emphasized in recent business environment.
Seo, Minseok;Lee, Hyun-Jeong;Kim, Kwondo;Caetano-Anolles, Kelsey;Jeong, Jin Young;Park, Sungkwon;Oh, Young Kyun;Cho, Seoae;Kim, Heebal
Asian-Australasian Journal of Animal Sciences
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v.29
no.3
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pp.343-351
/
2016
Although the chemical, physical, and nutritional properties of bovine milk have been extensively studied, only a few studies have attempted to characterize milk-synthesizing genes using RNA-seq data. RNA-seq data was collected from 21 Holstein samples, along with group information about milk production ability; milk yield; and protein, fat, and solid contents. Meta-analysis was employed in order to generally characterize genes related to milk production. In addition, we attempted to investigate the relationship between milk related traits, parity, and lactation period. We observed that milk fat is highly correlated with lactation period; this result indicates that this effect should be considered in the model in order to accurately detect milk production related genes. By employing our developed model, 271 genes were significantly (false discovery rate [FDR] adjusted p-value<0.1) detected as milk production related differentially expressed genes. Of these genes, five (albumin, nitric oxide synthase 3, RNA-binding region (RNP1, RRM) containing 3, secreted and transmembrane 1, and serine palmitoyltransferase, small subunit B) were technically validated using quantitative real-time polymerase chain reaction (qRT-PCR) in order to check the accuracy of RNA-seq analysis. Finally, 83 gene ontology biological processes including several blood vessel and mammary gland development related terms, were significantly detected using DAVID gene-set enrichment analysis. From these results, we observed that detected milk production related genes are highly enriched in the circulation system process and mammary gland related biological functions. In addition, we observed that detected genes including caveolin 1, mammary serum amyloid A3.2, lingual antimicrobial peptide, cathelicidin 4 (CATHL4), cathelicidin 6 (CATHL6) have been reported in other species as milk production related gene. For this reason, we concluded that our detected 271 genes would be strong candidates for determining milk production.
Heavy metals in the mangrove sediments of Chuuk and Kosrae, Federated States of Micronesia were analyzed to examine the pollution levels of heavy metals using enrichment factor (EF) and pollution load index (PLI). The mean concentrations of Cr, Ni, Cu, Zn, As, Cd and Pb in surface mangrove sediments were 642, 125, 46.9, 149, 15.6, 0.14 and 8.55 ${\mu}g$, respectively. Kosrae mangrove sediments showed the highest concentrations of Cr and Ni while Chuuk contains more of other metals such as Cu, Zn, As, Cd and Pb. Compared to those from other mangrove regions of the world, Cr, Ni and As levels in mangrove sediments from Micronesia were at higher levels whereas Cu, Zn, Cd and Pb were at lower to median levels. In core sediment of Chuuk, metal concentrations in the upper part were higher than those in the lower part. Based on the EF and PLI values, As is evaluated as the heaviest contaminant in the surface sediment from Micronesia whilst other metals (Cr, Ni, Cu, Zn, Cd and Pb) are present at slightly lesser levels.
Roh, Seung-Young;Kim, Ji Yeon;Cha, Hyo Kyeong;Lim, Hye Young;Park, Youngran;Lee, Kwang-No;Shim, Jaemin;Choi, Jong-Il;Kim, Young-Hoon;Son, Gi Hoon
Molecules and Cells
/
v.43
no.4
/
pp.408-418
/
2020
The sinus node (SN) is located at the apex of the cardiac conduction system, and SN dysfunction (SND)-characterized by electrical remodeling-is generally attributed to idiopathic fibrosis or ischemic injuries in the SN. SND is associated with increased risk of cardiovascular disorders, including syncope, heart failure, and atrial arrhythmias, particularly atrial fibrillation. One of the histological SND hallmarks is degenerative atrial remodeling that is associated with conduction abnormalities and increased right atrial refractoriness. Although SND is frequently accompanied by increased fibrosis in the right atrium (RA), its molecular basis still remains elusive. Therefore, we investigated whether SND can induce significant molecular changes that account for the structural remodeling of RA. Towards this, we employed a rabbit model of experimental SND, and then compared the genome-wide RNA expression profiles in RA between SND-induced rabbits and sham-operated controls to identify the differentially expressed transcripts. The accompanying gene enrichment analysis revealed extensive pro-fibrotic changes within 7 days after the SN ablation, including activation of transforming growth factor-β (TGF-β) signaling and alterations in the levels of extracellular matrix components and their regulators. Importantly, our findings suggest that periostin, a matricellular factor that regulates the development of cardiac tissue, might play a key role in mediating TGF-β-signaling-induced aberrant atrial remodeling. In conclusion, the present study provides valuable information regarding the molecular signatures underlying SND-induced atrial remodeling, and indicates that periostin can be potentially used in the diagnosis of fibroproliferative cardiac dysfunctions.
Journal of Korean Society for Atmospheric Environment
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v.21
no.1
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pp.119-129
/
2005
The ambient TSP data measured at Seoul, Incheon. Taean, Daegu, Busan in Korea were used to explain the chemical composition and general features of Asian Dust (AD) observed in Korea. 9 episodes out of 19 were sampled from 2000 through May 2002, and measurements were conducted covering ionic and metal components with mass concentration. The results showed that daily averaged mass concentration (TSP) during the AD episodes was 458 $\mu\textrm{g}$/㎥, and ionic and metal concentrations were 27.93 $\mu\textrm{g}$/㎥ and 71.7 $\mu\textrm{g}$/㎥, respectively, accounting for 6.1 % and 15.5% of the total aerosol mass. TSP concentrations during episodes were varied from 120 to 1742 $\mu\textrm{g}$/㎥ according to the impact of Asian Dusts and had a tendency of showing higher values at sites in the west side of Korea, which can be explained by the effect of diffusion and deposition. In this study, ionic components like Ca (NO$_3$)$_2$, CaSO$_4$, NaNO$_3$, Na$_2$SO$_4$ were prominent types in secondary aerosol during AD periods and also indicated that V, Co as well as soil elements such as Ca, Fe, Mg, Mn, K correlated well with Al, while Cu, Cd, Pb, Zn didn't agree well with it. In addition, enrichment factors (EFs) for each metal component were obtained to provide simple information about source contribution of Asian Dust, and the results were compared with those from other AD studies. In this study, the results showed that aerosol properties in Korea during the Asian Dust were considerably different from those of general atmospheric condition and specially varied from case to case rather than site to site, which implies that there are certain variations in the soil of source region, pathways of air mass, and meteorological condition. For the enhanced study, those factors should be combined with the features of Asian Dust resolved from this study.
As the first comprehensive attempt at a national implementation, this study aims at assessing the external costs of major electricity generation technologies in Korea, particularly an evaluation of the impacts on human health resulting from exposures to atmospheric radiological emissions from nuclear power plants, and a monetary quantification of their damages. The methodology used for the assessment of the externalities of the selected fuel cycles has been developed by the International Atomic Energy Agency (IAEA), namely the SimPacts Model Package. The model is internationally recognized as a tool which can be applied to a wide range of fuels, different technologies and locations, for an externalities study. In this study, the relevant emissions are quantified first and then their impacts on human health are evaluated and compared. The study focused on all the nuclear power plants for the last 6 years ($2001{\sim}2006$) in Korea. With respect to nuclear power, the impact analysis only focuses on a power generation, however the front- and back-end nuclear fuel cycles are not included, namely uranium mining, conversion, enrichment, reprocessing, conditioning, etc., because these facilities are not present in Korea. The analysis results show that nuclear power in general, generates low external costs. The highest damage costs from the nuclear power plants among the 4 sites in Korea were estimated to be 3.9 mills/MWh, which is about 1/20th of the result for a similar case study conducted in the U.K., implemented through the ExternE project. This difference is largely due to the number of radionuclides included in the study and the amount of released radioactive emissions based on up-to-date information in Korea. In this study, the sensitivities of the major factors for nuclear power plants were also calculated. The analysis indicates that there was around a ${\pm}3%$ damage costs variation to a ${\pm}15%$ change of the reference population density and a ${\pm}1%$ damage cost variation to a $1{\sim}30$ meters change of the effective release height, respectively. These sensitive calculations show that there is only a minor difference when the reference costs are compared.
Mathematically gifted children have creative curiosity about novel tasks deriving from their natural mathematical talents, aptitudes, intellectual abilities and creativities. More effect in nurturing the creative thinking found in brilliant children, letting them approach problem solving in various ways and make strategic attempts is needed. Given this perspective, it is desirable to select open-ended and atypical problems as a task for educational program for gifted children. In this paper, various types of open-ended problems were framed and based on these, teaming activities were adapted into gifted children's class. Then in the problem solving process, the characteristic of bright children's mathematical thinking ability and examples of problem solving strategies were analyzed so that suggestions about classes for bright children utilizing open-ended tasks at elementary schools could be achieved. For this, an open-ended task made of 24 inquiries was structured, the teaching procedure was made of three steps properly transforming Renzulli's Enrichment Triad Model, and 24 periods of classes were progressed according to the teaching plan. One period of class for each subcategories of mathematical thinking ability; ability of intuitional insight, systematizing information, space formation/visualization, mathematical abstraction, mathematical reasoning, and reflective thinking were chosen and analyzed regarding teaching, teaming process and products. Problem solving examples that could be anticipated through teaching and teaming process and products analysis, and creative problem solving examples were suggested, and suggestions about teaching bright children using open-ended tasks were deduced based on the analysis of the characteristic of tasks, role of the teacher, impartiality and probability of approaching through reflecting the classes. Through the case study of a mathematics class for bright children making use of open-ended tasks proved to satisfy the curiosity of the students, and was proved to be effective for providing and forming a habit of various mathematical thinking experiences by establishing atypical mathematical problem solving strategies. This study is meaningful in that it provided mathematically gifted children's problem solving procedures about open-ended problems and it made an attempt at concrete and practical case study about classes fur gifted children while most of studies on education for gifted children in this country focus on the studies on basic theories or quantitative studies.
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