• Journal of Microbiology and Biotechnology
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• v.26 no.10
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• pp.1701-1707
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• 2016

Lipoxygenase (LOX) is an industrial enzyme with wide applications in food and pharmaceutical industries. The available structure information indicates that eukaryotic LOXs consist of N terminus β-barrel and C terminus catalytic domains. However, the latest crystal structure of Pseudomonas aeruginosa LOX shows it is significantly different from those of eukaryotic LOXs, including the N-terminal helix domain. In this paper, the functions of this N-terminal helix domain in the soluble expression and catalysis of P. aeruginosa LOX were analyzed. Genetic truncation of this helix domain resulted in an insoluble P. aeruginosa LOX mutant. The active C-terminal domain was obtained by dispase digestion of the P. aeruginosa LOX derivative containing the genetically introduced dispase recognition sites. This functional C-terminal domain showed raised substrate affinity but reduced catalytic activity and thermostability. Crystal structure analyses demonstrate that the broken polar contacts connecting the two domains and the exposed hydrophobic substrate binding pocket may contribute to the insoluble expression of the C terminus domain and the changes in the enzyme properties. Our data suggest that the N terminus domain of P. aeruginosa LOX is required for its soluble expression in E. coli, which is different from that of the eukaryotic LOXs. Besides this, this N-terminal domain is not necessary for catalysis but shows positive effects on the enzyme properties. The results presented here provide new and valuable information on the functions of the N terminus helix domain of P. aeruginosa LOX and further improvement of its enzyme properties by molecular modification.

• Journal of Korean Institute of Industrial Engineers
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• v.39 no.4
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• pp.239-246
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• 2013

Global restaurant franchises are becoming more and more popular in China, Japan, and South Korea. Understanding target customers' needs for restaurant franchises is considered one of the most important factors for their business success. In this study, four domains-food-related domain (FRD), interior-related domain (IRD), staff-related domain (SRD), and convenience-related domain (CRD)-and 24 elements were classified. A survey with 450 respondents in the three capital cities (Beijing, Tokyo, and Seoul) was then conducted to examine the effect of the factors influencing customer satisfaction. After statistical analysis, thirteen main elements were found as factors influencing customer satisfaction. The analysis result showed that the food-related domain (FRD) was the most important in Beijing. The interior-related domain (IRD) and the convenience-related domain (CRD) were considered the most important in Tokyo and Seoul. Some cultural differences in customer satisfaction were also found. The results provide a guideline for better customer experience management of a restaurant franchise in the three cities.

• Fisheries and Aquatic Sciences
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• v.26 no.3
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• pp.204-215
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• 2023

The thermolabile haemolysin (tlh) of Vibrio parahaemolyticus (Vptlh) from V. parahaemolyticus is a multiple-function enzyme, initially describes as a haemolytic factor activated by lecithin and phospholipase A2 enzymatic activity (Shinoda, 1991; Vazquez-Morado, 2021; Yanagase et al., 1970). Until now, the tlh structure has hypothesized including N-terminal and C-terminal domain, but what domain of the Vptlh structure does the haemolytic activity has not been refined yet. In this study, a 450-bp VpTLH nucleotide sequence of the entire Vptlh gene encoded the C-terminal domain cloned firstly to examine its responsibility in the activity of the Vptlh. The C-terminal domain fused with a 6-His-tag named the His-tag-VpC-terminal domain was expressed successfully in soluble form in the BL21 (DE3) PlysS cell. Remarkably, both expression and purification results confirmed a high agreement in the molecular weight of the His-tag-VpC-terminal domain was 47 kDa. This work showed the His-tag-VpC-terminal domain lysed the erythrocyte membranes in the blood agar and the phosphate buffered saline (0.9%) media without adding the lecithin substrate of the phospholipase enzyme. Haemolysis occurred at all tested diluted concentrations of His-tag-VpC-terminal domain (p < 0.05), providing evidence for the independent haemolytic activity of the His-tag-VpC-terminal domain. The content of 100 ㎍ of the His-tag-VpC-terminal domain brought the highest haemolytic activity of 80% compared to that in the three remaining contents. Significantly, the His-tag-VpC-terminal domain demonstrated not to involve the phospholipase activity in Luria-Bertani agar supplemented with 1% (vol/vol) egg yolk emulsion. All results proved the vital responsibility of the His-tag-VpC-terminal domain in causing the haemolytic activity without the required activation by the phospholipase enzyme. Raw extracts of Phellinus igniarus and Phellinus pipi at 10^-1 mg/mL inhibited the haemolytic activity of the His-tag-VpC-terminal domain from 67.7% to 87.42%, respectively. Hence applying the His-tag-VpC-terminal domain as a simple biological material to evaluate quickly potential derivatives against the Vptlh in vivo conditions will accessible and more advantageous than using the whole of the Vptlh.

• 한국HCI학회:학술대회논문집
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• 2006.02b
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• pp.76-82
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• 2006

본 연구는 항공기 관제사의 관제디스플레이에 대한 생태학적 인터페이스 디자인을 적용하기 위한 초기작업으로써 항공기 관제영역에 대한 Work Domain Analysis 를 실시하여 Work Domain Model 을 개발하고 모델에 대한 테스트를 통해 모델의 타당성을 검증하였다. 또한, 완성된 모델을 바탕으로 디자인을 위한 Information Requirement 를 추출함으로써 지금까지 적용해보지 않은 항공기 관제영역에 대한 생태학적 인터페이스 디자인 툴(Tool)의 새로운 적용가능성을 확인하여 추후, 구체적인 관제 디스플레이의 생태학적 개발에 기본 틀을 제공하고자 한다.

• Journal of Industrial Technology
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• v.22 no.B
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• pp.163-167
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• 2002

Autonomic nervous system play an important role of keeping our health as balancing homeostasis. But the abnormality of these abilities makes our presence be feeble. To obtain these information of body which helps for us to decide whether one is healthy or not, based on the study of Heart Rate Variability. In this paper, we presented HRV model and its processing steps to extract some information of human body. After that, some experimental results are presented in time-domain and frequency-domain.

• Journal of the Korean Society for Industrial and Applied Mathematics
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• v.24 no.2
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• pp.161-197
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• 2020

Total variation minimization is standard in mathematical imaging and there have been numerous researches over the last decades. In order to process large-scale images in real-time, it is essential to design parallel algorithms that utilize distributed memory computers efficiently. The aim of this paper is to illustrate recent advances of domain decomposition methods for total variation minimization as parallel algorithms. Domain decomposition methods are suitable for parallel computation since they solve a large-scale problem by dividing it into smaller problems and treating them in parallel, and they already have been widely used in structural mechanics. Differently from problems arising in structural mechanics, energy functionals of total variation minimization problems are in general nonlinear, nonsmooth, and nonseparable. Hence, designing efficient domain decomposition methods for total variation minimization is a quite challenging issue. We describe various existing approaches on domain decomposition methods for total variation minimization in a unified view. We address how the direction of research on the subject has changed over the past few years, and suggest several interesting topics for further research.

• Journal of the Korean Society for Industrial and Applied Mathematics
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• v.9 no.2
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• pp.99-106
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• 2005

We present a relation between the orthogonality of the constrained Legendre polynomials over the triangular domain and the BB ($B{\acute{e}zier}\;-Bernstein$) coefficients of the polynomials using the equivalence of orthogonal complements. Using it we also show that the best constrained degree reduction of polynomials in BB form equals the best approximation of weighted Euclidean norm of coefficients of given polynomial in BB form from the coefficients of polynomials of lower degree in BB form.

• Proceedings of the Korea Society for Industrial Systems Conference
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• 1999.05a
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• pp.80-83
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• 1999

본 논문에서는 서비스 컴포넌트 또는 서비스간의 기능 결합을 통한 서비스 기능 확장 및 신규 서비스 생성을 가능토록 하는 서비스 컴포지션 구조를 설계하였다. 설계된 컴포지션 구조는 TINA-C에서 제안한 서비스 구조를 기반으로 하고 있으며, 서비스 관리 영역 구분에 따라 두 서비스 제공자 영역에서 수행되는 Inter-Domain 컴포지션과 하나의 서비스 제공자 영역에서 수행되는 Intra-Domain 컴포지션으로 구분하여 설계 되었다.

• Journal of the Korean Society for Industrial and Applied Mathematics
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• v.26 no.4
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• pp.323-332
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• 2022

This paper presents a numerical study on multigrid algorithms of V-cycle type for problems posed in the Hilbert space H(curl) in three dimensions. The multigrid methods are designed for discrete problems originated from the discretization using the hexahedral Nédélec edge element of the lowest-order. Our suggested methods are associated with smoothers constructed by substructuring based on domain decomposition methods of nonoverlapping type. Numerical experiments to demonstrate the robustness and the effectiveness of the suggested algorithms are also provided.

• Proceedings of the KIEE Conference
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• 1996.07b
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• pp.731-733
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• 1996

In this paper, a new time-domain reduction method for unbalanced 3 phase power systems will be represented. The impulse response of the system is used to identify a discrete-time equivalent filter model. The model is formulated directly in the phase domain. Each phase has a self-mode equivalent model and two mutual-mode equivalent models. The equivalent model is determined by the transfer function identification technique based on the Prony analysis. The model is implemented in EMTDC and tested with an unbalanced 3 phase network. The result of test showed that the equivalent model is accurate.