• Title/Summary/Keyword: Induction effect

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Ginsenoside Rb2 suppresses cellular senescence of human dermal fibroblasts by inducing autophagy

  • Kyeong Eun Yang;Soo-Bin Nam;Minsu Jang;Junsoo Park;Ga-Eun Lee;Yong-Yeon Cho;Byeong-Churl Jang;Cheol-Jung Lee;Jong-Soon Choi
    • Journal of Ginseng Research
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    • v.47 no.2
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    • pp.337-346
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    • 2023
  • Background: Ginsenoside Rb2, a major active component of Panax ginseng, has various physiological activities, including anticancer and anti-inflammatory effects. However, the mechanisms underlying the rejuvenation effect of Rb2 in human skin cells have not been elucidated. Methods: We performed a senescence-associated β-galactosidase staining assay to confirm cellular senescence in human dermal fibroblasts (HDFs). The regulatory effects of Rb2 on autophagy were evaluated by analyzing the expression of autophagy marker proteins, such as microtubule-associated protein 1A/1B-light chain (LC) 3 and p62, using immunoblotting. Autophagosome and autolysosome formation was monitored using transmission electron microscopy. Autophagic flux was analyzed using tandem-labeled GFP-RFP-LC3, and lysosomal function was assessed with Lysotracker. We performed RNA sequencing to identify potential target genes related to HDF rejuvenation mediated by Rb2. To verify the functions of the target genes, we silenced them using shRNAs. Results: Rb2 decreased β-galactosidase activity and altered the expression of cell cycle regulatory proteins in senescent HDFs. Rb2 markedly induced the conversion of LC3-I to LC3-II and LC3 puncta. Moreover, Rb2 increased lysosomal function and red puncta in tandem-labeled GFP-RFP-LC3, which indicate that Rb2 promoted autophagic flux. RNA sequencing data showed that the expression of DNA damage-regulated autophagy modulator 2 (DRAM2) was induced by Rb2. In autophagy signaling, Rb2 activated the AMPK-ULK1 pathway and inactivated mTOR. DRAM2 knockdown inhibited autophagy and Rb2-restored cellular senescence. Conclusion: Rb2 reverses cellular senescence by activating autophagy via the AMPK-mTOR pathway and induction of DRAM2, suggesting that Rb2 might have potential value as an antiaging agent.

Effects of GA3 Dipping Treatment on the Spike Growth and Fruit Quality at Harvest of 'Campbell Early' Grapevine ('캠벨얼리' 포도의 화수(花穗) 생장과 과실품질에 미치는 GA3의 영향)

  • Lee, Y.C.;Moon, B.W.;Nam, K.W.;Moon, Y.J.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.15 no.1
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    • pp.85-93
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    • 2013
  • This study has been conducted to investigate the effect of GA3 solution dipping treatment after 1 days full bloom on spike growth and fruit quality at harvest of 'Campbell Early' grapevine. The fruit cluster growth(weight, length, width) and fruit stalk(diameter, length) at harvest were increased significantly by GA3 10 mg·L-1 solution dipping treatment compared to control and another concentration. The berry growth (berry No, weight, length, diameter) at harvest showed no difference by treatment GA3 concentration different. Spike weight was increased by GA3 5, 10, 20, mg·L-1 treatment compared to control, but no difference GA3 40 mg·L-1 treatment. The degree of compact berry, bloom, skin color and SSC at harvest by GA3 solution dipping treatment were not significantly different from those of control. but increased seedlessness induction by GA3 10 mg·L-1 treatment. The occurrence percent of berry cracking by GA3 5, 10, 20, 40 mg·L-1 treatment significantly decreased. The bitter rot of berry harvest showed no difference by treatment GA3 concentration different. Total spike length was increased by GA3 5, 10 mg·L-1 treatment compared to control and GA3 20, 40 mg·L-1 treatment. The spike length of 1st from 15th to position increased by GA3 10 mg·L-1 treatment.

Effect of GA3 Treatment on the Flowering in Tuber of Zantedeschia 'Black Magic' (GA3 처리가 유색칼라 괴경의 개화에 미치는 영향)

  • Park, Nou-Bog;Lim, Hoe-Chun
    • Journal of Practical Agriculture & Fisheries Research
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    • v.7 no.1
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    • pp.90-96
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    • 2005
  • Tubers(4-5cm diameter) of Zantedeschia 'Black Magic' were used to study the induction of flowering in GA3 soaking times and concentration. The GA3 soaking were 10 seconds and 30 minutes at the GA3 concentrations of 50, 100, 250, and 500 mg·L1. The time to emergence in GA3 treatment were 1.1~4.0 days shorter than control Growth characteristics were good in GA3 treatment but that was no significance in GA3 soaking time and concentration. When GA3 soaking time and concentration were increased, days to flowering was shorter, but flower stalk length, flower stalk width, flower length, flower width were no difference. The number of flower per tuber was most as 4.0~4.3 in 250~500 mg·L1 GA3 concentration and that was about 2 times compared to control. The normal flower ratio and bulb enlargement were similar compared with GA3 soaking time and concentration. GA3 250~500 mg·L1 treatment is necessary for improvement of number of flowers per bulb.

MicroRNA analysis reveals the role of miR-214 in duck adipocyte differentiation

  • Wang, Laidi;Hu, Xiaodan;Wang, Shasha;Yuan, Chunyou;Wang, Zhixiu;Chang, Guobin;Chen, Guohong
    • Animal Bioscience
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    • v.35 no.9
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    • pp.1327-1339
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    • 2022
  • Objective: Fat deposition in poultry is an important factor in production performance and meat quality research. miRNAs also play important roles in regulating adipocyte differentiation process. This study was to investigate the expression patterns of miRNAs in duck adipocytes after differentiation and explore the role of miR-214 in regulating carnitine palmitoyltransferases 2 (CPT2) gene expression during duck adipocyte differentiation. Methods: Successful systems for the isolation, culture, and induction of duck primary fat cells was developed in the experiment. Using Illumina next-generation sequencing, the miRNAs libraries of duck adipocytes were established. miRanda was used to predict differentially expressed (DE) miRNAs and their target genes. The expression patterns of miR-214 and CPT2 during the differentiation were verified by quantitative real-time polymerase chain reaction and western blot. Luciferase reporter assays were used to explore the specific regions of CPT2 targeted by miR-214. We used a miR-214 over-expression strategy in vitro to further investigate its effect on differentiation process and CPT2 gene transcription. Results: There were 481 miRNAs identified in duck adipocytes, included 57 DE miRNA candidates. And the 1,046 targets genes of DE miRNAs were mainly involved in p53 signaling, FoxO signaling, and fatty acid metabolism pathways. miR-214 and CPT2 showed contrasting expression patterns before and after differentiation, and they were selected for further research. The expression of miR-214 was decreased during the first 3 days of duck adipocytes differentiation, and then increased, while the expression of CPT2 increased both in the transcriptional and protein level. The luciferase assay suggested that miR-214 targets the 3'untranslated region of CPT2. Overexpression of miR-214 not only promoted the formation of lipid droplets but also decreased the protein abundance of CPT2. Conclusion: Current study reports the expression profile of miRNAs in duck adipocytes differentiated for 4 days. And miR-214 has been proved to have the regulator potential for fat deposition in duck.

Effects of Self-Directed and Unself-Directed Prior Learning on Student Attitude Towards Science Class (자기 및 타의주도적 선행학습이 과학 학습 태도에 미치는 효과)

  • Choi, Jeong-Seon;Park, Jong-Keun;Koo, In-Sun
    • Journal of The Korean Association For Science Education
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    • v.27 no.9
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    • pp.765-777
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    • 2007
  • Investigations into how the status of prior learning on the textbook of science were conducted, and the effects of prior learning on students' attitudes towards science class were carried out. The investigations for the status of prior learning were performed with a number of students who experienced prior learning, (self-directed and unself-directed prior learning), a selfless intention as the starting motive for prior learning, the problem solving strategies used by students in prior learning, and the important factor that influenced prior learning. The effects of prior learning on student attitude towards science class were also examined with respect to four categories, including confidence, interest, learning intention, and value (effect). The effects of prior learning on the four categories were analyzed on the basis of the students' level of scientific achievement, the types of prior learning, the starting motive for prior learning, and the extent of the students' understanding of the content on prior learning. The analytical results for the effects of prior learning on students' attitudes towards science class showed that the mean values of confidence, learning intention, and value among the students in the self-directed prior learning group were higher than those of the students in the unself-directed prior learning group. These findings are the result of positive recognition, such as the possibility that the students experienced with self-directed prior learning can do, the induction of an inherent motive based on their level of achievement in science class, and the operant learning of strategies for solving problems in science class. Meanwhile, by the effects of having a teacher lead the science class and the consensus formed between friends, the mean level of interest was higher in the unself-directed prior learning than in the self-directed prior learning.

Evaluation of Biological Activity of Veronica incana Extracts (Veronica incana 추출물의 생물학적 활성 평가)

  • Mi-Rae Shin;Mi Yeong Yoon;Min Ju Kim;Il-Ha Jeong;Hui Yeon An;Ji-Won Jung;Seong-Soo Roh
    • The Korea Journal of Herbology
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    • v.39 no.3
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    • pp.57-67
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    • 2024
  • Objectives : The aim of this study is to evaluate the potential biological activity of Veronica incana extracts (VIE) through in vitro, ex vivo, and in vivo experiments. Methods : In vitro, we conducted analyses on the total polyphenol (TP) and total flavonoid (TF) levels, alongside DPPHand ABTS radical scavenging activities. Ex vivo evaluations on adipose tissue measured glycerol release as a marker of lipolysis. In LPS-induced RAW 264.7 cells, we quantified nitric oxide (NO) production. Following H2O2 induction in U2OS cells, we performed mitochondrial assays such as MitoSox and MitoTracker. Moreover, Bodipy assays were conducted in 3T3-L1 cells. In vivo, we performed anti-osteoarthritis effect of VIE against monosodium iodoacetate (MIA)-induced osteoarthritis in rats. Results : The results presented encompass a myriad of models, from cell culture to animal experiments as well as ex vivo studies. VIE demonstrated high TP and TF contents, potent DPPH and ABTS scavenging activities, and regulated glycerol release. Moreover, the inhibition of NO production in LPS-induced inflammation was notably confirmed and the reduction of lipid droplets was distinctly shown. Furthermore, in H2O2-induced U2OS cells, MitoSox was effectively reduced while MitoTracker noticeably increased. In vivo assays confirmed a significant increase in hindpaw weight distribution (HWD) decreased by MIA after VIE treatment. Additionally, VIE inhibited serum inflammatory cytokines (TNF-𝛼, IL-6, and IL-1𝛽) and MDA levels in joint tissue. Conclusion : In conclusion, Veronica incana exhibited various pharmacological effects including antioxidant, anti-obesity, and anti-inflammatory properties.

The Impact of Awe on Preference for Innovative Products: The Mediated Moderating Effect of Positive Technology Readiness (경외감이 혁신적 제품 선호도에 미치는 영향: 긍정적 기술준비도의 매개된 조절효과 )

  • Ga Young Lim
    • Knowledge Management Research
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    • v.24 no.4
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    • pp.87-102
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    • 2023
  • Awe is recognized as an emotion linked to enhancing openness to new experiences and broadening one's cognitive perspective through the acquisition of new knowledge. This research aimed to investigate awe's impact on consumer behavior in advertising, using a 2 (emotion: awe/control) × 2 (product type: innovative/non-innovative) experimental design with 118 undergraduate students in Seoul, Korea. Findings revealed that awe-inducing advertising significantly increases product preference and positive technology readiness, particularly for innovative products compared to non-innovative ones or when awe is not elicited. The analysis of moderated mediation showed that positive technology readiness plays a mediating role in the relationship between awe induction and product preference. Notably, in the case of innovative products, awe-inducing advertising heightens positive technology readiness, subsequently increasing consumer preference for these products. These results reinforce existing literature on awe's positive effects, demonstrating its role in augmenting consumers' favorable attitudes toward innovative products. The study offers valuable insights for marketing strategies of companies promoting innovative products or services, highlighting the effectiveness of awe-inducing emotional appeals in shaping consumer attitudes towards innovation.

Cellular Aging Inhibitory Effect of Perilla Leaf Extract on D-Galactose Induced C2C12 Myoblasts (D-갈락토스 유도 C2C12 근원세포에 대한 자소엽 추출물의 세포 노화 억제 효과)

  • Song-Mi Park;Sung-Woo Cho;Yung-Hyun Choi
    • Journal of Korean Medicine Rehabilitation
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    • v.34 no.2
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    • pp.15-28
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    • 2024
  • Objectives We used the D-galactose (D-gal) induced C2C12 myoblast senescence model to investigate whether ethanol extract of Perilla. fructescens leaves (EEPF) could delay cellular senescence and regulate related mechanisms. Methods C2C12 myogenic cells were cultured in an incubator under 37 ℃ and 5% CO2 conditions. EEPF, dried perilla leaves were pulverized and extracted at 1:10 (v/v) at 50 ℃ for 4 hours. Cell counting kit-8 and western blot analysis was performed. Annexin V-FITC apoptosis detection kit and DAPI staining was applied. Catalase (CAT), glutathione peroxidase (GSH-Px), total antioxidant capacity (T-AOC), superoxide dismutase (SOD), and malondialdehyde analysis kits were used. To measure the level of reactive oxygen species generation, staining and flow cytometry was used. To analyze the mitochondrial activity, membrane potential changes were measured using JC-1. 𝛽-gal activity was analyzed using SA-𝛽-gal staining solution, and DNA damage was analyzed by using 𝛾-H2AX. Quantikine ELISA kit was used to analyze inflammatory cytokine production. Results According to the results of this study, EEPF significantly alleviated the decrease in cell viability in C2C12 cells treated with D-gal and suppressed the decrease in the expression of proliferating cell nuclear antigen. EEPF also markedly blocked D-gal-induced C2C12 cell apoptosis and restored reduced activity of CAT, GSH-Px, T-AOC, SOD. In addition, EEPF suppressed the decrease in 𝛽-galactosidase activity, the induction of DNA damage and the increase in expression of senescence-associated secretory phenotype proteins such as p16, p53 and p21 in D-gal-treated C2C12 cells. Furthermore, EEPF significantly attenuated D-gal-induced production and expression of inflammatory cytokines such as interleukin (IL)-6 and IL-18. Conclusions The results of this study indicate that EEPF can be used as a potential candidate for the prevention and treatment of muscle aging.

Morroniside Protects C2C12 Myoblasts from Oxidative Damage Caused by ROS-Mediated Mitochondrial Damage and Induction of Endoplasmic Reticulum Stress

  • Hyun Hwangbo;Cheol Park;EunJin Bang;Hyuk Soon Kim;Sung-Jin Bae;Eunjeong Kim;Youngmi Jung;Sun-Hee Leem;Young Rok Seo;Su Hyun Hong;Gi-Young Kim;Jin Won Hyun;Yung Hyun Choi
    • Biomolecules & Therapeutics
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    • v.32 no.3
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    • pp.349-360
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    • 2024
  • Oxidative stress contributes to the onset of chronic diseases in various organs, including muscles. Morroniside, a type of iridoid glycoside contained in Cornus officinalis, is reported to have advantages as a natural compound that prevents various diseases. However, the question of whether this phytochemical exerts any inhibitory effect against oxidative stress in muscle cells has not been well reported. Therefore, the current study aimed to evaluate whether morroniside can protect against oxidative damage induced by hydrogen peroxide (H2O2) in murine C2C12 myoblasts. Our results demonstrate that morroniside pretreatment was able to inhibit cytotoxicity while suppressing H2O2-induced DNA damage and apoptosis. Morroniside also significantly improved the antioxidant capacity in H2O2-challenged C2C12 cells by blocking the production of cellular reactive oxygen species and mitochondrial superoxide and increasing glutathione production. In addition, H2O2-induced mitochondrial damage and endoplasmic reticulum (ER) stress were effectively attenuated by morroniside pretreatment, inhibiting cytoplasmic leakage of cytochrome c and expression of ER stress-related proteins. Furthermore, morroniside neutralized H2O2-mediated calcium (Ca2+) overload in mitochondria and mitigated the expression of calpains, cytosolic Ca2+-dependent proteases. Collectively, these findings demonstrate that morroniside protected against mitochondrial impairment and Ca2+-mediated ER stress by minimizing oxidative stress, thereby inhibiting H2O2-induced cytotoxicity in C2C12 myoblasts.

Immune stimulating effects of Astragalus membranaceus and Zanthoxylum schinifolium 1:1 mixture in Raw264.7 cells (Raw264.7 세포에서 황기와 산초 1:1 혼합물의 면역 증진 효과)

  • Il Je Cho;Yeong Eun Yu;Sang Min Lee;Eun Ok Kim;Joon Heum Park;Sea Kwang Ku
    • Journal of Applied Biological Chemistry
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    • v.66
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    • pp.519-526
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    • 2023
  • Present study explored immunostimulatory effects of Astragalus membranaceus and Zanthoxylum schinifolium 1:1 (w:w) mixture (AZM-1:1) in Raw264.7 cells, mouse macrophage derived cells. Treatment with 100-400 ㎍/mL of AZM-1:1 in Raw264.7 cells significantly increased nitric oxide production in parallel with inducible nitric oxide synthase mRNA expression without affecting cytotoxicity. In addition, AZM-1:1 dose-dependently increased prostaglandin E2 production in conditioned medium along with cyclooxygenase-2 mRNA induction. Moreover, AZM-1:1 induces the transcription of tumor necrosis factor-α, interleukin-1β, interleukin-6, and monocyte chemoattractant protein-1. Immunoblot analyses revealed that AZM-1:1 significantly increased the phosphorylation of mitogen-activated protein kinases, provoked phosphorylation-mediated degradation of inhibitory-κBα, and phosphorylated p65. Furthermore, treatment with AZM-1:1 promoted phagocytosis of Escherichia coli particle labeled with green fluorescence. Taken together, AZM-1:1 may be a promising nutraceutical for stimulation the innate immune system, including macrophages.