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Evaluation of Biological Activity of Veronica incana Extracts

Veronica incana 추출물의 생물학적 활성 평가

  • Mi-Rae Shin (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Mi Yeong Yoon (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Min Ju Kim (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Il-Ha Jeong (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Hui Yeon An (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Ji-Won Jung (Department of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Seong-Soo Roh (Department of Herbology, College of Korean Medicine, Daegu Haany University)
  • 신미래 (대구한의대학교 한의학과 본초약리학교실) ;
  • 윤미영 (대구한의대학교 한의학과 본초약리학교실) ;
  • 김민주 (대구한의대학교 한의학과 본초약리학교실) ;
  • 정일하 (대구한의대학교 한의학과 본초약리학교실) ;
  • 안희연 (대구한의대학교 한의학과 본초약리학교실) ;
  • 정지원 (대구한의대학교 한의학과 본초약리학교실) ;
  • 노성수 (대구한의대학교 한의학과 본초약리학교실)
  • Received : 2024.04.15
  • Accepted : 2024.05.25
  • Published : 2024.05.30

Abstract

Objectives : The aim of this study is to evaluate the potential biological activity of Veronica incana extracts (VIE) through in vitro, ex vivo, and in vivo experiments. Methods : In vitro, we conducted analyses on the total polyphenol (TP) and total flavonoid (TF) levels, alongside DPPHand ABTS radical scavenging activities. Ex vivo evaluations on adipose tissue measured glycerol release as a marker of lipolysis. In LPS-induced RAW 264.7 cells, we quantified nitric oxide (NO) production. Following H2O2 induction in U2OS cells, we performed mitochondrial assays such as MitoSox and MitoTracker. Moreover, Bodipy assays were conducted in 3T3-L1 cells. In vivo, we performed anti-osteoarthritis effect of VIE against monosodium iodoacetate (MIA)-induced osteoarthritis in rats. Results : The results presented encompass a myriad of models, from cell culture to animal experiments as well as ex vivo studies. VIE demonstrated high TP and TF contents, potent DPPH and ABTS scavenging activities, and regulated glycerol release. Moreover, the inhibition of NO production in LPS-induced inflammation was notably confirmed and the reduction of lipid droplets was distinctly shown. Furthermore, in H2O2-induced U2OS cells, MitoSox was effectively reduced while MitoTracker noticeably increased. In vivo assays confirmed a significant increase in hindpaw weight distribution (HWD) decreased by MIA after VIE treatment. Additionally, VIE inhibited serum inflammatory cytokines (TNF-𝛼, IL-6, and IL-1𝛽) and MDA levels in joint tissue. Conclusion : In conclusion, Veronica incana exhibited various pharmacological effects including antioxidant, anti-obesity, and anti-inflammatory properties.

Keywords

Acknowledgement

이 성과물은 정부 (과학기술정보통신부, No.2018R1A5A2025272; 교육부, 2021R1I1A1A01059605)의 재원으로 한국연구재단의 지원을 받아 수행되었습니다.

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