• Title/Summary/Keyword: Induction effect

검색결과 2,984건 처리시간 0.033초

Aspirin이 출혈성 쇼크로 인한 급성 폐손상에 미치는 효과 (Aspirin Reduces Acute Lung Injury in Rats Subjected to Severe Hemorrhage)

  • 신태림;이동욱;박윤엽
    • Tuberculosis and Respiratory Diseases
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    • 제54권5호
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    • pp.522-531
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    • 2003
  • 연구배경 : 급성 폐손상을 일으키는 요인중의 하나인 출혈성쇼크시 나타나는 급성 폐손상의 양상과 아스피린이 이에 미치는 효과를 알아보고자 본 연구를 시행하였다. 방법 : 실험동물은 체중 350g 내외의 Sprague-Dawley 종흰쥐를 사용하였고, 혈압측정 및 출혈을 시키기 위하여 catheter를 양쪽 대퇴동맥에 삽입하였다. 수술 후 polygraph를 이용하여 평균동맥압을 기록하였으며, 출혈은 withdrawal pump를 이용하여 5분간 체중 kg 당 20 ml의 혈액을 출혈시켰다. 실험군은 대조군, 출혈군과 아스피린 처치군으로 분류하였다. 대조군은 출혈군과 동일하게 수술하고 출혈은 시키지 않았으며 나머지 과정은 출혈군과 동일하게 처리하였다. 아스피린 처치군은 출혈 30분전 대퇴정맥으로 아스피린 (10mg/kg)을 주입하였고, 출혈군은 체중 당 동일한 양의 생리식염수를 주입하였다. 이상의 처치 후 2시간 또는 24시간 동안 cage에서 자유롭게 활동하도록 하였으며, 출혈후의 폐손상 정도와 아스피린이 이에 미치는 효과를 알아보기 위하여 폐장내 myeloperoxidase 활성도와 폐세척액 내의 단백함량과 백혈구 수를 측정하였다. 결과 : 폐장내 myeloperoxidase 활성도와 폐세척액 내의 단백함량과 백혈구수는 출혈 2시간 및 24시간 후 대조군에 비해 유의하게 증가하였다. 이러한 반응은 아스피린 전처치에 의하여 효과적으로 차단되었다. 결론 : 이상의 결과로 심한 출혈 후에 급성 폐손상이 생길 수 있으며 이는 아스피린 전처치로 효과적으로 예방될 수 있다고 생각된다.

불등가사리 분획물의 암세포 성장 억제 효과 (Growth Inhibition Effects of Cancer Cell Lines by Gloiopeltis furcata Fractions in Vitro)

  • 박성영;정복미;최영현;배송자
    • 한국식품영양과학회지
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    • 제34권6호
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    • pp.771-775
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    • 2005
  • 본 실험은 홍조해조류의 하나인 불등가사리를 추출 후 각 용매별로 분획하여 암세포 성장억제 효과와 QR유도활성 효과 등 생리활성을 연구하였다. 불등가사리를 이용하여 4종의 인체 암세포주HepG2, HeLa, MCF-7및 HT-29와 정상 간세포에 대한 암세포 증식억제 실험을 한 결과 사용한 4종의 암세포주에서 모두 시료첨가 농도에 의존적으로 증식 저지 효과가 나타났고, 특히 불등가사리의 methanol 분획층인 GFMM에서는 매우 낮은 농도의 시료첨가에도 불구하고 괄목할 만한 높은 암세포 성장 억제효과를 나타내었으며, 정상 간세포에서는 모든 분획층에서 약한 세포증식 억제율을 나타내어 정상 세포에 미치는 독성이 낮음을 확인하였다. 특히 본 시료는 여성암의 대표적인 유방암과 자궁경부암의 암세포 성장저지효과가 탁월하였다. 한편, 사용한 4가지 암세포주 중 유일하게 QR을 가지고 있는 HepG2를 이용한 암 예방지표인 QR 효소 유도 활성 여부를 측정한 결과 분획물 첨가농도를 10, 20, 30 및 40 $\mu$g/mL로 첨가하였을 때 GFMM의 첨가농도 $20\mu g/mL$에서 대조군에 비해 약 2배 이상의 높은 QR 유도효과를 나타내어 최종농도 $40\mu g/mL$에서는 2.6의 암 예방 QR 유도효과를 나타내었다.

방사선 조사선량에 따른 유지의 이화학적 성질변화 (I) (Changes of the phsico-Chemical Characteristics of oils treated by the ${\gamma}$-ray irradiation (I) -The Extracted soybean oil-)

  • 임국이
    • 대한가정학회지
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    • 제30권4호
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    • pp.77-88
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    • 1992
  • To investigate the oxidative stabilities of the ${\gamma}$-ray irradiated soybean during storage and heating and some physico-chemical characteristics of soybean and the extracted soybean oil (SBO) with/without the ${\gamma}$-ray irradiation were determined. The ${\gamma}$-ray level use in irradiation for soybean were 2.5, 5.0 and 10.0 KGY respectively and Acid Value, Peroxide Value, Conjugated Diene Value, Composed Fatty Acids amounts, and Trans Fatty Acid occurrence were determined for all samples, which were incubated at 45$\pm$1$^{\circ}C$ for 25 days heated at 180$\pm$1$^{\circ}C$ for 30 hours. And these values of the ${\gamma}$-ray treated samples were compared to those of nontreated samples. The results were obtained as follows : 1. According to the increased level of the ${\gamma}$-ray irradiation, there was little difference in Dielectric Constant, Viscosity, and the Induction Period by Rancimat. But, in case of 5.0 KGY, oxidative stability was increased more twice than that of non-irradiation. In the quantity of fatty Acids composition of the extracted soybean oil irradiated with 10.0 KGY, palmitic, oleic and linoleic acids were less increased thanb those of non-irradiation, while stearic, linolenic acids were decreased. In the case of 2.5 KGY irradiation, stearic and oleic acids were increased. 2. The Acid Value of SBO according to the ${\gamma}$-ray irradiation level was almost not change, but was 0.1 lower than that of non-irradiation during incubation (45$\pm$1$^{\circ}C$). The Peroxide Value of SBO with the ${\gamma}$-ray irradiation, was very lower than that of non-irradiation, but its effect on oxidative stability was better of SBO treated with 5.0 KGY and 10.0 KGY. In the Fatty Acids composition of SBO, palmitic, stearic, oleic acids were increased, while linoleic, linolenic acids were decreased during incubation(45$\pm$1$^{\circ}C$). This tendency was more obvious due to the ${\gamma}$-ray level. While heating(180$\pm$1$^{\circ}C$), the Acid Value of SBO treated with the ${\gamma}$-ray irradiation was decreased, the Acid Value of SBO irradiated with 2.5 KGY was the lowest. Also the peroxide Values of SBO treated with 5.0 KGY, 10.0 KGY were very lower than that of non-irradiation. Conjugated Diene Value of SBO was almost unchanged according to the ${\gamma}$-level and heating time. 3. When the methyl linoleate was irradiated with the ${\gamma}$-ray, the Trans Fatty Acid was little produced. In case of SBO with non-irradiation, the trans C18:1 was occured about 6.5~7.9%, but trans C18:2 and C18:3 were not shown, while SBO irradiated with the ${\gamma}$-ray 2.5, 5.0, 10.0 KGY, trans C18:3 and C18:2 amount in SBO were increased according to heating time, but trans C18:3 was little occured. As these results, the effects of the ${\gamma}$-ray irradiation to oil containing food were to cut down the energy for food storage and to increase oxdative stability during storge. And also it was shown to be the best that 10.0 KGY of the ${\gamma}$-ray irradiation would be applied to soybean.

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Exploration of Functional Materials from Oriental Medicine Extracts Cultured with Tricholoma Matsutake Mycelium - (2) Effect of Extracts on Blood Glucose and Liver Function in Streptozotocin-Induced Diabetic Rat -

  • Kim, Hae-Ja;Choi, Yun-Hee;Cho, Hwa-Eun;Hong, Hak-Gi;Han, Jung-Ho;Lee, Ki-Nam
    • 대한예방한의학회지
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    • 제12권2호
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    • pp.13-25
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    • 2008
  • The purpose of this study was to investigate extract from mixed culture with Trichloloma matsutake mycelium in oriental medicine and cereal medium(OCM) to develop new material for pharmaceutical products and medicinal food for diabetes mellitus. To evaluate of hypoglycemic activity of OCM extracts, we examined the inhibitory activity of ${\alpha}$-glucosidasein OCM, blood glucose level and liver function of streptozotocin(STZ) induced diabetic rat. Experimental group was divided into 6 groups: first, it was divided into normal control group(hereafter NC group) and diabetes-induced group, and diabetes-induced group was subdivided into diabetic control group(DC group), treated by hot water extracts group(HE), ultra sonic waves, micro waves, and micro bubble extracts g roup(UE), crude polysaccharide of HE group (HEE) and crude polysaccharide of UE group(UEE) at a dose of 300mg/kg/body weight, respectively. In diabetic-induced groups, after streptozotocin was melted in 0.01M citrate buffer at 50mg/kg/body weight, when the non-fasting blood glucose levelwas 300 mg/dl or more in blood collected from the tail vein, it was regarded as diabetic induction and then such diabetic-induced experimental animals were used in this experiment. At the end of the experiment, blood glucose level increased by 4.19% in DC group but significantly decreased by 32.34%, 19.19%, 17.81% and 17.64%, respectively in UEEE, UE, HE, and HEE groups. In the cases of AST, ALT, and ALP, the experiment group treated with extracts showed significantly lowerblood glucose level than DC group. The levels of BUN and uric acid were found to be lower in the UMPM extract group(UE) than HW extract group(HE), which implies that herb medicine medium extracts in which Tricholoma matsutake mycelia were cultured are effective in reducing impaired liver function as well as high blood glucose level caused by diabetes. In addition, the administration of low temperature UMPM extracts was found to produce better results than that of high temperature hot water extracts. In this regard, it is expected that extracts from herb medicine obtained by cultivating Tricholoma matsutake mycelia will be widely used as new ingredients for foods and medicines for prevention and treatment of diabetes.

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Endoplasmic Reticulum (ER) Stress and Apoptosis in Parthenogenetic Porcine Embryos following Different Combination of Activation Methods

  • Park, Hye-Bin;Park, Yeo-Reum;Lee, Hwa-Yeon;Bae, Hyo-Kyung;Lee, Seunghyung;Park, Choon-Keun;Yang, Boo-Keun;Cheong, Hee-Tae
    • 한국수정란이식학회지
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    • 제32권1호
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    • pp.25-31
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    • 2017
  • This study was conducted to investigate the effect of activation method on the endoplasmic reticulum (ER) stress induction, apoptosis and in vitro development of porcine parthenogenetic embryos. Porcine in vitro matured oocytes were activated by four activation methods; 1) electric stimulus (ES) (E), 2) $ES+10{\mu}M$ Ca-ionophore (A23187) treatment (EC), 3) ES+2 mM 6-dimethylaminopurine (6-DMAP) treatment (ED), or 4) ES+A23187 and 6-DMAP treatments (ECD). Parthenogenetic embryos were sampled to analyze x-box binding protein 1 (Xbp1) mRNA, ER stress-associated genes and apoptosis genes at 3 h after ES and the 1-cell and blastocyst stages. In the EC group, the band intensity of spliced Xbp1 (Xbp1s) mRNA was higher than those of the other groups at the 3 h and 1-cell stage, and higher than that of the E group at the blastocyst stage. Four ER stress-associated genes were expressed at the highest level in the EC group and weakly expressed in the ED group at 3 h after activation. However, most of the genes were highly expressed at the 1-cell and blastocyst stages with some variation in the EC and ECD groups. Expression of Bcl-2-associated X protein (Bax) and caspase-3 mRNA was significantly higher in the EC group than in the other groups at all development stages. The developmental rates to the blastocyst stage were higher in the ED and ECD groups than in the E and EC groups. These results suggest that the intracellular ER stress of parthenogenetic porcine embryos is affected by the activation method and subsequently lead to the apoptosis of embryos.

Transition Metal Induces Apoptosis in MC3T3E1 Osteoblast: Evidence of Free Radical Release

  • Chae, Han-Jung;Chae, Soo-Wan;Kang, Jang-Sook;Yun, Dong-Hyeon;Bang, Byung-Gwan;Kang, Mi-Ra;Kim, Hyung-Min;Kim, Hyung-Ryong
    • The Korean Journal of Physiology and Pharmacology
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    • 제4권1호
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    • pp.47-54
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    • 2000
  • Transition metal ions including $Se^{2+},\;Cd^{2+},\;Hg^{2+}\;or\;Mn^{2+}$ have been thought to disturb the bone metabolism directly. However, the mechanism for the bone lesion is unknown. In this study, we demonstrated that MC3T3E1 osteoblasts, exposed to various transition metal ions; selenium, cadmium, mercury or manganese, generated massive amounts of reactive oxygen species (ROS). The released ROS were completely quenched by free radical scavengers-N-acetyl cysteine (NAC), reduced glutathione (GSH), or superoxide dismutase (SOD). First, we have observed that selenium $(10\;{\mu}M),$ cadmium $(100\;{\mu}M),$ mercury $(100\;{\mu}M)$ or manganese (1 mM) treatment induced apoptotic phenomena like DNA fragmentation, chromatin condensation and caspase-3-like cysteine protease activation in MC3T3E1 osteoblasts. Concomitant treatment of antioxidant; N-acetyl-L-cysteine (NAC), reduced-form glutathione (GSH), or superoxide dismutase (SOD), prevented apoptosis induced by each of the transition metal ions. Catalase or dimethylsulfoxide (DMSO) has less potent inhibitory effect on the apoptosis, compared with NAC, GSH or SOD. In line with the results, nitroblue tetrazolium (NBT) stain shows that each of the transition metals is a potent source of free radicals in MC3T3E1 osteoblast. Our data show that oxidative damage is associated with the induction of apoptosis in MC3T3E1 osteoblasts following $Se^{2+},\;Cd^{2+},\;Hg^{2+}\;or\;Mn^{2+}$ treatment.

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조직공학적 골재생을 위한 탈미넬화된 골분을 함유한 다공성 지지체의 제조 및 그 특성 (Preparation and Characterization of Demineralized Bone Particle-loaded PLGA Scaffold for Tissue Engineered Bone)

  • 장지욱;이봉;한창환;김문석;조선행;이해방;강길선
    • 폴리머
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    • 제28권5호
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    • pp.382-390
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    • 2004
  • 생체적합성 천연재료 중 하나인 탈미네랄화된 골분 (demineralized bone particle, DBP)은 골형성단백질 (BMP)을 함유하고 있어 골수간엽줄기세포 (BMSCs)의 분화를 유도한다. 본 연구에서는 DBP를 함유한 폴리 락타이드 (PLA)와 락타이드-글리콜라이드 공중합체 (PLGA) 다공성 지지체를 용매 캐스팅/염추출법으로 제조하였고, 수은다공측정계 및 전자주사현미경을 이용하여 특성결정 하였다. BMSCs는 골분화 배지를 이용하여 조골세포로 분화시켜 Wright-Giemsa, Alizarin red, von Kossa 및 ALP 염색으로 확인하였다. DBP가 함유된 지지체와 DBP가 함유되지 않은 지지체에 BMSCs를 파종한 후 면역결핍 누드마우스의 피하에 삽입하여 이들의 골형성 정도를 비교하여 보았다. 제조한 지지체의 다공도는 $90.2\%$ 이상이었고 평균 다공크기도 69.1$\mu$m 이상이었다. BMSCs는 Wright-Giemsa, Alizarin red, von Kossa 및 ALP 염색결과 조골세포로 분화가 가능했으며, 동물실험을 수행한 결과 DBP가 함유된 지지체에서 칼슘침착 영역을 확인할 수 있었지만 DBP가 함유되지 않은 지지체에서는 칼슘침착 영역을 확인하지 못하였다. 결론적으로 DBP를 함유한 지지체에서 DBP와 BMSCs가 골형성에 중요한 요인으로 작용한다고 사료된다.

Colchicine과 EMS 처리에 의한 고추냉이의 변이체 유도 (Effects of Colchicine and EMS on Induction of Variants in Wasabia japonica MATSUM)

  • 박기인;윤명자;권성환;최선영;이강수
    • 한국약용작물학회지
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    • 제4권1호
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    • pp.12-18
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    • 1996
  • Wasabia japonica종자의 생장조절물질에 의한 발아율 돌연변이 유도, 유도체의 핵형 분석과 isozyme pattern을 조사한 결과는 다음과 같다. 1) 고추냉이의 종자 발아는 $GA_3$ 100ppm 처리 시 36%, BAP 10ppm 처리 시 32%, $GA_3$ 100ppm+BAP 10ppm 조합 처리 시 65%의 높은 발아율을 나타냈다. 2) colchicine과 EMS처리로 인한 돌연변이 유도에 따른 생존율은 발아전 처리의 경우 colchicine 0.5%일때 62% 생존율을 보이며, 발아 후 처리는 colchicine 0.5% 일때 85%, EMS 0.06% 일때 55%의 생존율을 보였다. 3) colchicine과 EMS처리로 유도된 식물체의 핵형 분석은 colchicine 0.5%처리로 유도된 식물체에서 다수의 4배체를 관찰할 수 있있으며, EMS 0.06% 처리의 유도체에서는 정상 배수체만이 관찰되었다. 4) 돌연변이원으로 유도된 식물체의 peroxidase pattern은 colchicine 처리 식물체가 정상체와 다른 양상 (1 band)을 보이는 반면, EMS처리 식물체는 정상체와 같은 양상 (3 band)을 보였다.

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소아 급성 임파구성 백혈병에서 예방적 전뇌 방사선조사의 효과 (Effect of Prophylactic Cranial Irradiation in Acute Lymphoblastic Leukemia in Children)

  • 김일한;최두호;김종훈;하성환;박찬일;안효섭
    • Radiation Oncology Journal
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    • 제7권2호
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    • pp.269-277
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    • 1989
  • 1979년 8월부터 1986년 12월까지 관해유도 치료 후 골수 관해(M1)가 온 134예의 소아 급성 임파구성 백혈병 환아가 18Gy또는 24Gy의 전뇌 방사선 조사와 척수강내 methotrexate주입의 예방적 중추신경계 치료를 받았다. 중추신경계에서 처음 재발한 환자는 14.2%(19/134)였고 이중 증추신경계에서만 재발한 예는 5.2%(7/134), 골수 또는 고환에서의 재발과 동시에 재발한 예는 9%(12/134)였다. 남아와 연령이 많을수록 중추신경계 재발이 높았으며 진단당시 말초혈액내의 백혈구수가 50,000/ul이상인 예의 재발율이 높았다. 방사선 조사량 24Gy에서의 중추 신경계 재발율이 18Gy인 경우보다 높았으나 유의하지는 않았다. 전체 중추신경계 재발예 중 89%가 예방적 중추신경계 치료개시 후 4년 이내에 발생하였으며, 중추신경계에서만 재발예는 100%가, 중추신경계와 골수 또는 고환의 재발과 동시에 재발한 예의 83%가 각각 관찰되었다. 재발의 위험이 있는 환자를 감안한전체 중추신경계 재발율은 유지 화학요법 기간 중에는 11.9%였고 유지 화학 요법 종료 이후의 기간에서는 4.9%였다.

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Anti-atherosclerotic Effect of the Methanol Extract of Sorbus commixta Cortex in the High Cholesterol-Diet Rats

  • Kang, Dae-Gill;Sohn, Eun-Jin;Kim, Jin-Sook;Lee, Yun-Jung;Moon, Mi-Kyoung;Lee, An-Sook;An, Jun-Seok;Lee, Ho-Sub
    • 동의생리병리학회지
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    • 제20권5호
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    • pp.1337-1345
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    • 2006
  • Hypercholesterolemia is a pivotal pathogenic factor for the development and maintenance of atherosclerosis. The present study was designed to evaluate whether the methanol extract of Sorbus commixta cortex (MSC) restores vascular dysfunction in association with the aortic expressions of proinflarnmatory and adhesion molecules in high cholesterol (HC) diet-rats. Chronic treatment with low (100 mg/kg/day) or high doses (200 mg/kg/day) of MSC lowered the increase in plasma levels of triglyceride (TG) and low-density lipoprotein (LDL) cholesterol induced by a cholesterol-enriched diet without affecting on the plasma level of high density lipoprotein (HDL)-cholesterol. Vascular tone attenuated in the HC-diet rats was restored by administration with MSC. Treatment with MSC also suppressed the HC-induced increase in the monocyte chemoattractant protein-1 (MCP-1) and nuclear factor-$_K$B (NF-$_K$B) p65 expressions as well as expressions levels of adhesion molecules including intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (ICAM-1), and E-selectin in aorta. The present study also showed that MSC inhibited the HC-mediated induction of ET-1 and ACE expression. In histopathological examination, aortic segments in the HC-diet rat revealed thickening intima and media, which were blocked by administration with MSC. Taken together, MSC could suppress the development of atherosclerosis in the HC-diet rat model through the inhibition of the aortic expression levels of pro-inflammatory and adhesion molecules.