• Journal of Species Research
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• v.3 no.1
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• pp.7-16
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• 2014

Many species of Lymantria are important forestry pests, including L. dispar which is well known distributed from Asia to North America as an invasive species. Like of most other genera of moths, the systematic of this genus is still in dispute, especially on the monophyly and the relationship within this genus due to the fact that genus is very large and varied. This genus was morphologically defined only by a single aphomorphy. To clarify the monophyly of the genus Lymantria, to reveal the phylogenetic relationship among the Indonesian species, and to establish the genetic characters of Indonesian Lymantria, we analyzed 9 species of Indonesian Lymantria involving 33 other species distributed around the world based on nucleotide sequence variation across a 516-bp region in the CO I gene. The results showed that the base composition of this region was a high A+T biased (C: 0.3333). The results also showed that the monophyly of Lymantria was not supported by bootstrap tests at any tree building methods. Indonesian species was distributed into four different groups but the relationship among them was still in dispute. It indicates that relationships among the basal nodes (groups) proposed here were least valid due to the fact that the number of species may not be enough to represent the real number of species in the nature. Moreover CO I gene sequences alone were not able to resolve their relationships at the basal nodes. More investigations were needed by including more species and other genes that the more conserved.

• Journal of Species Research
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• v.4 no.1
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• pp.49-56
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• 2015

Zeuzera is one of the most important wood borer pests in South East Asia. Like of most other genera of moths, the systematic of this genus is still in dispute, especially on the monophyly and the relationship within this genus due to the fact that genus is very varied. This genus was defined based on external characters only such as cross vein Sc-Rs present, humeral plate approximately triangular in shape and anal plate moderately long to moderately short. Therefore, the monophyly of this genus need to be evaluated based on more comprehensive data. To clarify the monophyly of the genus Zeuzera, to reveal the phylogenetic relationships among the Indonesian species, and to establish the genetic characters of Indonesian Zeuzera, we analyzed seven species of Indonesian Zeuzera including three other species distributed around the world based on nucleotide sequence variation across a 580-bp region in the CO I gene. The results showed that the monophyly of Zeuzera was supported by bootstrap tests at the MP and ML tree building methods (>95%). Genus Zeuzera was divided into two groups (A and B) with Z. borneana was excluded from the two groups and occupied at the basal node. Indonesian species was distributed into two different clades. CO I gene alone was able to fully resolve the relationships among species within clade B. However, further investigations were needed by including more species and other genes that the more conserved to test the validity of the phylogenetic hypothesis proposed here.

• Journal of Veterinary Science
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• v.22 no.6
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• pp.70.1-70.12
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• 2021

Bats are an important reservoir of several zoonotic diseases. However, the circulation of bat coronaviruses (BatCoV) in live animal markets in Indonesia has not been reported. Genetic characterization of BatCoV was performed by sequencing partial RdRp genes. Real-time polymerase chain reaction based on nucleocapsid protein (N) gene and Enzyme-linked immunosorbent assay against the N protein were conducted to detect the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral RNA and antibody, respectively. We identified the presence of BatCoV on Cynopterus brachyotis, Macroglossus minimus, and Rousettus amplexicaudatus. The results showed that the BatCoV included in this study are from an unclassified coronavirus group. Notably, SARS-CoV-2 viral RNA and antibodies were not detected in the sampled bats.

• Journal of Species Research
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• v.6 no.2
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• pp.181-189
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• 2017

Sixteen species of macrochelid mites were collected on the surface of scarab dung beetles in Mount Gede-Pangrango National Park, West Java, Indonesia. Of these, one species, Macrocheles gedeensis sp. nov. is described here as new to science. The other fifteen species were Holostaspella katakurai Hartini and Takaku, 2003, H. oblonga Hartini and Takaku, 2010, H. villosa Hartini and Takaku, 2010, Neopodocinum halimunense Hartini and Takaku, 2003, N. subjaspersi Hartini and Takaku, 2003, Neopodocinum sp., Macrocheles dispar (Berlese, 1910), M. entetiensis Hartini and Takaku, 2005, M. hallidayi Walter and Krantz, 1986, M. jabarensis Hartini and Takaku, 2003, M. kraepelini (Berlese, 1905), M. pumilus Hartini, Dwibadra and Takaku, 2009, M. turgoensis Hartini, Dwibadra and Takaku, 2009, M. sukabumiensis Hartini and Takaku, 2003, and M. sukaramiensis Takaku, 2001.

• Journal of Ecology and Environment
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• v.45 no.1
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• pp.24-39
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• 2021

Background: To address the lack of evidence supporting invasion by three invasive plant species (Imperata cylindrica, Lantana camara, and Chromolaena odorata) in tropical ecosystems, we compared the ecophysiological and leaf anatomical traits of these three invasive alien species with those of species native to Sempu Island, Indonesia. Data on four plant traits were obtained from the TRY Plant Trait Database, and leaf anatomical traits were measured using transverse leaf sections. Results: Two ecophysiological traits including specific leaf area (SLA) and seed dry weight showed significant association with plant invasion in the Sempu Island Nature Reserve. Invasive species showed higher SLA and lower seed dry weight than non-invasive species. Moreover, invasive species showed superior leaf anatomical traits including sclerenchymatous tissue thickness, vascular bundle area, chlorophyll content, and bundle sheath area. Principal component analysis (PCA) showed that leaf anatomical traits strongly influenced with cumulative variances (100% in grass and 88.92% in shrubs), where I. cylindrica and C. odorata outperformed non-invasive species in these traits. Conclusions: These data suggest that the traits studied are important for plant invasiveness since ecophysiological traits influence of light capture, plant growth, and reproduction while leaf anatomical traits affect herbivory, photosynthetic assimilate transport, and photosynthetic activity.

• Journal of the Korean Wood Science and Technology
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• v.40 no.4
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• pp.244-256
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• 2012

The anatomical characteristics of the ten Indonesian wood species (Gmelina, Jeunjing, Mangium, Durian, Gandaria, Jengkol, Kupa, Mangga, Nanaka and Rambutan) were investigated by optical microscopy. All the species were diffuse-porous with solitary and radial pore multiple. In Mangium, however, tangential pore multiple was observed as well. Tangential diameter of pore was larger than that of radial one except for Jeunjing and Nangka. Nangka showed the largest tangential diameter of pore among the species. Vessel number per $mm^2$ of Mangium, Gandaria and Kupa was higher than that of the other species. Especially Kupa showed highest vessel number. The tangential width of axial parenchyma cell in Gmelina, Mangium, Kupa and Mangga was larger than that of wood fiber, while the other species showed the opposite trend. Mangium was the largest in tangential width of axial parenchyma cell. Rays were homocellular composed only of procumbent cell in Gmelina, Jeunjing and Rambutan. Heterocellular rays composed of procumbent cells in the body and one row of upright and/or square in the margin are observed in Gandaria. Mangium, Durian, Jengkol, Kupa, Mangga and Nangkabody showed heterocellular rays composed of procumbent cells in the body and mostly 1~2 rows of upright and/or square cells in the margin. Crystals occurred in Durian, Gandaria, Jengkol, Jeunjing, Mangga and Rambutan and silica in Jeunjing and Mangga.

• Mycobiology
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• v.31 no.1
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• pp.19-22
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• 2003

The primary objective of the current research was to detect genetic variations within the Indonesian isolates of Cochliobolus heterostrophus collected from ecologically different places of the country at molecular level using PCR-RFLP analyses. The primer pair of NS3 and NS6 produced amplification fragment in all of the isolates tested. A single fragment of estimated 907 bp was observed in the PCR product pattern. RFLP analysis of the PCR product employing three restriction enzymes, HaeIII, HhaI, and RsaI, respectively, did not reveal intraspecific variations within the fungus. Similarly, nucleotide sequences of portion of small subunit of the ribosomal DNA gene of two of the isolates collected showed no appreciable differences, indicating the absence of genetic diversities among the isolates tested. A phylogenetic tree was constructed and the Indonesian C. heterostrophus, represented by SM-1 isolate, was found to be phylogenetically located near C. sativus, a closely related species.

• The Korean Journal of Ecology
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• v.23 no.2
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• pp.125-129
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• 2000

Gunung Gede Pangrango National Park is one of the Long-term Ecological Research Site in Indonesia. In the late 1997, the fires have burnt and destroyed nearly 300 ha forest in this park. and G. Masigit was the largest burnt area (250 ha) of nine locations of hot spot recognized. Undergrowth vegetation got the most severe impacts. Almost undergrowth vegetation in various location were totally burnt. However, within three months following burning new seedlings such as Omalanthus populneus, Macaranga, Trema orientalis and Eupatorium appeared in the forest floor- The number of mycoflora recorded in burnt forest was interestingly increased in post forest fires site. Forest fires in G. Masigit had also affected the wild life population and diversity. For example, the number of bird species and the number of soil insects in burnt forest was significantly reduced. The forest fires had also great impact on soil. such as on soil organic contents, bulk density, colour, consistency, permeability and the activity of soil microorganisms.

• Microbiology and Biotechnology Letters
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• v.47 no.3
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• pp.449-458
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• 2019

Hepatitis B treatments using immune therapy are gaining interest because of the improvements in dendritic cell performance for antigen presentation, which induces an appropriate immune response and raises patient survival rates. This research aims to produce a significant amount of the HBcAg antigen, which can induce an immune response and have a curative effect on HBV infection. In this study, the HBV subgenotype B3 of the HBcAg gene was used, which is dominant in Indonesia. Further, Lactococcus lactis bacteria was used as the host because of its safety and tightly regulated protein expression. The codon usage for the HBcAg gene was optimized to improve protein expression in L. lactis, which is important because a codon is not random between species. The HBcAg gene is attached to a pNZ8148 plasmid and transformed into the L. lactis NZ3900 expression host. The results confirm that a positive protein band (21 kDa) in two fractions of purified HBcAg was recognized by both western blotting and dot blot hybridization, even if the HBcAg optimized codon has higher GC contents than that suggested for L. lactis expression. Overall, this research strengthens the broad use of L. lactis bacteria for any protein expression, including higher protein expression of codon optimized HBcAg gene compared to non-optimized genes. Furthermore, the improvement in the codon optimization of the HBcAg gene significantly increases the total protein expression by 10-20%, and the expression level of the codon optimized HBcAg increases 1.5 to 3.2-times that of the native HBcAg.

• Journal of the Korean Wood Science and Technology
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• v.42 no.4
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• pp.467-476
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• 2014

The physical and mechanical characteristics of 10 Indonesian wood species were investigated. Mangium, Gandaria and Rambutan showed higher density. Mangium, Gandaria and Mangga appeared lower in shrinkage, and the ratio of tangential/radial was low in Albizia, Kupa and Mangga. The compression strength parallel to the grain and hardness were high in Mangium and Nangka. Gmelina, Mangium, Gandaria, Kupa, Nangka and Rambutan had valuable properties for commercial wood materials. Consequently, it is considered that the results of this study could be useful basic data for the improved use of planted and promising species in Indonesia.