• Mycobiology
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• v.46 no.3
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• pp.260-268
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• 2018

In an ongoing survey of Korean indigenous fungi, two fungal strains (KNU16-74 and KNU16-99) belonging to the genus Chrysosporium were isolated from field soil in Gyeongnam, Korea. Morphological characterization and phylogenetic analysis using sequence of the internal transcribed spacer regions were carried out to confirm its precise identification. These strains were identified as Chrysosporium indicum (KNU16-74) and Chrysosporium fluviale (KNU16-99). To examine the keratin degradation efficiency of these two fungal species, human hair strands were incubated with fungus culture. Results revealed that these two fungal species have the ability to degrade keratin substrate. This is the first report of these two species in Korea.

• Mycobiology
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• v.48 no.5
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• pp.423-426
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• 2020

Cypripedium japonicum is known to be the indigenous plant to Korea, Japan, and China. However, C. japonicum represents the most critically endangered plant species in South Korea. The plant is esthetically pleasing due to its flower, which is larger than any other orchidaceous species. Disease symptoms relating to gray mold were observed on C. japonicum in May 2019. The suspected pathogen was successfully isolated from the symptomatic leaf tissue and conducted a pure culture of the fungi. The conidia formed consisted of a colorless or light brown single cell, which was either egg or oval-shaped with a size of 7.1 to 13.4 × 5.2 to 8.6 ㎛. Molecular phylogenetic relationship analysis was also confirmed that the pathogen concerned belonging to the family of Botrytis cinerea. Therefore, the findings confirmed that the pathogen isolated from C. japonicum was consistent with the unique properties of B. cinerea.

• Journal of Forest and Environmental Science
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• v.40 no.1
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• pp.64-71
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• 2024

Mushroom is very important to rural dwellers due to the various economic, health and nutritional benefits that can be derived from its consumption, sales and utilization. Hence, this experiment was carried out with a view to evaluating the influence of locally available substrate i.e., sawdust of different wood species on the cultivation of Pleurotus ostreatus (oyster mushroom). Pleurotus ostreatus was grown on sawdust (indigenous and exotic species) using two pretreatment methods, which included hot water treatment to remove (a water-soluble extract) and non-extracted sawdusts (substrate). The result of this study showed that there was a significant difference in the effect of different wood species selected. Parkia biglobosa produced a better yield (71.06 g), followed by Anogeissus leiocarpus (53.17 g) and Gmelina arborea (51.39 g) in a non-treated unextracted states. In the treated samples, Parkia biglobosa has the highest yield (50.61 g) followed by Anogeissus leiocarpus (49.28 g) and Gmelina arborea (45.00 g). Thus, Parkia biglobosa and Anogeissus leiocarpus produced a higher yield of mushroom either in a treated or non-treated state and they could be recommended for growing oyster mushroom (Pleurotus ostreatus).

• Journal of Species Research
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• v.5 no.1
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• pp.166-178
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• 2016

An outcome of the study to discover indigenous prokaryotic species in Korea, a total of 26 bacterial species assigned to the classes Bacteroidetes and Firmicutes were isolated from diverse environmental samples collected from soil, tidal flat, freshwater, seawater, wetland, plant roots, and fermented foods. From the high 16S rRNA gene sequence similarity (>99.0%) and formation of a robust phylogenetic clade with the closest species, it was determined that each strain belonged to each independent and predefined bacterial species. There is no official report that these 26 species have been described in Korea; therefore 14 strains for the order Flavobacteriales and two strains for the order Cytophagales were assigned to the class Bacteroidetes, and 8 strains for the order Bacillales and 4 strains for the order Lactobacillales were assigned to the class Firmicutes are reported for new bacterial species found in Korea. Gram reaction, colony and cell morphology, basic biochemical characteristics, isolation source, and strain IDs are also described in the species description section.

• Journal of the Speleological Society of Korea
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• no.88
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• pp.7-25
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• 2008

In order to accumulate the basic informations to assess the scientific and natural values of the Hoe-ok Cave located in Mountain Chi-ak, Seong-nam 2 ri, sin-rim myeon, the distribution of the species in the cave were investigated from November 2004 to June 2005. The results are as follows. The species identified in the Hoe-ok Cave were 34 species, 28 family, 18 order, 8 classes. The dominant species were the 14 species(41.2%), 11 family, 7 order in the Class of Insecta. The ecological distribution of the Heo-ok Cave, 8 species(23.5％) of troglobite, 7 species of troglophiles(20.6％), 19 species of trogloxenes(55.9％) were found and the share of the species indigenous to the cave among the population was 44％, and the species which came out of the cave occupied the larger portion among the populations inside the whole cave.

• Journal of Microbiology and Biotechnology
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• v.20 no.1
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• pp.153-160
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• 2010

The present study was carried out for the isolation of bacteriocin-producing enterococci from indigenous sources. Gram-positive enterococci are known for having the ability to produce enterocins with good antimicrobial potential. A total of 34 strains were isolated from processed dairy products of Pakistan and seven out of them were found to be member of genus Enterococcus on selective enumeration. Biochemical and molecular characterization revealed that four of these isolates (IJ-03, IJ-07, IJ-11, and IJ-12) were Enterococcus faecalis and three (IJ-06, IJ-21, and IJ-31) were Enterococcus faecium. Local processed cheese was the source of all enterococcal isolates, except E. faecium IJ-21 and IJ-31, which were isolated from indigenous yoghurt and butter samples, respectively. Bacterial isolates were sensitive to commonly used antibiotics except methicillin and kanamycin. They also lacked critical virulence determinants, mainly cytolysin (cyl), gelatinase (gel), enterococcal surface protein (esp), and vancomycin resistance (vanA and vanB). Polymerase chain reaction amplification identified that enterocin A and P genes were present in the genome of E. faecium IJ-06 and IJ-21, whereas the E. faecium IJ-31 genome showed only enterocin P genes. No amplification was observed for genes that corresponded with the enterocins 31, AS-48, L50A, and L50B, and ent 1071A and 1071B. There were no signals of amplification found for E. faecalis IJ-11, indicating that the antimicrobial activity was because of an enterocin different from those checked by PCR. Hence, the indigenous bacterial isolates have great potential for bacteriocin production and they had antibacterial activity against a variety of closely related species.

• Journal of Microbiology and Biotechnology
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• v.23 no.8
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• pp.1123-1132
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• 2013

The removal of toxic Cr(VI) by microorganisms is a promising approach for Cr(VI) pollution remediation. In the present study, four indigenous bacteria, named LY1, LY2, LY6, and LY7, were isolated from Cr(VI)-contaminated soil. Among the four Cr(VI)-resistant isolates, strain LY6 displayed the highest Cr(VI)-removing ability, with 100 mg/l Cr(VI) being completely removed within 144 h. It could effectively remove Cr(VI) over a wide pH range from 5.5 to 9.5, with the optimal pH of 8.5. The amount of Cr(VI) removed increased with initial Cr(VI) concentration. Data from the time-course analysis of Cr(VI) removal by strain LY6 followed first-order kinetics. Based on the 16S rRNA gene sequence, strain LY6 was identified as Pseudochrobactrum asaccharolyticum, a species that had never been reported for Cr(VI) removal before. Transmission electron microscopy and energy dispersive X-ray spectroscopy analysis further confirmed that strain LY6 could accumulate chromium within the cell while conducting Cr(VI) removal. The results suggested that the indigenous bacterial strain LY6 would be a new candidate for potential application in Cr(VI) pollution bioremediation.

• Korean Journal of Environmental Agriculture
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• v.6 no.2
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• pp.66-72
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• 1987

Sensitivity of the three freshwater fish, Cyprinus carpio, Oryzias latipes (wildtype indigenous to Korea), and O. latipes (Japanese killifish) to 30 pesticide formulations were studied in terms of 48 hr $LC_{50}$ determined with the static method. The correlation between C. carpio and O. latipes (Japanese killifish) was higher than that between C. carpio and O. latipes with correlation coefficients of 0.89 and 0.80, respectively. The sensitivity of O. latipes and O. latipes (Japanese killifish) to pesticides showed very high correlation with a coefficient of 0.93. Therefore, it is suggested that the acute toxicity data concerning O. latipes (Japanese killifish) could represent those C. carpio or O. latipes which are indigenous species in Korea. Also, it is found that the present protocol for the toxicity test with carp in Korea has difficulties in maintaining the proper concentration of dissolved oxygen in the test chamber because of the abrupt decrease of dissolved oxygen to 2mg/l, which is not acceptable according to general guidelines of foreign countries.

• Journal of Animal Science and Technology
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• v.65 no.5
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• pp.912-921
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• 2023

Genetic diversity analysis is crucial for maintaining and managing genetic resources. Several studies have examined the genetic diversity of Korean domestic chicken (KDC) populations using microsatellite markers, but it is difficult to capture the characteristics of the whole genome in this manner. Hence, this study analyzed the genetic diversity of several KDC populations using high-density single nucleotide polymorphism (SNP) genotype data. We examined 935 birds from 21 KDC populations, including indigenous and adapted Korean native chicken (KNC), Hyunin and Jeju KDC, and Hanhyup commercial KDC populations. A total of 212,420 SNPs of 21 KDC populations were used for calculating genetic distances and fixation index, and for ADMIXTURE analysis. As a result of the analysis, the indigenous KNC groups were genetically closer and more fixed than the other groups. Furthermore, Hyunin and Jeju KDC were similar to the indigenous KNC. In comparison, adapted KNC and Hanhyup KDC populations derived from the same original species were genetically close to each other, but had different genetic structures from the others. In conclusion, this study suggests that continuous evaluation and management are required to prevent a loss of genetic diversity in each group. Basic genetic information is provided that can be used to improve breeds quickly by utilizing the various characteristics of native chickens.

• Korean Journal of Soil Science and Fertilizer
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• v.24 no.4
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• pp.293-301
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• 1991

Spores of the family Clomaceae, Acaulosporaceae. and Gigasporaceae in the ord er Clomales. isolated from greenhouse soils grown horticultural crop in the southern region of Korea, included those of the following species : Acaulospora biretculata Rothwell & Trappe, A. appendiculata Spain, Sievering & Schenck, A. foveata Trappe & Janos, A. denticulata Sievering and Toro. A. elegans Trappe & Gerd., A. rehmii Sieverding & Toro in Acaulospora species, Gigaspora gigantea (Nicol. & Gerd.) Cerd. & Trappe, G. decipiens Hall & Abbott in Gigaspora species, Glomus ambisporum Schenck & Smith, G. hoi Berch & Trappe, G. caledoniwn (Nicol. & Gerd.) Trappe & Gerd. in Glomus species, Scutellispora aurigloba (Hall) Walker & Sanders, S. calospora (Nicol. & Gerd.) Walker & Sanders, S. coralloidea (Trappe, Gerd. & Ho) Walker & Sanders in Scutellispora species. Sporocarps of Sclerocystis pachycaulis Wu & Chen were also found.