• 대한환경공학회지
• /
• 제32권2호
• /
• pp.121-130
• /
• 2010

산성광산배수(Acid Mine Drainage; AMD)는 낮은 pH조건에서 중금속 및 황산염이온 등이 다량 용존되어 환경오염 문제를 발생시킨다. 국내의 폐광산 일부에서는 산성광산배수를 처리하기 위해 정화시설이 운영되고 있으나 여전히 주변 하천에 영향을 미치고 있다. 본 연구는 산성광산배수 및 영향을 받는 하천에서 지표미생물의 특이적 유전자를 실시간 정량 중합효소 연쇄반응(Real-time quantitative Polymerase Chain Reaction; Real-time qPCR)을 이용하여 확인 및 정량함으로써 광산배수의 환경영향을 미생물학적으로 판단하고자 수행되었다. 지표 종으로 선정한 미생물은 16S rRNA 미생물 군집분석 결과 발견된 미생물 중 철환원균인 Rhodoferax ferrireducens T118, Acidiphilium cryptum JF-5이며 이 외에 기존에 광산에 존재하는 것으로 알려진 미생물 중 호산성 황환원균인 Desulfosporosinus orientus, 철산화균인 Leptosprillum ferrooxidans, 철 및 황산화균인 Acidothiobacillus ferrooxidans이었다. 최종적으로, 본 연구에서 각 광산의 광산배수가 하천에 미치는 영향을 정량적으로 판단하여 비교하기 위해 광산배수로 인한 하천에서의 미생물 변동 지수를 산정하였으며 연구 대상 4개 광산 중 광산배수 처리시설이 없는 삼탄의 광산배수의 경우 주변 방류 하천으로의 미생물학적 환경영향이 가장 큰것으로 나타났다

• 대한토목학회논문집
• /
• 제28권1B호
• /
• pp.153-160
• /
• 2008

농지-임야유역의 비점원으로부터 발생하는 미생물학적 오염물질 부하량을 추정하기 위하여, 농지와 임야가 혼합된 3개 시험유역에서 동일한 2개 강우사상에 대한 지표미생물 유출 특성을 조사하였으며, 지표미생물항목은 대장균군(total coliform: TC), 분원성 대장균(Fecal coliform: FC), 대장균 (Escherichia coli: EC), 분원성 연쇄상구균(Fecal streptococcus: FS)이었다. 농지-임야 유역의 강우시 유량변화에 따라 토사유실로 인하여 부유물질 농도가 상당히 증가하였다. 지표미생물 농도는 유량변화와 상당히 밀접한 관계를 보였다. 대부분 오염되지 않은 임야로 구성되어 있는 첫번째 유역의 강우유출수 TC EMC(Event Mean Concentration)는 $5.3{\times}10^3CFU/100ml$이었으며, FC EMC는$1.4{\times}10^3CFU/100ml$, EC EMC는 $1.1{\times}10^3CFU/100ml$, FS EMC는 $3.9{\times}10^2CFU/100ml$이었다. 임야유역과 농지유역이 혼합되어 있는 제 2 유역의 지표미생물에 대한 EMC는 TC EMC가 $1.7{\times}10^5CFU/100ml$, FC EMC가 $8.5{\times}10^4CFU/100ml$, EC EMC가 $8.9{\times}10^4CFU/100ml$, FS EMC가 $3.4{\times}10^4CFU/100ml$로 나타났다. 농지와 임야가 혼재되어 있으나, 유역면적이 큰 제 3 시험유역의 지표미생물에 대한 EMC는 TC EMC가 $1.9{\times}10^5CFU/100ml$, FC EMC가 $9.6{\times}10^4CFU/100ml$, EC EMC가 $7.0{\times}10^4CFU/100ml$, FS EMC가 $5.1{\times}10^4CFU/100ml$로 나타났다.

• Journal of Microbiology and Biotechnology
• /
• 제24권5호
• /
• pp.690-695
• /
• 2014

Bacterial 1-aminocyclopropane-1-carboxlyate (ACC) deaminase (AcdS) is an enzyme that cleaves ACC, a precursor of the plant hormone ethylene, into ${\alpha}$-ketobutyrate and ammonia. The acdS gene was cloned from Pseudomonas fluorescens, which was capable of improving the seedling of Chinese cabbage under salinity condition. The recombinant AcdS (rAcdS) exhibited optimal activity at pH 8.5 and $30^{\circ}C$. Strong activity was sustained at up to 100 mM NaCl. The polyclonal anti-P. fluorescens AcdS antibody was produced in a rabbit that had been immunized with the purified rAcdS. This antibody successfully recognized the homologous antigens derived from the total proteins of isolated plant growth-promoting microorganisms. A statistically significant correlation was observed between the intensity of hybridization signal and AcdS activity measured by a biochemical method, suggesting its application as a useful indicator for active deaminases.

• Environmental Engineering Research
• /
• 제14권1호
• /
• pp.63-67
• /
• 2009

Escherichia coli K-12 (E. coli K-12) is a representative indicator globally used for distinguishing and monitoring dynamic fates of pathogenic microorganisms in the environment. This study investigated how to most critically quantify lacZ ($\beta$-galactosidase) gene in E. coli K-12 by two different real-time polymerase chain reaction (real-time PCR) in association with three different DNA extraction practices. Three DNA extractions, i.e., sodium dodecyl sulfate (SDS)/proteinase K, magnetic beads and guanidium thiocyanate (GTC)/silica matrix were each compared for extracting total genomic DNA from E. coli K-12. Among them, GTC/silica matrix and magnetic beads beating similarly worked out to have the highest (22-23 ng/${\mu}L$) concentration of DNA extracted, but employing SDS/proteinase K had the lowest (10 ng/${\mu}L$) concentration of DNA retrieved. There were no significant differences in the quantification of the copy numbers of lacZ gene between SYBR Green I qPCR and QProbe-qPCR. However, SYBR Green I qPCR obtained somewhat higher copy number as $1{\times}10^8$ copies. It was decided that GTC/silica matrix extraction or magnetic beads beating in combination with SYBR Green I qPCR can be preferably applied for more effectively quantifying specific gene from a pure culture of microorganism.

• 한국식품영양학회지
• /
• 제10권3호
• /
• pp.414-421
• /
• 1997

먹거리의 국제화에 따라 식품이나 식품원료의 수입이 급증하고 식품규제에 대하여 국제적 조율이 강조되는 등 먹거리를 둘러싼 환경이 크게 변하고 있다. 식품산업도 고품질을 유지하면서 안전성을 확보하는 것이 시급해졌는데 안전성 확보에는 미생물학적, 물리적 위해(이물질 혼입 등)와 함께 식품첨가물, 잔류농약 등의 화학적 위해분석을 빠뜨릴 수 없다. 이들 먹거리의 안전성 평가기술의 대표적인 것이 HACCP이다. HACCP절차는 식품가공과 식품저장의 모든 형태에서 식품안전을 확인하는 필수요소이다. 전통적인 최종제품검사가 품질 결함을 찾아내기 위한 것이라면 HACCP은 식품의 안전성 결함을 방지하기 위하여 제품계통이나 가공인자 그리고 실제운전에 규제사항을 설계하는 것이다. 본보는 HACCP시스템에 필요한 기기를 중점적으로 요약한 것이다.

• 한국토양비료학회지
• /
• 제48권5호
• /
• pp.528-534
• /
• 2015

Green manure crops have been well recognized as the alternative for chemical fertilizer, especially N fertilizer, because of its positive effect on soil and the environment. Hairy vetch and green barley are the most popular crops for cultivation of rice in paddy field. This study was conducted to evaluate effects of hairy vetch and green barley on soil microbial community and chemical properties during short-term application (three years). For this study, treatments were composed of hairy vetch (Hv), green barley (Gb), hairy vetch + green barley (Hv+Gb), and chemical fertilizer without green manure crops (Con.). Hv+Gb treatment showed the highest microbial biomass among treatments. Principal component analysis (PCA) showed that PC1 (73.0 %) was affected by microbial biomass and PC2 (21.5 %) was affected by fungi, cy19:0/18:$1{\omega}7c$ (stress indicator). Combined treatment with hairy vetch and green barley could be more efficient than green manure crop treatment as well as chemical fertilizer treatment for improvement of soil microorganisms.

• Journal of Microbiology and Biotechnology
• /
• 제32권1호
• /
• pp.64-71
• /
• 2022

The discarding of wastes into the environment is a significant problem for many communities. Still, food waste can be used for lactic acid bacteria (LAB) growth. Here, we evaluated three growth media equivalent to de Mann Rogosa Sharpe (MRS), using apple bagasse, yeast waste, fish flour, forage oats, and cheese whey. Cell-free supernatants of eight LAB strains were tested for antimicrobial activity against nine indicator microorganisms. The supernatants were also evaluated for protein content, reducing sugars, pH, and lactic acid concentration. Cell-free supernatants from fish flour broth (FFB) LAB growth were the most effective. The strain Leuconostoc mesenteroides PIM5 presented the best activity in all media. L. mesenteroides CAL14 completely inhibited L. monocytogenes and strongly inhibited Bacillus cereus (91.1%). The strain L. mesenteroides PIM5 consumed more proteins (77.42%) and reducing sugars (56.08%) in FFB than in MRS broth (51.78% and 30.58%, respectively). Culture media formulated with agroindustrial wastes positively improved the antimicrobial activity of selected LAB, probably due to the production of antimicrobial peptides or bacteriocins.

• 한국지하수토양환경학회지:지하수토양환경
• /
• 제28권5호
• /
• pp.68-77
• /
• 2023

The effect of soil damage on the physicochemical characteristics and activity of the soil microbial community is not well known. This study investigates this relationship by analyzing 11 soil samples collected from various points of soil damage across Gyeonggi-do. Soil damage resulted from forest fires, landslides, and development areas, with their impacts most severe on the topsoil layer (0-30 cm). Dehydrogenase and β-glucosidase activities were notably higher at locations damaged by forest fires compared to other sites. While enzyme activities in soils influenced by landslides and development areas were relatively low, sites with a pollution history exhibited elevated dehydrogenase activity, likely due to past microbial response to the pollution. Additionally, an assessment of carbon substrate usability by soil microorganisms indicated higher substrate availability in areas impacted by forest fires, contrasting with lower availability in landslide and development sites. Statistical analysis revealed a positive correlation between organic content of sand and clay and microbial activity. These findings provide valuable insights into soil damage and associated restoration research, as well as management strategies.

• 센서학회지
• /
• 제29권2호
• /
• pp.93-99
• /
• 2020

A colorimetric paper sensor was used to detect volatile nitrogen-containing compounds emitted from spoiled salmon filets to determine their freshness. The sensing mechanism was based on acid-base reactions between acidic pH-indicating dyes and basic volatile ammonia and amines. A sensing layer was simply fabricated by drop-casting a dye solution of bromocresol green (BCG) on a polyvinylidene fluoride substrate, and its color-change response was enhanced by optimizing the amounts of additive chemicals, such as polyethylene glycol, p-toluene sulfonic acid, and graphene oxide in the dye solution. To avoid the adverse effects of water vapor, both faces of the sensing layer were enclosed by using a polyethylene terephthalate film and a gas-permeable microporous polytetrafluoroethylene sheet, respectively. When exposed to basic gas analytes, the paper-like sensor distinctly exhibited a color change from initially yellow, then to green, and finally to blue due to the deprotonation of BCG via the Brønsted acid-base reaction. The use of ammonia analyte as a test gas confirmed that the sensing performance of the optimized sensor was reversible and excellent (detection time of < 15 min, sensitive naked-eye detection at 0.25 ppm, good selectivity to common volatile organic gases, and good stability against thermal stress). Finally, the coloration intensity of the sensor was quantified as a function of the storage time of the salmon filet at 28℃ to evaluate its usefulness in monitoring of the food freshness with the measurement of the total viable count (TVC) of microorganisms in the food. The TVC value increased from 3.2 × 10⁵ to 3.1 × 10⁹ cfu/g in 28 h and then became stable, whereas the sensor response abruptly changed in the first 8 h and slightly increased thereafter. This result suggests that the colorimetric response could be used as an indicator for evaluating the degree of decay of salmon induced by microorganisms.

• 한국식품위생안전성학회지
• /
• 제26권4호
• /
• pp.289-295
• /
• 2011

본 연구는 상추를 대상으로 재배~수확 후 단계의 미생물학적 안전성을 평가하고자 이천지역 상추농가와 수확 후 처리시설에서 위생지표세균(일반세균수, 대장균군,E. coli)과 병원성미생물(Escherichia coli O157:H7, Salmonella spp., Stphylococcus aureus, Listeria monocytogenes, Bacillus cereus)을 조사하였다. 상추 생산과정의 미생물 오염도 조사 결과, 대부분의 시료에서 일반세균수 수준은 4.0 log CFU/(ml, g, $100\;cm^2$, hand)이었다. 또한 수확 후 처리시설에서 사용되는 장갑에서 E. coli가 3.5 log CFU/hand로 비교적 높게 검출되었고, B. cereus는 토양과 저울에서 각각 3.5 log CFU/g, 2.6 log CFU/$100\;cm^2$로 타시료에 비해 높았다. 특히 정식 후 10일 째 상추의 일반세균수, 대장균군수 및 B. cereus의 오염수준이 정식 후 62일째 상추 보다 각각 l.19, 1.87, and 1.94 log CFU/g 정도 높은 것으로 나타났다. 한편 E. coli O157:H7, Salmonella spp, 및 L. monocytogenes은 검출되지 않았다. 본 연구의 결과를 통하여 상추의 유해 미생물 오염은 생산단계부터 발생되고 있음이 확인되었고 이러한 유해미생물의 오염을 사전에 관리하기 위해서는 생산에서부터 소비까지 위해요소를 관리하는 시스템의 도입이 필요하다.