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Evaluation of the Effect of Mine Drainage on the Aquatic Environment by Quantitative Real-time PCR  

Han, Ji-Sun (Department of Environmental Engineering, Inha University)
Seo, Jang-Won (Department of Environmental Engineering, Inha University)
Ji, Won-Hyun (Mine Reclamation Corp.)
Park, Hyun-Sung (Mine Reclamation Corp.)
Kim, Chang-Gyun (Department of Environmental Engineering, Inha University)
Publication Information
Journal of Korean Society of Environmental Engineers / v.32, no.2, 2010 , pp. 121-130 More about this Journal
Abstract
Metals and sulfate can be considerably dissolved at low pH condition in the acid mine drainage(AMD) and it would make an environmental problems. There are only few of acid mine drainage treatment systems in Korea which are operating, but these still have an effect on the surrounding stream. In this study, quantification of indicator microorganisms was conducted to judge the environmental impact of AMD on microflora by quantitative real-time PCR in the drainage samples of four mines and the water samples of each surrounding stream. Two species of iron reducing bacteria(Rhodoferax ferrireducens T118 and Acidiphilium cryptum JF-5) were selected for indicator bacteria based on 16S rRNA cloning analysis, and sulfate reducing bacteria(Desulfosporosinus orientus), iron and sulfur oxidizing bacteria(Acidothiobacillus ferrooxidans) and iron oxidizing bacteria(Leptosprillum ferrooxidans) were included into indicator since these were found in the previous studies on the mining area. Thereafter, the comparative analysis of four mines were established by the microbiological variation index and it was determined that the biological environment effect of AMD is highest in Samtan mine which doesn t contain treatment system by the value.
Keywords
16S rDNA; real-time quantitative PCR; mine drainage; Molecular biological monitoring;
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