• Laboraroty Animal Research
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• v.34 no.4
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• pp.302-310
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• 2018

CD47 (integrin-associated protein), a multi-spanning transmembrane protein expressed in all cells including red blood cells (RBCs) and leukocytes, interacts with signal regulatory protein ${\alpha}$ ($SIRP{\alpha}$) on macrophages and thereby inhibits phagocytosis of RBCs. Recently, we generated a novel C57BL/6J CD47 knockout ($CD47^{-/-}$ hereafter) mouse line by employing a CRISPR/Cas9 system at Center for Mouse Models of Human Disease, and here report their hematological phenotypes. On monitoring their birth and development, $CD47^{-/-}$ mice were born viable with a natural male-to-female sex ratio and normally developed from birth through puberty to adulthood without noticeable changes in growth, food/water intake compared to their age and sex-matched wild-type littermates up to 26 weeks. Hematological analysis revealed a mild but significant reduction of RBC counts and hemoglobin in 16 week-old male $CD47^{-/-}$ mice which were aggravated at the age of 26 weeks with increased reticulocyte counts and mean corpuscular volume (MCV), suggesting hemolytic anemia. Interestingly, anemia in female $CD47^{-/-}$ mice became evident at 26 weeks, but splenomegaly was identified in both genders of $CD47^{-/-}$ mice from the age of 16 weeks, consistent with development of hemolytic anemia. Additionally, helper and cytotoxic T cell populations were considerably reduced in the spleen, but not in thymus, of $CD47^{-/-}$ mice, suggesting a crucial role of CD47 in proliferation of T cells. Collectively, these findings indicate that our $CD47^{-/-}$ mice have progressive hemolytic anemia and splenic depletion of mature T cell populations and therefore may be useful as an in vivo model to study the function of CD47.

• Economic and Environmental Geology
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• v.52 no.2
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• pp.119-128
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• 2019

About 300,000 tones of oyster shell are annually produced in Korea and, thus, a massive recycling plan is required. Many desulfurizing studies using oyster shells with chemical composition of $CaCO_3$ have been performed so far; however, most of them have focused on dry desulfurization. This study investigates the possibility of using oyster shells for wet desulfurization after calcination. For this, a simulated wet desulfurization facility of spray type was devised and compared the SOx-stripping characteristics of calcined oyster shell with those of limestone. The calcined oyster shell slurry indicate a better desulfurizability than the slurries of raw shell or limestone because the oyster shell transformed to a more reactive phase ($Ca(OH)_2$) by the calcination and hydration. Because of this reason, when the calcined oyster shell slurries were used, the reaction residue showed the higher gypsum ($CaSO_4{\cdot}2H_2O$) contents than any other cases. In the continuous desulfurization experiments, calcined oyster shell slurry showed a wider pH variation than limestone or raw oyster shell slurries, another clear indication of high reactivity of calcined oyster shells for $SO_2$ absorption. Our study also shows that the efficiency of wet desulfurization can be improved by the use of calcined oyster shells.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.1
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• pp.362-370
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• 2019

This study was conducted to investigate effects of 12 weeks of combined exercise training with blood flow restriction (BFR) on body composition (weight, %body fat, lean body mass, body mass index (BMI)) and cardiovascular responses (brachial-ankle pulse wave velocity (ba PWV) and ankle-brachial index (ABI)) in elderly women. Participants (N = 43, Females) were randomly assigned into a combined exercise with BFR (n = 14, BFR), only combined exercise (n =14, EX) or non-exercise control group (n = 15, CON). Two-way repeated measures ANOVA with contrast testing was utilized for data analysis. Alpha was set at p < 0.05. Body composition (weight, %body fat, BMI) in BFR was significantly changed, and %body fat in EX was significantly decreased, but there was no change in the CON. In addition, the right and left ba PWV values in the BFR were significantly decreased, while only the left side ba PWV in EX was significantly decreased and there was no change in the CON. Moreover, the % change and effect size of most variables in the BFR were higher than the EX. Taken together, the results indicate that even though BFR and EX groups performed the same combined exercise training, BFR had additional stimulations of the sympathetic nerve system due to blood flow restriction. Thus, BFR training is more beneficial and has greater effects on body composition and cardiovascular responses in elderly females.

• The Journal of the Petrological Society of Korea
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• v.27 no.4
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• pp.223-233
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• 2018

In this study, SHRIMP zircon U-Pb dating was performed on deformed granitic rocks in the Sancheong area in the northeastern part of the Jirisan area, southwest of Yeongnam Massif. Until now, these have been known as Precambrian or age-unknown old igneous rocks, but the U-Pb concordant ages obtained from two samples are $237.8{\pm}4.0Ma$ and $230.2{\pm}3.4Ma$, respectively, showing their emplacements in Early to Middle Triassic. These results indicate that the deformed granite was emplaced at about 238~230 Ma. The study area shows the characteristics of ductile deformation with prominent development of foliation, augen structure, and lineation. It is observed that the deformed granites occur as xenoliths within the syenite, indicating that the time of deformation is earlier than the intrusion of the syenite of about 220 Ma. The emplacement and deformation periods of the deformed granite is similar to that of Permo-Triassic granite gneisses distributed in the Gimcheon and Andong areas of the Yeongnam Massif. Taken together, the eastern part of the Yeongnam Massif, extending from the central part to the southwestern part, granite intrusions occurred at about 260-230 Ma, followed by metamorphism-deformation of about 230-220 Ma.

• Korean Journal of Family Social Work
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• no.55
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• pp.31-58
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• 2017

This study aims to explore the longitudinal reciprocal relationship between family conflict and depression level of household head. Using the Korean Welfare Panel study (KOWEPS) of 2006-2009, the study applied multiple regression analysis and autoregressive cross-lagged model to test the hypothesis. Results of multiple regression analysis indicate that single direction of the impact of family conflict on a head of household's levels of depression and the vise versa were statistically significant. That is, higher level of family conflict in 2006 caused an increased levels of depression of household head in 2009, controlling gender, age and depression level in 2006. Also, the higher level of depression of household head in 2006 increased the level of family conflict in 2009 fixed with same control variables. The autoregressive and cross-lagged coefficients of family conflict and a head of household's levels of depression were statistically significant during the 4 years. The findings support the family system theory, indicating that there are reciprocal causal relationships between the whole family conflict and individual depression level. The strategies of social welfare practice and policy should thus aim to decrease individual's levels of depression and improve positive family function simultaneously to break the vicious circle.

• 재활복지
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• v.21 no.2
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• pp.63-88
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• 2017

This study evaluated the actual status of special needs of the hearing-impaired person for disaster response. The analysis revealed a significant level of unmet needs in disaster response for hearing-impaired person. The 5 special needs in disaster response include: 1) communication needs, which involve securing the means to make an emergency rescue request and communicating information during the rescue process; 2) transportation needs, which indicate the effective evacuation capacity and the level of training; 3) medical needs, which address the degree of preparedness for physical and mental emergency measures and the delivery of health information for rescue and first aid process; 4) maintaining functional independence needs, which refer to the level of self-preparedness to minimize damage in disaster situations, and; 5) supervision needs, which correspond to a personalized support system provided to disaster-vulnerable groups.

• Microbiology and Biotechnology Letters
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• v.46 no.4
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• pp.326-333
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• 2018

In this study, we identified methods to improve heat stability and skin permeability of functional protein biopolymers, such as growth factors, enzymes, and peptides. The biopolymers participate in cellular activation and catalytic functions in vivo. Therefore, when applied to cosmetics, their efficacies are expected to be helpful for skin care. However, they have disadvantages that include instability to heat and low skin permeability due to their high molecular weight. To overcome these problems, we searched for a composition that increases heat stability. Stability was improved using a polymeric humectant having a long polyethylene glycol length, compared with a mono-molecular structure humectant. Next, to enhance skin permeation, a permeation enhancing peptide was selected from a phage library. The permeation enhancing peptide can be commonly used to promote the permeation of growth factors, enzymes, and peptides. Screening was performed on the polymeric humectant formulation. One dominant peptide from the modified-screening method was identified. Furthermore, it was confirmed that the permeability of the peptide was better than that of the peptide developed through a screening system based on phosphate-buffered saline. The data indicate that the polymeric humectant formulation will be helpful for increasing the heat stability of protein ingredients and that skin permeability could be increased by a formulation-specific, penetration-enhancing peptide.

• Journal of the Korean Association of Geographic Information Studies
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• v.22 no.1
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• pp.78-92
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• 2019

This study aims to evaluate the efficiency in the provision of employment services for the middle-aged by using spatial analysis in GIS. Based on location information of employment service institutions, we find service areas of the institutions and calculate regional rates of duplication and exclusion in terms of spatial coverage of the employment services. Taking into account potential demand for employment services, the regions with high priority in the provision of the services are identified. Among the regions, those with high exclusion rate of the services are designated as the regions of insufficient service level. Results indicate that Namyangju-si is a representative region of insufficient employment service level. To improve efficiency in the provision of employment services, we suggest to relocate employment service institutions that have been located in a region of high duplication rate such as Siheung-si, Danwon-gu, Gangnam-gu, Songpa-gu into the locality of Namyangju-si.

• Korean Journal of Clinical Laboratory Science
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• v.50 no.4
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• pp.399-405
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• 2018

The platelet count in clinical laboratories is essential for the diagnosis and treatment of hemostasis abnormalities, and accurate platelet counting in the low count range is of prime importance for deciding if a platelet transfusion is needed and for monitoring after chemotherapy. Quality control is designed to reduce and correct any deficiencies in the internal analytical process of a clinical laboratory prior to the release of patient results. Fragmented erythrocytes are the major confusing factors for platelet counting because of their similar size to platelets. The authors found that the low range QC values were out of 2SD with a Sysmex automatic analyzer in internal quality control process. Thus far, there has been little discussion on the relationship between hemolysis and the platelet parameters. Therefore, this study focused on the performance of automated platelet counts, including the PLT-F, the PLT-I, and PLT-O methods at the low platelet range using the low level QC materials and compared the 5 platelet parameters with the hemolyzed samples. The results showed that the CV was the smallest with PLT-F and P-LCR increased from 18.4 to 31.9% in the hemolysis samples. These results indicate that a more accurate estimation of the platelet counts can be achieved using the PLT-F method than the PLT-I method at the low platelet range. The use of the PLT-F system improves the confidence of results in low platelets samples in a routine hematology laboratory. The results suggest that P-LCR is a new parameter in assessing samples when the specimen is suspected of hemolysis and deterioration. Nevertheless, further studies will be needed to establish the relationship with P-LCR and hemolysis using human blood specimens.

• Korean Journal of Environmental Biology
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• v.37 no.1
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• pp.72-81
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• 2019

Bisphenol A (BPA), a representative endocrine disrupting chemicals, has adverse effects on growth, development and reproduction in aquatic organisms. The object of this study was to investigate the modulation of antioxidant enzyme-coding genes using quantitative real time RT-PCR (qRT-PCR), enzyme activity and total protein content, to understand oxidative stress responses after exposure to BPA for 48 h in brackish water flea Diaphanosoma celebensis. The BPA ($3mg\;L^{-1}$) significantly upregulated the expression of Cu/Zn-SOD, Mn-SOD, and catalase (CAT) mRNA. Three GST isoforms (GST-kappa, GST-mu, and GST-theta) mRNA levels significantly increased at the rate of $0.12mg\;L^{-1}$ of BPA. In particular, GST-mu showed the highest expression level, indicating its key role in antioxidant response to BPA. SOD activity was induced with a concentration-dependent manner, and total protein contents was reduced. These findings indicate that BPA can induce oxidative stress in this species, and these antioxidants may be involved in cellular protection against BPA exposure. This study will provide a better understanding of molecular mode of action of BPA toxicity in aquatic organisms.