• Title/Summary/Keyword: Incubating

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Formation of Acycloretinoic Acid by Autoxidation of Lycopene (Lycopene의 자동산화에 의한 Acycloretinoic acid의 생성)

  • Kim, Seon-Jae
    • Korean Journal of Food Science and Technology
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    • v.32 no.6
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    • pp.1437-1441
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    • 2000
  • Acycloretinoic acid was prepared from acycloretinal by oxidation with Tollens reagent. Acycloretinoic acid was separated with Silica-HPLC and analyzed by ODS-HPLC with a photodiode array detector and by GC-MS. Lycopene was solubilized in toluene and aqueous Tween 40, and then oxidized by incubating at $37^{\circ}C$ under atmospheric oxygen. Acidic compound was produced by autoxidation of lycopene. Retention time, UV-Vis spectra and mass spectra of the acidic compound were identical to the standard acycloretinoic acid. Thus, acycloretinoic acid was confirmed to occur in vitro under oxidation condition of lycopene.

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An Analysis of E-Commerce by Local Governments in Korea (한국 지방자치단체의 전자상거래 유형 분석)

  • Kim, Se Hun;Min, Daihwan
    • Journal of Information Technology Services
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    • v.14 no.4
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    • pp.31-44
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    • 2015
  • Local governments in Korea are involved in three types of online shopping sites : 'direct operation', 'outsourced operation', and 'sales agency.' They are different in terms of the site operator and the charging mechanism among a local government, the site operator, and local producers. This study analyzes their differences in site traffic, local producers' participation, promotion, and partnership. The results from the investigation of 72 shopping sites are the following. First, shopping sites of the 'direct operation' type and the 'outsourced operation' type show significantly higher participation of local producers. Second, the 'marketing agency' type has significantly higher number of promotional activities than the 'oursourced type' which has significantly higher number than the 'direct operation' type. Third, the number of partnerships in the 'marketing agency' type is the highest. The 'direct operation' type is the second and significantly higher than the 'outsourced type.' On the basis of the analysis results, this paper suggests an incubation ecosystem model as a better way of involvement by local governments in online shopping sites.

How can we Develop Students' Creativity? : Redesigning a Creativity Program based on Flow Theory

  • Park, JaeSung
    • Journal of Practical Engineering Education
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    • v.9 no.2
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    • pp.91-98
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    • 2017
  • To produce highly creative students, engineering education must develop both problem recognition skills and the ability to solve problems independently. Chonnam National University has been developing a creativity program in which students identify and solve problems themselves. Initially, the primary focus was on solving problems, but this was unsuccessful at attracting the students' interest. To overcome this, we adopted Csikszentmihalyi's flow theory in our creativity program, much like the way this theory is used in computer games. As a result of adopting flow theory, we achieved our goal. Thus, in this paper, we will introduce Chonnam National University's creativity program (designed based on flow theory), which we will call the Self-Directed Creativity-Upgrade Program. Furthermore, we will discuss the impact of this program based on statistical analyses.

A Study on the Design Direction of the Prefab Affordable Housing for the Urban Regeneration in Deteriorated Residential Area - Focused on the Gwang-Myeong 3-dong, Kunggi-do, South Korea - (노후주거지 재생을 위한 모듈러 지원주택 계획 연구 - 광명 3동 광이로 일대를 중심으로 -)

  • Yoo, Hae-Yeon;Hyun, Sun-Yung
    • Journal of the Architectural Institute of Korea Planning & Design
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    • v.35 no.11
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    • pp.35-42
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    • 2019
  • The purpose of this study was to study social housing and incubating module planning for the urban regeneration in the deteriorated residential areas. The site of this study is Gwange-ro Gwangmyeong 3-dong, where old residential areas are concentrated, and there are problems in the decreasing number of young people and the aging population and elderly population. In this study, the necessity of supporting housing according to local characteristics and various programs and facilities are proposed, and various alternatives of modular housing are derived. As a result, it proposed verification of local regeneration and activation and orientation of social housing and local regeneration programs using modularity in the future.

Factors Influencing Work-Related Use of Smartphones: An Empirical Investigation

  • JaeSung Park;HeeOck Rho;Joon Koh
    • Asia pacific journal of information systems
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    • v.28 no.3
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    • pp.204-219
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    • 2018
  • This paper touches the limitations of existing theories related to technology acceptance, which mainly focus on users' perceived ease of use and usefulness, and proposes the role for sensory capabilities and enjoyment in understanding smartphone use. Furthermore, we develop a model that explains smartphone usage and intention to use it for a task and analyzes 442 questionnaire survey responses of smartphone users. First, perceived usefulness, perceived sensory capability, and perceived enjoyment influenced smartphone usage. Second, we found that both perceived usefulness and smartphone use were significantly associated with users' intention to utilize the smartphone for their job-related tasks. Finally, both perceived sensory capability and enjoyment were found to be more powerful factors than ease of use in explaining smartphone use. From the study findings, implications and future research directions are also discussed.

An Empirical Study on Influence of Venture Preparation on Business Performance of Initial Venture Foundation: Focused on The Effect of Controlling The Period of Venture Preparation (창업준비성이 창업초기기업 경영성과에 미치는 영향에 관한 실증연구: 창업준비기간의 조절효과 중심)

  • Oh, jaiwoo;Lee, Donghyung;Kang, Jinkyu
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.10 no.4
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    • pp.67-80
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    • 2015
  • Although recent policies and regulations in Korea strongly advocate and encourage cultivation of venture foundations, studies on venture foundation and success are very limited and often primarily focus on entrepreneurship or individual quality as a venture founder in addition to such studies' validity in question. Therefore, this study primarily focuses on venture preparation process which is subject to venture founder's effort to verify the policy effectiveness in the relationship between venture preparation and business performance. Major goal of this study is to reduce social cost of venture failure by suggesting systematic policy support for venture foundations and analyzing the relationship between following variables: first, venture preparation and financial performance, second, venture preparation and non-financial performance, and third, venture preparation and business performance. 400 initial venture foundations less than 5 years are selected from KISED(Korea Institute Startup & Entrepreneurship Development) trend analysis to carry out statistical analysis using SPSS 18.0. To organize the data features, frequency analysis as well as descriptive statistics are performed to verify the hypothesis. As a result, sub-factors in measurement of venture preparation which are venture education period, venture benefit, and experience of incubating organization are selected as independent variables. Likewise, sub-factors in measurement of business performance which are financial performance and non-financial performance are used as dependent variables. To validate interactive effect, venture preparation period is selected as control variable to perform hierarchical regression analysis. The analysis result verifies that venture benefit has positive influence on financial and non-financial performance while venture education period has positive influence on non-financial influence and experience of incubating organization has negative influence on non-financial performance without influence on financial performance. In addition, interaction of venture preparation period has positive influence solely between venture benefit and non-financial performance. Through this study, appropriate supporting plans depending on the level of venture preparation can be derived to improve business performance of initial venture foundations for policy designer of venture support, and quality rather than quality improvement of venture businesses is possible through investigation of structural issues of individual venture businesses. Ultimately, this study suggests venture founders to determine whether to focus on venture preparation process or to start a venture business.

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In vitro Cytotoxicity Evaluation of Polydimethylsiloxane as a Biosensor Coating Material (바이오센서 코팅용 Polydimethylsiloxane의 생체외 세포독성 평가)

  • Park, Subeom;Lee, Jonghwan;Na, Kyunga;Jung, Jaeyeon;Kim, Myungjin;Park, Sungjae;Hyun, Jinho
    • Journal of Adhesion and Interface
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    • v.10 no.2
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    • pp.77-83
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    • 2009
  • PDMS was selected for a coating material of implantable biosensors and the cytotoxicity of extracts released from a polymer was evaluated using ISO 10993-5, Biological evaluation of medical devices-Part 5: Tests for in vitro cytotoxicity. Organo-tin was used as a positive control and a medium without serum was used as a negative control. Materials extract were prepared by incubating specimens in RPMI medium without serum ($125{\mu}L/cm^2$) for 24 h, 1 week and 6 weeks at $38^{\circ}C$. The evaluation of cytotoxicity was performed by two different methods : 1) seeding cells with extracts at the beginning 2) incubating extracts with cell sheets already formed on the plate. Both cell morphology and MTT numerical data were shown for the confirmation of cytotoxicity and cell spreading on the surface of PDMS.

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Comparison of Real-Time PCR and Conventional Culture Method for Detection of Cronobacter spp. in Powdered Foods (분말식품에서 Cronobacter spp. 검출을 위한 Real-Time PCR과 배지배양법의 비교검증)

  • Chon, Jung-Whan;Song, Kwang-Young;Kim, Sun-Young;Hyeon, Ji-Yeon;Kim, Yun-Gyeong;Hwang, In-Gyun;Kwak, Hyo-Sun;Seo, Kun-Ho
    • Korean Journal of Microbiology
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    • v.47 no.1
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    • pp.87-91
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    • 2011
  • The aim of this study was to compare the performance of conventional culture and real-time PCR for detection of Cronobacter spp. in powdered foods. Infant formula, baby food and Misugaru inoculated with Cronobacter were enriched in distilled water as first enrichment step, followed by incubating in Enterobacteriaceae enrichment (EE) broth as second enrichment step. A loopful of enriched sample was streaked onto Druggan-Forsythe-Iversen agar, followed by incubating at $37^{\circ}C$ for 24 h. One milliliter of the enriched distilled water and EE broth were used in real-time PCR assay. No statistical differences were observed in the number of positive samples between culture method and real-time PCR (p>0.05) in all types of food samples. The number of positives of real-time PCR was higher in the first enrichment media (distilled water) than the second enrichment media (EE broth), though there was no significant difference (p>0.05). It appears that some components of the second enrichment broth, EE broth, inhibit the reaction of real-time PCR. These results show that real-time PCR using a single enrichment with distilled water could be useful as an effective screening method for detection of Cronobacter while saving much time and labor compared to conventional culture method.

Effect of Temperature Condition on Nitrogen Mineralization and Soil Microbial Community Shift in Volcanic Ash Soil (온도가 화산회토양의 질소무기화와 미생물군집이동에 미치는 영향)

  • Joa, Jae-Ho;Moon, Doo-Gyung;Koh, Sang-Wook;Hyun, Hae-Nam
    • Korean Journal of Soil Science and Fertilizer
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    • v.45 no.4
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    • pp.467-474
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    • 2012
  • This study was carried out to evaluate effect of temperature condition on nitrogen mineralization of organic matter, distribution of microbial group by PLFA profiles, and soil microbial community structure in volcanic ash soil. Dried soil 30 g mixed well each 2 g of pellet (OFPE) organic fertilizers, pig manure compost (PMC), and food waste compost (FWC). And then had incubated at $10^{\circ}C$, $20^{\circ}C$, and $30^{\circ}C$, respectively. Nitrogen mineralization rate increased with increasing temperature and that was in the order of FWC>OFPE>PMC. Distribution ratio of microbial group by PLFA profiles were different significantly caused by incubation temperature and the type of organic matter. As incubating time passed, density of microbial group decreased gradually. The Gram-bacteria PLFA/Gram+ bacteria PLFA, Fungi PLFA/Bacteria PLFA, and Unsaturated PLFA/saturated PLFA ratios were decreased according to the increasing temperature gradually. But cy19:0/$18:1{\omega}7c$ ratio increased both FWC and PMC treatment. Principal component analysis using PLFA profiles showed that microbial community structure made up clearly at both 75 days ($10^{\circ}C$) and 270 days ($30^{\circ}C$) by temperature factor. As incubating time passed, microbial community structure shifted gradually.

Characteristics on the Incubation of Sulfur Compound-Oxidizing Strains Separated for the Removal of Malodor (악취제거를 위하여 분리한 황화합물 산화균주의 배양특성)

  • Lim, Dong Joon;Lim, Kwang-Hee
    • Korean Chemical Engineering Research
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    • v.47 no.6
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    • pp.788-794
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    • 2009
  • Both strains of KD-1212 and DAH-1056 were isolated and identified from animal manure-contaminated soil by screening bacterial strains for the removal of sulfur compound-malodor with such substrate as sodium thiosulfate or free sulfur. Then the characteristics on the incubation of these microbes were observed under various incubating-condition such as pH, temperature, aerobic or anaerobic, substrate(sulfur compound) concentration, nitrogen and carbon source and rotating speed for mixing, and the optimum incubating condition was established. The optimum pHs of KD-1212 and DAH-1056 were 7.0 and 4.0, respectively, and their optimum temperatures were in the range of $30{\sim}35^{\circ}C$. Another autotrophic strain, ED-1138, was isolated from contaminated soil. The strain DAH-1056 excelled a strain Thiobacillus sp. IW in eliminating hydrogen sulfide during the process of malodor-biofiltration with a fixed strain. The characteristics on the incubation of strain KD-1212 were observed under various substrate-concentrations, nitrogen and carbon sources. KD-1212 favored glucose and maltose, and yeast extract as carbon sources and nitrogen source, respectively. The optimum concentrations of substrate and nitrogen source were 25 mM of sodium thiosulfate and 0.5% yeast extract, respectively for the growth of strain KD-1212.