• 제목/요약/키워드: IncP

검색결과 1,363건 처리시간 0.041초

A Series of IncQ-Based Reporter Plasmids for Use in a Range of Gram-Negative Genera

  • O'Sullivan, Laura E.;Nickerson, Cheryl A.;Wilson, James W.
    • Journal of Microbiology and Biotechnology
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    • 제20권5호
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    • pp.871-874
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    • 2010
  • Many studies require expression analysis of the same gene/promoter across a range of bacterial genera. However, there is currently a lack of availability of reporters based on the broad-host-range IncQ replicon, which is compatible with a popular improved IncP transfer system that is self-transfer defective. We report IncQ lacZ reporter plasmids with features including (1) compatibility with IncP, IncW, and pBHR/pBBR replicons, (2) a variety of antibiotic markers (Sp-r, Sm-r, Km-r, Cm-r), (3) convenient mobilization via a novel self-transfer-defective IncP conjugation system, and (4) GenBank DNA sequences. Utility is demonstrated using three different promoters in different Gram-negative genera.

Low cost, printed P-OLED displays for entry into the flexible display market

  • MacKenzie, J.Devin;Breeden, J.J.;Carter, S.A.;Chen, J.P.;Hinkle, P.;Jones, E.;Kreger, M.A.;Nakazawa, Y.;Roeloffs, R.;Vo, Vung;Wilkinson, M.
    • 한국정보디스플레이학회:학술대회논문집
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    • 한국정보디스플레이학회 2006년도 6th International Meeting on Information Display
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    • pp.641-643
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    • 2006
  • Add-Vision has developed a low-cost print technology for P-OLED displays on flexible substrates that meets several essentials for a new technology including: (1) Functionality including low DC voltage and wide color gamut; (2) Utilization of inexpensive tools; (3) Performance matching entry applications and markets. AVI's process is based on large-area printing of a combination of doped emissive and air-stable cathode inks utilizing truly low-cost tools to create printed P-OLEDs.

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Construction of Conjugative Gene Transfer System Between E. coli and Moderately Thermophilic, Extremely Acidophilic Acidithiobacillus caldus MTH-04

  • Liu, Xianggmei;Lin, Jianqun;Zhang, Zheng;Bian, Jiang;Zhao, Qing;Liu, Ying;Lin, Jianqiang;Yan, Wangming
    • Journal of Microbiology and Biotechnology
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    • 제17권1호
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    • pp.162-167
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    • 2007
  • A genetic transfer system for introducing foreign genes to biomining microorganisms is urgently needed. Thus, a conjugative gene transfer system was investigated for a moderately thermophilic, extremely acidophilic biomining bacterium, Acidithiobacillus caldus MTH-04. The broad-hostrange IncP plasmids RP4 and R68.45 were transferred directly into A. caldus MTH-04 from Escherichia coli by conjugation at relatively high frequencies. Additionally the broad-hostrange IncQ plasmids pJRD215, pVLT33, and pVLT35 were also transferred into A. caldus MTH-04 with the help of plasmid RP4 or strains with plasmid RP4 integrated into their chromosome, such as E. coli SM10. The $Km^r\;and\;Sm^r$ selectable markers from these plasmids were successfully expressed in A. caldus MTH-04. Futhermore, the IncP and IncQ plasmids were transferred back into E. coli cells from A. caldus MTH-04, thereby confirming the initial transfer of these plasmids from E. coli to A. caldus MTH-04. All the IncP and IncQ plasmids studied were stable in A. caldus MTH-04. Consequently, this development of a conjugational system for A. caldus MTH-04 will greatly facilitate its genetic study.

Leadframe SiP with Conformal Shield

  • Kim, ByongJin;Sim, KiDong;Hong, SeoungJoon;Moon, DaeHo;Son, YongHo;Kang, DaeByoung;Khim, JinYoung;Yoon, JuHoon
    • 마이크로전자및패키징학회지
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    • 제23권4호
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    • pp.31-34
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    • 2016
  • System In Package (SiP) is getting popular and momentum for the recent wearable, IoT and connectivity application apart from mobile phone. This is driven by market demands of cost competitive, lighter and smaller/thinner and higher performance. As one of many semiconducting assembly products, Leadframe product has been widely used for low cost solution, light/ small and thin form factor. But It has not been applied for SiP although Leadframe product has many advantages in cost, size and reliability performance. SiP is mostly based on laminate substrate and technically difficult on Leadframe substrate because of a limitation in SMT performance. In this paper, Leadframe based SiP product has been evaluated about key technical challenges in SMT performance and electrical shield technology. Mostly Leadframe is considered not available to apply EMI shield because of tie-bar around package edge. In order to overcome two major challenges, connection bars were deployed properly for SMT pad to pad and additional back-side etching was implemented after molding process to achieve electrical isolation from outer shield coating. This product was confirmed assembly workability as well as reliability.

Pseudomonas cepacia의 전달성 TOL plasmid의 특성과 불화합성 (Characterization and Incompatibility of Transmissible TOL Plasmid from Pseudomonas cepacia)

  • 조병남;조인선;최순영;유재근;민경희
    • 미생물학회지
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    • 제27권4호
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    • pp.334-341
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    • 1989
  • Toluate 분해 플라스미드를 pseudomonase cepacia SUB37에서 분리하여 분자량은 한천 젤 전기영동으로 측정한 결과 79. (119kb)로 확인되었다. 이 TOL플라스미드는 Pseudomonas의 다른 균주와 다른 속의 균주에 전달되었다. m-toluate 분해에서 가장 중요한 역할을 하는 catechol-2,3-oxygenase 활성을 P. cepacia SUB37과 transconjugant의 조효소액으로부터 측정한 결과, P. putida mt 2에서와 같이, meta pathway를 거쳐 m-toluate를 분해하는 유전자들이 plasmid에 암호화됨을 알수 있었다. P. cepacia SUB37 유래의 새로운 TOL plasmid는 IncP-4 불화합성군에 속하였고, 이것은 아마도 P. putida의 IncP-9 그룹의 TOL 플라스미드의 유도체로 사료된다.

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한국인 부정교합자의 측두하악장애(TMD) 유병율과 그 기여요인에 관한 연구 (The Prevalence Study of TMD and the Associated Factors in Korean Malocclusion Patients)

  • 김명희;남동석
    • 대한치과교정학회지
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    • 제27권4호
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    • pp.523-538
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    • 1997
  • 서울대학교병원 치과 교정과에 내원한 6세 1개월에서 45세 8개월사이(평균 19세 6개월)의 부정교합자 205명(남 67명, 여 138명)을 대상으로 TMD 증상과 기여요인에 관한 설문조사와 Orthopantomogram, 경두개 방사선 사진(transcranial view)의 판독, 교정모형 검사 등을 시행하여 다음과 같은 결론을 얻었다. 1. Helkimo Anamnestic index는 Ai 0 $46.8\%$, Ai I $22.0\%$, Ai II $31.2\%$로 TND의 주관적 증상이 연령에 따라 증가하는 추세(p<0.001)를 보였으며 여성에서 높았다(p<0.05). 2. Orthopantomogram상 하악두의 이상소견은 flattening($4.4\%$)이 가장 많았으며 전체적으로 한가지 이상의 이상소견을 보인 경우는 $8.3\%$였다. 3. 목과 어깨의 동통(p<0.001) 이악물기, 입술을 깨무는 습관(이상 p<0.01). 두통(p<0.05)이 있는 경우 Ai가 높았다. 4. Angle II급에서 Orthopantomogram상 하악두 이상소견의 빈도가 높았고, 상악 대구치의 교모가 있을때 Ai가 높은 경향이 있었다(이상 p<0.05). 그외의 교합 요소는 TMD의 증상과 무관하였다. 5. 하악두 길이(Co'-Inc')와 하악지 길이(Co'-Go')가 짧고 하악두의 비율(Co'-Inc'/Co'-Go')이 낮을때, 그리고 좌우 하악지 길이 (Co'-Go'), 하악두 비율(Co'-Inc'/Co'-Go')이 다를때 Orthopantomogram상 하악두 이상소견이 많았다.

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