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http://dx.doi.org/10.4014/jmb.0912.12017

A Series of IncQ-Based Reporter Plasmids for Use in a Range of Gram-Negative Genera  

O'Sullivan, Laura E. (Department of Biology, Villanova University)
Nickerson, Cheryl A. (Biodesign Institute, Arizona State University)
Wilson, James W. (Department of Biology, Villanova University)
Publication Information
Journal of Microbiology and Biotechnology / v.20, no.5, 2010 , pp. 871-874 More about this Journal
Abstract
Many studies require expression analysis of the same gene/promoter across a range of bacterial genera. However, there is currently a lack of availability of reporters based on the broad-host-range IncQ replicon, which is compatible with a popular improved IncP transfer system that is self-transfer defective. We report IncQ lacZ reporter plasmids with features including (1) compatibility with IncP, IncW, and pBHR/pBBR replicons, (2) a variety of antibiotic markers (Sp-r, Sm-r, Km-r, Cm-r), (3) convenient mobilization via a novel self-transfer-defective IncP conjugation system, and (4) GenBank DNA sequences. Utility is demonstrated using three different promoters in different Gram-negative genera.
Keywords
IncQ plasmid; broad host range; IncP; pUZ8002; pQlacZ;
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