Journal of Microbiology and Biotechnology

  • 제20권5호
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  • Pages.871-874
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  • 2010
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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A Series of IncQ-Based Reporter Plasmids for Use in a Range of Gram-Negative Genera

  • O'Sullivan, Laura E. (Department of Biology, Villanova University) ;
  • Nickerson, Cheryl A. (Biodesign Institute, Arizona State University) ;
  • Wilson, James W. (Department of Biology, Villanova University)
  • 투고 : 2009.12.10
  • 심사 : 2010.02.04
  • 발행 : 2010.05.28
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초록

Many studies require expression analysis of the same gene/promoter across a range of bacterial genera. However, there is currently a lack of availability of reporters based on the broad-host-range IncQ replicon, which is compatible with a popular improved IncP transfer system that is self-transfer defective. We report IncQ lacZ reporter plasmids with features including (1) compatibility with IncP, IncW, and pBHR/pBBR replicons, (2) a variety of antibiotic markers (Sp-r, Sm-r, Km-r, Cm-r), (3) convenient mobilization via a novel self-transfer-defective IncP conjugation system, and (4) GenBank DNA sequences. Utility is demonstrated using three different promoters in different Gram-negative genera.

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참고문헌

  1. Choi, S. U., C. K. Lee, Y. I. Hwang, H. Kinoshita, and T. Nihira. 2004. Intergeneric conjugal transfer of plasmid DNA from Escherichia coli to Kitasatospora setae, a bafilomycin B1 producer. Arch. Microbiol. 181: 294-298. https://doi.org/10.1007/s00203-004-0654-8
  2. Curtiss, R., 3rd, S. Y. Wanda, B. M. Gunn, X. Zhang, S. A. Tinge, V. Ananthnarayan, H. Mo, S. Wang, and W. Kong. 2009. Salmonella enterica serovar Typhimurium strains with regulated delayed attenuation in vivo. Infect. Immun. 77: 1071-1082. https://doi.org/10.1128/IAI.00693-08
  3. de Lorenzo, V. 2009. Recombinant bacteria for environmental release: What went wrong and what we have learnt from it. Clin. Microbiol. Infect. 15 (Suppl 1): 63-65. https://doi.org/10.1111/j.1469-0691.2008.02683.x
  4. Gulig, P. A. and R. Curtiss, 3rd. 1987. Plasmid-associated virulence of Salmonella Typhimurium. Infect. Immun. 55: 2891-2901.
  5. Guzman, L. M., D. Belin, M. J. Carson, and J. Beckwith. 1995. Tight regulation, modulation, and high-level expression by vectors containing the arabinose pBAD promoter. J. Bacteriol. 177: 4121-4130. https://doi.org/10.1128/jb.177.14.4121-4130.1995
  6. Hou, Y. H., F. C. Li, S. J. Wang, S. Qin, and Q. F. Wang. 2008. Intergeneric conjugation in holomycin-producing marine Streptomyces sp. strain M095. Microbiol. Res. 163: 96-104. https://doi.org/10.1016/j.micres.2006.07.003
  7. Logan, B. E. and J. M. Regan. 2006. Electricity-producing bacterial communities in microbial fuel cells. Trends Microbiol. 14: 512-518. https://doi.org/10.1016/j.tim.2006.10.003
  8. McClelland, M., K. E. Sanderson, J. Spieth, S. W. Clifton, P. Latreille, L. Courtney, et al. 2001. Complete genome sequence of Salmonella enterica serovar Typhimurium LT2. Nature 413: 852-856. https://doi.org/10.1038/35101614
  9. Miller, J. 1992. A Short Course in Bacterial Genetics: A Laboratory Manual and Handbook for Esherichia coli and Related Bacteria. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
  10. Mukhopadhyay, A., A. M. Redding, B. J. Rutherford, and J. D. Keasling. 2008. Importance of systems biology in engineering microbes for biofuel production. Curr. Opin. Biotechnol. 19: 228-234. https://doi.org/10.1016/j.copbio.2008.05.003
  11. Parales, R. E. and C. S. Harwood. 1993. Construction and use of a new broad-host-range lacZ transcriptional fusion vector, pHRP309, for Gram- bacteria. Gene 133: 23-30. https://doi.org/10.1016/0378-1119(93)90220-W
  12. Patnaik, R. 2008. Engineering complex phenotypes in industrial strains. Biotechnol. Prog. 24: 38-47. https://doi.org/10.1021/bp0701214
  13. Purnick, P. E. and R. Weiss. 2009. The second wave of synthetic biology: From modules to systems. Nat. Rev. Mol. Cell Biol. 10: 410-422. https://doi.org/10.1038/nrm2698
  14. Rittmann, B. E. 2006. Microbial ecology to manage processes in environmental biotechnology. Trends Biotechnol. 24: 261-266. https://doi.org/10.1016/j.tibtech.2006.04.003
  15. Rosche, T. M., A. Siddique, M. H. Larsen, and D. H. Figurski. 2000. Incompatibility protein IncC and global regulator KorB interact in active partition of promiscuous plasmid RK2. J. Bacteriol. 182: 6014-6026. https://doi.org/10.1128/JB.182.21.6014-6026.2000
  16. Simons, R. W., F. Houman, and N. Kleckner. 1987. Improved single and multicopy lac-based cloning vectors for protein and operon fusions. Gene 53: 85-96. https://doi.org/10.1016/0378-1119(87)90095-3
  17. Sioud, S., B. Aigle, I. Karray-Rebai, S. Smaoui, S. Bejar, and L. Mellouli. 2009. Integrative gene cloning and expression system for Streptomyces sp. US 24 and Streptomyces sp. TN 58 bioactive molecule producing strains. J. Biomed. Biotechnol. 2009: 464986.
  18. Thomas, C. M. (ed.). 1989. Promiscuous Plasmids of Gram- Negative Bacteria. Academic Press, London.
  19. Thomas, C. M. and K. M. Nielsen. 2005. Mechanisms of, and barriers to, horizontal gene transfer between bacteria. Nat. Rev. Microbiol. 3: 711-721. https://doi.org/10.1038/nrmicro1234
  20. Warner, J. R., R. Patnaik, and R. T. Gill. 2009. Genomics enabled approaches in strain engineering. Curr. Opin. Microbiol. 12: 223-230. https://doi.org/10.1016/j.mib.2009.04.005
  21. Wiater, L. A., A. Marra, and H. A. Shuman. 1994. Escherichia coli F plasmid transfers to and replicates within Legionella pneumophila: An alternative to using an RP4-based system for gene delivery. Plasmid 32: 280-294. https://doi.org/10.1006/plas.1994.1067
  22. Wilson, J. W., C. Coleman, and C. A. Nickerson. 2007. Cloning and transfer of the Salmonella pathogenicity island 2 type III secretion system for studies of a range of Gram-negative genera. Appl. Environ. Microbiol. 73: 5911-5918. https://doi.org/10.1128/AEM.00952-07
  23. Wilson, J. W. and C. A. Nickerson. 2006. A new experimental approach for studying bacterial genomic island evolution identifies island genes with bacterial host-specific expression patterns. BMC Evol. Biol. 6: 2. https://doi.org/10.1186/1471-2148-6-2
  24. Wilson, J. W., E. A. Sia, and D. H. Figurski. 1997. The kilE locus of promiscuous IncP alpha plasmid RK2 is required for stable maintenance in Pseudomonas aeruginosa. J. Bacteriol. 179: 2339-2347. https://doi.org/10.1128/jb.179.7.2339-2347.1997
  25. Wood, T. K. 2008. Molecular approaches in bioremediation. Curr. Opin. Biotechnol. 19: 572-578. https://doi.org/10.1016/j.copbio.2008.10.003

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