• Title/Summary/Keyword: Inbred Lines

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Effect of Apical Ear Removal on Characters of Subapical Ear in Corn Inbreds (옥수수 백식계통의 상부이삭 제거가 하부이삭의 형질에 미치는 영향)

  • Lee, Myoung-Hoon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.40 no.3
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    • pp.334-339
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    • 1995
  • Removal of apical ear after silking is a method to increase seed production in corn inbreds. Due to the phenomenon of apical dominance, the subapical ear would develope and produce seeds following the removal of the apical ear. This experiment was conducted to investigate the varietal difference in subapical ear development and seed production. Days to silking of subapical ears were delayed by about 4 days compared to normal apical ears. Cob length and filled ear length of subapical ears across seven inbreds tested were reduced by 21% and 36%, respectively. Those of inbred INV302 decreased less and subapical ear of inbred NC246 were barren, which incicated the varietal defferences. Number of kernel rows, kernels per row, and total kernels per ear also decreased. The mean reduction rates for those characters were 28%, 37%, and 47%, respectively. Weights of ears and kernels were reduced and showed different response among inbred lines. Coefficients of variations for cob and filled ear lengths, number of kernel rows and kernels were greater in subapical ears than in apical ears. Seed production of subapical ears was 40 ~ 80% of apical ears, depending on inbred lines when the apical ear is removed immediately after silking.

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Characteristics of Waesungri Maize (Zea mays L.) Inbred with Multi Tillers and Ears for Crude Forage Use

  • Lee, Hee-Bong
    • Plant Resources
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    • v.4 no.1
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    • pp.31-35
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    • 2001
  • Major characteristics of new Waesungri maize inbred line has multi-tiller and ears: five to six tillers and seven to eight ears per plant and flowering date of Waesungri was delayed about 18 and 24 days compared to Mo17 U.S line and IK$_4$ Korean local lines, respectively. Number of ears, fresh and dry weight per plant were significantly different among all tested hybrids including Waesungri/Sinkihong hybrid under different planting times and densities. Especially, both fresh and dry weight of IK$_1$/FR140//Waesungri F$_1$hybrid were significantly higher at high planting density. In kernel weight per unit area, Wnesungri/Sinkihong hybrid was high at high density and IK$_1$/FR140//Waesungri hybrid was high at low planting density. As results of analysis of variance, flowering date was shown a significantly different both planting times and varieties, while other characters including stem height were shown very variable in interactions with enviromental factors.

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Characteristics of Early Growth in Inbred Line of Dangyang Waxy Maize ("단양찰" 자식계통의 초기생육 특성)

  • Ji, Hee Chung;Kim, Choong-Soo;Lee, Hee-Bong
    • Korean Journal of Agricultural Science
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    • v.33 no.2
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    • pp.149-157
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    • 2006
  • The objective of this research was to know useful genetic characteristics for breeding program of corn. These materials were inbreds after selfing of six or eight generations and had useful genetic informations. The emergence of Danyang Waxy Corn was faster than those of other inbred lines at 2~3 days in field conditions. The plant height of Dangyang Waxy Corn was the highest at 8 days after emergence. However the plant height of New Dangjin was the shortest with 6.25cm at 8 days after emergence, the fresh weight of New Dangjin was 0.046g at 2 days after emergence but that of Dangyang was the heaviest with 0.180g. The fresh weight of 9 inbred lines had more increments in 2 days after emergence. The mean values of dry weight also showed similar trends in 9 inbred lines. The shoot dry weight of inbred lines, Dangyang and New Dangjin was 0.045g and 0.018g at 8 days after emergence, respectively. The root length of inbred line, Dangyang, was the longest with 64.4cm at 8 days after emergence. But the root length of New Dangjin was the shortest with 20.4cm at 8 days after emergence. The fresh weight of endosperm was 0.35g at 2 days after emergence and 0.26g at 8 days after emergence in Dangyang Waxy Corn, because of reduced nutrition of endosperm.

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Diallel Crosses Block Designs for Control versus Test Inbred Lines Comparisons

  • Son, Young-Nam;Choi, Kuey-Chung
    • Journal of the Korean Data and Information Science Society
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    • v.13 no.2
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    • pp.175-184
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    • 2002
  • In this paper, diallel crosses block designs for control versus test comparisons among the lines are proposed. These block designs are constructed by using partially balanced incomplete block designs with C-properties. Also, the efficiencies of the diallel crosses block designs obtained through this method are tabulated for number of lines 22 or less.

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Characterization of Androgenic and Gynogenic Doubled Haploids from Inbred Cultivars (연초의 약배양 및 종간교배에 의한 반수체 배가계통의 특성)

  • 금완수;이승철;김달웅
    • Journal of the Korean Society of Tobacco Science
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    • v.10 no.1
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    • pp.21-26
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    • 1988
  • Ten anther-derived doubled haploid (ADH) and 10 maternally-derived doubled haploid fines (MDH) from NC 2326 were evaluated in the field to compare their performance. Both anther-derived doubled haploid and maternally-derived doubled haploid lines were agronomically inferior to selfed parental variety for many parameters, but the degree of inferiority of the MDH lines were not significantly different from the selfed parental variety for any character investigated. The inferiority of the ADH lines were, however, significantly different from the selfed parental variety for yield, leaf length and width , and days to flower.

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Block Designs for Diallel Crosses with Different Group Characteristics

  • Kim, Seo-Young;Bae, Jong-Sung;Han, Wean-Sik
    • Communications for Statistical Applications and Methods
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    • v.9 no.1
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    • pp.53-61
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    • 2002
  • In this paper we proposed the method of the block designs for diallel crosses when inbred lines are divided into two groups. These block designs are derived by using kornecker product designs with two balanced incomplete block designs, and their efficiency factor evaluated. A table of diallel crosses for up to p$_1$$\leq$15 lines, p$_2$$\leq$15 lines in each set is also provided.

Breeding of inbred lines 'Wongyo 3115' and 'Wongyo 3116' in Strawberry (딸기 근교계통 '원교3115호'와 '원교 3116호' 육성)

  • Jeong, Ho Jeong;Lee, Sun Hee;Cho, Il whan;Rho, Il Rae
    • Horticultural Science & Technology
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    • v.33 no.3
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    • pp.443-447
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    • 2015
  • Fragaria ${\times}$ ananassa (Duch.) cultivars, 'Wongyo 3 115' a nd 'Wongyo 3 116', we re bre d as a n inte rme diate parent l ine using heterosis of $F_1$ hybrids in octoploid strawberry. These two new cultivars were developed as inbred lines ($S_9$ generation) propagated from both seed and runners/stolons. 'Wongyo 3115', an inbred line derived from the 'Benihoppe' cultivar, has a semi-upright plant shape showing a vigorless growth habit, weaker than the original 'Benihoppe' cultivar; it has early flower bud differentiation and produces 10 flowers per cluster when grown from healthy seed. 'Wongyo 3115' has low yields of conical type fruit with pink colored skin; average fruit weight is approximately 11 g. The important characteristic of 'Wongyo 3115' is its excellent fruit firmness, firmer than the original variety. 'Wongyo 3116', an inbred line derived from the 'Doyonoka' cultivar, has an upright plant shape, vigorless growth habit, weaker than that of the original 'Doyonoka' cultivar; it has early flower bud differentiation and produces 8 flowers per cluster when grown from healthy seed. 'Wongyo 3 116' has oblate type fruits with red skin and an ave rage weight of approximately 12 g. The important characteristics of 'Wongyo 3116' are excellent fruit shape and higher yield than other inbred lines, although it produces small fruits compared to the original cultivar. Both cultivars have excellent taste, high sugar/acid ratio, and good texture. In regards to disease and pest resistance, 'Wongyo 3115' and 'Wongyo 3116' have a tendency to be sensitive to powdery mildew, anthracnose and the two-spotted spider mite.

Identification of a Single Nucleotide Polymorphism (SNP) Marker for the Detection of Enhanced Honey Production in Hoenybee (수밀력 우수 꿀벌 계통 판별을 위한 계통 특이 분자마커 개발)

  • Kim, Hye-Kyung;Lee, Myeong-Lyeol;Lee, Man-Young;Choi, Yong-Soo;Kim, Dongwon;Kang, Ah Rang
    • Journal of Apiculture
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    • v.32 no.3
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    • pp.147-154
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    • 2017
  • Honeybees (Apis mellifera) are common pollinators and important insects studied in agriculture, ecology and basic research. Recently, RDA (Rural Development Administration) and YIRI (Yecheon-gun Industrial Insect Research Institute) have been breeding a triple crossbred honey bee named Jangwon, which have the ability to produce superior quality honey. In this study, we identified a single nucleotide polymorphism (SNP) marker in the genome of Jangwon honeybee, particularly, in the paternal line (D line). Initially, we performed Sequence-Based Genotyping (SBG) using the Illumina Hiseq 2500 in 5 honeybee inbred lines; A, C, D, E, and F; and obtained 1,029 SNPs. Seventeen SNPs for each inbred line were generated and selected after further filtering of the SNP dataset. The 17 SNP markers validated by performing TaqMan probe-based real-time PCR and genotyping analysis was conducted. Genotyping analysis of the 5 honeybee inbred lines and one hybrid line, $D{\times}F$, revealed that one set of SNP marker, AmD9, precisely discriminated the inbred line D from the others. Our results suggest that the identified SNP marker, AmD9, is successful in distinguishing the inbred honeybee lines D, and can be directly used for genotyping and breeding applications.

Evaluation of Germplasm and Development of SSR Markers for Marker-assisted Backcross in Tomato (분자마커 이용 여교잡 육종을 위한 토마토 유전자원 평가 및 SSR 마커 개발)

  • Hwang, Ji-Hyun;Kim, Hyuk-Jun;Chae, Young;Choi, Hak-Soon;Kim, Myung-Kwon;Park, Young-Hoon
    • Horticultural Science & Technology
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    • v.30 no.5
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    • pp.557-567
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    • 2012
  • This study was conducted to achieve basal information for the development of tomato cultivars with disease resistances through marker-assisted backcross (MAB). Ten inbred lines with TYLCV, late blight, bacterial wilt, or powdery mildew resistance and four adapted inbred lines with superior horticultural traits were collected, which can be useful as the donor parents and recurrent parents in MAB, respectively. Inbred lines collected were evaluated by molecular markers and bioassay for confirming their disease resistances. To develop DNA markers for selecting recurrent parent genome (background selection) in MAB, a total of 108 simple sequence repeat (SSR) primer sets (nine per chromosome at average) were selected from the tomato reference genetic maps posted on SOL Genomics Network. Genetic similarity and relationships among the inbred lines were assessed using a total of 303 polymorphic SSR markers. Similarity coefficient ranged from 0.33 to 0.80; the highest similarity coefficient (0.80) was found between bacterial wilt-resistant donor lines '10BA333' and '10BA424', and the lowest (0.33) between a late blight resistant-wild species L3708 (S. pimpinelliforium L.) and '10BA424'. UPGMA analysis grouped the inbred lines into three clusters based on the similarity coefficient 0.58. Most of the donor lines of the same resistance were closely related, indicating the possibility that these lines were developed using a common resistance source. Parent combinations (donor parent ${\times}$ recurrent parent) showing appropriate levels of genetic distance and SSR marker polymorphism for MAB were selected based on the dendrogram. These combinations included 'TYR1' ${\times}$ 'RPL1' for TYLCV, '10BA333' or '10BA424' ${\times}$ 'RPL2' for bacterial wilt, and 'KNU12' ${\times}$ 'AV107-4' or 'RPL2' for powdery mildew. For late blight, the wild species resistant line 'L3708' was distantly related to all recurrent parental lines, and a suitable parent combination for MAB was 'L3708' ${\times}$ 'AV107-4', which showed a similarity coefficient of 0.41 and 45 polymorphic SSR markers.

An Improved Plant Regeneration Protocol using Cotyledonary Explants from Inbred Lines of Chinese Cabbage (Brassica rapa ssp. pekinensis)

  • Yang Zhi Hong;Jin Hua;Plaha Prikshit;Woong Bae Tae;Jiang Guo Bin;Woo Jong Gyu;Yun Han Dae;Lim Yong Pyo;Lee Hyo Yeon
    • Journal of Plant Biotechnology
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    • v.6 no.4
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    • pp.235-239
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    • 2004
  • We studied the effect of genotype, explant, age of explant, medium (plant growth regulators and gelling agents), and standardized an efficient regeneration protocol for inbred lines of Chinese cabbage (Brassica rapa ssp. pekinensis). Of the different concentrations of NAA and BA tested, the combination of $5\;\cal{mg/L}\;BA\;and\;0.5\;\cal{mg/L}$ NAA gave the highest frequency of shoot regeneration. The cotyledonary explants had more shoot regeneration frequency ($\ge40\%$) than the hypocotyl ex-plants. Besides, the cotyledonary explants, excised from the 4-day old seedlings, showed higher shoot regene-ration ($56.7\%$). Of the three gelling agents and their concentrations used, 16 g/L agar was found to be the best for shoot regeneration. Shoot regeneration frequency in-creased significantly by supplementing the medium with $4\;\cal{mg/L}\;of\;AgNO_3$ MS medium devoid of NAA showed higher frequency of rooting in the regenerated shoots than the ones supplemented with NAA. Our improved regeneration protocol will be especially useful for the genetic transformation of Chinese cabbage inbred lines to develop transgenic hybrids.