Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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An Improved Plant Regeneration Protocol using Cotyledonary Explants from Inbred Lines of Chinese Cabbage (Brassica rapa ssp. pekinensis)

  • Yang Zhi Hong (Genome Research Center, Department of Horticulture, Chungnam National University) ;
  • Jin Hua (Genome Research Center, Department of Horticulture, Chungnam National University, Institute of Biological Resources and Environmental Research, Da Lian Nationalties University) ;
  • Plaha Prikshit (Genome Research Center, Department of Horticulture, Chungnam National University, Advanced Centre of Hill Bioresources & Biotechnology, HP Agricultural University) ;
  • Woong Bae Tae (Department of Horticulture, College of Agriculture and Life Science, Cheju National University) ;
  • Jiang Guo Bin (Institute of Biological Resources and Environmental Research, Da Lian Nationalties University) ;
  • Woo Jong Gyu (National Horticultural Research Institute, RDA) ;
  • Yun Han Dae (Division of Applied Life Science, Gyeongsang National University) ;
  • Lim Yong Pyo (Genome Research Center, Department of Horticulture, Chungnam National University) ;
  • Lee Hyo Yeon (Department of Horticulture, College of Agriculture and Life Science, Cheju National University)
  • Published : 2004.12.01
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Abstract

We studied the effect of genotype, explant, age of explant, medium (plant growth regulators and gelling agents), and standardized an efficient regeneration protocol for inbred lines of Chinese cabbage (Brassica rapa ssp. pekinensis). Of the different concentrations of NAA and BA tested, the combination of $5\;\cal{mg/L}\;BA\;and\;0.5\;\cal{mg/L}$ NAA gave the highest frequency of shoot regeneration. The cotyledonary explants had more shoot regeneration frequency ($\ge40\%$) than the hypocotyl ex-plants. Besides, the cotyledonary explants, excised from the 4-day old seedlings, showed higher shoot regene-ration ($56.7\%$). Of the three gelling agents and their concentrations used, 16 g/L agar was found to be the best for shoot regeneration. Shoot regeneration frequency in-creased significantly by supplementing the medium with $4\;\cal{mg/L}\;of\;AgNO_3$ MS medium devoid of NAA showed higher frequency of rooting in the regenerated shoots than the ones supplemented with NAA. Our improved regeneration protocol will be especially useful for the genetic transformation of Chinese cabbage inbred lines to develop transgenic hybrids.

Keywords

References

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