• Journal of Ginseng Research
• /
• 제33권1호
• /
• pp.65-71
• /
• 2009

This experiment was done to determine the optimum conditions for the induction of tetraploidy in Panax ginseng C. A. Meyer using bud length, temperature and plant growth regulator pretreatments. Highest callus formation was obtained when the medium was inoculated with flower bud in the size of 2-3 mm in length. The optimum temperature for the callus formation was high when treated at $4^{\circ}C$ for 4-5 days. Among the treatments of growth regulators and different concentration, highest callus formation was observed in combination of 5 mg/L 2,4-D and 1 mg/L kinetin for P. ginseng. As a result of flow cytometer analysis, all 7 adventitious roots were confirmed as tetraploidys. Cytological analysis revealed that the chromosome number of tetraploid roots was 96, while that of diploid roots was 48. Tetraploid ginseng roots were inoculated to flower bud size of 2-3 mm in length. The callus formation was optimum when treated with 1 mg/L 2,4-D at $4^{\circ}C$ for 5 days. Compared with control roots, tetraploid roots were thicker and longer and had few lateral branches. Fresh weight of tetraploid roots was relatively higher than the control roots.

• 한국자원식물학회지
• /
• 제22권4호
• /
• pp.281-286
• /
• 2009

홍경천의 잎, 줄기, 뿌리 절편을 이용하여 기내 부정근의 생산 체계를 확립하였다. 먼저 홍경천의 잎, 줄기, 뿌리 절편을 0.1, 1.0, 3.0 및 5,0 mg/L의 IBA와 sucrose가 10, 30, 50 및 100 g/L가 첨가된 MS 배지위에 치상하여 부정근의 유도율을 조사하였다. 부정근의 유도는 잎, 줄기 절편에서 IBA의 농도가 5.0 mg/L 일때 가장 높은 유도율을 보였으며, 뿌리 절편은 IBA 3.0 mg/L 첨가된 배지에서 부정근 유도율이 가장 높았다. Sucrose의 농도는 30 g/L가 첨가되었을 때 잎, 줄기, 뿌리 절편에서 높은 유도율을 나타났다. 고체배지 조건에서 부정근의 유도율이 가장 우수한 조건을 기본으로 액체배양을 실시하였으며, 염의 농도에 따른 부정근의 증식조건을 조사하였다. 1/3MS 배지에서 홍경천의 부정근을 배양하였을 때 1/2MS, MS 액체 배지조건 보다 약 2배, 2.5배의 부정근 생장량을 보였다.

• Journal of Plant Biotechnology
• /
• 제39권3호
• /
• pp.205-211
• /
• 2012

Pueraria lobata is a perennial legume plant, widely distributed in the countries of East Asia. It is a medicinally important leguminous plant and produces various isoflavones such as puerarin, daidzein etc which have potential for preventing several chronic diseases including osteoporosis, cardiovascular disease and cancer. In this study, we tried to induce hairy roots in vitro from Korean wild arrowroot P. lobata and investigated the effects of hormones and light conditions. Initially leaf and stem segments were infected with Agrobacterium rhizogenes and incubated in different conditions. Hairy roots were induced from only stem segments and the induction was best at dark condition and the presence of IBA during incubation. Secondary roots were also significantly much more induced at the dark condition than at the 16 hours light condition. Among plant growth regulators of auxin, IBA was best for secondary root formation while 2,4-D, IAA and NAA produced callus or less hairy roots. The presence of the foreign gene rolC transferred by A. rhizogenes that plays a major role in hairy root induction was confirmed by PCR. The accumulation of isoflavones such as puerarin and daidzin was also confirmed. These results will facilitate mass production of hairy root and can be used for the production of functional substances from wild arrowroots.

• Applied Biological Chemistry
• /
• 제41권8호
• /
• pp.600-604
• /
• 1998

Methanol extracts from 53 species of oriental medicinal plants in 34 families were tested for their fungicidal activities against Pyricularia grisea, Rhizoctonia solani, Phytophthora capsici, Phytophthora infestans, Collectotrichum dematium, Botryospaeria dothidea, Fusarium oxysporum f. sp. cucumerinum, Botrytis cinerea, Puccinia recondita, and Erysiphe graminis. In in vitro study using impregnated paper disc method, the efficacy varied with both plant pathogen and plant species tested. Methanol extracts of Asarum sieboldii roots, Sinomenium acutum roots, Pinus densiflora leaves, Rheum undulatum root barks, Coptis japonica roots, and Phellodendron amurense barks showed potent fungicidal activities against the various pathogens when treated with 10 mg/disc. In a whole plant test, methanol extracts of P. densiflora leaves and roots and C. japonica roots were highly effective against a variety of plant pathogens. As a naturally occurring fungicide, P. densiflora- and C. japonica-derived materials could be useful as new fungicidal products against various plant diseases induced by plant pathogenic fungi.

• Journal of Plant Biotechnology
• /
• 제4권1호
• /
• pp.33-37
• /
• 2002

Micropropagation and adventitious root production via the culture of Polygonatum odoratum were performed. Stem segments of seedlings of Polygonatum odoratum were the most efficient explants for adventitious shoot formation compared to leaf and root segments. Exogenous cytokinin treatment was required for adventitious shoot formation. Among the cytokinin (BA, Kinetin and Zeatin) tested, BA was most effective for shoot formation from stem segments. Auxin (NAA or IBA) in combination with cytokinin significantly enhanced adventitious shoot formation. Twenty five percent of explants produced adventitious shoots on medium with 2.0 mg/L BAP alone, while 83% of explants produced adventitious shoots on medium with the combination of 2.0 mg/L BAP and 0.1 mg/L IBA. Rooting of adventitious shoots was achieved after transferring to 112 MS medium supplemented with 0.1 mg/L IBA and 0.5 mg/L zeatin. When stem segments were cultured on MS medium with various kinds of auxin (IBA, NAA and 2,4-D), adventitious roots were formed from callus. frequency of adventitious root formation was highest in 2,4-D than NAA and IBA. When roots were in clusters together with parental stem segments, growth of roots actively occurred in hormone-free MS liquid medium. The above results represent that possible application for the mass production of roots and plantlets through in vitro culture system of Polygonatum odoratum.

• Natural Product Sciences
• /
• 제16권4호
• /
• pp.280-284
• /
• 2010

During liver fibrosis, hepatic stellate cells (HSCs) undergo a complex activation process characterized by increased proliferation and extracellular matrix deposition, which is the major pathological feature of hepatic cirrhosis. Therefore, suppression of HSCs activation has been proposed as therapeutic strategies for hepatic fibrosis. We tried to screen the antifibrotic activity of natural products employing HSC-T6, hepatic stellate cell lines as an in vitro assay system. In the present study, we investigated the antiproliferative activity of aerial parts and roots of Pulsatilla koreana Nakai (Ranunculaceae). Our present study shows that roots of P. koreana exerted more strong inhibitory activity compared to its aerial parts. In addition, among the fractions of the aqueous methanolic extract of P. koreana roots, both n-hexane and $CHCl_3$ fraction showed the strong inhibitory activity on HSC proliferation. Further study also demonstrated that the n-hexane and $CHCl_3$ fraction of P. koreana roots significantly inhibited the HSC proliferation in time- and concentration-related manners.

• 한국자원식물학회지
• /
• 제22권5호
• /
• pp.454-460
• /
• 2009

본 실험은 여우구슬의 기내 부정근 유도 및 증식조건의 확립을 목적으로 수행되었다. 우선 여우구슬의 기내 발아체로부터 부위를 달리하여 부정근을 유도한 결과 줄기부위는 뿌리보다 양호한 부정근의 유도를 보였다. 또한 유도된 부정근을 이용하여 옥신의 종류(IAA, IBA, NAA와 2.4-D)에 따른 부정근 유도율을 조사한 결과 IBA와 NAA는 IAA와 2.4-D보다 높은 유도율을 보였다. IBA의 농도에 따른 유도율과 증식효율은 IBA가 0.5 mg/L첨가되었을 때 가장 높은 유도 및 증식효율을 보였다. 최적의 액체배지조건을 확인하고자 IBA의 농도는 0.5 mg/L로 첨가하고 sucrose의 농도를 달리하여 실험한 결과 sucrose는 30 g/L 첨가 되었을 때 가장 높은 생중량과 건중량을 나타냈다. 액체배양된 여우구슬의 부정근을 각각 MS, 1/2MS, 1/3MS배지에 30 g/L sucrose, 0.5 mg/L IBA가 첨가된 5 L 용량의 생물반응기에 4주간 배양한 결과 1/2MS 배지에서 양호한 생장을 보였다. 본 실험에서는 여우구슬의 종자발아체를 이용하여 부정근의 유도 및 증식조건에 필요한 기내배양조건과 2차적으로 유도된 부정근을 이용하여 플라스크와 생물반응기 배양을 통한 효율적인 증식조건을 확립하였다.

• 한국약용작물학회지
• /
• 제23권5호
• /
• pp.357-362
• /
• 2015

Background : A series of studies were conducted to optimize adventitious root induction in vitro from explants of Astragalus membranaceus using various nutrient media supplemented with plant hormones. Methods and Results : Levels of active components were analyzed from adventitious roots induced under different media conditions. Among the different media conditions, Murashige and Skoog medium supplemented with $1.0mg{\cdot}{\ell}^{-1}$ indole-3-butyric acid resulted in the greatest adventitious root induction rate. The amount of the major active component of the adventitious roots of Ama1, calycosin-7-O-${\beta}$-D-glucopyranoside was higher than that of other adventitious root samples. Conclusions : These results suggest that the adventitious roots of A. membranaceus could be used for the commercial production of medicines.

• Plant Resources
• /
• 제7권3호
• /
• pp.222-225
• /
• 2004

In Japan, propagation of cyclamen is mainly from seedlings. However, seeds are expensive and germination is slow and non..uniform. Therefore, to achieve genetically uniform propagation, multiplication must be vegetative. The rooted tuberous stems without shoots as sources of explants were cultured on the media containing BA and sucrose. After 30 days cultivation, tuberous roots were produced from the root ends attached to a tuberous stem and its capability was dependent on the type of media. The highest percentage of tuberous root formation was observed in Culture on the medium of 1/3 MS containing 0.05mgL$^{-1}$ NAA, 0.5mg L$^{-1}$ BA and 5% sucrose. Growth rates of the tuberous roots were greatly influenced by the cutting positions of a root in explants. The highest growth of was observed if small amount of root end was cut at initiation of tissue culture.

• 한국식품영양과학회지
• /
• 제24권6호
• /
• pp.1010-1015
• /
• 1995

Extent of reduction in protein digestibility by Godulbaegi(lxeris sonchifolia H.) kimchi powder appeared to be related to the kimchi weight-to-protein weight ratio, the kind of protein sources, part of plants and fementation period. In vitro digestibilities of protein were significantly(p<0.05) reduced as the freeze dried kimchi powder weight-to-protein ration increased from 0.5 : 1.0 to 1.0 : 1.0 for all Godulbaegi samples and protein sources. Overall digestibility of protein sources in the presence of kimchi powder, the reduction ranked in the following order : soybean>casein>beef>squid. Lower(p<0.005) reduction occurred for each protein source when raw plant was exchanged for kimchi products. Some greater reduction of digestibility was noted in young plants and leaf samples than ripe or root samples. Trypsin inhibitor, which expressed as soybena trypsin inhibitor, was inversely related(r=0.8437) to in vitro protein digestibility of casein in the presence of Godulbaegi kimchi powder. More than three times of total polyphenols contained in leaves than in roots. Young leaves had 30% more total polyphenols(37.64mg/g sample) than that in ripe ones. Soaking in 5% NaCl solution for 24 hrs was markedly reduced in total polyphenols as 73% for leaves and 33% for roots. Remarkable reduction in total polyphenols was not checked during fermentation followed after soaking. Trypsin inhibitor content correlated well(r=0.8873) with total polyphenols in all of Godulbaegi samples.