Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Production of Adventitious Root and Analysis of Effective Components from in vitro Culture of Astragalus membranaceus

기내배양을 통한 황기 부정근의 생산과 유효성분 분석

  • Hur, Mok (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Lee, Dae Young (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Lee, Jae Won (Department of Herbal Crop Research, NIHHS, RDA) ;
  • An, Tae Jin (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Lee, Jeong Hoon (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Kim, Young Guk (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Cha, Seon Woo (Department of Herbal Crop Research, NIHHS, RDA) ;
  • Um, Yurry (Department of Herbal Crop Research, NIHHS, RDA)
  • 허목 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 이대영 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 이재원 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 안태진 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 이정훈 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 김영국 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 차선우 (농촌진흥청 국립원예특작과학원 인삼특작부) ;
  • 엄유리 (농촌진흥청 국립원예특작과학원 인삼특작부)
  • Received : 2015.08.12
  • Accepted : 2015.09.18
  • Published : 2015.10.30
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Abstract

Background : A series of studies were conducted to optimize adventitious root induction in vitro from explants of Astragalus membranaceus using various nutrient media supplemented with plant hormones. Methods and Results : Levels of active components were analyzed from adventitious roots induced under different media conditions. Among the different media conditions, Murashige and Skoog medium supplemented with $1.0mg{\cdot}{\ell}^{-1}$ indole-3-butyric acid resulted in the greatest adventitious root induction rate. The amount of the major active component of the adventitious roots of Ama1, calycosin-7-O-${\beta}$-D-glucopyranoside was higher than that of other adventitious root samples. Conclusions : These results suggest that the adventitious roots of A. membranaceus could be used for the commercial production of medicines.

Keywords

References

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