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In vitro culture of adventitious root from Rhodiola sachalinensis  

Bae, Kee-Hwa (Division of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University)
Yoon, Eui-Soo (Department of Biological Science, Kongju National University)
Choi, Yong-Eui (Division of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University)
Publication Information
Korean Journal of Plant Resources / v.22, no.4, 2009 , pp. 281-286 More about this Journal
Abstract
Rhodiola sachalinensis is one of the most popular oriental medicines in East Asia. It is a perennial herb, belonging to the family Crassulaceae, which is mainly distributed in mountains at the altitudes of 1700-2500 m in Baek-Du mountain. Cultivation of this species for the production of medicine materials is not easy in nature, because of restrict habitats. In vitro production of roots can be applicable for the production of medicinal materials. Here, we investigated the optimal conditions for induction and proliferation of adventitious roots in in vitro culture system. Leaf, stem and root segments from R. sachalinensis were cultured on Murashige and Skoog(MS) medium supplemented with the various concentrations of IBA(Indole-3-butyric acid)(0.5, 1.0, 3.0, and 5.0 mg/L) and sucrose(10, 30, 50, and 100 g/L). The optimal explant for adventitious root induction was leaf segment. Induction of adventitious roots was highest on MS medium supplemented with 5.0 mg/L IBA and sucrose 30 g/L. In liquid culture, fresh weight of adventitious roots was highest(15.65 g) on 1/3 strength MS liquid medium supplemented with 5.0 mg/L IBA with 30 g/L sucrose. These results revealed the first attempt for the production of adventitious roots in R. sachalinensis.
Keywords
IBA(Indole-3-butyric acid); Leaf; Root; Stem; Sucrose;
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1 Kim, J.A., X.L. You, C.H. Ahn, J.S. Lee and Y.E. Choi. 2007. Plant regeneration via direct adventitious roots from free root segments of Ulmus davidiana Planch. J. Kor. Forest Soc. 96: 83-88.(in Korean)
2 Linch, P.T., Y.H. Kim, S.P. Hong, J.J. Jian and J.S. Kang. 2000. Quantitative determanation of salidroside and tyrosol from the chromatography. Arch. Pharm. Res. 23:349-352   DOI   ScienceOn
3 Ming, H.Q., G.C. Xia and R.D. Zhang. 1988. Advanced research on Rhodiola. Chin. Trad. Herbal. Drugs. 19:229-234
4 Oh, J.K., O.Y. Shin, H.J. Jung and E.J. Lee. 2006. Effect of Rhodiola sachalinensis administration and endurance exercise on insulin sensitivity and expression of proteins related with glucose transport in skeletal musele of obese zucker rat. The J. of Kor. Nutrition Soc. 39:323-330(in Korean)
5 Yu, K.W., Hahn E.J. and K.Y. Paek. 2000. Production of adventitious ginseng roots using bioreactor. Kor. J. Plant. Tiss. Cult. 27:309-315.(in Korean)   과학기술학회마을
6 Xu, J.F., Z.G. Su and P.S. Feng. 1998b. Suspension culture of compact callus aggregate of Rhodiola sachalinensis for improved salidroside production. Enzym. and Micro. Tech. 23:20-27   DOI   ScienceOn
7 Zhong, Y., K. Lowell, J.A. Ping, C.T. Che, J.M. Pezzuto and H.H. Fong. 1991. Phenolic constituents of Rhodiola coccinea a tibetian folk medicine. Planta Med. 57:589   DOI   ScienceOn
8 Li, H.B. and F. Chen. 2001. Preparative isolation and purification of salidroside from the Chinese medicinal plant Rhodiola sachalinensis by high-speed counter-current chromatography. J. Chroma. 932:91-95   DOI   ScienceOn
9 Shimomura, K., H. Sudo, H. Saga and H. Kamada. 1991. Shikonin production and secretion by hairy root cultures of Lithospermum erythrorhizon. Plant Cell Rep. 10:282-285   DOI   ScienceOn
10 Choi, D.Y., S.Y. Ahn, S.G. Lee, J.S. Han, E.C. Kim, H.B. Lee, J.H. Shin, E.K. Kim and K.H. Row. 2004. Separation and performance test of whitening agent in Rhodiola sachalinensis. Kor. J. Biotech. Bioeng. 3:169-173.(in Korean)
11 Xu, J.F., Z.G. Su and P.S. Feng. 1998a. Activity of tyrosol glucosyltransferase and improved salidroside production through biotransformation of tyrosol in Rhodiola sachalinensis cell culture. J. Biotech. 61:69-73   DOI   ScienceOn
12 Bae, K.H., S. Lim, E.S. Yoon, C.G. Shin, Y.Y. Kim and Y.S. Kim. 2005. Effect of cytokinin and putrescine on plant regeneration from leaf explant of Rhodiola sachalinensis A. Bor. Kor. J. Plant Biotech. 32:195-199.(in Korean)   과학기술학회마을   DOI
13 Jung, S.J., J.H. Jeong, E.S. Yoon and Y.E. Choi. 2007. Plant regeneration from callus and adventitious root segments of Pulsatilla koreana Nakai. Kor. J. Plant Biotech. 34:153-159   과학기술학회마을   DOI   ScienceOn
14 Ahn, J.H. and S.Y. Lee. 2004. Effects of growth regulators on callus induction and plant regeneration from leaf explants of Sedium sarmentation. Kor. J. Plant Biotech. 31:25-29.(in Korean)   DOI
15 Chang, S.W., I.H. Kim and T.J. Han. 1999. Anthraquinone productivity by the cultures of adventitious roots and hairy roots from Cureled dock(Rumex crispus). Kor. J. Plant Tiss. Cult. 26:7-14   과학기술학회마을
16 Wu, S.X., Y.G. Zu and M. Wu. 2003. High yield production of salidroside in suspension culture of Rhodiola sachalinensis. J. Biotech. 106:33-43   DOI   ScienceOn
17 Jiang, M., W. Zhong and H. Han. 1994. Studies on producing effective medicinal ingredients of Rhodiola sachalinensis by tissue culture. Chin. J. Shen. Univ. 25:355-359
18 Yoon., E.S. 1997. Effect of plant growth regulators on plant regeneration from leaf and stem explant culture of Sedium erythrostichum Miq. Kor. J. Plant Biotech. 24:285-289.(in Korean)   과학기술학회마을
19 Toivonen, L. and Rosenqvist H. 1995. Establishment and growth characteristics of Glycyrrhiza glabra hairy root culture. Plant Cell Tissue Org Culture 41: 249-258   DOI   ScienceOn
20 Yan, XF, S.X. Wu, Y. Wang, X.H. Shang and S.J. Dai. 2004. Soil nutrient factors related to salidroside production of Rhodiola sachalinensis distributed in Chang Bai Mountain. Env. Exp. Bot. 53:267-276   DOI   ScienceOn
21 Petkov, V.D., D. Yonkov, A. Mosharoff, T. Kambourova, L. Alova, V. Petkov and I. Todorov. 1986. Effects of alcohol aqueous extract from Rhodiola rose L. root on learning and memory. Act. Physiol. Pharmacol. Bulg. 12:3-16
22 Kim, S.J., B. Hwang, S.J. Hwang and J.C. Ahn. 2004. Production of salidroside from callus culture of Rhodiola sachalinensis A. Bor. Kor. J. Plant Biotech. 1: 89-94.(in Korean)   과학기술학회마을
23 Lee, M.W., Y.H. Lee, H.M. Park, S.H. Tosh, E.J. Lee, H.D. Jang and Y.H. Kim. 2000. Antioxidant phenolic compounds from the roots of Rhodiola sachalinensis A. Bor. Arch. Pharm. Res. 23:455-458   DOI   ScienceOn
24 Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473-479   DOI