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http://dx.doi.org/10.7783/KJMCS.2015.23.5.357

Production of Adventitious Root and Analysis of Effective Components from in vitro Culture of Astragalus membranaceus  

Hur, Mok (Department of Herbal Crop Research, NIHHS, RDA)
Lee, Dae Young (Department of Herbal Crop Research, NIHHS, RDA)
Lee, Jae Won (Department of Herbal Crop Research, NIHHS, RDA)
An, Tae Jin (Department of Herbal Crop Research, NIHHS, RDA)
Lee, Jeong Hoon (Department of Herbal Crop Research, NIHHS, RDA)
Kim, Young Guk (Department of Herbal Crop Research, NIHHS, RDA)
Cha, Seon Woo (Department of Herbal Crop Research, NIHHS, RDA)
Um, Yurry (Department of Herbal Crop Research, NIHHS, RDA)
Publication Information
Korean Journal of Medicinal Crop Science / v.23, no.5, 2015 , pp. 357-362 More about this Journal
Abstract
Background : A series of studies were conducted to optimize adventitious root induction in vitro from explants of Astragalus membranaceus using various nutrient media supplemented with plant hormones. Methods and Results : Levels of active components were analyzed from adventitious roots induced under different media conditions. Among the different media conditions, Murashige and Skoog medium supplemented with $1.0mg{\cdot}{\ell}^{-1}$ indole-3-butyric acid resulted in the greatest adventitious root induction rate. The amount of the major active component of the adventitious roots of Ama1, calycosin-7-O-${\beta}$-D-glucopyranoside was higher than that of other adventitious root samples. Conclusions : These results suggest that the adventitious roots of A. membranaceus could be used for the commercial production of medicines.
Keywords
Astragalus membranaceus; Adventitious Roots; Calycosin-7-O-${\beta}$-D-Glucopyranoside;
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Times Cited By KSCI : 6  (Citation Analysis)
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