• Journal of Microbiology and Biotechnology
• /
• v.11 no.3
• /
• pp.491-496
• /
• 2001

A modification of decarboxylating agar medium as described by niven was performed to improve the detection method of biogenic amine-producing bacteria and to eliminate the false-positive. A total of 120 bacterial strains isolated from kimchi were used to evaluate different dicarboxylating agar media and for screening biogenic amines. Potential false-positives ranged from approximately 66 to 79% of the strains tested in the already well-known media. In our improved medium, none of the 120 strains showed the potential false-positives. There was a good agreement (81.7%-87.5%) between the results obtained by the improved medium and by HPLC analysis. Consequently, this medium was greatly improved in screening biogenic amine-producing bacteria and discarding false-positives. Of the 120 kimchi isolates, 14.2, 18.3, 37.5, and 0.8% were found by HPLC to be the producers of histamine, tyramine, putrescine (as a form of spermine), and cadaverine, respectively. The proportion of biogenic amine producer during kimchi fermentation increased to a maximum at an immature period and decreased thereafter.

• Korean Journal of Food Science and Technology
• /
• v.53 no.6
• /
• pp.815-818
• /
• 2021

The objective of this study was to develop a differential medium with improved selectivity for the isolation of Bacillus cereus. Mannitol egg yolk polymyxin medium supplemented with D-galactose allowed the differentiation of diarrheal- and emetic-type B. cereus through pH monitoring. The pH of the medium decreased significantly when incubating the emetic-type B. cereus, whereas the pH change was not significant when incubating the diarrheal-type. The addition of pH indicators, such as methyl red and phenol red, to the medium allowed visual differentiation between diarrheal- and emetic-type B. cereus. A solid agar medium was also developed by optimizing the concentrations of medium components such as monosaccharides, agar, egg yolk enrichment, pH indicators, and antibiotics. This study indicates the possibility of applying selective media for the differentiation of diarrheal- and emetic-type B. cereus.

• 전기의세계
• /
• v.29 no.8
• /
• pp.519-523
• /
• 1980

The traditional mechanical manometer is improved to develop more convenient and precise manometer in continuous measurement of the gas pressure in medium-low vacuum range (1x10$^{-1}$ -10 Torr.) Glass (solid) is used as a detector material of the improved manometer. Using the strain gauge adhered to thin glass board, mechanical strain corresponding to variation of pressure in measurement system is converted into quantity of electricity, and the quantity of electricity is amplified. Experiments have also shown that the improved manometer have more advantages in reproducibility, measured sensibility, and responsible velocity than taditional one.

• Journal of Korean Society for Quality Management
• /
• v.32 no.3
• /
• pp.94-108
• /
• 2004

TQM has been applied to the industry area regardless of the size of company. BNQA model should be identically applied to large companies and small & medium-sized company. However, there are many evidences that TQM model should be modified according to the size of company. In this study, we analyzed the practice of TQM in Korean small & medium-sized Company. Also, we tried to give some suggestions for improving the competitiveness of the small & medium-sized company. The result of this study is that leadership, work system, education and strategy development should be improved in domestic companies, and work system, education and strategy deployment should be improved in foreign companies.

• Journal of Plant Biotechnology
• /
• v.36 no.2
• /
• pp.184-192
• /
• 2009

The influences of pretreatment medium, the addition of fresh medium, and the size of culture plate on the production of embryos were investigated in isolated microspore culture of hot pepper (Capsicum annuum L.). Among the media used for heat shock pretreatment ($32{\pm}1{^{\circ}C}$), high frequency embryo production was obtained when the sucrosestarvation medium A was used. On the other hand, neither 0.37 M mannitol solution nor NLNS medium supplemented with sucrose was not efficient for embryo production. The addition of culture medium to pretreatment media considerably decreased the embryo production even though embryo development proceeded further. The embryo production was not improved by the addition of fresh medium after 2 or 3 weeks from starting culture. Increase in the size of the culture plate from $3.5{\times}1.0$ cm to $6.0{\times}1.5$ cm improved embryo quality. These results will provide valuable information for developing an efficient microspore culture system of hot pepper for high frequency embryo production.

• Journal of Animal Science and Technology
• /
• v.63 no.3
• /
• pp.603-613
• /
• 2021

This research improved the growth potential of Bifidobacterium animalis subsp lactis strain JNU306, a commercial medium that is appropriate for large-scale production, in yeast extract, soy peptone, glucose, L-cysteine, and ferrous sulfate. Response surface methodology (RSM) was used to optimize the components of this medium, using a central composite design and subsequent analyses. A second-order polynomial regression model, which was fitted to the data at first, significantly lacked fitness. Thus, through further analyses, the model with linear and quadratic terms plus two-way, three-way, and four-way interactions was selected as the final model. Through this model, the optimized medium composition was found as 2.8791% yeast extract, 2.8030% peptone soy, 0.6196% glucose, 0.2823% L-cysteine, and 0.0055% ferrous sulfate, w/v. This optimized medium ensured that the maximum biomass was no lower than the biomass from the commonly used blood-liver (BL) medium. The application of RSM improved the biomass production of this strain in a more cost-effective way by creating an optimum medium. This result shows that B. animalis subsp lactis JNU306 may be used as a commercial starter culture in manufacturing probiotics, including dairy products.

• Journal of the Korean Wood Science and Technology
• /
• v.49 no.5
• /
• pp.453-461
• /
• 2021

In wood-based composite panels, low-molar ratio (LMR) urea-formaldehyde (UF) resins usually result in reduced formaldehyde emission (FE) at the expense of poor adhesion. However, the FE and adhesion of medium-density fiberboard (MDF) bonded with LMR UF resins were both improved in this study. The modified LMR UF resins with transition metal ion-modified bentonite (TMI-BNT) nanoclay simultaneously improved the FE and adhesion of MDF panels. The modified LMR UF resins with 5% TMI-BNT resulted in a 37.1% FE reduction and 102.6% increase in the internal bonding (IB) strength of MDF panels. Furthermore, thickness swelling and water absorption also significantly decreased to 13.0% and 24.9%, respectively. These results imply that TMI-BNT modification of LMR UF resins could enhance the formation of a three-dimensional network rather than crystalline domains, resulting in improved cohesion.

• Korean Journal of Food Science and Technology
• /
• v.26 no.5
• /
• pp.526-531
• /
• 1994

In order to develop a new improved selective medium for the Bifidobacterium sp. from the human fecal samples, one hundred eight Bifidobacterium strains were isolated and identified. Sensitivity test for the antibiotics and antimetabolites and test for the specific substrate were performed to obtain basic data for the development of the Bifidobacterium selective medium. TOS(transgalactosylated oligosaccharide) was shown to be preferentially used by Bifidobacterium sp.. Sodium propionate promoted the growth of Bifidobacterium while inhibiting other intestinal bacteria. Upon these results, TP medium was designed and shown to be very effective for the selection of Bifidobacterium and better than Mitsuoka BS medium.

• The Transactions of the Korean Institute of Power Electronics
• /
• v.22 no.4
• /
• pp.285-296
• /
• 2017

This paper proposes an improved modulation scheme for the medium voltage modular multi-level converter (MMC), which operates in the nearest level control and applies in the medium voltage direct current (MVDC) system. In the proposed modulation scheme, the offset (neutral-to-zero output) voltage is adjusted, with the phase voltage magnitude, thereby maintaining a constant value with N+1 level in the controllable modulation index (MI) range. In order to confirm the proposed scheme's validity, computer simulations for the 22.9 kV - 25 MVA MMC were performed with PSCAD/EMTDC, as well as hardware experiments for the 380 V - 10 kVA MMC. The proposed modulation scheme offers to build a constant pole voltage regardless of the MI value, and to build a phase voltage with improved total harmonic distortion (THD).

• Journal of Korea Multimedia Society
• /
• v.7 no.3
• /
• pp.340-348
• /
• 2004

In this paper, we proposed an efficient medium access protocol to share single transmission medium for several stations. There are two well known types of medium access protocols: collision allowance and collision avoidance, and each of them is adopted to their own suitable network with advantage. Our proposed protocol have channel reservation facility based on collision allow medium access protocol, CSMA/CD. In a collision, the stations delay collision with generating pseudo collision signal to reserve channel. Then, the collision terminates and the stations, which reserve channel successfully, participate the next competition with higher medium access probability. We show that the proposed protocol reduced collision times and improved utilization of the channel.