• Clinical and Experimental Pediatrics
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• 제49권10호
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• pp.1061-1066
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• 2006

목 적 : 신생아에서 우유 알레르기가 의심되는 경우 우유 특이 IgE 항체 측정이 우유 알레르기 진단에 유용한지 알아보고자 하였다. 또한 추적관찰이 가능한 경우 우유 특이 IgE 항체 검사결과와 다른 알레르기 질환의 발현과의 연관성도 알아보고자 하였다. 방 법 : 생후 4주이내의 신생아로 우유 특이 IgE 항체 검사양성을 보인 87명을 대상으로 하였다. 대상 환아는 병력 청취와 진찰 소견에서 우유 알레르기가 의심되어 우유 단백 제거 요법을 시행하여 증세의 호전을 보이고 우유 특이 IgE 항체 검사에서 양성을 보인 환아로 하였다. 또한 이들 87명의 환아 중 추적관찰이 가능했던 40명을 대상으로 우유 특이 IgE 항체 검사결과와 알레르기 질환 발현과의 상관관계를 알아보았고 이들 항체와 알레르기 질환의 가족력과의 상관관계도 알아보았다. 결 과 : 대상 환아의 평균 나이는 $17.2{\pm}5.4$일 이었다. 우유 특이 IgE 항체 검사 결과 milk 양성은 87명이었고 casein 양성은 24명, ${\alpha}$-lactoalbuminn 양성은 38명, ${\beta}$-lactoglobulin 양성은 75명이었다. 6개월 이상의 추적관찰이 가능했던 환아는 40명(45.9%)이었고 부모가 알레르기 질환을 가진 경우는 10명(25%), 환아의 추적 관찰 결과 알레르기 질환을 가진 경우가 15명(37.5%)으로 천식 4명, 아토피 피부염 11명이었다. 추적관찰 결과 신생아기에 casein에 양성을 보인 경우 알레르기 질환의 발현과 연관성이 통계적으로 유의하게 높았다. 그러나 우유 특이 IgE 항체 양성과 알레르기 가족력과의 연관성은 통계적 유의성이 없었다. 결 론 : 신생아에서 IgE 매개성 및 혼합성 우유 알레르기가 의심되는 경우는 우유 제거 식이 요법과 우유 특이 IgE 항체 검사를 시행한다면 진단에 도움이 될 수 있다고 생각된다.

• 한국식품영양과학회지
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• 제44권8호
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• pp.1121-1127
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• 2015

2,4-Dinitrochlorobenzene(DNCB) 유도 아토피 피부염 BALB/c 모델에서 외톨개 모자반 에탄올 추출물(MMEE)의 항아토피 효과를 알아보기 위해 육안평가, severity score, 혈청 내 total immunoglobulin E(IgE), interleukin(IL)-4, tumor necrosis $factor-{\alpha}$($TNF-{\alpha}$), IL-10 분비량 및 비장 세포 배양액 내 IL-4, IL-5, IL-13, $interferon-{\gamma}$($IFN-{\gamma}$) 분비량을 측정하였다. DNCB 반복 도포로 아토피 피부염 증상인 건조, 홍반, 짓무름 등의 증상이 두드러지게 나타남을 확인하였고, 이러한 아토피 피부염의 증상은 MMEE의 처리로 인해 증상이 감소되는 것으로 나타났다. Severity score에서도 MMEE의 처리로 인해 DNCB 단독 처리군에 비해 점수가 낮아짐을 확인하였다. 혈청에서 total IgE 및 cytokine의 분비량을 측정한 결과 total IgE, $TNF-{\alpha}$, IL-4의 분비량은 DNCB 단독 처리군에서 증가하였으나 MMEE 처리구에서 normal 군과 유사한 수준으로 감소됨을 확인하였다. 반면 IL-10의 분비량은 DNCB 단독 처리군에서는 normal 군에 비해 감소하였으나 MMEE 처리구에서는 증가하였다. 비장세포 배양액 내에서 cytokine의 분비량을 측정한 결과 IL-4, IL-13 및 IL-5의 분비량은 DNCB 단독 처리군에서는 증가하였으나 MMEE 처리구에서는 normal 군과 유사한 수준으로 감소하였으며, $IFN-{\gamma}$의 분비량은 DNCB 단독 처리군에서는 감소하였으나 MMEE 처리구에서는 증가하여 정상군과 유의적인 차이를 보이지 않았다. 따라서 MMEE는 DNCB 유도 아토피 피부염 동물 모델에서 Th1/Th2 cytokine의 활성 조절 및 total IgE 분비 억제를 통해 아토피 피부염 증상 개선에 효과를 가지는 것으로 사료된다.

• Food Science and Biotechnology
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• 제15권5호
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• pp.694-697
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• 2006

The initiation of immunoglobulin E (IgE)-mediated allergic reactions requires binding of IgE antibody to its high-affinity receptor. Human basophilic KU812F cells express $Fc{\varepsilon}RI$ on the cell surface and act as effector cells in the allergic response. In this study, we investigated the effects of Scutellaria radix extract on the expression of the $Fc{\varepsilon}RI$ in human KU812F cells. Flow cytometric analysis showed that S. radix extract treatment caused a concentration-dependent decrease in $Fc{\varepsilon}RI$ expression on the cell surface. Furthermore, the level of $Fc{\varepsilon}RI$ ${\alpha}$, ${\beta}$, and ${\gamma}$ chain mRNA in KU812F cells was examined by RT-PCR. S. radix extract reduced total cellular $Fc{\varepsilon}RI$ $\alpha$ and ${\gamma}$ chain mRNA expression in a concentration-dependent manner. $Fc{\varepsilon}RI$-mediated histamine release was reduced from $21.75{\pm}1.34\;ng/10^6$ cells in non-treated cells to $16.46{\pm}1.98\;ng/10^6$ cells in S. radix extract treated cells. These results suggested that S. radix extract has the potential to down-regulate of FcRI expression and to inactivate basophils.

• 대한본초학회지
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• 제27권1호
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• pp.11-16
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• 2012

Objectives : In the theory of Korean medicine, Pear has long been considered to protect throat, bronchus and lung. Pear has been believed to remove sputum in Korean people. This study was conducted to investigate the effect of pear ethanol extract (PEE) on asthma induced by ovalbumin in mice. Methods : We investigated the effects of PEE on airway hyperresponsiveness to methacholine, production levels of ovalbumin (OVA) specific total immunoglobulin G (IgG), IgG1, IgG2a and IgE in serum and histopathological changes of lung tissues in asthamtic mice. Results : PEE decreased airway hyperresponsiveness to methacholine significantly compared to non-treated asthmatic mice (P<0.05). Level of OVA specific IgE in serum was lowered by oral administration of PEE effectively (P<0.05). In histopathological observation, administration of PEE reduced infiltration of immune cells into lung tissue. Conclusion : These results suggest that pear has anti-asthmaitc action and related mechanims are involved in anti-inflammatory action such as reducing level of OVA specific IgE and immune cell infiltration.

• 동의생리병리학회지
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• 제18권6호
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• pp.1869-1880
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• 2004

In the oriental medicine, a mixture of herbs has been commonly used as important components to control allergic and inflammatory diseases. In the present study, we prepared a mixture of Dictamni Radicis Cortex(Baiksunpee), Houttuyniae Herba(Uhsungcho), and Aurantii Immaturus Fructus(Jisil) to examine its anti-allergic effects in activated mouse splenic cells and found that Mix-1 is involved in regulating levels of B cell activating factors (CD23 and CD11a), IL-1β, IL-6, IL-10, TNF-α, and 1gE as well as HRF expression. It was observed that Mix-1 did not have cytotoxic effects on mLFC. Mix-1 showed inhibition of CD23 and CD11 alpaha expression in mouse B cells, and also decreased the production of IL-6, TNF-α, and 1gE. Both RT-PCR and ELISA analyses indicated that IL-6 and TNF alpha production were regulated at the gene expression level. In contrast, IL-10 mRNA and protein levels were increased in activated B cells by Mix-1 treatment. We also found that Mix-1 inhibited B cell proliferation and inhibited histamine releasing factor(HRF) expression, suggesting its inhibitory effect on histamine secretion. These data indicated that Mix-1 has an anti-allergic effect in activated macrophages and further suggest the possible application of Mix-1 as a therapeutic agent for the treatment of allergy-related diseases.

• 한국식품과학회지
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• 제43권2호
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• pp.220-223
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• 2011

KU812F 세포는 세포표면에 $Fc{\varepsilon}RI$을 발현하고 있어, IgE를 매개로 한 알러지 반응에 있어 중요한 역할을 담당하고 있는 것으로 알려져 있다. KU812F 세포를 이용하여, $Fc{\varepsilon}RI$ ${\alpha}$ chain 발현에 있어 구절초 에탄올 추출물의 저해효과를 조사하였다. 본 연구 결과, $Fc{\varepsilon}RI$ ${\alpha}$ chain의 세포표면 발현량 및 mRNA수준을 구절초 추출물이 감소시키는 것을 flow cytometry 및 RT-PCR로 각각 확인하였다. 또한, 구절초 추출물은 CRA-1 자극에 의한 히스타민 유리를 농도 의존적으로 억제시켰다. 이러한 결과는 구절초 추출물이 $Fc{\varepsilon}RI$ ${\alpha}$ chain 발현을 감소시킴으로써, 히스타민 유리가 억제되고, 이를 통해 항알러지 활성에 기여하는 것으로 생각된다.

• Clinical and Experimental Pediatrics
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• 제54권10호
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• pp.414-421
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• 2011

Purpose: Recently, an increase in the number of patients sensitized to rice allergen with or without clinical symptoms has been reported. This study was designed to determine the major allergens in rice and their clinical significance. Methods: Twenty-four children (15 boys and 9 girls; mean age, 16.3 months) with allergic disease, who were sensitized to rice antigen (by UniCAP) in the Pediatric Allergy Respiratory Center at Soonchunhyang University Hospital, were enrolled in this study. The allergenicity of various types of rice (raw, cooked, and heat-treated, simulated gastric fluid [SGF], and simulated intestinal fluid [SIF]) was investigated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoglobulin E (IgE) immunoblots. The patients' medical records, including laboratory data and allergy symptoms after ingestion of rice were reviewed. Results: Patients were sensitized to an average of 13.5 food antigens and their mean total IgE was 6,888.7 kU/L. In SDS-PAGE, more than 16 protein bands were observed in the raw rice, whereas only 14-16 kDa and 31-35 kDa protein bands were observed in cooked rice. The common SDS-PAGE protein bands observed in SGF-, SIF-, and heat-treated rice were 9, 14, and 31 kDa. In a heated-rice IgE immunoblot, protein bands of 9, 14, and 31-33 kDa were found in 27.8%, 38.9%, and 38.9% of all sera, respectively, and in 50%, 50%, and 75%, of ser a from the 4 symptomatic patients, respectively. Conclusion: The 9-, 14-, and 31-kDa protein bands appeared to be the major allergens responsible for rice allergy symptoms.

• Journal of Microbiology and Biotechnology
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• 제14권3호
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• pp.490-497
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• 2004

Although the E. coli heat-labile enterotoxin B subunit (LTB) is known to be a potent mucosal adjuvant towards co-administrated unrelated antigens and immunoregulator in T-helper 1-type-mediated autoimmune diseases, a more efficient and useful LTB is still required for prospective vaccine adjuvants. To determine whether a novel chimeric LTB subunit would produce an enhanced mucosal adjuvant activity and immune response, a number of LTB subunits were genetically fused with chimeric proteins using the epitope genes of the envelope glycoprotein E2 (gp51-54) from the classical swine fever virus (CSFV). It was found that the total serum immunoglobulin (Ig) levels of BALB/c mice orally immunized with chimeric proteins containing an N-terminal linked LTB subunit (LE1, LE2, and LE3) were higher than those of mice immunized with LTB, E2 epitope, and chimeric proteins that contained a C-terminal linked LTB subunit. In particular, immunization with LE1 markedly increased both the total serum Ig and fecal IgA level compared to immunization with LTB or the E2 epitope. Accordingly, the current results demonstrated that the LTB subunit in a chimeric protein exhibited a strong mucosal adjuvant effect as a carrier molecule, while the chimeric protein containing the LTB subunit stimulated the mucosal immune system by mediating the induction of antigen-specific serum Ig and mucosal IgA. Consequently, an LE1-mediated mucosal response may contribute to the development of effective antidiarrhea vaccine adjuvants.

• Parasites, Hosts and Diseases
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• 제53권6호
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• pp.675-682
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• 2015

The effects of diclazuril on the bursa of Fabricius (BF) structure and secretory IgA (SIgA) expression in chickens infected with Eimeria tenella were examined. The morphology of the BF was observed by hematoxylin and eosin staining, while ultrastructural changes were monitored by transmission electron microscopy. E. tenella infection caused the BF cell volumes to decrease, irregularly arranged, as well as, enlargement of the intercellular space. Diclazuril treatment alleviated the physical signs of damages associated with E. tenella infection. The SIgA expression in BF was analyzed by immunohistochemistry technique. The SIgA expression increased significantly by 350.4% (P<0.01) after E. tenella infection compared to the normal control group. With the treatment of diclazuril, the SIgA was relatively fewer in the cortex, and the expression level was significantly decreased by 46.7% (P<0.01) compared with the infected and untreated group. In conclusion, E. tenella infection in chickens induced obvious harmful changes in BF morphological structure and stimulated the expression of SIgA in the BF. Diclazuril treatment effectively alleviated the morphological changes. This result demonstrates a method to develop an immunological strategy in coccidiosis control.

• 대한본초학회지
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• 제30권3호
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• pp.1-12
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• 2015

Objectives : In this study, we investigated the effects of Agastachis Herba water (AH-W) extract on compound 48/80-induced mast cell degranulation and histamine release in human mast cells and also anti-asthmatic effect of AH-W extract on ovalbumin (OVA)-induced asthma in mice. Methods : Human mast cells, HMC-1 were treated with AH-W extract in the presence or absence of compound 48/80 (C48/80). Mast cell degranulation was observed by microscope, and the histamine release was measured in culture medium by ELISA. For preparation of asthmatic in vivo model, mice were sensitized (0, 7, and 14 days) with OVA and airway challenged (21, 23, 25, 27, and 29 days). AH-W extract at doses of 100 and 300 mg/kg/body weight was orally administered during OVA challenge once per a day. The levels of immunoglobulin (Ig) E, and Th1/Th2 cytokines, IFN-$\gamma$ and IL-4 were measured in the sera of mice by ELISA. The histopathological change of lung tissues was observed by hematoxylin and eosin (H&E) and Periodic Acid Schiff (PAS) staining. Results : The treatment of AH-W extract significantly decreased the mast cell degranulation and histamine release in C48/80-stimulated HMC-1 cells. In addition, The administration of AH-W extract at does of 100 and 300 mg/kg significantly decreased the serum levels of OVA-specific IgE compared with those of OVA control group. In H&E and PAS staining, AH-W extract inhibited OVA-induced airway inflammation, and inflammatory cells infiltration, and also histopathological damages on lung tissues such as bronchiole epithelial desquamation, goblet cells hyperplasia, and mucin releasing. Conclusions : These results indicate that AH-W extract may improve asthmatic symptoms through mast cell stabilization and inhibiting the lung inflammation in bronchial asthma.