Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

Suppressive Effects of Scutellaria radix Water Extract on $Fc{\varepsilon}RI$ Expression

  • Shim, Sun-Yup (Oriental Medicine Research and Development Institute, Pukyong National University) ;
  • Byun, Dae-Seok (Major of Nutrition, Pukyong National University) ;
  • Kim, Hyeung-Rak (Major of Nutrition, Pukyong National University) ;
  • Lee, Kyung-Dong (Department of Oriental Medicine Materials, Dongshin University) ;
  • Cho, Young-Sook (Department of Food and Nutrition, Sunchon National University)
  • Published : 2006.10.30
Download PDF
⟨ Previous Next ⟩

Abstract

The initiation of immunoglobulin E (IgE)-mediated allergic reactions requires binding of IgE antibody to its high-affinity receptor. Human basophilic KU812F cells express $Fc{\varepsilon}RI$ on the cell surface and act as effector cells in the allergic response. In this study, we investigated the effects of Scutellaria radix extract on the expression of the $Fc{\varepsilon}RI$ in human KU812F cells. Flow cytometric analysis showed that S. radix extract treatment caused a concentration-dependent decrease in $Fc{\varepsilon}RI$ expression on the cell surface. Furthermore, the level of $Fc{\varepsilon}RI$ ${\alpha}$, ${\beta}$, and ${\gamma}$ chain mRNA in KU812F cells was examined by RT-PCR. S. radix extract reduced total cellular $Fc{\varepsilon}RI$ $\alpha$ and ${\gamma}$ chain mRNA expression in a concentration-dependent manner. $Fc{\varepsilon}RI$-mediated histamine release was reduced from $21.75{\pm}1.34\;ng/10^6$ cells in non-treated cells to $16.46{\pm}1.98\;ng/10^6$ cells in S. radix extract treated cells. These results suggested that S. radix extract has the potential to down-regulate of FcRI expression and to inactivate basophils.

Keywords

References

  1. Altman AR, Chiaramonte LT. Public perception of food allergy. J. Allergy Clin. Immun. 4: 95-99 (1996)
  2. Marks DR, Marks LM. Food allergy. Manifestations, evaluation, and management. Postgrad. Med. 93: 191-196 (1993) https://doi.org/10.1080/00325481.1993.11701608
  3. Kinet JP. The high affinity receptor for immunoglobulin E. Curr. Opin. Immunol. 2: 499-505 (1990) https://doi.org/10.1016/0952-7915(90)90002-X
  4. Garman SC, Wurzburg BA, Tarchevskaya SS, Kinet JP, Jardetzky TS. Structure of the Fc fragment of human IgE bound to its high-affinity receptor $Fc{\varepsilon}RI{\alpha}$. Nature 406: 259-266 (2000) https://doi.org/10.1038/35018500
  5. Bae JH. Lee MJ. Lee SM. Antimicrobial effect of Scutellaria baicalensis george extracts on food-borne pathogens. Korean J. Microbiol. Biotechnol. 33: 35-40 (2005)
  6. Kubo M, Kimura Y, Odani T. Studeis on Scutellaria radix Part : The antibacterial substance. Planta Med. 43: 194-201 (1981) https://doi.org/10.1055/s-2007-971499
  7. Moon YH, Lee YH, Min BS, Bae KH. Antibacterial constituents from Scutellaria radix against Streptococcus mutants OMZ176. Korean J. Pharma. 28: 99-103 (1997)
  8. Zhang DY, Wu J, Ye F, Xue L, Jiang S, Yi J, Zhang W, Wei H, Sung M, Wang W, Li X. Inhibition of cancer cell proliferation and prostaglandin $E_{2}$ synthesis by Scutellaria baicalensis. Cancer Res. 63: 4037-4043 (2003)
  9. Chi YS, Lim H, Park HI, Kim HP. Effect of wogonin, a plant flavone from Scutellaria radix, on skin inflammation: in vivo regulation of inflammation-associated gene expression. Biochem. Pharmacol. Bull. 66: 1271-1278 (2003) https://doi.org/10.1016/S0006-2952(03)00463-5
  10. Kubo M, Matsuda H, Tanaka M, Kimura Y, Okuda H, Higashino M, Tani T, Namba K, Arichi S. Studies on Scutellaria radix. Anti-arthritic and anti-inflammatory actions of methanolic extract and flavonoid components from Scutellaria radix. Chem. Pharmacol. Bull. 32: 2724-2729 (1984) https://doi.org/10.1248/cpb.32.2724
  11. Park SN. Antioxidative properties of baicalein, component from Scutellaria baicalensis Georgi and its application to cosmetics (I). J. Korean Ind. Eng. Chem. 14: 657-665 (2003)
  12. Rhee JS, Woo ER, Kim NH, Lee EJ, An DK, Lee JH, Park SK, Park HK. A study on quantative and quantitative analysis of major ingredients in Scutallariae raix. Anal. Sci. Tech. 10: 91-104 (1997)
  13. Lia HB, Jiangb Y, Chena F. Separation methods used for Scutellaria baicalensis active components. J. Chromatogr. 812: 277-290 (2004) https://doi.org/10.1016/S1570-0232(04)00545-8
  14. Li HB, Chen F. Isolation and purification of baicalein, wogonin, and oroxylin A from the medicinal plant Scutellaria baicalensis by high-speed counter-current chromatography. Chromatography 1074: 107-110 (2005) https://doi.org/10.1016/j.chroma.2005.03.088
  15. Koda A. Nishiyori T, Nagai H, Matsuura N, Tsuchiya H. Antiallergic actions of traditional oriental medicine-actions against type I and IV hypersensitivity reactions. Nippon Yakugaku Zasshi 80: 31-41 (1982) https://doi.org/10.1254/fpj.80.31
  16. Shore PA, Burkhalter A, Cohn VH, Victor HC. A method for the fluorometric assay of histamine in tissue. J. Pharmacol. Exp. Ther. 127: 182-186 (1959)
  17. Kishi K. A new leukemia cell line with Philadelphia chromosome characterized as basophil precursors. Leukemia Res. 9: 381-390 (1985) https://doi.org/10.1016/0145-2126(85)90060-8
  18. Chen JH, Ho HO, Yen KY, Yan LL. Hepatoprotection by dangquilong-hwei-wan in male mice. Am. J. Chinese Med. 28: 115-121 (2000) https://doi.org/10.1142/S0192415X00000143
  19. Kimura Y, Kubo M, Kusaka K, Tani T, Higashino M, Arichi S, Okuda H. Studies on Scutellaria radix. V. Effects on ethanolinduced hyperlipemia and lipolysis in isolated fat cells. Chem. Pharmacol. Bull. 30: 219-222 (1982) https://doi.org/10.1248/cpb.30.219
  20. Lin CC, Shieh DE. The anti-inflammatory activity of Scutellaria rivularis extracts and its active components, baicalin, baicalein, and wogonin. Am. J. Chinese Med. 24: 31-36 (1996) https://doi.org/10.1142/S0192415X96000050
  21. Nishiyama C, Yokota T, Okumura K, Ra C. The transcription factors Elf-1 and GATA-1 to cell-specific enhancer elements of human high-affinity IgE receptor ${\alpha}$-chain gene. J. Immunol. 163: 623-630 (1999)
  22. Nishiyama C, Hasegawa M, Nishiyama M, Takahashi K, Akizawa Y, Yokota T, Okumura K, Ogawa H, Ra C. Regulation of human $Fc{\varepsilon}RI$ ${\alpha}$-chain gene expression by multiple transcription factors. J. Immunol. 168: 4546-4552 (2002) https://doi.org/10.4049/jimmunol.168.9.4546
  23. Yanagihara Y, Kajiwara K, Basaki Y, Ikizawa K, Akiyama K, Saito H. Induction of human IgE synthesis in B cells by a basophilic cell line. Clin. Exp. Immunol. 108: 295-301 (1997) https://doi.org/10.1046/j.1365-2249.1997.d01-1001.x
  24. Metzger H. The high affinity receptor for IgE on mast cells. Clin. Exp. Immunol. 4: 419-470 (1991)
  25. Yang SO, Ji GO. Inhibitory effects of Ulmus parvifolia and Liriope platyphylla wang et tang on histamine release from rat peritoneal mast cells. Food Sci. Biotechnol. 15: 363-368 (2006)
  26. Lee JM, Kim NJ, Cho D-H, Chung M-Y, Hwang K-T, Kim H-J, Jun WJ, Park C-S. Ethanol extract of Oenenthe javanica modulates inflammatory response by inhibiting NF-${\kappa}B$ mediated cyclooxygenase-2 expression in RAW 264.7 macrophage. Food Sci. Biotechnol. 15: 303-307 (2006)
  27. Choi S-Y, Hwang J-H, Ko H-C, Park S-Y, Kim G-O, Kim D-H, Chang IS, Kwon HM, Kim S-J. Inhibitory action of Tsunokaori Tangor peel on the lipopolysaccharide-induced inflammatory response in RAW264.7 macrophage cells. Food Sci. Biotechnol. 15: 270-276 (2006)
  28. Shim SY, Ichikawa A, Yamashita M, Katakura Y, Teruya K, Mochizuki Y, Tobinaga E, Shirahata S. Construction of an in vitro allergy reaction evaluation system using human leukemia cell lines. Cytotechnology 40: 75-83 (2002) https://doi.org/10.1023/A:1023918206060