• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.34 no.3
• /
• pp.280-287
• /
• 1989

In order to know growing habit of the maize bearing tillers, IK//IRI/B68 with tillers was grown and compared with Jinjoo Ok which is not tillering, and several parameters were measured for analysis. The results obtained were summarized as follows. 1. The plant height and fresh weight of IK//IRI/B68 were lower than those of Jinjoo Ok until the tasseling stage of IK/ /IRI/B68, while then plant height and fresh weight of IK//IRI/B68 were higher than those of Jinjoo Ok after the tasseling stage of IK//IRI/B68. 2. The average number of tillers per plant of IK//IRI/B68 at maturity was 3.7 and the average tiller height of IK//IRI/B68 was almost the same as the height of the main stem. 3. The dry weight of IK//IRI/B68 at the early growing stage was lower than the check hybrid Jinjoo Ok, while it was higher than Jinjoo Ok at the later growing stage. 4. The LAI of IK//IRI/B68 after full expansion of the leaves was greater than that of Jinjoo Ok The LAIs of IK//IRI/B68 and Jinjoo Ok were 12.6 and 5.8, respectively. 5. The CGR of Jinjoo Ok was greater than that of IK//IRI/B68 until earey July while the CGR of Jinjoo Ok was lower than that of IK//IRI/B68 as the tillers of IK//IRI/B68 were fully developed. 6. The LAR and RGR of IK//IRI/B68 were greater than those of Jinjoo Ok until mid-July. The RGR of IK//IRI/B68 seemed to be affected by both NAR and LAR. The RGR of Jinjoo Ok seemed to be affected by LAR.

• Korean journal of applied entomology
• /
• v.45 no.1 s.142
• /
• pp.15-24
• /
• 2006

Inhibitor kB (IkB)-like gene has been found in the genome of Cotesia plutellae bracovirus (CpBV), which is the obligatory symbiont of an endoparsitoid wasp, C. plutellae. The open reading frame of CpBV-IkB was 417 bp and encoded 138 amino acids. Four ankyrin repeat domains were found in CpBV-IkB, which shared high homology with other known polydnavirus IkBs. Considering a presumptive cellular IkB based on Drosophila Cactus, CpBV-IkB exhibited a truncated structure with deletion of signal-receiving domains, which suggested its irreversible inhibitory role in NFkB signal transduction pathway of the parasitized host in response to the wasp parasitization. CpBV-IkB was expressed only in the parasitized diamondback moth, Plutella flostella. Its expression was estimated by quantitative RT-PCR during parasitization period, showing a constitutive expression pattern from the first day of parasitization. An indirect functional analysis of CpBV-IkB was conducted and suggested a hypothesis of host antivirus inhibition.

• Korean Journal of Veterinary Research
• /
• v.45 no.1
• /
• pp.7-15
• /
• 2005

To elucidate the roles of phospho-IkB expression in the development and progression of EAE, we investigated the expression of phospho-IkB in the central nervous system (CNS) of rats during experimental autoimmune encephalomyelitis (EAE) using immunohistochemistry and Western blot analysis. In Western blot analysis, the increased expression of phospho-IkB went parallel to severity of EAE. The expression of phospho-IkB increased significantly at the peak stage of EAE followed by gradual decrease. Immunohistochemical studies showed that the phospho-IkB immunoreactivity was mainly expressed in inflammatory cells (macrophages, T cells) and glial cells (astrocytes, microglial cells) at the peak stage of EAE and disappeared at the recovery stage. These findings suggest that the phosphorylation of IkB is closely associated with autoimmune inflammation in the CNS and plays an important role in the initiation and progression of EAE.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.34 no.4
• /
• pp.364-369
• /
• 1989

Leaf characteristics of main stem and tiller of IK type maize (IK/ /IRI/B68) were compared with those of Jinjoo Ok hybrid which are not usually tillered. A total of nine leaves from flag leaf to the third or the fourth leaf below ear-bearing node were sampled from each stem or tiller. There was no significant difference in mean leaf length between IK/ /IRI/B68 and Jinjoo Ok. But the mean leaf width of IK/ /IRI/ B68 was about 2 cm narrower than that of Jinjoo Ok. The mean leaf area of the IK/ /IRI/B68 was also smaller than that of Jinjoo Ok due to the narrower leaf width. There were not significant differences in mean leaf characteristics between main stem and tillers of IK/IRI/B68. The longest leaf was the leaf below the ear-bearing node and the widest leaf was the leaf just above the ear-bearing node. Mean length. width and area of leaf on main stem and tillers were similar. Coefficients of variation calculated for individual leaf indicated that the leaves near the ear-bearing node were more uniform than others. The leaf area measured was significantly greater than that estimated by formular, length x width x 0.75. New constant to estimate leaf area of tillering maize was derived as 0.8.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
• /
• v.17 no.1
• /
• pp.163-171
• /
• 2004

The flowers of Lonicera japonica Thunb. (Caprifoliaceae) has been used as anti-inflammatory drug in the folk medicine recipe and been proved its anti-inflammatory effect in the oriental medicine. However, the action mechanism of Lonicera japonica that exhibits anti-inflammatory effects has not been determined. Since nitric oxide (NO) is one of the major inflammatory parameter, we studied the effect of aqueous extracts of Lonicera japonica (AELJ) on NO production in lipopolysaccharide (LPS)-stimulated mouse peritoneal macrophages. NO and inducible NO synthase (iNOS) level were significantly reduced in LPS-stimulated macrophages by AELJ compared to those without Electrophoretic mobility shift assay (EMSA) indicated that AELJ blocked the activation of nuclear factor kappa B (NF-kB), which was considered to be a potential transcription factor for the iNOS expression. AELJ also blocked the phosphorylation and degradation of inhibitor of kappa B-alpha (IkB-${\alpha}$). Furthermore, IkB kinase alpha (IKK${\alpha}$), which is known to phosphorylate serine residues of IkB directly, is inhibited by AELJ in vivo and in vitro. These results suggest that AELJ could exert its anti-inflammatory actions by suppressing the synthesis of NO through inhibition of NF-kB activity.

• Journal of the Korean Applied Science and Technology
• /
• v.12 no.1
• /
• pp.125-131
• /
• 1995

Ultra thin films of Tetra-3-hexadecylsulphamoylcopperphthalocyanine(HDSM-CuPc) were formed on various substrates by Langmuir-Blodgett method, where HDSM-CuPc was synthesized by attaching long-chain alkylamine(hexa-decylamine) to CuPc. The reaction product was identified with FT-IR, UV-visible absorption spectroscopies, elemental analysis and thin layer chromatography. The formation of Ultrathin Langmuir-Blodgett(LB) films of HDSM-CuPc was confirmed by FT-IR and UV-visible spectroscopies. A quartz piezoelectric crystal coated with LB films of HDSM-CuPc was examined as a gas sensor for $N0_2$ gas. HDSM-CuPc LB films were transferred to a quartz crystal microbalance(QCM) in the form of Z-type multilayers. Response characteristics of film-coated QCM to $NO_2$ gas concentrations over a range of $100{\sim}600ppm$ have been tested with a thickness of $5{\sim}20$ layers of HDSM-CuPc. Changes in frequency by adsorption of $NO_2$ were increased With the number of LB layers and $NO_2$ concentration, but the response time was slow.

• Journal of Acupuncture Research
• /
• v.22 no.2
• /
• pp.123-132
• /
• 2005

Melittin,cationic 26-amino acid, is the principal component of the bee venom (BV) which has been used for treatment of inflammatory disease such as arthritis rheumatism NF-kB is activated by subsequent release of inhibitory IkB via activation of a multisubunit IkB kinase (IKK). We previously found that melittin bind to the sulfhydryl group of p50, a subunit of NF-kB. Since sulfhydryl group is present in kinase domain of IKKa and IKKb, melittin could modify IKK activity by protein-protein interaction. We therefore examined effect of melittin on IKK activities in sodium nitroprusside (SNP)-stimulated synoviocyte obtained from RA patients. Melittin suppressed the SNP-induced release of IkB resulted in inhibition of DNA binding activity of NF-kB and NF-kB-dependent luciferase activity. Consistent with the inhibitory effect on NF-kB activation, IKKa and IKKb activities were also suppressed by melittin. Surface plasmon resonance analysis realized that melitin binds to IKKa $(Kd\;=\;1.34{\times}10-9M)$ and IKKb$(Kd\;=\;1.0{\times}10-9M)$. Inhibition of IKKa and IKKb resulted in reduction of the SNP-induced production of inflammatory mediators NO and PGE2 generation. The inhibitory effect of melittin on the IKKs activities, binding affinity of melittin to IKKs, and NO and PGE2 generation were blocked by addition of reducing agents dithiothreitol and glutathione. In addition, melittin did not show inhibitory effect in the transfected Synoviocytes with plasmid carrying dominant negative mutant IKKa (C178A) and IKKb (C179A). These results demonstrate that melittin directly binds to sulfhydryl group of IKKs resulting in IkBrelease, thereby inhibits activation of NF-kB and expression of genes involving in the inflammatory responses.

• Proceedings of the KIEE Conference
• /
• 2000.11a
• /
• pp.46-48
• /
• 2000

This paper presents an approach to B/C analysis amenable to evaluate the impact of DSM programs especially on the strategic conservation programs and the load management programs. The proposed approach embedding the existing B/C analyses is applicable to the new electricity market Case studies show the B/C ratio and the avoided cost due to the impact of DSM programs.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.16 no.6
• /
• pp.1284-1290
• /
• 2002

Scrophularia buergeriana Miquel (Scrophulariaceae) has been used as an anti-inflammatory drug in the folk medicine recipe and been proved its anti-inflammatory effect in the oriental medicine. Since nitric oxide (NO) and superoxide anion (O/sub 2//sup -/) are ones of the major inflammatory parameters, we studied the effect of aqueous extracts of Scrophularia buergeriana (SB) on NO and O/sub 2//sup -/ production in lipopolysaccharide (LPS)-stimulated mouse peritoneal macrophages. NO, O/sub 2//sup -/production and inducible NO synthase (iNOS) level were significantly reduced in LPS-activated macrophages by SB compared to those without. Electrophoretic mobility shift assay (EMSA) indicated that SB blocked the activation of NF-kB, which was considered to be a potential transcription factor for the iNOS expression. SB also blocked degradation of IkBα. Furthermore, IkB kinase alpha (IKKα), which phosphorylates serine residues of IkB directly, is inhibited by SB. These results suggest that SB could exert its anti-inflammatory actions by suppressing the activation of NF-kB through inhibition of IKK activity.

• Journal of the Society of Cosmetic Scientists of Korea
• /
• v.32 no.4 s.59
• /
• pp.241-247
• /
• 2006

This work was carried out to investigate the anti-inflammatory effects of Paulownia coreana Uyeki on abirritant, atopy and acne skin. Paulownia coreana Uyeki has been used as a traditional medicine having anti-febrile, anti-inflammation effect in Korea, Paulownia coreana Uyeki loaves were extracted with 70% EtOH. Its superoxide anion radical scavenging activity and inhibitory effect on LPS-induced NO production were examined. The extract inhibitied the generation of NO and $PGE_2$ induced by LPS in the macrophage cell line (Raw 264.7). Consistent with the inhibitory effects on No and $PGE_2$ generation, the extract inhibited expression of iNOS and COX-2. In further study, it was found that the extract prevented $IkB-{\alpha}$ degradation, as demonstrated by western blot analysis of $IkB-{\alpha}$ protein level. However, the extract treatment did not affect cell viability at $100{\mu}g/mL$ concentration in both human skin fibroblast and Raw 264.7 cells in vitro. Thus, the present study suggests that Paulownia coreana Uyeki leaves extract have significant anti-inflammatory activity and potential as an anti-irritation material.