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Gene Structure of Cotesia plutellae Bracovirus (CpBV)-$I_{k}B$ and Its Expression Pattern in the Parasitized Diamondback Moth, Plutella xylostella  

Kim Yong-Gyun (School of Bioresource Sciences, Andong National University)
Basio Neil A. (Bulacan Agricultural State College, Pinaod, San Ildefonso)
Ibrahim Ahmed M.A. (School of Bioresource Sciences, Andong National University)
Bae Sung-Woo (School of Bioresource Sciences, Andong National University)
Publication Information
Korean journal of applied entomology / v.45, no.1, 2006 , pp. 15-24 More about this Journal
Abstract
Inhibitor kB (IkB)-like gene has been found in the genome of Cotesia plutellae bracovirus (CpBV), which is the obligatory symbiont of an endoparsitoid wasp, C. plutellae. The open reading frame of CpBV-IkB was 417 bp and encoded 138 amino acids. Four ankyrin repeat domains were found in CpBV-IkB, which shared high homology with other known polydnavirus IkBs. Considering a presumptive cellular IkB based on Drosophila Cactus, CpBV-IkB exhibited a truncated structure with deletion of signal-receiving domains, which suggested its irreversible inhibitory role in NFkB signal transduction pathway of the parasitized host in response to the wasp parasitization. CpBV-IkB was expressed only in the parasitized diamondback moth, Plutella flostella. Its expression was estimated by quantitative RT-PCR during parasitization period, showing a constitutive expression pattern from the first day of parasitization. An indirect functional analysis of CpBV-IkB was conducted and suggested a hypothesis of host antivirus inhibition.
Keywords
Antivirus; Cotesia plutellae; CpBV; IkB; NFkB; Plutella xylostelia; Polydnavirus;
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1 Andrade, M.A., C. Perez-Iratxeta and C.P. Pomting. 2001. Protein repeats: structure, functions, and evolution. J. Struct. Biol. 134: 117-131   DOI   ScienceOn
2 Beckage, N.E., F.F. Tan, K.W. Schleifer, R.D. Lane and L.L. Cherubin. 1994. Characterization and biological effects of Cotesia congregata polydnavirus on host larvae of the tobacco homworm, Manduca sexta. Arch. Insect Biochem. Physiol. 26: 165 - 195   DOI   ScienceOn
3 Han, Z.S. and T. Ip. 1999. Interaction and specificity of Rel-related proteins in regulating Drosophila immunity gene expression. J. Biol. Chem. 274: 21355-21361   DOI
4 Hoffmann, J.A. 2003. The immune response of Drosophila. Nature 426: 33 -38   DOI   ScienceOn
5 Imler, J.L. and P. Bulet. 2005. Antimicrobial peptide in Drosophila: structures, activities, and gene regulation. Chem Immunol. Allergy 86: 1 - 21
6 Kim, Y. and J. Kim. 2004. Inhibitory effect of Cotesia plutellae bracovirus (CpBV) on development of a non-natural host, Spodoptera exigua. Korean J. Appl. Entomol. 43: 217 - 223
7 Krell, P.J., M.D. Summers and S.B. Vinson. 1982. Virus with a multipartite superhelical DNA genome from the ichneumonid parasitoid Campoletis sonorensis. J. Virol. 43: 859 - 870
8 Lee, S. and Y. Kim. 2004. Juvenile hormone esterase of diamondback moth, Plutella xylostella, and parasitism of Cotesia plutellae. J. Asia-Pacific Entomol. 7: 283-287   DOI
9 Sedgwick, S.G. and S.J. Smerdon. 1999. The ankyrimepeat: a diversity of interactions on a common structural framework. Trends Biochem. Sci. 24; 311 - 316   DOI   ScienceOn
10 Simeonidis, S., S. Liang, G. Chen and D. Thanos. 1997. Cloning and functional characterization of mouse I${\kappa}B{\varepsilon}$. Proc. Natl. Acad. Sci. USA 94: 14372-14377
11 Liu, Z.-P., R.L. Galindo and S.A. Wasserman. 1997. A role for CKII phosphorylation of the cactus PEST domain in dorsoventral patterning of the Drosophila embryo. Genes Dev. 11: 3413-3422   DOI
12 Bae, S. and Y. Kim. 2004. Host physiological changes due to parasitism of a braconid wasp, Cotesia plutellae, on diamondback moth, Plutella xylostella. Comp. Biochem. Physiol. 138A: 39-44
13 Kroemer, J.A. and B.A. Webb. 2004. Polydnavirus and genome: emerging gene families and new insights into polydnavirus replication. Annu. Rev. Entomol. 49: 431-456   DOI   ScienceOn
14 De Gregorio, E., P.T. Spellman, G.M. Rubin and B. Lemaitre. 2001. Genome-wide analysis of the Drosophila immune response by using oligonucleotide arrays. Proc. Natl. Acad. Sci. USA 98: 12590- 12595
15 Senftleben, U., Y. Cao, G. Xiao, F.R. Greten, G. Krahn, G. Bonizzi, Y. Chen, Y. Hu, A. Fong, S.-C. Sun and M. Karin. 2001. Activation by IKKa of a second evolutionarily conserved, NF-$\kappa$B signaling pathway. Science 293: 1495-1499   DOI   ScienceOn
16 Arenzana-Seisdedos, F., P. Turpin, M. Rodriguez, D. Thomas, R.T. Hay, J.L. Virelizier and C. Dargemont. 1997. Nuclear location of I$\kappa$B$\alpha$ promotes active transport of NF$\kappa$B from the nucleus to the cytoplasm. J. Cell Sci. 110: 369-378
17 Choi, J.Y., J.Y. Rho, J.N. Kang, H.J. Shim, S.D. Woo, B.R. Jin, M.S. Li and Y.H. Je. 2005. Genomic segments cloning and analysis of Cotesia plutellae polydnavirus using plasmid capture system. Biochem. Biophys. Res. Commun. 332: 487 - 493   DOI   ScienceOn
18 Sambrook, J., E.F. Fritsh and T. Maniatis. 1989. Molecular cloning. A laboratory manual. 2nd Ed. Cold Spring Harbor Laboratory Press, NY
19 Thoetkiattikul, H., M.H. Beck and M.R. Strand. 2005. Inhibitor $\kappa$B-like proteins from a polydnavirus inhibit NF-$\kappa$B activation and suppress the insect immune response. Proc. Natl. Acad. Sci. USA 102: 11426-11431
20 Uvell, H. and Y. Engstrom. 2003. Functional characterization of a novel promoter element required for an innate immune response in Drosophila. Mol. Cell. Biol. 23: 8272-8281   DOI
21 Sun, S.C. and I. Faye. 1992. Cecropia immunoresponsive factor, an insect immunoresponsive factor with DNA-binding properties similar to nuclear-factor, k${\beta}$. Eur. J. Biochem. 204: 885-892   DOI   ScienceOn
22 Beg, A.A. and A.S. Baldwin, Jr. 1993. The I$\kappa$B proteins: multifunctional regulators of ReI/NF-$\kappa$B transcription factors. Genes Dev. 7: 2064-2070   DOI   ScienceOn
23 Belle, E., N.E. Beckage, J. Rousselet, M. Poirie, F. Lemeunier and J.-M. Drezen. 2002. Visualization of polydnavirus sequences in a parasitoid wasp chromosome. 76: 5793 - 5796   DOI
24 Shirane, M., S. Hatakeyama, K. Hattori, K. Nakayama and K-I. Nakayama. 1999. Common pathway for the ubiquitination of I${\kappa}B{\alpha}$, I${\kappa}B{\beta}$, and I${\kappa}B{\varepsilon}$ mediated by the F-box protein FWD1. J. Biol. Chem. 274: 28169-28174   DOI
25 Irving, P., J.-M. Ubeda, D. Doucet, L. Troxler, M. Lagueux, D. Zachary, J.A. Hoffmann, C. Hetru and M. Meister. 2005. New insights into Drosophila larval haemocyte functions through genome-wide analysis. Cell. Microbiol. 7: 335 - 350
26 Kappler, C., M. Meister, M. Lagueux, E. Gateff, J.A. Hoffmann and J.M. Reichhart. 1993. Insect immunity. Two 17 bp repeats nesting a k$\beta$-related sequence confer inducibility to the diptericin gene and bind a polypeptide in bacteria-challenged Drosophila. EMBO J. 12: 1561-1568
27 Talekar, N.S. and J.C. Yang. 1991. Characteristics of parasitism of diamondback moth by two laval parasites. Entomophaga 36: 95-104   DOI
28 Dushay, M.S., B. Asling and D. Hultmark. 1996. Origins of immunity: Relish, a compound Rei-like genes in the antibacterial defense of Drosophila. Proc Natl. Acad. Sci. USA 93: 10343 -10347
29 Livak, K.J. and T.D. Schmittgen. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delata C(T)) method. Methods 25: 402-408   DOI   ScienceOn
30 Hatada, E.N., A. Nieters, F.G. Wulczyn, M. Naumann, R. Meyer, G. Nucifora, T.W. McKeithan and C. Scheidereit. 1992. The ankyrin repeat domains of the NF-$\kappa$B precursor p105 and the protooncogene bcl-3 act as specific inhibitors of NF-$\kappa$B DNA binding. Proc Natl. Acad. Sci. USA 89: 2489-2493
31 Webb, B.A. 1998. Polydnavirus biology, genome structure, and evolution. pp. 105-139. In The insect viruses, eds. by L.K. Miller and L.A. Ball. Plenum Publishing Corporation, New York
32 Bennett, V. 1992. Ankyrins. J. Biol. Chem. 267: 8703 -8706
33 Zambon, R.A., M. Nandakumar, V.N. Vakharia and L.P. Wu. 2005. The Toll pathway is important for an antiviral response in Drosophila. Proc. Natl. Acad. Sci. USA 102: 7257-7262
34 Basio, N.A. and Y. Kim. 2005. A short review of teratocytes and their characters in Cotesia plutellae (Braconidae: Hymonoptera). J. Asia-Pacific Entomol. 8: 211-217   과학기술학회마을   DOI
35 O'Reilly D.R., L.K. Miller and V.A. Luckow. 1992. Baculovirus expression vectors. A laboratory manual. 347 pp. W.H. Freeman and Company, NY
36 Huxford, T., D.-B. Huang, S. Malek and G. Ghosh. 1998. The crystal structure of the I$\kappa$B$\alpha$/NF-$\kappa$B complex reveals mechanisms of NF-$\kappa$B inactivation. Cell 95: 759-770   DOI   ScienceOn
37 Lin, P.-H., L.H. Huange and R. Steward. 2000. Cactin, a conserved protein that interacts with the Drosophila I$\kappa$B protein cactus and modulates its function. Mech. Dev. 94: 57-65   DOI   ScienceOn
38 Revilla, Y., M. Callejo, J.M. Rodriguez, E. Culebras, M.L. Nogal, M.L. Salas, E. Vinuela and M. Fresno. 1998. Inhibition of nuclear factor KB activation by a virus-encoded I$\kappa$B-like protein. J. Biol. Chem. 273: 5405 - 5411   DOI   ScienceOn
39 Jacobs, M.D. and S.C. Harrison. 1998. Structure of an I$\kappa$B$\alpha$/NF-$\kappa$B complex. Cell 95: 749-758   DOI   ScienceOn
40 Kroemer, J.A. and B.A. Webb. 2005. I$\kappa$B-related vankyrin genes in the Campoletis sonorensis ichnovirus: temporal and tissuespecific patterns of expression in parasitized Heliothis virescens lepidopteran hosts. J. Virol. 79: 7617-7628   DOI   ScienceOn
41 Oh, M.R., S.S. Kim, J.D. Park, J.C. Paik and D.I. Kim. 1997. Biological characteristics of Cotesia plutellae (Hymenoptera: Braconidae), a larval parasitoid of Plutella xylostella (Lepidoptera: Yponomeutidae). Korean J. Entomol. 27: 79-84
42 Baeuerle, P.A. and D. Baltimore. 1988. I$\kappa$B: a specific inhibitor of the NF$\kappa$B transcripttion factor. Science 242: 540-546   DOI
43 Clem, R.J. 2005. The role of apoptosis in defense against baculovirus infection in insects. Curr. Top. Microbiol. Immunol. 289: 113-130   DOI
44 Engstrom, Y., L. Kadalayil, S.C. Sun, C. Samakovlis, D. Hultmark and I. Faye. 1993. k$\beta$-Iike motifs regulate the induction of immune genes in Drosophila. J. Mol. Biol. 232: 327 -333   DOI   ScienceOn
45 Burke, J.R., M.K. Wood, R.-P. Ryseck, S. Walther and C.A. Meyers. 1999. Peptides corresponding to the Nand C termini of I$\kappa$B-${\alpha},\;-{\beta}$, and -$\epsilon$ as probes of the two catalytic subunits of I$\kappa$B kinase, IKK-1 and IKK-2. J. Biol. Chem. 274: 36146-36152   DOI   ScienceOn
46 Espagne, E., C. Dupuy, E. Huguet, L. Cattolico, B. Provost, N. Martins, M. Poirie, G. Periquet and J.M. Drezen. 2004. Genome sequence of a polydnavirus: insights into symbiotic virus evolution. Science 286-289
47 Karin, M. 1999. The beginning of the end: I$\kappa$B kinase (IKK) and NF-$\kappa$B activation. J. Biol. Chem. 274: 27339-27342   DOI
48 Zabel, U. and P.A. Baeuerle. 1990. Purified human I$\kappa$B can rapidly dissociate the complex of the NF$\kappa$B transcription factor with its cognate DNA. Cell 61: 255-265   DOI   ScienceOn
49 Ray, P., D.-E. Zhang, J.A. Elias and A. Ray. 1995. Cloning of a differentially expressed I$\kappa$B-related protein. J. Biol. Chem. 270: 10680-10685   DOI   ScienceOn
50 Latimer, M., M.K. Ernst, L.L. Dunn, M. Drutskaya and N.R. Rice. 1998. The N-terminal domain of I$\kappa$Ba masks the nuclear localization signal(s) of p50 and c-Rel homodimers. Mol. Cell. Biol. 18: 2640-2649   DOI