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Expression and localization of phospho-IkB in the central nervous system during experimental autoimmune encephalomyelitis in Lewis rats  

Hwang, In Sun (Department of Veterinary Medicine, College of Agriculture and Life Science, Cheju National University)
Jee, Young Heun (Department of Veterinary Medicine, College of Agriculture and Life Science, Cheju National University)
Publication Information
Korean Journal of Veterinary Research / v.45, no.1, 2005 , pp. 7-15 More about this Journal
Abstract
To elucidate the roles of phospho-IkB expression in the development and progression of EAE, we investigated the expression of phospho-IkB in the central nervous system (CNS) of rats during experimental autoimmune encephalomyelitis (EAE) using immunohistochemistry and Western blot analysis. In Western blot analysis, the increased expression of phospho-IkB went parallel to severity of EAE. The expression of phospho-IkB increased significantly at the peak stage of EAE followed by gradual decrease. Immunohistochemical studies showed that the phospho-IkB immunoreactivity was mainly expressed in inflammatory cells (macrophages, T cells) and glial cells (astrocytes, microglial cells) at the peak stage of EAE and disappeared at the recovery stage. These findings suggest that the phosphorylation of IkB is closely associated with autoimmune inflammation in the CNS and plays an important role in the initiation and progression of EAE.
Keywords
experimental autoimmune encephalomyelitis (EAE); phospho-IkB; inflammatory cells; glial cells;
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