• Title/Summary/Keyword: ITS 변이

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Leaf variants of Pinus and their ITS DNA sequences (소나무속 잎 변이와 그의 ITS DNA 염기서열)

  • Koo, JaChoon;Whang, Sung Soo
    • Korean Journal of Plant Taxonomy
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    • v.43 no.1
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    • pp.63-68
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    • 2013
  • ITS DNA sequences of two variants of Pinus spp. having single fasciculate leaf or two to three fasciculate leaves within an individual were analysed in order to determine their origin. Also, the same DNA locus of P. densiflora, P. rigida and P. koraiensis, distributed at the same region together with the OTUs having leaf variations, were analysed to compare with each other. Aligned sequences including ITS1, 5.8S and ITS2 were 580~584 bp in length. The 5.8S region was well conserved among all the OTUs we tested except for P. koraiensis, which has two nucleotide substitutions. The partial ITS1 region upstream of the 5.8S region showed relatively high sequence diversity compare to the ITS2 and has 181~185 bp in length. In this region, the sequences of the two variants were identical to that of P. densiflora. The ITS2 has identical for all OTUs in length and the two variants also have same sequences compare to that of P. densiflora. These two variants and samples of P. densiflora were grouped together in the UPGMA tree with 100% similarity level. The result strongly suggest that these two variants were originated from P. densiflora.

Genetic Variation Based on Random Amplified Polymorphic DNA (RAPD) and Internal Transcribed Spacer (ITS) Region Sequences in Lepista nuda (RAPD와 ITS 영역에 의한 민자주방망이 버섯의 유전적 변이)

  • Lee, Yang Suk;Kim, Nam Woo;Kim, Jong Bong
    • Journal of Life Science
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    • v.22 no.11
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    • pp.1470-1476
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    • 2012
  • A genetic variation of Lepista nuda and two genus Lepista species (L. irina and L. sordida) were analyzed by random amplified polymorphic DNA (RAPD) and internal transcribed spacer (ITS) sequence analysis. In the resulting RAPD analysis, 22 out of 40 random primers amplified polymorphic RAPD fragment patterns, the amplified bands were 355, and DNA fragment sizes were 200-400bp. Intraspecific genetic dissimilarity of the 10 L. nuda strains were calculated to range from 0% to 21.60%, L. sordida from 16.93% to 24.82%, L. irina were 20.62% to 25.54%, and intraspecific genetic dissimilarity of L. sordida and L. irina was 23.49%. The 673 base pairs were sequenced during the analysis of the ITS I and II region; six L. nuda strains intraspecific genetic dissimilarities ranged from 1.58% to 11.47%, L. nuda and L. sordida from 3.83% to 12.88%, L. nuda and L. irina from 7.11% to 15.61%, and intra-specific genetic variation between L. sordida and L. irina was 4.79%. The findings showed that RAPD and ITS sequencing could be used for developing molecular genetic markers and screening of unidentified genus Lepista species.

Genetic relationship of Aloe vera 'Saengjang', a new forma, based on cpDNA and ITS sequence variation (cpDNA와 ITS 염기변이에 근거한 신품종 생장알로에 유전적 상관관계)

  • Srikanth, Krishnamoorthy;Jang, Seon Il;Whang, Sung Soo
    • Korean Journal of Plant Taxonomy
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    • v.44 no.4
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    • pp.250-256
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    • 2014
  • This study was carried out to understand the genetic relationship of three Aloe spp. cultivated in Korea, A. saponaria, A. vera and A. arborescens and a new variant in Korea based on three plastid (matK, trnL-F, rbcL) and one nuclear (ITS regions) DNA barcode markers. A total of 2,420 bp sequence was amplified. Two indels were detected in the trnL region, and also several species specific nucleotide loci were detected in all 29 parsimonious informative sites, and 148 variable sites were detected among four taxa studied while 170 variable and 75 parsimonious sites were detected when other Aloe spp. in worldwide were used. An UPGMA phenogram with 10,000 bootstrap replication showed that the new variant was closest to A. vera. The variant was not morphologically and genetically concurrent with any reported species so far. The clustering of Aloe species were broadly in agreement with previously reported results.

Study on the Meaning of 'Side' in Geometry and Methods of its Introduction in Mathematics Textbook (도형영역에서의 '변(邊)'의 의미 및 도입방법에 대한 고찰)

  • Kim, Soomi
    • Journal of Elementary Mathematics Education in Korea
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    • v.18 no.3
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    • pp.441-457
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    • 2014
  • This study starts with the idea that Korean mathematical word 'byon' which means 'side' of polygons or angles is very ambiguous. The purpose of this study is to make the concept and range of 'byon' clear and to suggest the ideas which can help children understand the concept of 'byon'. For this, various dictionary and the past Korean mathematics curriculums are reviewed. As a result, two attributes 'byon' has are identified and some reasons which block children from understanding 'byon' are detected in its introduction method of mathematics text books and inkhorn of the mathematical term. Finally, two different ideas for helping children understand the concept 'byon' are suggested based on the conclusion of this research.

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Variation of the Axillary Arch in Korean Cadaver (한국인 시신에서 랑거겨드랑활의 변이)

  • Jeong, Hyun-Seok;Jo, Seong-Woo;Lee, Jae-Ho
    • Anatomy & Biological Anthropology
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    • v.31 no.4
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    • pp.167-170
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    • 2018
  • Axillary arch is relatively common variation of muscle in the axilla. There were several attentions on axillary arch due to its anatomical and surgical importance. During educational dissection, a variant muscle was found in right arm of 68-year-old female cadaver. The variation of muscle originated from the lateral edge of the latissimus dorsi muscle as muscular form. And then, it crossed the axillary artery and median nerve as tendinous form. Finally it became wide as muscular form and inserted into the pectoralis major. We reported this variant muscle and discussed its clinical significances.

Bidirectional Dynamic Programming Based Disparity Estimation and its application to the Intermediate View Reconstruction of 3D Images (양방향 다이나믹 프로그래밍 기반 변이 추정을 이용한 3차원 영상의 중간 시점 영상 합성)

  • 배진환;김성식;손정영;손광훈
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.26 no.7B
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    • pp.975-982
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    • 2001
  • 본 논문에서는 3차원 영상 시스템에서 기준이 되는 좌우 영상이 있을 때 임의의 중간 시점 영상 합성 기법을 제안하였다. 제안한 기법은 양방향 다이나믹 프로그래밍 기반 변이 추정 과정과, 이를 기반으로 양방향 변이 정합과정에 의해 가려진 영역을 추출하는 과정, 그리고 완성된 변이 맵을 바탕으로 중간 시점 영상을 생성하는 과정으로 구성된다. 이중 변이 추정 과정에 제안된 양방향 다이나믹 프로그래밍 기법은, 일반적인 다이나믹 프로그래밍이 가진 장점을 살리면서, 영상 특성을 고려한 코스트 값의 사용 및 양방향 코스트 행렬 생성 방법으로 발생될 수 있는 오류를 최소화함으로써 가려진 영역을 보다 정확하게 구분해 낼 수 있었다. 이후 이러한 과정에서 생성된 변이 맵에 대해 적절한 후처리 과정으로 가려진 영역을 판별하고, 이를 기반으로 한 중간 영상 생성에서 모호한 영역을 고려하여 보다 정확하고 안정적인 결과를 얻을 수 있었다.

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Disparity Estimation using a Region-Dividing Technique and Edge-preserving Regularization (영역 분할 기법과 경계 보존 변이 평활화를 이용한 스테레오 영상의 변이 추정)

  • 김한성;손광훈
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.41 no.6
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    • pp.25-32
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    • 2004
  • We propose a hierarchical disparity estimation algorithm with edge-preserving energy-based regularization. Initial disparity vectors are obtained from downsampled stereo images using a feature-based region-dividing disparity estimation technique. Dense disparities are estimated from these initial vectors with shape-adaptive windows in full resolution images. Finally, the vector fields are regularized with the minimization of the energy functional which considers both fidelity and smoothness of the fields. The first two steps provide highly reliable disparity vectors, so that local minimum problem can be avoided in regularization step. The proposed algorithm generates accurate disparity map which is smooth inside objects while preserving its discontinuities in boundaries. Experimental results are presented to illustrate the capabilities of the proposed disparity estimation technique.

The Identity of the Variation Population of Polygonatum cryptanthum H. Lév. & Vaniot (목포용둥굴레 변이 집단의 실체)

  • Se Ryeong Lee;Chang Gee Jang
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2022.09a
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    • pp.50-50
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    • 2022
  • 비짜루과 둥굴레속(Asparagaceae: Polygonatum)은 전 세계적으로 약 90여 종이 알려져 있으며, 유럽, 북아메리카, 아시아 등 북반구 온대 지역에 집중적으로 분포한다. 국내 둥굴레속 분류군은 총 16분류군이며, 이중 잎이 호생하고, 난형에서 타원형 모양의 엽질성 포를 가지며, 화피통 내부에 털이 없고, 수술대 표면에 돌기가 나있는 분류군들은 용둥굴레열(series. Bracteata)에 속한다. 그러나 이들은 종간 교잡 또는 주요 기관의 형질 변이가 다양하여 중간형질을 보이는 개체군들에 대한 종 식별에 많은 어려움이 있었다. 경남 창원시에서 채집된 목포용둥굴레(P. cryptanthum) 변이 개체집단는 기존의 목포용둥굴레와 달리 식물체 높이와 화경·소화경이 길며, 포 부착위치의 변이 폭이 넓으며, 포가 타원형이고 밖으로 말리는 습성으로 형태적 차이가 나타났다. 따라서 본 연구에서는 명확한 분류학적 실체를 구명하고자 분자생물학적 계통분석(nrDNA ITS + cpDNA matK, trnK-rps16, rps16, rbcL) 연구를 진행중에 있다.

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Phylogenic Study of Genus Phyllostachys (Phyllostachys) in Korea by Internal Transcribed Spacer Sequence (ITS) (ITS 서열에 의한 한국 왕대속 식물종의 계통분류학적 연구)

  • Lee, Song-Jin;Huh, Man-Kyu;Huh, Hong-Wook;Lee, Byeong-Ryong
    • Journal of Life Science
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    • v.21 no.9
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    • pp.1281-1287
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    • 2011
  • Phyllostachys consists of high and fast growing trees and is a genus in the family Gramineae. The genus has many species in Asia, with main distribution being in India and China. One of the most popular sequences for phylogenetic inference at the generic and infrageneric levels in plants is the internal transcribed spacer (ITS) region of the 18S-5.8S-26S nuclear ribosomal cistron. We evaluated four taxa with the ITS region to estimate phenotypic relationships within the genus Phyllostachys in Korea. Alignment of the DNA sequences required the addition of numerous gaps. Sequence variation within the Phyllostachys was mostly due to natural selection, although several indels and inserts were found. Within the genus Phyllostachys, P. nigra and P. nigra var. henonis were the relatives in the three phylogenetic analyses (MP, ML, and NJ). However, some external nodes were poorly supported. Morphological traits and simple repeats (ISSR) represented the result of a relationship similar to the that of ITS sequences in the genus Phyllostachys. This suggests that ITS sequences are very informative for identification of these taxa.