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http://dx.doi.org/10.5352/JLS.2011.21.9.1281

Phylogenic Study of Genus Phyllostachys (Phyllostachys) in Korea by Internal Transcribed Spacer Sequence (ITS)  

Lee, Song-Jin (Department of Science Education, Busan National University)
Huh, Man-Kyu (Department of Molecular Biology, Dongeui University)
Huh, Hong-Wook (Department of Science Education, Busan National University)
Lee, Byeong-Ryong (Department of Science Education (Biology), Seowon University)
Publication Information
Journal of Life Science / v.21, no.9, 2011 , pp. 1281-1287 More about this Journal
Abstract
Phyllostachys consists of high and fast growing trees and is a genus in the family Gramineae. The genus has many species in Asia, with main distribution being in India and China. One of the most popular sequences for phylogenetic inference at the generic and infrageneric levels in plants is the internal transcribed spacer (ITS) region of the 18S-5.8S-26S nuclear ribosomal cistron. We evaluated four taxa with the ITS region to estimate phenotypic relationships within the genus Phyllostachys in Korea. Alignment of the DNA sequences required the addition of numerous gaps. Sequence variation within the Phyllostachys was mostly due to natural selection, although several indels and inserts were found. Within the genus Phyllostachys, P. nigra and P. nigra var. henonis were the relatives in the three phylogenetic analyses (MP, ML, and NJ). However, some external nodes were poorly supported. Morphological traits and simple repeats (ISSR) represented the result of a relationship similar to the that of ITS sequences in the genus Phyllostachys. This suggests that ITS sequences are very informative for identification of these taxa.
Keywords
Genus Phyllostachys; internal transcribed spacer (ITS); phylogenetic analyses;
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