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Comparison of Germination Characteristics and Daily Seed Germinating Pattern in Fine-textured Fescues (세엽형 훼스큐속 잔디의 발아특성 및 일일 발아패턴 비교)

  • Kim, Kyoung-Nam;Park, So-Hyang
    • Horticultural Science & Technology
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    • v.28 no.4
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    • pp.567-573
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    • 2010
  • Research was initiated to investigate early establishment characteristics and germination pattern of fine-textured fescues (FF). Six varieties from Chewings fescue ($Festuca$ $rubra$ L. ssp. $commutata$ Gaud., CF), creeping red fescue ($F.$ $rubra$ L. ssp. $rubra$ Gaud., CRF), hard fescue ($F.$ $ovina$ ssp. $longifolia$ Thuill., HF) and sheep fescue ($F.$ $ovina$ L., SF) were evaluated in the study. An alternative environmental condition requiring a FF germination test by International Seed Testing Association (ISTA) was applied in the experiment, consisting of 8-hr light at $25^{\circ}C$ and 16-hr dark at $15^{\circ}C$ (ISTA conditions). Daily and cumulative germination patterns were measured and analyzed on a daily basis. Significant differences were observed in germination pattern, days to the first germination, days to 50% germination, days to 60% germination, and germination rate. The final germination percentage was variable with species and varieties, being 40.25 to 82.00% at the end of study. There were considerable variations in early germination characteristics and germination pattern among FF species. The first germination in all entries except HF was initiated between 5 and 6 DAS (days after seeding) under ISTA conditions, while HF between 6 and 7 DAS, being 1 day later. It was 8 to 10 DAS in days to the 50% germination, which was 2 to 4 days after the first germination date. Days to the 60% germination were 9.10 to 14.80 DAS under ISTA conditions, being 5.70 days in differences among the entries. CF 'Jamestown II' and 'Shadow II' and HF 'Aurora Gold' were the fast varieties. The slowest one was HF 'Rescue 911'. Among FF species, turf establishment speed was becoming faster in CRF, SF, HF and CF in this order. Information on differences in germination characteristics and pattern from this study would be usefully applied for golf course design and construction, when established with FF.

A Study on the Transformational Christian Education for Young Adults: With a Focus on the Employment of Jung's Unconscious Confrontation and Loder's Transformational Theory (청년기의 기독교 변형화교육에 관한 연구: 융의 무의식 대면과 로더의 변형이론을 중심으로)

  • Lee, Kyoomin;Kim, Eunjoo
    • Journal of Christian Education in Korea
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    • v.63
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    • pp.121-150
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    • 2020
  • The important developmental tasks of young adults are based on encounter and identity. These problems of encounter and identity are also connected to the instinct of longing for the "face" of primary caregivers, who acknowledge and affirm themselves as their cherished children. James Loder emphasizes that human "face pursuit instinct" later reaches "formal-operational stage" and leads to religious yearning for God as "the Eternal Face." This pursuit of "face" and "the Eternal Face" is an existential and ontological move to find out "Who am I?" through meaningful encounters. Religious psychologist Carl Jung also points out that scientific thinking has contributed to the liberation of humans from superstitious beliefs. But this has also led to the loss of the precious value of human spirit and the sense of unity with nature. Jung emphasizes that "symbolic play" should help learners and counseler face-to-face with their unconscious mind. By doing so, learners can overcome the wounds and scars of unconsciousness and mature toward the true self. James Loder is a scholar who critically introduced Jung's "unconscientious confrontation" therapy to his educational theory. Beyond Jung's unconsciousness and "symbolic play," Loder proposed transformational education for the learners to participate in meaningful changes through interaction between human spirit and the Holy Spirit. With many young adults wandering around in their existential voids, it is clear that functional and socializational education cannot overcome their problems and developmental crisis. This developmental crisis requires a foundation of identity and intimacy in the encounter with God, the "Eternal Face." Therefore, this study suggests that when Jung's "unconscious confrontation" and Loder's "transformation logic" are employed, transformational Christian education for the healthy self-identity and intimacy of young adults can be accomplished. This inquiry presents not only theoretical reflection, but also the reactions of young adults and actual feedback obtained through implementing transformational Christian education for young adults. Through all of these endeavors, this inquiry was completed by proving that "Transformational Christian Education for Young Adults" is an educational theory that can yield actual results and abound fruits. (This enquiry was undertaken by the support of the research fund of PUTS 2020.)

Evaluation of Characteristics of Peanut Sprout Using Korean Cultivars (땅콩 품종을 이용한 싹나물 특성 평가)

  • Pae, Suk-Bok;Ha, Tae-Joung;Lee, Myoung-Hee;Hwang, Chung-Dong;Shim, Kang-Bo;Park, Chang-Hwan;Park, Keum-Yong;Baek, In-Yeol
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.56 no.4
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    • pp.394-399
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    • 2011
  • This experiment was conducted to select suitable cultivars and evaluate growth characteristics to get basic information for sprouting peanut. On sprouting peanut, it showed a rapid increase in trans-resveratrol content that has effects on anti-cancer, anti-inflammatory, blood-sugar-lowering and other beneficial cardiovascular in mouse. For this experiment, characteristics of peanut sprouts were tested in 37 cultivars grown for 7 days at $26^{\circ}C$ temperature. There were a lots of variations in the growth characteristics among cultivars as followers : The range of 100 grain weight was 56 to 142 g, hypocotyl length was 4.3 cm to 5.8 cm, diameter of hypocotyl was 5.0 to 8.0 mm, epicotyl length was 0.8 cm to 4.6 cm, seedling ratio per seed number was 84% to 100%, weight per seedling was 4.9 g to 8.4 g, the rate of hypocotyl cleavage was 0% to 46%, the content of trans-resveratrol was $22.5\;{\mu}g/g$ to $88.2\;{\mu}g/g$ and sprout yield was 360% to 820%. The selection points considered were high sprout yield, high seedling rate, high resveratrol content, low brownish cotyledon, no hypocotyl cleavage, and fat hypocotyl etc. The best cultivar selected was 'Jokwang' that showed 7.8 mm diameter, clean cotyledon color, 100% seedling rate, 0% hypocotyl cleavage, $63.3\;{\mu}g/g$ resveratrol, and 820% sprouting yield. This cultivar was expected to be of use as a new food and nutraceutical material. Relationship between growth characteristics showed that root length had significant positive correlations with epicotyl length, resveratrol content and sprouting yield but negative correlations with hypocotyl diameter and cleavage. Hundred grain weight showed negative correlations with resveratrol content, seedling rate and sprouting yield but positively correlated with curved hypocotyl rate and hypocotyl cleavage positively. This result showed small grain seed will be more appropriate for sprouting peanut.

Changes in Fatty Acid Composition and Phytosterol Content in Double Cropping Maize (이기작에 따른 옥수수의 지방산 조성 및 Phytosterol 함량 변화)

  • Kim, Sun-Lim;Jung, Gun-Ho;Kim, Mi-Jung;Son, Beom-Young;Kim, Jung-Tae;Bae, Hwan-Hee;Go, Young-Sam;Yi, Gibum;Lee, Jin-Seok;Baek, Seong-Bum
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.65 no.2
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    • pp.93-103
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    • 2020
  • The average growth day of 11 maize varieties from planting to silking in the first cropping (FC) was 89.5 days and in the second cropping (SC) was 46.7 days, which was 43 days faster than in the FC. The average 100-kernel weight (100 KW) in the FC was 28.4 g and 18.3 g in the SC, which was approximately 36.4% lower than that in the FC. The average crude oil content of FC was 3.97% and SC was 3.08%, which was about 0.89% lower than that of FC. The composition of stearic and oleic acid was significantly higher in FC, whereas palmitic and linoleic acid were higher in SC; however, linolenic acid was not statistically different between the two crops. The crude oil content was negatively correlated with linoleic acid (FC -0.264ns, SC -0.504**) and positively correlated with linolenic acid (0.526**). Unsaturated fatty acid (USFA) composition showed a significant difference between FC (83.48%) and SC (82.96%). Total phytosterol content was 598.3 mg/100 g and 701.9 mg/100 g in FC and SC, respectively, and showed significant difference by planting dates. The β-sitosterol content showed no statistical difference between the planting dates, but campesterol and stigmasterol were significantly higher in SC than in FC. Therefore, it was considered that the temperature condition during the ripening period affects the 100 KW of maize, and this leads to the variation in phytosterol content. However, among phytosterols, β-sitosterol was relatively little affected by the planting dates. USFA composition showed a significantly negative correlation with phytosterol content. Considering the results, the relatively high phytosterol content in SC was mainly because of the delay in progress of starch accumulation as daily air temperature decreased from the middle of the ripening period, whereas progress of phytosterol accumulation in the maize kernel was considered relatively faster than that of starch accumulation.

The 'Fantastic' in the René Laloux's movie (<죽은 시간들(Les Temps Morts), 르네 랄루(René Laloux) 작, 1964>의 환상성)

  • Han, Sang-Jung;Park, Sang-Chun
    • Cartoon and Animation Studies
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    • s.27
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    • pp.31-49
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    • 2012
  • This research aims at showing specificity of the 'fantastic' in the movie < Les temps morts (on 1964) > directed by Ren$\acute{e}$ Laloux (1929-2004 ), all over the world recognized director. This movie has a particular style by composing four forms of expression: the real recording (movie), the recording embellishes with images by image (animation), the drawing, the photo. This film is the most strange among his all films. Even if we could catch the key meaning of the film, it offer for the audience the sentiment incertain and unclear. If we consider 'the fantastic' as a hesitation between the real and the unreal, in diverse levels, this movie offers to the spectators the fantastic feelings. In order to present the way this film shows us the fantastic, we divide the film into 15 sequences according to the criteria of the visual elements and the auditive elements. We analyze specificities of this fantastic in diverse levels. At first, the first style of the drawing of Roland Topor, does not let us escape easily from the feeling of fantasy. The four representation formats(drawing, photo, animation, movie) are integrated into one whole by auditive elements(music, narration). On the other hand, certain parts incomprehensible are not integrated into the entire. are fully integrated into the unity that does not understand that part, leaving them can. Laloux leads the audience into a reality toward the end of the film, but he leave incertain sequences at the last moment. Through which the audience is again hesitant between the real and the unreal, the fantastic is strengthened as a result of the work. Finally, the fantastic of the film could be found at three levels. First, the fantastic drawing style of Roland Topor. In the second place, the fantastic exposed through the entire composition and structure of work. Overall, these by leaving through the availability of the story incomprehensible to the audience is to provide a fantastic sentiment.

Anti-Inflammatory Effect of Wheat Germ Oil on Lipopolysaccharide-stimulated RAW 264.7 Cells and Mouse Ear Edema (LPS로 유도한 RAW 264.7 세포 및 귀부종 동물 모델에 대한 밀배아유의 항염증 효과)

  • Kang, Bo-Kyeong;Kim, Min-Ji;Jeong, Da-Hyun;Kim, Koth-Bong-Woo-Ri;Bae, Nan-Young;Park, Ji-Hye;Park, Sun-Hee;Ahn, Dong-Hyun
    • Microbiology and Biotechnology Letters
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    • v.44 no.3
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    • pp.236-245
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    • 2016
  • This study investigated the anti-inflammatory effects of wheat germ oil (WGO) on RAW 264.7 cells. It was shown that WGO had no cytotoxicity against the treated cells or negative effect on their proliferation. WGO suppressed nitric oxide (NO) secretion considerably and had inhibitory effects on the production of LPS-induced NO and pro-inflammatory cytokines (IL-6, TNF-α, and IL-1β). In particular, the IL-6 and TNF-α inhibition activities were over 90% at 100 μg/ml concentration of the oil. WGO also inhibited the LPS-induced expression of cyclooxygenase-2, inducible nitric oxide synthase, and nuclear factor-kappa B (NF-κB), and reduced the expression of phosphorylated ERK and JNK. Moreover, the croton-oil-induced edema in mouse ears was reduced by WGO, and no mortalities occurred in mice administered 5,000 mg/kg body weight of WGO over a 2-week observation period. In conclusion, these results provide evidence for the anti-inflammatory effect of WGO that likely occurs via modulation of NF-κB and the JNK/ERK MAPK signaling pathway.

Evaluation of Airborne Pb Sources in an Industrialized City by Applying Pb Isotope Ratios and Concentrations in PM10 (PM10 내 납의 동위원소와 농도를 활용한 산업도시지역 대기 중 납 오염원 평가)

  • Jo, Wan-Kuen;Lee, Heon-Chul;Kim, Mo-Keun
    • Journal of Korean Society of Environmental Engineers
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    • v.33 no.3
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    • pp.174-182
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    • 2011
  • The present study evaluated the major lead sources in a steel metallurgy industrialized city by measuring lead isotopes/lead concentrations of ambient air and potential sources in an industrial area and residential areas according to relative distance. The quality control program obtained during the measurement procedure for lead isotopes and concentrations exhibited $0.5ng/m^3$ for method detection limit, more than 90% for recoveries of standard particulate matters, and lower than 0.2% for reproducibility errors of four lead isotopes ($^{204}Pb$, $^{206}Pb$, $^{207}Pb$, $^{208}Pb$). For all three lead isotope ratios ($^{206}Pb/^{204}Pb$, $^{207}Pb/^{206}Pb$, $^{208}Pb/^{206}Pb$), the ratios were obtained in the industrial area were closer to nearby residential area than those of a residential area far away from the industrial area, thereby suggesting that lead sources were more similar each other in the industrial and nearby residential area. Furthermore, for both summer and winter seasons ambient lead concentrations were more than four times higher in the industrial area than in the residential areas and in turn, they were higher in the nearby residential area compared with the far-away residential area. As a result, it was suggested that lead emitted from the industrial area would influence more the ambient lead in the nearby residential area than the far-away residential area. Both slag and traffic emissions are likely to be major lead sources in the industrial and nearby residential areas, since their three lead isotope ratios ($^{206}Pb/^{204}Pb$, $^{207}Pb/^{206}Pb$, $^{208}Pb/^{206}Pb$) were similar to the ratios obtained from ambient air of these two areas. In addition, the lead isotope ratios revealed different pattern between seasons, and the ambient lead concentrations were higher for winter than for summer.

Whitening and anti-wrinkle effect of Pinus koraiensis leaves extracts according to the drying technique (건조방법에 따른 잣나무(Pinus koraiensis) 잎 추출물의 주름개선 및 미백 효과)

  • Jo, Jae-Bum;Park, Hye-Jin;Lee, Eun-Ho;Lee, Jae-Eun;Lim, Su-Bin;Hong, Shin-Hyub;Cho, Young-Je
    • Journal of Applied Biological Chemistry
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    • v.60 no.1
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    • pp.73-78
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    • 2017
  • This study provide activity for beauty food of water and 80 % ethanol extracts from Pinus koraiensis leaves. Total phenolic content of extracts from Pinus koraiensis leaves were each 12.22 mg/g (Drying under hot air) and 17.93 mg/g (Drying under shade), 14.36 mg/g (Lyophilization) in water extracts (WE) and 11.9 mg/g and 20.63 mg/g, 17.96 mg/g in 80 % ethanol extracts (EE). The 1,1-diphenyl-2-picrylhydrazyl free radical scavenging activity of extracts from Pinus koraiensis leaves was 96.20 % in EE from drying under shade at extracts concentration. The 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical decolorization activity of extracts from drying under shade was 99.85 % in WE and 99.80 % in EE at extracts concentration. The antioxidant protection factor (PF) extracts from drying under shade type was 9.63 PF in WE and 10.48 PF in EE at extracts concentration. The thiobarbituric acid reactive substance from Pinus koraiensis leaf was 89.39 % in EE from drying under shade at extracts concentration. The elastase inhibition activity of EE for anti-wrinkle effect showed an excellent wrinkle improvement effect, showing 71.46 % in EE from lyophilization. Collagenase inhibition activity of EE from drying under shade was 97.48 % in extracts. Tyrosinase inhibition activity which was related to anti-melanogensis was observed. The tyrosinase inhibitory effect of extracts from lyophilization was confirmed to be 60.4 % in EE more than another drying methods at extracts concentration. Through out all results, it can be expected Pinus koraiensis leaves extracts to use as a functional material for anti-oxidant and functional beauty food.

Anti-inflammatory effect potentials of ethanol extracts from fermentated Caryopteris incana by Lactobacillus plantarum on induced to LPS with Raw 264.7 cell (LPS로 유도된 Raw 264.7 cell에서 Lactobacillus plantarum 발효가 층꽃나무(Caryopteris incana) 에탄올 추출물의 염증반응에 미치는 영향)

  • Park, Mi-Jeong;Park, Hye-Jin;Lee, Eun-Ho;Jung, Hee-Young;Cho, Young-Je
    • Journal of Applied Biological Chemistry
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    • v.61 no.2
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    • pp.141-150
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    • 2018
  • In this study, the inflammation of ethanol extracts from Caryopteris incana (CI) and fermented C. incana (FCI) on induced to lipopolysaccharide with Raw 264.7 cell was tested. The composition profile of L. plantarum was changed by fermentation, and confirmed by HPLC analysis. We performed the 3-[4,5-dimethylthiazol]-2-yl]-2,5-diphenyltetrazolium bromide assay to evaluate the toxicity of CI and FCI extracts. In cell viability, cell toxicity was not shown at 5, 10 and $15{\mu}g/mL$ of CI extracts and 10, 20, 30 and $40{\mu}g/mL$ of FCI extracts. The results of inducible nitric oxide synthase and cyclooxygenase-2 protein production were confirmed to be inhibitory in a concentration-dependent manner, respectively. Additionally, protein expression of nitric oxide and prostaglandin $E_2$ by CI and FCI extracts were also inhibited in a concentration-dependent manner. In the result of pro-inflammatory cytokine, $15{\mu}g/mL$ concentration of CI extracts was showed tumar necrosis factor $(TNF)-{\alpha}$ (57.3%), interleukin (IL)-6 (35.2%), and $IL-1{\beta}$ (48.0%), respectively. And $40{\mu}g/mL$ of FCI extracts was showed $TNF-{\alpha}$ (34.6%), IL-6 (32.1%), and $IL-1{\beta}$ (30.0%), respectively. These results suggest that FCI extracts showed better effect of anti-inflammatory than CI extracts. Therefore, it was found that both CI and FCI can be used as an excellent material for the development of new anti-inflammatory resource.

On the Secretion and Functions of Equine Chorionic Gonadotropin (말의 융모성 성선자극 호르몬의 분비와 기능)

  • Min, K.S.
    • Korean Journal of Animal Reproduction
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    • v.24 no.2
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    • pp.125-142
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    • 2000
  • 13). Analysis of a purified preparation of eCG revealed that its $\beta$ -subunit consists of 149 amino acids, which was confirmed by the molecular cloning of its cDNA. There seem to be at least four to six, or even as many as 11, O-glycosylation sites on the extended C-tenninal region of the eCG $\beta$-subunit. Interestingly, eCG is a unique member of this family, as it appear to be a single molecule that possesses both LH- and FSH-like activities. Using the cDNA prepared from mRNA extracted from equine placental and pituitary tissues, we cloned the cDNA of eCG $\alpha$- and $\beta$ -subunits and eFSH $\beta$ -subunit. The mRNA expression of each subunit seems to be independently regulated, which may account for differences in the quantities of $\alpha$ - and $\beta$ -subunits in the placenta and pituitary. Thus, eCG is a distinct molecule from the view points of its biological function and glycoresidue structures. Recombinant eCGs including the mutants which lack oligosaccharides will be useful tools for analyzing the structure-function relationships of gonadotropins in the horse as well as other species. Similar experiments will also clarify the proposed structure and biological functions for the glycoprotein hormones. These experimental are now possible, and hopefully a resolution of the existing controversy will be forthcoming in the near future.

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