• Title/Summary/Keyword: IOTA

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Changes of Nutrient Content of Circulating Solution in Three Different New Hydroponics for Oriental Melons(Cucumis melo L. var. makuwa Mak.) (참외용 수경재배시스템에서의 순환배양액의 무기이온 함량의 변화)

  • 조익환;전하준
    • Journal of Bio-Environment Control
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    • v.11 no.4
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    • pp.168-174
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    • 2002
  • A new circulating hydroponic system was invented for oriental melons grown in the greenhouse. For developing nutrient solution management techniques, we examined the changes of nutrient contents of circulating solution in three different types of new hydroponic systems. The yield and fruit quality of oriental melons in Hydroponics were better than those in soil culture. The substrate culture was appropriate fer hydroponics of oriental melons, and NFT was turned to be the opposite due to the physiological disorder during hot seasons. Yamazaki's melon solution with EC 2.0dS.m$^{[-10]}$ was the most appropriate for oriental melons. The new circulating hydroponic system seemed to be appropriate for oriental melons because of the stable EC, pH and the macro- and micro-element contents. NO$_3$-N, Ca and Mg contents in the circulating solution kept a good balance in all types of hydroponics. However, p content, compared to other types, decreased by the degree of 1 me.L$^{[-10]}$ in perlite medium. K content showed irregular status in perlite but showed the stable status in cocopeat. Generally, microelements, except Mo, showed stable absorption in the substrate culture. However, in NET, most of the elements showed irregular absorption except B and Mn. Microelement absorption, especially Cu, Zn and Mo, decreased during hot seasons.

The roles of PKC-δ on the regulation of insulin-like growth factor(IGF)-I and insulin-Like growth factor binding protein-3 secretion by all-trans retinoic acid in MCF-7 cell (MCF-7 cell에서 all-trans retinoic acid에 의한 insulin-like growth factor-I와 insulin-like growth factor binding protein-3 분비조절에 있어서 PKC-δ의 역할)

  • Lee, Sun-Mi;Kim, Sang-Hoon;Choi, Kwang-Soo;Kang, Chang-Won
    • Korean Journal of Veterinary Research
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    • v.46 no.2
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    • pp.97-105
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    • 2006
  • All-trans retinoic acid (AtRA) induces growth inhibition and apoptosis in a variety of tumer cells, including MCF-7 cells. Insulin-like growth factors (IGFs) system has been reported to be associated with the development of cancer. Although MCF-7 cell with AtRA is to be the major stimulus for the cell growth and apoptosis, the mechanism of insulin-like growth factor-I (IGF-I)/insulin-like growth factor binding protein-3 (IGFBP-3) system remains to be elucidated. Thus, this study was conducted to the effect of AtRA on the gene expression and level of IGF-I and IGFBP-3. In addition, we investigated the involvement of PKC-${\delta}$ on the IGF-I and IGFBP-3 secretion in MCF-7 cell. AtRA(${\geq}10^{-7}M$) decreased the IGF-1 secretion and mRNA expressions, but increased IGFBP-3 secretion and mRNA expressions in MCF-7 cells. Especially, the treatment of AtRA at 72 hours caused a significant reduction in the IGF-I secretion and mRNA expressions but increment in IGFBP-3 secretion and mRNA expressions (p < 0.05). $10^{-7}M$ AtRA activated PKC-${\delta}$ that is one among PKC-$\iota$, ${\alpha}$, ${\lambda}$ and ${\delta}$ in MCF-7 cell. Rotllerin, a PKC-${\delta}$ inhibitor, blocked AtRA-induced inhibition of the IGF-I and mRNA expressions, and increase of lGFBP-3 and mRNA expressions in MCF-7 cell. Together, AtRA inhibited the IGF-I secretion and mRNA expressions, but increased IGFBP-3 secretion and mRNA expressions in MCF-7 cell. Furthermore, AtRA-induced alteration of IGF-I, IGFBP-3 secretion, and the gene expressions were mediated via PKC-${\delta}$ activity.

Mycobacterium tuberculosis-induced Expression of Interleukin-1 Beta is Mediated Via Protein Kinase C Signaling Pathway

  • Cho, Jang-Eun;Lee, Kyung-Hong;Son, Sin-Jee;Park, Sang-Jung;Lee, Hye-Young;Kim, Yoon-Suk
    • Biomedical Science Letters
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    • v.16 no.2
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    • pp.119-122
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    • 2010
  • Interleukin-1${\beta}$ $(IL-1{\beta})$ is one of the key proinflammatory cytokines and it plays an important role for the antimycobacterial host defense mechanisms. In this study, we examined Mycobacterium tuberculosis (MTB)-stimulated induction of IL-1${\beta}$ and evaluated the associated signal transduction pathways. In PMA-differentiated THP-1 cells, MTB infection increased mRNA expression of IL-$1{\beta}$ in a dose-dependent manner. The expression of IL-1${\beta}$ mRNA began to be induced at 1.5 h after infection, and induced expression of IL-1${\beta}$ was retained for 48 h after MTB infection. The increase in expression of IL-1${\beta}$ caused by MTB was reduced in cells treated with Ro-31-8425 (an inhibitor of PK$C{\alpha}$, ${\beta}I$, ${\beta}II$, ${\gamma}$, ${\varepsilon}$) or PD98059 (an inhibitor of MEK1), meanwhile, pre-treatment with $G\ddot{o}6976$ (an inhibitor of $Ca^{2+}$ dependent PK$C{\alpha}$ and PK$C{\beta}I$) or Rottlerin (an inhibitor of PK$C{\delta}$) has no effect on MTB-induced expression of $IL-1{\beta}$ mRNA. These results show that the expression of $IL-1{\beta}$ mRNA caused by MTB may be mediated via MEK1 and PKC isoforms including PK$C{\beta}II$, $PKC{\gamma}$, or $PKC{\varepsilon}$. Further studies are required to determine whether other PKC isoforms $(PKC {\eta},\;{\theta},\;{\varepsilon},\;and\;{\lambda}/{\iota})$, except $PKC{\delta}$, $PKC{\alpha}$, and $PKC{\beta}I$, are also involved in $IL-1{\beta}$ mRNA expression after mycobacterial infection.

Evaluation of Yield Load in Pile Load Tests on Driven Piles (관입말뚝에 대한 연직재하시험시 항복하중의 판정법)

  • 홍원표;심기석
    • Geotechnical Engineering
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    • v.5 no.1
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    • pp.7-18
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    • 1989
  • In pile load tests on end bearing piles, generally, it is not possible to continue loading to the ultimate load. Thus, the concept of yield load has been introduced for determining design loads Iron the pile load test records. The conventional rules to determine the yield load were not available for evaluation on pile load test records obtained in 6 fields nearby westers 8r Southern Coasts in Korea. A new rule 9.as presented to determine easily the yield load, based on investigations on the pile load test records. The yield load of piles is determined at the infiection point on semi-logarithmic coordinates (P-logS), in which load is plotted in normal scale and settlement is plotted in logarithmic scale. This method may not only save much costs and times but also present safe luorking circumstances for pile load tests in field. It was found that the yield load represented the elastic limit of the pile load-settlement behalf.iota. The ultimate load, which is given at 25.4mm settlement on pile head, was 1.5 times of the yield load. The allowable long-term and short-term load capacities were, respectively, 50% and 75% of the yield load. The safety factors to get the allowable pile capacity were obtained as 2.0~4.0 for the equations to predict the static pile capacity.

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Volume of Water Storage and Evapotranspiration by Inserted Materials at a Reservoir of Porous Grass Block (저수형 잔디블록 저수조 내 충진재료에 따른 저수량 및 초종별 증발산량)

  • Han, Seung-Ho;Choi, Joon-Soo;Yang, Geun-Mo;Yang, Byoung-E;Kang, Jin-Hyoung;Kim, Won-Tae
    • Journal of the Korean Institute of Landscape Architecture
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    • v.34 no.5 s.118
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    • pp.76-83
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    • 2006
  • The purpose of this study was to investigate the performance of porous grass block. For the investigation, Festuca arundinacea and Zoysia japonica 'Zenith' were planted, and the volume of evapotranspiration and remains were examined based on different materials in the water tank in the experiment of Festuca arundinacea, the volume of water storage of treatment with perlite ($10.84{\iota}/m^2$) was higher than that with drainage ($7l/m^2$). The difference between the two was $3.84/m^2$. The drainage treatment without water storage capacity showed the higher degree of dryness in turf grass. The volume of evapo-transpiration of treatment with perlite was the highest (21.57mm/week). The volume of evapotranspiration of treatment with sand was 19.57mm/week, and with treatment with drainage was 18.24mm/week. Based on the measured volume of daily evapotranspiration of $2.60{\sim}3.08mm\;d^{-1}$, it was determined that the unit with water storage capacity would store water of one to two days usage compared to unite without such storage capacity. In the experiment of Zoysia japonica 'Zenith', the volume of water storage of treatment with perlite was $10.77l/m^2$ which was similar to the former experiment. The volume of evapotranspiration of treatment with perlite and sand were 21.64mm/week and 20.64mm/week, respectively. In case of airtight water tank, the volume was measured as 22.06mm/week. Each treatment has no notable difference in the volume of evapotranspiration. In conclusion, from the investigation in this study, porous grass block with water tank was found to be effective in plant growth under low irrigation. As the ecological area ratio and vegetated porous pavement have became more emphasized, additional study of rain infiltration and reservoir effect are needed in the future.

A Study on the Vented Gas Explosion Characteristics of Indoor Leakage of the LPG (실내 LPG 누출시 폭발특성에 관한 연구)

  • Oh Kyu-hyung;Kim Hong;Kim Sang-sub;Jo Yoong-do;Jo Jee-whan;Oh Shin-kyu
    • Journal of the Korean Institute of Gas
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    • v.3 no.3 s.8
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    • pp.51-57
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    • 1999
  • A study on the vented gas explosion characteristics were carried out with the liquified petroleum gas(LPG) which is used in domestics and industries fuel. To evaluate a damage by gas explosion and to predict a explosion hazards, a series of experiment have been performed in the regular hexahedron vessel of 270${\iota}$. A side of the vessel was made to setting a polyester diaphragm which was ruptured by explosion to simulate an accidental explosion which ruptured the window by explosion. Experimental parameters were LPG concentration, ignition position, venting area, a strength of diaphragm which was ruptured and distances from venting, Experimental results showed that vented gas explosion pressure was more affected by the diaphragm strength than the gas concentration, and the vented gas explosion pressure and blast wave pressure was increased with decreasing the venting area and increasing the strength of diaphragm. In this research we can find that a damage by vented explosion at the outside can be larger than the inside by blast wave pressure near the venting.

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Error-Prone and Error-Free Translesion DNA Synthesis over Site-Specifically Created DNA Adducts of Aryl Hydrocarbons (3-Nitrobenzanthrone and 4-Aminobiphenyl)

  • Yagi, kashi;Fujikawa, Yoshihiro;Sawai, Tomoko;Takamura-Enya, Takeji;Ito-Harashima, Sayoko;Kawanishi, Masanobu
    • Toxicological Research
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    • v.33 no.4
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    • pp.265-272
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    • 2017
  • Aryl hydrocarbons such as 3-nitrobenzanthrone (NBA), 4-aminobiphenyl (ABP), acetylaminofluorene (AAF), benzo(a)pyrene (BaP), and 1-nitropyrene (NP) form bulky DNA adducts when absorbed by mammalian cells. These chemicals are metabolically activated to reactive forms in mammalian cells and preferentially get attached covalently to the $N^2$ or C8 positions of guanine or the $N^6$ position of adenine. The proportion of $N^2$ and C8 guanine adducts in DNA differs among chemicals. Although these adducts block DNA replication, cells have a mechanism allowing to continue replication by bypassing these adducts: translesion DNA synthesis (TLS). TLS is performed by translesion DNA polymerases-Pol ${\eta}$, ${\kappa}$, ${\iota}$, and ${\zeta}$ and Rev1-in an error-free or error-prone manner. Regarding the NBA adducts, namely, 2-(2'-deoxyguanosin-$N^2$-yl)-3-aminobenzanthrone (dG-$N^2$-ABA) and N-(2'-deoxyguanosin-8-yl)-3-aminobenzanthrone (dG-C8-ABA), dG-$N^2$-ABA is produced more often than dG-C8-ABA, whereas dG-C8-ABA blocks DNA replication more strongly than dG-$N^2$-ABA. dG-$N^2$-ABA allows for a less error-prone bypass than dG-C8-ABA does. Pol ${\eta}$ and ${\kappa}$ are stronger contributors to TLS over dG-C8-ABA, and Pol ${\kappa}$ bypasses dG-C8-ABA in an error-prone manner. TLS efficiency and error-proneness are affected by the sequences surrounding the adduct, as demonstrated in our previous study on an ABP adduct, N-(2'-deoxyguanosine-8-yl)-4-aminobiphenyl (dG-C8-ABP). Elucidation of the general mechanisms determining efficiency, error-proneness, and the polymerases involved in TLS over various adducts is the next step in the research on TLS. These TLS studies will clarify the mechanisms underlying aryl hydrocarbon mutagenesis and carcinogenesis in more detail.

Removal Efficiency of Cochiodinium polykrikoides by Yellow Loess (황토의 유해성 적조생물 Cochiodinium종의 제거효과)

  • CHOI Hee Gu;KIM Pyoung Soong;LEE Won Chan;YUN Seong Jong;KIM Hak Gyoon;LEE Hung Jae
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.31 no.1
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    • pp.109-113
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    • 1998
  • The clay and yellow loess have capability to adsorb and precipitate particles. The removal efficiencyes of those flocculents on the dinoflagellate, Cochlodinium polykrikoides, have been studied in laboratory and in field near Tongyong fish farm in September, 1996. The removal efficiencyes in the laboratory experiment was $43\%$ for $2\;g/{\ell}$, $64\%$ for $6g/{\ell}$ and $88\%$ for $10\;g/{\ell}$ in one hour after dispersion. No big difference of removal efficiency was found between the raw and the acid-activated loess. In the field survey, the removal rates ranged from 72 to $80\%$ in 30 min after the dispersion. The effect of loess scattering on water quality was estimated. The concentrations of dissolved inorganic nitrogen (DIN), chemical of gen demand (COD) and chlorophyll a decreased more or less after dispersion, while the concentration of suspended solid (SS) increased. The concentrations of dissolved oxygen (DO) and dissolved inorganic phosphorous (DIP) were kept constant. These results indicated that the dispersion concentration of more than $10g/{\ell}$ has a good removal efficiency of above $80\%$ without big variation of water quality after dispersion of yellow loess.

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Impact of Tofu Paste and Non-starch Polysaccharides on Oil Uptake Reduction in Cake Doughnuts (케이크 도넛의 흡유저감에 대한 두부 페이스트와 비전분성 탄수화물 고분자의 영향)

  • Jung, Gil-Young;Lee, Hyeon-Jeong;Ko, Eun-Sol;Kim, Hyun-Seok
    • Food Engineering Progress
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    • v.21 no.1
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    • pp.72-78
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    • 2017
  • The objective of this study was to investigate the effects of combinations of tofu paste and non-starch polysaccharides (NSP) on the oil uptake reduction (OTR) of deep-fat fried cake doughnuts. OTR agents were tofu paste (from grinding tofu with deionized water, followed by passage through a 60 mesh sieve), and five neutral and nine anionic NSPs. A control doughnut (without tofu paste or NSP), tofu doughnut (with tofu paste) and NSP-tofu doughnut (with tofu paste and NSP) were prepared. The moisture and total lipid (TL) content, cross-section image, color characteristic, and specific volume were measured. The tofu and NSP-tofu doughnuts exhibited higher moisture and lower TL content than the control. OTR was 10.8% for the tofu doughnut, and between 13.2% and 41.2% for the NSP-tofu doughnut. The highest OTR (41.2%) was found in the NSP-tofu doughnut with a combination of tofu paste and sodium alginate (NaA). The specific volume of the NSP-tofu doughnuts with combinations of tofu paste with NaA (2.5 mL/g), locust bean gum (2.5 mL/g), and ${\kappa}$-carrageenan (2.4 mL/g) was very close to that of the control (2.6 mL/g). Considering the OTR and specific volume of doughnuts, the combination of tofu paste and NaA would be most effective in reducing the oil uptake of doughnuts during deep-fat frying.

Privacy Preserving Data Publication of Dynamic Datasets (프라이버시를 보호하는 동적 데이터의 재배포 기법)

  • Lee, Joo-Chang;Ahn, Sung-Joon;Won, Dong-Ho;Kim, Ung-Mo
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.18 no.6A
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    • pp.139-149
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    • 2008
  • The amount of personal information collected by organizations and government agencies is continuously increasing. When a data collector publishes personal information for research and other purposes, individuals' sensitive information should not be revealed. On the other hand, published data is also required to provide accurate statistical information for analysis. k-Anonymity and ${\iota}$-diversity models are popular approaches for privacy preserving data publication. However, they are limited to static data release. After a dataset is updated with insertions and deletions, a data collector cannot safely release up-to-date information. Recently, the m-invariance model has been proposed to support re-publication of dynamic datasets. However, the m-invariant generalization can cause high information loss. In addition, if the adversary already obtained sensitive values of some individuals before accessing released information, the m-invariance leads to severe privacy disclosure. In this paper, we propose a novel technique for safely releasing dynamic datasets. The proposed technique offers a simple and effective method for handling inserted and deleted records without generalization. It also gives equivalent degree of privacy preservation to the m-invariance model.