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Flavonoids as Substrates of Bacillus halodurans O-Methyltransferase

  • Jeong, Ki-Woong;Lee, Jee-Young;Kang, Dong-Il;Lee, Ju-Un;Hwang, Yong-Sic;Kim, Yang-Mee
    • Bulletin of the Korean Chemical Society
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    • v.29 no.7
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    • pp.1311-1314
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    • 2008
  • Bacillus halodurans O-methyltransferase (BhOMT) is an S-adenosylmethionine dependent methyltransferase. In our previous study, three dimensional structure of the BhOMT has been determined by comparative homology modeling and automated docking study showed that two hydroxyl groups at 3'- and 4'-position in Bring and structural rigidity of C-ring resulting from the double bond characters between C2 and C3 of flavonoid, were key factors for interaction with BhOMT. In the present study, BhOMT was cloned and expressed. Binding assay was performed on purified BhOMT using fluorescence experiments and binding affinity of luteolin, quercetin, fisetin, and myricetin were measured in the range of $10^7$. Fluorescence quenching experiments indicated that divalent cation plays a critical role on the metal-mediated electrostatic interactions between flavonoid and substrate binding site of BhOMT. Fluorescence study confirmed successfully the data obtained from the docking study and these results imply that hydroxyl group at 7-position of luteolin, quercetin, fisetin, and myricetin forms a stable hydrogen bonding with K211 and carboxyl oxygen of C-ring forms a stable hydrogen bonding with R170. Hydroxyl group at 3'-and 4'-position in the B-ring also has strong $Ca^{2+}$ mediated electrostatic interactions with BhOMT.

Processing, structure, and properties of lead-free piezoelectric NBT-BT

  • Mhin, Sungwook;Lee, Jung-Il;Ryu, Jeong Ho
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.25 no.4
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    • pp.160-165
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    • 2015
  • Lead-free piezoelectric materials have been actively studied to substitute for conventional PZT based solid solution, $Pb(Zr_xTi_{1-x}O_3)$, which occurs unavoidable PbO during the sintering process. Among them, Bismuth Sodium Titanate, $Na_{0.5}Bi_{0.5}TiO_3$ (abbreviated as NBT) based solid solution is attracted for the one of excellent candidates which shows the strong ferroelectricity, Curie temperature (Tc), remnant polarization (Pr) and coercive field (Ec). Especially, the solid solution of rhombohedral phase NBT with tetragonal perovskite phase has a rhombohedral - tetragonal morphotropic phase boundary. Modified NBT with tetragonal perovskite at the region of MPB can be applied for high frequency ultrasonic application because of not only its low permittivity, high electrocoupling factor and high mechanical strength, but also effective piezoelectric activity by poling. In this study, solid state ceramic processing of NBT and modified NBT, $(Na_{0.5}Bi_{0.5})_{0.93}Ba_{0.7}TiO_3$ (abbreviated as NBT-7BT), at the region of MPB using 7 % $BaTiO_3$ as a tetragonal perovskite was introduced and the structure between NBT and NBT-7BT were analyzed using rietveld refinement. Also, the ferroelectric and piezoelectric properties of NBT-7BT such as permittivity, piezoelectric constant, polarization hysteresis and strain hysteresis loop were compared with those of pure NBT.

Magnetic Properties of NiZn-ferrite Synthesized from Waste Iron Oxide Catalyst (산화철 폐촉매로부터 합성된 NiZn- 페라이트의 자기적 특성)

  • Hwang, Yeon;Kwon, Soon-Kil;Lee, Hyo-Sook;Je, Hae-June;Park, Sang-Il
    • Journal of the Korean Ceramic Society
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    • v.38 no.12
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    • pp.1162-1166
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    • 2001
  • NiZn-ferrite was synthesized from waste catalysts, which were produced from styrene monomer process and buried underground as an industrial wastes, and its magnetic properties were investigated. Nickel oxide and zinc oxide powders were mixed with finely ground waste catalysts, and spinel type ferrite was obtained by calcination at 900$\^{C}$ and sintering at 1230$\^{C}$ for 5 hours. The intial permeability was measured and reflection loss was calculated from S-parameters for the composition of Ni$\_$x/Zn$\_$1-x/Fe$_2$O$_4$(x=0.36, 0.50, 0.66). NiZn-ferrite synthesized from waste iron oxide catalyst showed a feasibility for the use as electromagnetic wave absorber in X-band.

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Lossless Data Hiding Using Modification of Histogram in Wavelet Domain (웨이블릿 영역에서 히스토그램 수정을 이용한 무손실 정보은닉)

  • Jeong Cheol-Ho;Eom Il-Kyu;Kim Yoo-Shin
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.43 no.4 s.310
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    • pp.27-36
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    • 2006
  • Lossless data embedding is a method to insert information into a host image that guarantees complete restoration when the extraction has been done. In this paper, we propose a noble reversible data embedding algorithm for images in wavelet domain. The proposed embedding technique, which modifies histogram of wavelet coefficient, is composed of two inserting steps. Data is embedded to wavelet coefficient using modification of histogram in first embedding process. Second embedding step compensates the distortion caused by the first embedding process as well as hides more information. Hence we achieve higher inserting capacity. In view of the relationship between the embedding capacity and the PSNR value, our proposed method shows considerably higher performance than the current reversible data embedding methods.

Dielectric and Piezoelectric Characteristics of Low Temperature Sintering 0.20Pb(Zn1/3Nb2/3)O3-0.80Pb(Zr0.48Ti0.52)O3 Ceramics with the Addition of Sintering Aid ZnO (소결조제 ZnO 첨가에 따른 저온소결 0.20Pb(Zn1/3Nb2/3)O3-0.80Pb(Zr0.48Ti0.52)O3 세라믹스의 유전 및 압전특성)

  • Yoo, Ju-Hyun;Lee, Yu-Hyong;Kim, Do-Hyung;Lee, Il-Ha;Kwon, Jun-Sik;Paik, Dong-Soo
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.21 no.2
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    • pp.126-130
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    • 2008
  • In this study, in order to develop low loss multilayer piezoelectric actuator, PZN-PZT ceramics were fabricated using $Li_2CO_3,\;Bi_2O_3$, CuO and ZnO as sintering aids, their structural, piezoelectric and dielectric characteristics were investigated according to the amount of ZnO addition, At the sintering temperature of $870^{\circ}C$, the density, electromechanical coupling factor(kp), mechanical quality factor(Qm), dielectric constant(${\epsilon}_r$) and piezoelectric constant($d_{33}$) of 0.4 wt% ZnO added specimen (sintered at $870^{\circ}C$) showed the optimum value of $7.812g/cm^3$, 0.535, 916, 1399, 335 pC/N respectively. Taking into consideration above piezoelectric properties of the specimen sintered at low temperature, it was concluded that PZN-PZT ceramics using 0.4 wt% ZnO as additive showed the optimum characteristics as the composition ceramics for low loss multilayer piezoelectric actuator application.

Inhibition Effect of ACE (Angiotensin Converting Enzyme) and Kinetics of Aloe Acethylmannan (알로에 아세칠만난의 ACE (Angiotensin Converting Enzyme) 저해효과 및 동력학적 분석)

  • Ryu, Il-Whan;Shin, Yong-Seo
    • Korean Journal of Food Science and Technology
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    • v.29 no.6
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    • pp.1269-1274
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    • 1997
  • This study was researched to purify and characterize variety bioactive material acethylmannan from Aloe vera. Purified acethylmannan was mannose (67%), acetyl group (23%) and the rest glucose, galactose that consisting of long chain polydispered ${\beta}-1,4$ linked mannan polymers. The sugar and acetyl group in molecular were linked molar ration one third. $IC_{50}$ value (i.e that concentration which exhibits 50% more enzyme inhibition than control) on angiotensin converting enzyme were 0.58 mM. This compound were found to be a competitive inhibition of Angiotensin Converting Enzyme with apparent Ki values of 0.068 mM.

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Strategy of Driver Selection in C3MR Process Considering Extraction Rate from Natural Gas Well (가스전의 추출속도를 고려한 C3MR 공정의 동력기 선택전략)

  • Lee, Sunkyu;Lee, Inkyu;Tak, Kyungjae;Moon, Il
    • Journal of the Korean Institute of Gas
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    • v.20 no.1
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    • pp.7-12
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    • 2016
  • Natural gas liquefaction process is essential to transport natural gas for long distances. Lots of compressors in this process are needed and the energy for these compressors can be supplied by drivers. Total driver cost can be changed by selecting various drivers. This study focused on the minimization of the driver cost to provide the energy to the compressors. Moreover, scenarios, extracting velocity is changed during whole operating period, are set with considering gas well capacity. The mathematical model was established by considering trade off relationship between the capital cost and the operating cost of the turbines. The model also considers the life time of the driver equipments. As the result, the driver cost of the optimized case was reduced by 6.4% than the base case.

Optimum Conditions for the Separation of Lecithin from Egg Yolk by Response Surface Methodology (반응표면 분석법을 이용한 계란 난황 레시틴 분리 최적조건)

  • Jang, Ae-Ra;Lim, Dong-Gyun;Jeon, Hee-Joon;Jo, Cheo-Run;Kim, Il-Joon;Lee, Moo-Ha
    • Food Science of Animal Resources
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    • v.27 no.4
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    • pp.482-488
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    • 2007
  • The optimum conditions for the extraction of lecithin from egg yolk were determined using response surface methodology (RSM). On the basis of the results of preliminary experiments, the most effective values were selected. The effects of three independent variables, dilution ratio, solvent composition, and extraction temperature on the response of crude egg lecithin (g) were then determined. The optimum conditions for egg lecithin separation obtained using ridge analysis were 6.51, 95.83%, and $40.2^{\circ}C$ for the dilution ratio, solvent composition, and extraction temperature, respectively. Using the optimum conditions, 55.04% of crude lecithin in total phospholipid can be obtained from 100 g liquid egg yolk. The experimental values (56.21% crude lecithin in total phospholipid) agreed with the predicted values.

Ten Cases of Taenia saginata Infection Confirmed by Analysis of the Internal Transcribed Spacer 1 rDNA Region in the Republic of Korea

  • Song, Su-Min;Yun, Hae Soo;VanBik, Dorene;Chang, Hyun-Ha;Lee, Sang-Ah;Kim, Shin-Woo;Ryoo, Namhee;Eun, Dong Yeub;Lee, Nan Young;Goo, Youn-Kyoung;Hong, Yeonchul;Ock, Meesun;Cha, Hee-Jae;Chung, Dong-Il
    • Parasites, Hosts and Diseases
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    • v.57 no.4
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    • pp.417-422
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    • 2019
  • From October 2015 to August 2018, tapeworm proglottids were obtained from 10 patients who were residents of Daegu and Gyeongbuk provinces and had a history of raw beef consumption. Most of them had no overseas travel experience. The gravid proglottids obtained from the 10 cases had 15-20 lateral uterine branches. A part of internal transcribed spacer 1 (ITS1) DNA of the 10 cases, amplified by polymerase chain reaction (PCR) and digested with AleI restriction enzyme, produced the same band pattern of Taenia saginata, which differentiated from T. asiatica and T. solium. Sequences of ITS1 and cytochrome c oxidase subunit 1 (cox1) showed higher homology to T. saginata than to T. asiatica and T. solium. Collectively, these 10 cases were identified as T. saginata human infections. As taeniasis is one of the important parasitic diseases in humans, it is necessary to maintain hygienic conditions during livestock farming to avoid public health concerns.

The Chemical Aspects on Hydrotreating Catalysis for Residue (잔사유의 수소화처리 촉매공정에 대한 화학적 고찰)

  • Jeon, Min-Seok;Lee, Youngjin;Jung, Hoi-Kyoeng;Kim, Hyung-Jong;Yoon, Seong-Ho;Kim, Taegon;Park, Joo-Il
    • Korean Chemical Engineering Research
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    • v.57 no.4
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    • pp.455-460
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    • 2019
  • Hydrotreating catalysis refers to a various hydrogenation which saturate an unsaturated hydrocarbon, together with removing heteroatoms such as sulfur, nitrogen, oxygen, and trace metals from different petroleum streams in a refinery. Most refineries include at least three hydrotreating units for upgrading naphtha, middle distillates, gas oils, intermediate process streams, and/or residue. Among them, hydrotreating catalysis for residue are the core of the process, because of its complexity. This article reviews recent progress in tackling the issues found in the upgrading residues by hydrotreating, focusing on the chemistry of hydrodemetallization (HDM) and hydrodesulfurization (HDS). We also discuss the composition and functions of hydrotreating catalysts, and we highlight areas for further improvement.