• Tuberculosis and Respiratory Diseases
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• 제51권3호
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• pp.201-210
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• 2001

연구배경 : 환경, 작업성노출, 바이러스감염, 유전적소인, 면역학적 이상 등 다양한 원인들이 특발성폐섬유화증(Idiopathic pulmonary fibrosis, IPF)의 원인인자들로 추정되어지고 있으나 아직 그 원인 및 병태생려는 분명하지 않다. 그런데, IPF환자의 10-13%는 폐암으로 사망하며, IPF에서 7배정도 폐암의 발생위험도 IPF 환자의 기관지 폐포상피세포가 p53과 p21단백을 과발현하고 있음이 보고되고 있고, 만성적인 유전자 손상의 결과로 이 두가지 단백의 발현이 증가되어 있을 것으로 추정된다. 방 법 : 연구자는 간질성 폐질환조직에서 p53과 K-ras단백의 발현정도와 임상양상을 관찰하고자 폐생검(개흉 폐생검 : 15예, 경기관지 폐생검 : 23예)조직에서 간질성 폐질환으로 진단된 38예를 대상으로 p53과 K-ras단백의 발현여부를 면역조직화학염색을 이용하여 관찰하였다. 결 과 : 간질성 폐질환 조직에서 p53은 21.1%에서, K-ras는 65.8%에서 암표지자단백 발현이 관찰되었다. 대조군으로 시행한 10예의 정상 기관지점막 표피세포는 전 예에서 두가지 p53, K-ras단백들이 발현되지 않았다. 간질성 폐질환의 조직형에 따라 암표지자 발현율이 차이를 보였는데, p53은 NSIP의 경우 36.4%로 양성율이 높았고, BOOP, AIP, DIP, UIP의 순이었다. K-ras는 전반적으로 p53에 비해 양성율이 높게 나타나서 UIP와 AIP가 75.0%로 가장 높았고, BOOP, DIP, NSIP의 순으로 나타났다. 이환기간과 암표지자 발현율과의 관계는 p53의 경우 증상이 오래 지속될수록 양성율이 높은 경향을 보였으며, K-ras의 경우는 증상의 기간과 관계없이 58-68%의 일정한 발현율을 보였고 전반적으로 p53보다 높은 양성율을 보였다. 결 론 : 본 연구의 결과 정상인의 상피세포에서는 관찰되지 않았으나 간질성 폐질환의 상피세포에서 p53과 K-ras단백의 발현이 증가되었음을 관찰할 수 있었고, 이러한 세포성장 또는 세포고사 조절인자들의 발현이 간질성 폐질환의 병태생리에 어떠한 역할을 하며 폐암의 발생과는 어떠한 관계에 있는지는 아직 분명하지 않으며 계속적인 연구가 요구된다.

• Elastomers and Composites
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• 제35권1호
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• pp.19-28
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• 2000

본 연구에서는 기존 외국제품들과의 물성 비교를 통해 부가중합형 실리콘 인상재를 개발하기 위한 기초연구를 수행하였다. IR과 EDX를 이용한 인상재들의 화학적 분석, DSC와 TGA를 이용한 열적거동분석, 접촉각, 점도, 가교특성 및 기계적특성 등의 분석을 수행하여 본 연구에서 개발된 제품의 고무인상재로의 응용 가능성을 평가하였다. 기초연구 결과에 따르면 인상재에 요구되는 모든 물성을 만족하는 제품은 없었지만, 본 연구에서 개발된 인상재의 경우 인장물성 및 가교밀도 특성이 상대적으로 우수하여 인상 채득시 요구되는 기계적 특성에는 가장 잘 부합하였다. 그러나 향후 친수성 계면활성제 도입을 통한 젖음성 향상, 온도와 경화시간과의 상관관계 확립, 정확한 체적변화 및 인열강도 개선 둥의 추가적인 물성 향상이 필요한 것으로 판단되었다.

• 대한환경공학회지
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• 제38권2호
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• pp.71-78
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• 2016

본 연구에서는 낙동강 창녕함안보 구간의 수질을 통계분석을 이용하여 수질 특성을 파악하였다. 다양한 통계분석기법 중에서 상관분석과 주성분분석 및 요인분석을 실시하였으며, 강우에 따른 조류의 천이양상을 연구하였다. 이는 향후 창녕함안보 구간의 수질관리정책 수립을 위한 기초 자료를 구축하는데 매우 중요한 자료이다. 측정 자료의 상관성 분석 결과, 클로로필-a와 조류는 상관성이 유의하지 않게 나타났으며, 요인분석 결과로 3개의 주성분이 추출되었으며, 제 1요인으로는 COD, TOC, BOD, pH, 수온이 분류되었다. 또한, 강우에 따른 조류의 천이양상을 분석한 결과, 강우 후에는 남조류의 수중개체 농도가 감소하는 추세를 보인 반면, 규조류와 녹조류의 개체 농도는 다소 증가하였다.

• 한국동물위생학회지
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• 제35권4호
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• pp.263-268
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• 2012

From the end of July 2012, several cases of abortion have been happened at the Korean indigenous cattle farm with 124 heads in Chungbuk province, Korea. Serological tests such as Rose-bengal test (RBT) and standard tube agglutination test (STAT) have been performed according to the standard official protocols of bovine brucellosis and 41 cattle turned out to be brucellosis-positive simultaneously. To find out the main factors of brucellosis outbreaks and spreads, additional serological, etiological and molecular investigation were applied. Totally, 11 B. abortus were isolated from 10 cattle's specimens including lymph-nodes and/or testis, and drinking water in cowhouse. In genotyping by multi-locus VNTR assay (MLVA) using 17 loci markers, the present B. abortus isolates were shown all the same pattern, D1 genotype, which has been reported in Gyeonggi and Gangwon province, Korea. These results suggest that the input of brucellosis might come from neighboring farms directly or indirectly, even if by unknown factor and expansion within farm would accelerate by materials related with aborting cows.

• Asian-Australasian Journal of Animal Sciences
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• 제27권11호
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• pp.1623-1629
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• 2014

Dry-cured pork neck samples were stored at $10^{\circ}C$ for 90 days under vacuum packaging (VP) or modified atmosphere packaging (MAP; 25% $CO_2$+75% $N_2$) conditions. The pH, moisture, water activity, total aerobic bacteria, and Enterobacteriaceae counts of dry-cured pork neck products with MAP were significantly lower than those with VP (p<0.05) after 90 days of storage. However, CIE $b^*$ and 2-thiobarbituric acid reacted substance (TBARS) values of the pork product with MAP were significantly higher (p<0.05) than those with VP. Total aerobic bacterial counts and Enterobacteriaceae counts of samples with MAP were lower than those with VP after 30 days of storage. Sensory results indicated that aroma, flavor and tenderness scores of the samples with both VP and MAP decreased during storage and the scores after >60 days of storage were lower than those at Day 1. In conclusion, despite presenting higher lipid oxidation, the samples stored in packages containing 25% $CO_2$ for 90 days at $10^{\circ}C$ have lower bacterial counts than vacuum-packed samples. Therefore, further studies should be performed on the packaging of dry-cured meat at adjusted concentrations of $CO_2$.

• Journal of Acupuncture Research
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• 제24권3호
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• pp.127-143
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• 2007

Objectives : The purpose of this study was to observe the effects of Chaenomelis Fructus herbal-acupuncture solution (ChF-HAS) at the Joksamni ($ST_{36}$) on arthritis induced by Collagen II in mice. Methods : The author performed several experimental items. The severity of arthritis, changes of mouse weight, size of the spleen and the degree of stenosis, changes of cytokine level, IgG, IgM and anti-collagen II, changes of immunocyte count, histological changes of the CIA mouse joint were analyzed and the conclusions are as follows. Results: 1. In the ChF-HA, the arthritis index, the incidence of arthritis, the degree of joint edema was significantly decreased. 2. In the ChF-HA, weight, spleen size and stenosis rate was low and maintained as the normal group was. 3. In the ChF-HA, cytokine level, IgG, IgM and anti-collagen II were significantly decreased. 4. In the ChF-HA, on changes of immunocyte count were maintained to the levels of normal group. 5. In histological changes of the CIA mouse joint, the cartilage destruction and synovial cell proliferation were decreased. Conclusions: These results suggest that ChF-HA at the $ST_{36}$ has an important role to control the immune reactions and suppress inflammatory response on the collagen induced rheumatoid arthritis. This study can be a significant supporting evidence that ChF-HA is chosen to be the principal therapy for clinical practice of the rheumatoid arthritis in the future.

• 대한수의학회지
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• 제54권4호
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• pp.197-201
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• 2014

In Korea, brucellosis has been reported periodically in cattle and rarely in dogs; however, it has not previously been screened in domestic animals such as elk, pigs and goats. To investigate the serological prevalence, serum samples were taken from the aforementioned animals annually during 2007-2013 and screened by the rose-bengal test (RBT) or modified RBT, after which positive sera were evaluated by the standard tube agglutination test (STAT). Finally, RBT and STAT-positive sera were confirmed by competitive-ELISA. Brucella abortus biovar 1 was isolated from three elk that were shown to be positive serologically in 2008. There was no evidence of brucellosis in pigs. Based on serological monitoring and investigation of etiological agents, there is no evidence of outbreak of brucellosis in elk, pigs or goats of Korea since 2008. However, the possibility for brucellosis from cattle to affect these other livestock exists; therefore, extensive and continuous serological monitoring is required to maintain their brucellosis-free status.

• 한국세라믹학회지
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• 제48권4호
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• pp.312-315
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• 2011

The effect of slurry composition and wafer flatness on a material removal rate (MRR) and resulting surface roughness which are evaluation parameters to determine the CMP characteristics of the on-axis 6H-SiC substrate were systematically investigated. 2-inch SiC wafers were fabricated from the ingot grown by a conventional physical vapor transport (PVT) method were used for this study. The SiC substrate after the CMP process using slurry added oxidizers into slurry consisted of KOH-based colloidal silica and nano-size diamond particle exhibited the significant MRR value and a fine surface without any surface damages. SiC wafers with high bow value after the CMP process exhibited large variation in surface roughness value compared to wafer with low bow value. The CMPprocessed SiC wafer having a low bow value of 1im was observed to result in the Root-mean-square height (RMS) value of 2.747 A and the mean height (Ra) value of 2.147 A.

• Journal of Microbiology and Biotechnology
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• 제24권12호
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• pp.1736-1743
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• 2014

In this study, we evaluated the effect of Lactobacillus plantarum HY7714 on skin hydration in human dermal fibroblasts and in hairless mice. In Hs68 cells, L. plantarum HY7714 not only increased the serine palmitoyltransferase (SPT) mRNA level, but also decreased the ceramidase mRNA level. In order to confirm the hydrating effects of L. plantarum HY7714 in vivo, we orally administered vehicle or L. plantarum HY7714 at a dose of $1{\times}10^9CFU/day$ to hairless mice for 8 weeks. In hairless mice, L. plantarum HY7714 decreased UVB-induced epidermal thickness. In addition, we found that L. plantarum HY7714 administration suppressed the increase in transepidermal water loss and decrease in skin hydration, which reflects barrier function fluctuations following UV irradiation. In particular, L. plantarum HY7714 administration increased the ceramide level compared with that in the UVB group. In the experiment on SPT and ceramidase mRNA expressions, L. plantarum HY7714 administration improved the reduction in SPT mRNA levels and suppressed the increase in ceramidase mRNA levels caused by UVB in the hairless mice skins. Collectively, these results suggest that L. plantarum HY7714 can be a potential candidate for preserving skin hydration levels against UV irradiation.

• Tuberculosis and Respiratory Diseases
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• 제69권5호
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• pp.375-380
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• 2010

Lymphoid interstitial pneumonia (LIP) is a rare benign lymphoproliferative interstitial lung disease. LIP has been associated with autoimmune disorders, HIV, viral infections, and so on. Once underlying systemic diseases have been excluded, a diagnosis of idiopathic LIP can be made. Although 6 cases of pathologically confirmed LIP have occurred in Korea, thus far none has been associated with primary Sjogren's syndrome. A 44-year-old man was admitted to hospital due to a dry cough and dypsnea on exertion that had been ongoing for 2 months. A chest radiography showed multiple and variable-sized cystic lesions, on both lungs and both interstitial infiltration and consolidation in both lower lung fields. Tests for autoantibody showed positive results of anti-nuclear antibody and anti-Ro/La antibody. The patient underwent a video assisted thoracoscopic surgery biopsy and pathologically confirmed LIP. We report the first known case of LIP-associated with primary Sjogren's syndrome in Korea.