• Tuberculosis and Respiratory Diseases
• /
• 제52권4호
• /
• pp.338-345
• /
• 2002

연구배경: 본 연구는 진폐증의 폐섬유화에 관여하는 사이토카인 중 PDGF-BB와 IGF-1의 혈청내 농도를 측정 비교함으로서 폐섬유화 관정에서의 역할을 간접적으로 확인하고 진폐증 진단의 생화학적 지표자로서 의미가 있는지 알아보고자, 충주의료원과 연세대학교 원주의과대학 원주기독병원에 내원한 환자를 대상으로 정상대조군과 단순형 석탄광부 진폐증군, 복잡형 석탄광부 진폐증군으로 나누어 혈청내 PDGF-BB와 IGF-1 농도를 측정하여 다음과 같은 결과를 얻었다. 방 법: 직업력과 방사선학적 소견으로 석탄광부 진폐증으로 진단된 환자중 단순형 석탄광부 진폐증 13예와 복잡형 석탄광부 진폐증 17예, 정상 대조군 10명을 대상으로 하였다. Human PDGF-BB immunoassay kit (R&D system, Minneapolis, MN)와 Human IGF-1 immunoassay kit (R&D system, Minneapolis, MN)를 이용하여 각 대상의 혈청내 PDGF-BB와 IGF-1의 농도를 측정하였다. 결 과: 1. 복잡형 석탄광부 진폐증군에서의 혈청 PDGF-BB 농도($10083.76{\pm}5639.07pg/mL$)가 정상 대조군 ($3726.17{\pm}1292.20pg/mL$)이나 단순형 석탄광부 진폐증($8493.88{\pm}5848.51pg/mL$)에 비해 통계학적으로 유의하게 높았다(P<0.05). 2. 정상대조군 ($413.40{\pm}61.94ng/mL$)과 단순형 석탄광부 진폐증($366.77{\pm}183.67ng/mL$), 복잡성 석탄광부 진폐증($403.40{\pm}115.39ng/mL$)의 혈청 IGF-1 농도는 각 군간 통계학적 유의한 차이는 관찰되지 않았다(P>0.05). 3. 작업년수에 따른 혈청 PDGF-BB와 IGF-1의농도는 통계학적으로 유의한 차이가 없었다(P>0.05). 결 론: 이상의 결과를 종합하여 보면 석탄광부 진폐증군에서 혈청내 PDGF-BB 농도의 증가는 폐섬유화 진행을 나타내는 생화학적 지표로서의 의미를 갖는 것으로 생각된다.

• 한국미생물·생명공학회지
• /
• 제50권4호
• /
• pp.533-547
• /
• 2022

This study investigated the hair growth effect of Schisandra chinensis extract (TS-SC) and TS-SC fermented by Lactobacillus plantarum (TS-SCLF) on human dermal papilla cells (hDPCs). The production of vascular endothelial growth factor (VEGF), insulin-like growth factor 1 (IGF-1), keratinocyte growth factor/fibroblast growth factor 7 (KGF/FGF-7) and hepatocyte growth factor (HGF), transforming growth factor beta 1 (TGF-β1) were examined. The secretion rates of VEGF and KGF/FGF-7 were high in TS-SC, and the secretion rates of IGF-1 and HGF were high in TS-SCLF. TGF-β1 was inhibited in a concentration-dependent manner in all samples. Gene expression of VEGF, IGF-1, KGF, HGF and alkaline phosphatase, relevant to hair growth, were examined. The data revealed that TS-SC and TS-SCLF successfully promoted hair growth in hDPCs. The IGF-1 gene was expressed in a dose-dependent manner in TS-SCLF. These results indicate that TS-SC and TS-SCLF fermented extract effectively promoted hair growth and gene expression relevant to hair growth in hDPCs. Used in clinical trials the test substance 'CMK-LPF01' showed a statistically significant increase in the number of hairs at 8 weeks, 16 weeks, and 24 weeks compared to before product use, and a change in hair growth, a secondary efficacy evaluation variable. Through additional research in the future, it is expected that "CMK-LPF01" can be developed as a functional material that can help alleviate symptoms of hair loss.

• The Korean Journal of Physiology and Pharmacology
• /
• 제20권5호
• /
• pp.459-466
• /
• 2016

Adipogenic differentiation of mesenchymal stem cells (MSCs) is critical for metabolic homeostasis and nutrient signaling during development. However, limited information is available on the pivotal modulators of adipogenic differentiation of MSCs. Adaptor protein Lnk (Src homology 2B3 [SH2B3]), which belongs to a family of SH2-containing proteins, modulates the bioactivities of different stem cells, including hematopoietic stem cells and endothelial progenitor cells. In this study, we investigated whether an interaction between insulin-like growth factor-1 receptor (IGF-1R) and Lnk regulated IGF-1-induced adipogenic differentiation of MSCs. We found that wild-type MSCs showed greater adipogenic differentiation potential than $Lnk^{-/-}$ MSCs. An ex vivo adipogenic differentiation assay showed that $Lnk^{-/-}$ MSCs had decreased adipogenic differentiation potential compared with wild-type MSCs. Interestingly, we found that Lnk formed a complex with IGF-1R and that IGF-1 induced the dissociation of this complex. In addition, we observed that IGF-1-induced increase in the phosphorylation of Akt and mammalian target of rapamycin was triggered by the dissociation of the IGF-1R-Lnk complex. Expression levels of a pivotal transcription factor peroxisome proliferator-activated receptor gamma ($PPAR-{\gamma}$) and its adipogenic target genes (LPL and FABP4) significantly decreased in $Lnk^{-/-}$ MSCs. These results suggested that Lnk adaptor protein regulated the adipogenesis of MSCs through the $IGF-1/Akt/PPAR-{\gamma}$ pathway.

• Molecules and Cells
• /
• 제25권3호
• /
• pp.358-367
• /
• 2008

The embryonic germ cell (EGCs) of mice is a kind of pluripotent stem cell that can be generated from pre- and post-migratory primordial germ cells (PGCs). Most previous studies on DNA methylation of EGCs were restricted to 12.5 days post coitum (dpc). This study was designed to establish and characterize murine EGC lines from migrated PGCs as late as 13.5 dpc and to estimate the degrees of methylation of their imprinted genes as well as of the non-imprinted locus, Oct4, using an accurate and quantitative method of measurement. We established five independent EGC lines from post migratory PGCs of 11.5-13.5 dpc from C57BL/6 ${\times}$ DBA/2 F1 hybrid mouse fetuses. All the EGCs exhibited the typical features of pluripotent cells including hypomethylation of the Oct4 regulatory region. We examined the methylation status of three imprinted genes; Igf2, Igf2r and H19 in the five EGC lines using bisulfite genomic sequencing analysis. Igf2r was almost unmethylated in all the EGC lines irrespective of the their sex and stage of isolation; Igf2 and H19 were more methylated than Igf2r, especially in male EGCs. Moreover, EGCs derived at 13.5 dpc exhibited higher levels of DNA methylation than those from earlier stages. These results suggest that in vitro derived EGCs acquire different epigenotypes from their parental in vivo migratory PGCs, and that sex-specific de novo methylation occurs in the Igf2 and H19 genes of EGCs.

• Asian-Australasian Journal of Animal Sciences
• /
• 제24권10호
• /
• pp.1393-1398
• /
• 2011

Four 4-month old Charolais${\times}$Dorset male sheep (initial liveweight $25.0{\pm}1.1\;kg$), fitted with rumen and abomasal fistulas and nourished by total intragastric infusions, were used to study the relationships between methionine (Met) supply, nitrogen (N) retention and plasma insulin-like growth factor-I (IGF-I). Four graded levels of Met, i.e. 0 g/16 g N, 1.76 g/16 g N, 3.52 g/16 g N and 7.04 g/16 g N, were infused into abomasums as experimental treatments. The sheep and treatments were allocated in a $4{\times}3$ incomplete Latin square design (Yudon square design). The experiment lasted 3 periods and each period was 10 days. Quadratic correlations were found between Met level (x, g/16 g N) and N retention (y, g/d): y = $-0.03x^2$+0.41x+2.62, $r^2$ = 0.66, n = 12, p = 0.008, and between methionine level (x, g/16 g N) and plasma IGF-I concentration (y, ng/ml): y = $0.80x^2$-4.53x+190.24, $r^2$ = 0.51, n = 12, p = 0.009. No significant correlation was found between plasma IGF-I (x, ng/ml) and N retention (y, g/d) (p>0.05). It was concluded that Met level had a significant influence on N retention and plasma IGF-I concentration whereas IGF-I might not be an important mediator in the regulation of N metabolism by Met in growing sheep nourished by total intragastric infusions.

• Asian-Australasian Journal of Animal Sciences
• /
• 제23권6호
• /
• pp.693-699
• /
• 2010

Imprinted genes are essential for fetal development, growth regulation, and postnatal behavior. However, little is known about imprinted genes in livestock. We hypothesized that certain putatively imprinted genes affected normal peri-implantation development such as embryo elongation, initial placental development, and preparation of implantation. The objective of the present study was to investigate the mRNA expression patterns of several putatively imprinted genes during the porcine peri-implantation stages from day 6 to day 21 of gestation. Imprinted genes were selected both maternally (Dlk1, IGF2, Ndn, and Sgce) and paternally (IGF2r, H19, Gnas and Xist). Here, we report that the maternally imprinted gene IGF2 was expressed from day 6 (Blastocyst stage), but Dlk1, Ndn, and Sgce were not expressed in this stage. These genes were first expressed between days 12 and day 14. All the maternally imprinted genes studied showed significantly high expression patterns from day 18 of embryo development. In contrast, paternally imprinted genes IGF2r, H19, Gnas, and Xist were first expressed from day 6 of embryo development (BL). Our data demonstrated that the expression of H19 and Gnas genes was significantly increased from day 14 of the embryo developmental stage, while IGF2r and Xist only showed high expression after day 21. This study is the first to show that the putatively imprinted genes were stage-specific during porcine embryonic development. These results demonstrate that the genes studied may exert important effects on embryo implantation and fetal development.

• 한국동물번식학회:학술대회논문집
• /
• 한국동물번식학회 2004년도 춘계학술발표대회
• /
• pp.290-290
• /
• 2004

유전자재조합 소 소마토트로핀(recombinant bovine somatotropin, rBST)은 인슐린양성장인자(IGF)-1의 합성과 분비량 조절에 의해 당과 아미노산의 흡수 및 단백질합성의 촉진을 통한 체내흡수 영양소 이용효율 증가, 산유량 증진 및 증체 효과, 세포증식의 촉진작용 등이 있는 것으로 보고(Bauman, 1982; Mears, 1995; 권 등, 1997)된 바 있으며, 또한 최근에 젖소 및 육우빈우의 인공수정시 rBST를 투여하였을 때 1회 수정수태율, 혈중 progesterone 농도 및 IGF-1 농도 등에 영향을 미치는 것으로 보고하였다(Lucy 등, 1994; Bilby 등, 1999; Morales-Roura 등, 2001). (중략)

• 한국수정란이식학회지
• /
• 제25권2호
• /
• pp.127-131
• /
• 2010

The objective of this study was to determine the mRNA expression patterns of several putative imprinted genes in in vivo and in vitro fertilized, parthenogenetic, and cloned porcine preimplantation embryos. Both maternally (Dlk1, IGF2, Peg1/Mest and Ndn) and paternally (IGF2r, H19 and Xist) imprinted genes were selected. We have used reverse transcription polymerase chain reaction (RT-PCR) to investigate gene expression patterns in the porcine embryos. IGF2 transcripts were detected in the most of embryos. In nuclear transfer (NT), Peg1/MEST transcripts showed fluctuating pattern. Dlk1 was only expressed partially from the morula and blastocyst stage of NT embryos. Ndn gene expression was started somewhat early for in vivo embryos. However, the expressions of maternally imprinted genes were similar in all types of blastocysts (NT, in vivo and in vitro fertilized, and parthenogenetic embryos). The IGF2R gene expression level was somewhat irregular and varied among samples. However, for the majority samples of all types of embryos, IGF2R expression was diminished after one- to two-cell stages and reappeared at the morulae or blastocyst stage embryos. H19 gene was only expressed early in parthenogenetic and in vivo embryos. For NT embryos, H19 was only expressed in blastocysts. Xist expression was detected in all blastocysts with the earliest being in vivo 8-cell stage embryos and the last one being NT blastocysts. These putative imprinted genes appeared to have stage specific expression patterns with a fluctuating pattern for some genes (Peg/Mest, IGF2r, H19). These results suggest that stage specific presence of imprinted genes can affect the embryo implantation and fetal development.

• Clinical and Experimental Pediatrics
• /
• 제51권7호
• /
• pp.722-728
• /
• 2008

목 적 : 건강한 만삭아의 제대혈에서 아디포넥틴, 렙틴, 인슐린, IGF-I, IGFBP-3를 측정하여 출생체중, 신장, 지방량과의 관계를 알아보고자 하였다. 또한 적정 체중아 그룹에서 이 호르몬들의 출생후 1개월 동안 변화에 대해서 살펴보기로 하였다. 방 법 : 임신과 관련된 합병증이 없었던 산모에서 태어난 200명의 건강한 만삭아(남아 109명, 여아 91명)를 대상으로 하여 제대혈을 채취하여 혈장과 혈청을 분리하였고 출생체중, 출생신장, 머리둘레, 가슴둘레, 피부두께를 측정하였으며 폰더랄지수를 계산하였다. 대상 신생아들을 출생체중에 따라 AGA (n=132), SGA (n=29), LGA (n=39)의 세 그룹으로 나누었다. 적정체중아 중 15명에서 생후 3일, 7일, 30일에 신생아의 혈액을 채취하여 제대혈과 같은 방법으로 분리하였고 체중과 신장을 측정하였다. 결 과 : 아디포넥틴과 인슐린, IGF-I은 AGA, LGA군보다 SGA군에서 더 낮았다. 렙틴은 AGA, SGA군보다 LGA군에서 더 높았다. 아디포넥틴과 렙틴, 인슐린, IGF-I, IGFBP-3는 출생체중과 피부두께의 합과 양의 상관관계에 있었다. 출생 시 신장과 양의 상관관계를 보인 호르몬은 아디포넥틴, 렙틴, IGF-I이었다. 아디포넥틴은 렙틴과 양의 상관관계를 보였으나 인슐린, IGF-I, IGFBP-3와는 상관관계가 없었다. IGF-I은 남아보다 여아에서 더 높은 수치를 보였다. 출생 후 1개월 동안 렙틴은 생후 7일까지는 생리적 체중감소와 더불어 감소하다가 그 후 증가하였고 IGF-I 또한 생후 3일에 감소하다가 1개월 후 급격한 증가를 보였다. 결 론 : 아디포넥틴과 렙틴, 인슐린, IGF-I, IGFBP-3 모두 태아의 성장에 중요한 역할을 하며, 아디포넥틴은 인슐린, IGF-I 축과는 다른 기전으로 태아의 성장을 조절한다고 생각할 수 있었다. IGF-I은 남아에서보다 여아에서 더 높은 수치를 보여서 IGF-I의 성별간의 차이가 자궁 내에서도 존재하였다. 생후 1개월 동안의 성장에 다른 호르몬보다 IGF-I 이 더 중요한 역할을 한다는 사실을 알 수 있었다.

• 한국가축번식학회지
• /
• 제23권4호
• /
• pp.323-331
• /
• 1999

본 연구는 돼지난자의 체외성숙동안 insulin-like growth factor-I (IGF-I)과 난구세포의 영향을 검토하기 위하여 실시하였다. 미성숙난자를 0(60%), 1(61%), 5(62%) 및 l0ng/$m\ell$(72%)의 서로 다른 IGF-I의 농도로 첨가하여 배양했을 때 체외성숙율은 큰 차이를 나타내지 않았다. 또한 10ng/$m\ell$의 IGF-I이 첨가된 배양액내에서 난자를 배양한 경우, 난구세포부착시 (68%) 제거 (52%)된 난자에 비해 높은성숙율을 나타냈지만 유의적인 차이는 인정되지 않았다. 그러나 IGF-I이 첨가되지 않은 경우에는 난구세포 부착 (63%) 난자가 제거 (32%)된 난자에 비해 유의적 (P＜0.05)으로 높은 성숙율을 나타냈으며, IGF-I를 성숙 배양기간중 전반기 24시간 또는 후반기 24시간 동안만 첨가했을 때의 난구세포 부착시 (61과 49%) 제거된 (45 와 49%) 난자에 비해 높은 성숙융올 나타냈다. 한편, IGF-I의 존재 여부에 관계없이 FCS와 돼지난포액 (PFF)이 무첨가된 배양액에서 48시간동안 또는 배양전반기 24시간동안 난구세포를 제거한 경우 (16과 18%)로 난구세포 부착시 (46과 63%)에 비해 유의적 (P＜0.01)으로 낮은 성숙율을 나타냈다. 이와 같은 결과는 난구세포가 IGF-I의 존재 유무에 관계없이 난자의 체외성숙에 필수적이며, FCS와 PFF 첨가시 난구세포가 제거된 난자의 체외성숙을 촉진하는 것으로 밝혀졌다.