Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Hair Growth Effect of TS-SCLF from Schisandra chinensis Extract Fermented with Lactobacillus plantarum

  • Young Min, Woo (Department of Pharmaceutical Engineering, College of Medical and Life Science, Silla University) ;
  • Jae Yong, Seo (Creative Innovation Research Center, Cosmecca Korea, Co., Ltd.) ;
  • Soo-ya, Kim (Department of Pharmaceutical Engineering, College of Medical and Life Science, Silla University) ;
  • Ji Hyun, Cha (Department of Pharmaceutical Engineering, College of Medical and Life Science, Silla University) ;
  • Hyun Dae, Cho (Creative Innovation Research Center, Cosmecca Korea, Co., Ltd.) ;
  • Young Kwon, Cha (Creative Innovation Research Center, Cosmecca Korea, Co., Ltd.) ;
  • Ju Tae, Jeong (Creative Innovation Research Center, Cosmecca Korea, Co., Ltd.) ;
  • Sung Min, Park (Bio Convergence R&D Center, CoseedBioPharm, Co., Ltd.) ;
  • Hwa Sun, Ryu (Bio Convergence R&D Center, CoseedBioPharm, Co., Ltd.) ;
  • Jae Mun, Kim (Bio Convergence R&D Center, CoseedBioPharm, Co., Ltd.) ;
  • Moon Hoy, Kim (Bio Convergence R&D Center, CoseedBioPharm, Co., Ltd.) ;
  • Hee-Taek, Kim (Department of Korean Medical Ophthalmology & Otolaryngology & Dermatology, College of Korean Medicine, Semyung University) ;
  • Yong-Min, Kim (Department of Cosmetic Sciences and Beauty Biotechnology, Semyung University) ;
  • Kwang Sik, Joo (R&D Center, TS Trillion, Co., Ltd.) ;
  • Sun Mi, Lee (R&D Center, TS Trillion, Co., Ltd.) ;
  • JungNo, Lee (Bio Convergence R&D Center, CoseedBioPharm, Co., Ltd.) ;
  • Andre, Kim (Department of Pharmaceutical Engineering, College of Medical and Life Science, Silla University)
  • Received : 2022.06.29
  • Accepted : 2022.11.29
  • Published : 2022.12.28
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Abstract

This study investigated the hair growth effect of Schisandra chinensis extract (TS-SC) and TS-SC fermented by Lactobacillus plantarum (TS-SCLF) on human dermal papilla cells (hDPCs). The production of vascular endothelial growth factor (VEGF), insulin-like growth factor 1 (IGF-1), keratinocyte growth factor/fibroblast growth factor 7 (KGF/FGF-7) and hepatocyte growth factor (HGF), transforming growth factor beta 1 (TGF-β1) were examined. The secretion rates of VEGF and KGF/FGF-7 were high in TS-SC, and the secretion rates of IGF-1 and HGF were high in TS-SCLF. TGF-β1 was inhibited in a concentration-dependent manner in all samples. Gene expression of VEGF, IGF-1, KGF, HGF and alkaline phosphatase, relevant to hair growth, were examined. The data revealed that TS-SC and TS-SCLF successfully promoted hair growth in hDPCs. The IGF-1 gene was expressed in a dose-dependent manner in TS-SCLF. These results indicate that TS-SC and TS-SCLF fermented extract effectively promoted hair growth and gene expression relevant to hair growth in hDPCs. Used in clinical trials the test substance 'CMK-LPF01' showed a statistically significant increase in the number of hairs at 8 weeks, 16 weeks, and 24 weeks compared to before product use, and a change in hair growth, a secondary efficacy evaluation variable. Through additional research in the future, it is expected that "CMK-LPF01" can be developed as a functional material that can help alleviate symptoms of hair loss.

Keywords

Acknowledgement

This study was supported by the Technology Innovation Program (10077377, Development of hair and anti-aging cosmetic materials using lactic acid bacteria enzyme hydrolysates) funded By the Ministry of Trade, Industry & Energy (MOTIE, Korea) and supported by the Brain Busan 21+ project (BB21+).

References

  1. Lee KM, Lee JS, Oh JY, Kim YC. 2010. The hair growth effect of Polygoni mulitiflori radix water extracts in C57BL/6 mice. J. Cosmetol. Sci. 6: 419-426. 
  2. Do EJ, Hwang MY, Kim SY, Lee JS, Yang DS, Yang CH, et al. 2011. The effect of Gyungokgo-gamibang extract on hair growth and protein expression in mice. Korean J. Herbol. 26: 9-14. 
  3. Ryu EM, Shin HJ. 2011. Hair growth effect of ethyl acetate and water fractions of Sparassis crispa extracts on hair-removed C57BL/6 mice. Asian J. Beauty Cosmetol. 9: 1-10. 
  4. Park HY, Kim SN, Kang BH, Lee JH. 2010. Experimental studies of Glycine max Merr. (black bean), Triticum aestivum L. (wheat) and Oryza sativa L. (rice bran) extracts on the effects of hair growth activity and physical properties. Korean J. Oriental Med. 16: 167-173. 
  5. Ryu EM, Shin HJ, Na MS. 2010. Effect of Sparassis crispa extracts on hair growth inhibition of hair-removed C57BL/6 mice. Asian J. Beauty Cosmetol. 8: 1-11. 
  6. Park KS, Park DH. 2016. Comparison of Saccharina japonica-Undaria pinnatifida mixture and minoxidil on hair growth promoting effect in mice. Arch. Plast. Surg. 43: 498-505.  https://doi.org/10.5999/aps.2016.43.6.498
  7. Kim JY, Shin JY, Choi YH, Nam YJ, Lee SY, Jeon JH, et al. 2019. Hair growth promoting effect of Hottuynia cordata extract in cultured human hair follicle dermal papilla cells. Biol. Pharm. Bull. 42: 1665-1673.  https://doi.org/10.1248/bpb.b19-00254
  8. Ryu EM, Seo GW, Kee KH, Shin HJ. 2013. Effect of ethanolic extract from wheat sprout on hair growth of C57BL/6 mouse. Korean J. Aesthet. Cosmetol. 11: 1051-1057. 
  9. Kim JH, Lee SO, Beik GY, Kim KJ. 2021. Effects of Aucklandia lappa Decne. extract on hair growth in depilated CL57BL/6 mice. Korean J. Clin. Lab. Sci. 53: 88-95.  https://doi.org/10.15324/kjcls.2021.53.1.88
  10. Chen Y, Huang J, Chen R, Yang L, Wang J, Liu B, et al. 2020. Sustained release of dermal papilla-derived extracellular vesicles from injectable microgel promotes hair growth. Theranostics 10: 1454-1478.  https://doi.org/10.7150/thno.39566
  11. Han JH, Kwon OS, Chung JH, Cho KH, Eun HC, Kim KH. 2004. Effect of minoxidil on proliferation and apoptosis in dermal papilla cells of human hair follicle. J. Dermatol. Sci. 34: 91-98.  https://doi.org/10.1016/j.jdermsci.2004.01.002
  12. Oh HJ, Park HK, Ly SY. 2012. An assessment of hair loss ameliorating effect of medicinal plant extracts, amino acid and vitamin complex in human subject. Korean J. Hum. Ecol. 21: 587-596.  https://doi.org/10.5934/KJHE.2012.21.3.587
  13. McClellan KJ, Markham A. 1999. Finasteride. Adis Drug Evaluation 57: 111-126. 
  14. Hsu CL, Liu JS, Lin AC, Yang CH, Chung WH, Wu WG. 2014. Minoxidil may suppress androgen receptor-related functions. Oncotarget 5: 2187-2197.  https://doi.org/10.18632/oncotarget.1886
  15. Shapiro J, Price VH. 1998. Hair regrowth: Therapeutic agents. Dermatol. Clin. 16: 341-356.  https://doi.org/10.1016/s0733-8635(05)70017-6
  16. An GS, Hwang IC. 2009. Hair loss prevention and hair growth promotion by herb extracts which contain the cultured Korean wild ginseng. J. Korea Soc. Beauty Art 10: 221-226. 
  17. Choi HM, Hwang SJ, Lee JS, Do EJ, Kim MY, Kim MR. 2011. Effects of hair tonic and food including Korean medicinal herbs on hair growth in an alopecia model of C57BL/6 mice. Korean J. Herbol. 26: 119-124. 
  18. Jeon HY, Kim SH, Kim CW, Shin HJ, Seo DB, Lee SJ. 2011. Hair growth promoting effect of black soybean extract in vitro and in vivo. Korean J. Food Sci. Technol. 43: 747-753.  https://doi.org/10.9721/KJFST.2011.43.6.747
  19. Woo YM, Kim OJ, Jo ES, Jo MY, Ahn MY, Lee YH, et al. 2019. The effect of Lactobacillus plantarum hydrolysates promoting VEGF production on vascular growth and hair growth of C57BL/6 mice. J. Anal. Sci. Technol. 10: 18-27.  https://doi.org/10.1186/s40543-019-0178-0
  20. Joo SS. 2011. In vitro and in vivo hair growth promotion effects of Lactobacillus plantarum-fermented plant extracts (MBN). Korean J. Food Sci. Technol. 43: 381-386.  https://doi.org/10.9721/KJFST.2011.43.3.381
  21. Kim JY, Shin JY, Choi UH, Jang M, Nam YJ, Lee SY, et al. 2019. Hair growth promoting effect of Hoyyuynia cordata extract in cultured human hair follicle dermal papilla cells. Biol. Pharm. Bull. 42: 1665-1673.  https://doi.org/10.1248/bpb.b19-00254
  22. Nagappan A, Jung DY, Kim JH, Lee HY, Jung MH. 2018. Gomisin N alleviates ethanol-induced liver injury through ameliorating lipid metabolism and oxidative stress. Int. J. Mol. Sci. 19: 2601. 
  23. Lee JN, Ryu HS, Kim JM, Jung TH, Park SM, Lee YM. 2017. Antimelanogenic effect of gomisin N from Schisandra chinensis (Turcz.) Baillon (Schisandraceae) in melanoma cells. Arch. Pharm. Res. 40: 807-817.  https://doi.org/10.1007/s12272-017-0903-4
  24. Oh SY, Kim YH, Bae DS, Um BH, Pan CH, Kim CY, et al. 2010. Anti-inflammatory effects of gomisin N, gomisin J, and schisandrin C isolated from the fruit of Schisandra chinensis. Biosci. Biotechnol. Biochem. 74: 90597-1-7.  https://doi.org/10.1271/bbb.90682
  25. Jang MK, Yun YR, Kim SH, Kim JH, Jung MH. 2016. Protective effect of gomisin N against endoplasmic reticulum stress-induced hepatic steatosis. Biol. Pharm. Bull. 39: 832-838.  https://doi.org/10.1248/bpb.b15-01020
  26. Heo JH, Park JG, Cheon HJ, Kim YS, Kang SS, Hung TM, et al. 2006. Hepatoprotective activities of gomisin A and gomisin N. Korean J. Pharmacogn. 37: 294-301. 
  27. Cho HD, Jeong JY, Ryu HS, Lee JN, Park SM. 2020. Dermal papilla cells proliferation constituent of Schisandra chinensis fruits and optimization using response surface methodology. J. Soc. Cosmet. Sci. Korea 46: 415-424.  https://doi.org/10.15230/SCSK.2020.46.4.415
  28. Woo YM, Kim OK, Jo ES, Jo MY, Ahn, MY, Lee YH, et al. 2019. The effect of Lactobacillus plantarum hydrolysates promoting VEGF production on vascular growth and hair growth of C57BL/6 mice. J. Anal. Sci. Technol. 10: 1-9.  https://doi.org/10.1186/s40543-018-0160-2
  29. Gnabre J, Unlu I, Chang TC, Lisseck P, Bourne B, Scolnik R, et al. 2010. Isolation of lignans from Schisandra chinensis with anti-proliferative activity in human colorectal carcinoma: Structure-activity relationships. J. Chromatogr. B 878: 2693-2700.  https://doi.org/10.1016/j.jchromb.2010.08.005
  30. Madaan A, Joshi V, Kishore A, Verma R, Singh AT, Jaggi M, et al. 2017. In vitro hair growth promoting effects of naringenin and hesperetin on human dermal papilla cells and keratinocytes. Am. J. Dermatol. Venereol. 6: 51-57. 
  31. Wakame K, Nakata A, Sato K, Mihara Y, Takanari J, Sato A, et al. 2017. Comparison of effects on gene expression activity of low-molecular-weight lychee fruit polyphenol (Oligonol®), adenosine, and minoxidil in human dermal papilla cells. J. Biomed. Transl. Res. 3: 5-12.  https://doi.org/10.14710/jbtr.v3i1.928
  32. Tsuboi R, Sato C, Kurita Y, Ron D, Rubin JS, Ogawa H. 1993. Keratinocyte growth factor (FGF-7) stimulates migration and plasminogen activator activity of normal human keratinocytes. J. Invest. Dermatol. 101: 49-53.  https://doi.org/10.1111/1523-1747.ep12358892
  33. Hue JJ, Jo BK, Kang BS, Kim JH, Nam SY, Yun YW, et al. 2010. Effect of sodium silicate on hair growth in C57BL/6 mice. Lab. Anim. Res. 26: 55-62.  https://doi.org/10.5625/lar.2010.26.1.55
  34. Kim YE, Choi HC, Nam G, Choi BY. 2019. Costunolide promotes the proliferation of human hair follicle dermal papilla cells and induces hair growth in C57BL/6 mice. J. Cosmet. Dermatol. 18: 414-421.  https://doi.org/10.1111/jocd.12674
  35. Paus R, Foitzik K, Welker P, Bulfone-Paus S, Eichmuller S. 1997. Transforming growth factor-β receptor type I and type II expression during murine hair follicle development and cycling. J. Invest. Dermatol. 109: 518-526.  https://doi.org/10.1111/1523-1747.ep12336635
  36. Yamauchi K, Kurosaka A. 2009. Inhibition of glycogen synthase kinase-3 enhances the expression of alkaline phosphatase and insulin-like growth factor-1 in human primary dermal papilla cell culture and maintains mouse hair bulbs in organ culture. Arch. Dermatol. Res. 301: 357-365. https://doi.org/10.1007/s00403-009-0929-7