• 제목/요약/키워드: IE Technique

검색결과 137건 처리시간 0.037초

Yeast 2 $\mu$m 플라스미드 유래 FLP recombinase 유전자의 곤충 배양세포내 발현 (Expression of the FLP recombinase of the 2 $\mu$m plasmid of yeast in the cultured cells of Bombyx mori using a transient expression vector)

  • 강석우;윤은영
    • 한국잠사곤충학회지
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    • 제39권1호
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    • pp.36-43
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    • 1997
  • In order to express the FLP recombinase in B. mori cultured cell line, BmN-4, transient expression system using a heat shock protein gene (hsp70) promoter of Dorosophilla melnogaster was constructed. This vector was designated as pHsSV. Activity strength of the hsp70 promoter was compared with that of immediate early gene (IE-1) and polyhedrin gene of BmNPV employing the E. coli $\beta$-galactosidase gene as a reporter gene. The result showed that the pHs $\beta$-gal plasmid vector expressed the $\beta$-galactosidase at 2nd and 3rd day after the transfer of plasmid DNA into BmN-4 cells, which was similar to that of pIE1 $\beta$-gal vector, but different from that of a recombinant virus, vBm $\beta$-gal. For the construction of FLP recombinase transient expression vector, the FLP recombinase gene was cloned by polymerase chain reaction technique. To express the FLP recombinase, this gene was inserted into pHsSV plasmid vector, under the control of the hsp70 promotor, and tranfected in BmN-4 cells. The expressed FLP recombinase was estimated at 44kDa on a 12.5% SDS-PAGE.

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파이썬 모듈과 정규표현식을 활용한 웹 취약점 탐색 자동화 봇 (Browser fuzzing and analysis using known vulnerability)

  • 김남규;김기환;이훈재
    • 한국정보통신학회:학술대회논문집
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    • 한국정보통신학회 2016년도 춘계학술대회
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    • pp.749-751
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    • 2016
  • 웹이 보편화되면서 많은 편리함을 가져온 동시에 주 사용자인 기업에는 대외비 문서서, 중요 자원의 유출, 개별 사용자에게는 개인 정보 유출 등의 보안 이슈 또한 가져왔다. 이에 관련 기관들은 웹에 대한 취약점을 정리한 리스트를 제시하였다. 본 지에서는 웹 취약점의 표준으로 널리 사용되는 OWASP top10의 리스트들을 웹에서 자동으로 탐색하고 문서화하는 봇의 구현 모델을 제시한다.

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고온 초전도체의 임계전류 저하에 따른 교류 손실 특성 (Ac Loss Characteristics under Critical Current Degradation of HTS Tapes)

  • 김해준;김재호;심기덕;조전욱;곽동순;김해종;성기철
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2005년도 하계학술대회 논문집 Vol.6
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    • pp.286-287
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    • 2005
  • Critical current(Ic) degradation of HTS tapes and AC loss under mechanical load is one of the hottest issues in HTS development and application. Mechanical load reduces the critical current of superconducting wire, and the Ie degradation affects the AC loss of the wire. We measured the Ie degradation and AC loss under tension and bending of Bi-2223 tapes processed by "Powder-in-Tube" technique at 17K with self-field. And we have studied the frequency dependence of self-field AC loss in multi-filamentary Bi-2223/Ag tape at 77K. The measurement results and discussions on the relationship between Ic degradation and AC loss are presented.

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IMM Method Using Intelligent Input Estimation for Maneuvering Target Tracking

  • Lee, Bum-Jik;Joo, Young-Hoon;Park, Jin-Bae
    • 제어로봇시스템학회:학술대회논문집
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    • 제어로봇시스템학회 2003년도 ICCAS
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    • pp.1278-1282
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    • 2003
  • A new interacting multiple model (IMM) method using intelligent input estimation (IIE) is proposed to track a maneuvering target. In the proposed method, the acceleration level for each sub-model is determined by IIE-the estimation of the unknown acceleration input by a fuzzy system using the relation between maneuvering filter residual and non-maneuvering one. The genetic algorithm (GA) is utilized to optimize a fuzzy system for a sub-model within a fixed range of acceleration input. Then, multiple models are composed of these fuzzy systems, which are optimized for different ranges of acceleration input. In computer simulation for an incoming ballistic missile, the tracking performance of the proposed method is compared with those of the input estimation (IE) technique and the adaptive interacting multiple model (AIMM) method.

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GA-Based Fuzzy Kalman Filter for Tracking the Maneuvering Target

  • Noh, Sun-Young;Lee, Bum-Jik;Joo, Young-Hoon;Park, Jin-Bae
    • 제어로봇시스템학회:학술대회논문집
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    • 제어로봇시스템학회 2005년도 ICCAS
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    • pp.1500-1504
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    • 2005
  • This paper proposes the design methodology of genetic algorithm (GA)-based fuzzy Kalman filter for tracking the maneuvering target. The performance of the standard Kalman Filter (SKF) has been degraded because mismatches between the modeled target dynamics and the actual target dynamics. To solve this problem, we use the method to estimate the increment of acceleration by a fuzzy system using the relation between maneuver filter residual and non-maneuvering one. To optimize the fuzzy system, a genetic algorithm (GA) is utilized and this is then tuned by the fuzzy logic correction. Finally, the tracking performance of the proposed method has been compared with those of the input estimation (IE) technique and the intelligent input estimation (IIE) through computer simulations.

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작업관리기법의 활용실태에 관한 조사연구 (A study on the work study application in industry)

  • 안세희
    • 기술사
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    • 제16권4호
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    • pp.20-31
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    • 1983
  • Study was made for the work study application in industry by sampling one thousand manufacturing companies. Small and medium industries are far behind in systematic application of the work study technique. Large industries are gradually introducing the work study technique systematically in line with the nation-wide quality control movement. Major sessions that the industries feel important in IE curriculum were QC, Work study and Cost accounting followed by the Organization theory, Industrial psychology, and Labor relations. Most of the industries were still less aware of the sophisticated fields such as OR, SE and Simulation. They put more emphasis on QC and TQC and as a result, work study should be closely bounded to TQC in order to systemize the work study application in the future.

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재고통제기법을 이용한 효율적 포트폴리오 관리 방안 (A Methodology for Efficient Portfolio Management Using Inventory Control Technique)

  • 유재필;신현준
    • 산업공학
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    • 제25권2호
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    • pp.163-169
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    • 2012
  • This paper proposes an efficient portfolio management methodology named sSPPM with consideration of risk and required return. sSPPM employs Markowitz's portfolio model to select securities and adopts ($s$, $S$) policy that is a well-known technique in the inventory control area to revise the current portfolio. Computational experiments using virtual stock prices generated by monte carlo simulation method as well as real stock ones of KOSPI for recent 4 years are conducted to show the excellence of the portfolio management under ($s$, $S$) policy framework. The result shows that sSPPM is remarkably superior to both 6 or 12 months based periodic portfolio revision method and market (KOSPI index).

기계학습 기반의 웹 마이닝을 이용한 고객 세분화에 관한 연구 (Identification of Customer Segmentation Sttrategies by Using Machine Learning-Oriented Web-mining Technique)

  • 이건창;정남호
    • 산업공학
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    • 제16권1호
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    • pp.54-62
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    • 2003
  • With the ubiquitous use of the Internet in daily business activities, most of modern firms are keenly interested in customer's behaviors on the Internet. That is because a wide variety of information about customer's intention about the target web site can be revealed from IP address, reference address, cookie files, duration time, all of which are expressing customer's behaviors on the Internet. In this sense, this paper aims to accomplish an objective of analyzing a set of exemplar web log files extracted from a specific P2P site, anti identifying information about customer segmentation strategies. Major web mining technique we adopted includes a machine learning like C5.0.

OFDM 기반 광대역 멀티미디어 단말의 전력절감 효율 분석에 관한 연구 (Investigation of Power Saving Efficiency for the OFDM Based Multimedia Communication Terminal)

  • 문재필;이은서;김동환;이재식;장태규
    • 대한전자공학회:학술대회논문집
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    • 대한전자공학회 2005년도 추계종합학술대회
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    • pp.155-158
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    • 2005
  • An invesitigation on power consumption of a mobile multimedia system using OFDM and MDVS technique is reported here. Analysis and simulation are performed to find the significances of proposed Microscopic Dynamic Voltage Scaling(MDVS) tehnique[4] on digital processor in terms of power saving. A study is also made to show power reduction in mobile multimedia system by incorporating OFDM modulation scheme in RF front-end. Finally, overall power consumption by functionally distinguished blocks ie. RF front-end, digital processor and human interface unit is shown here. Total power consumption is 8.2W for 2Mbps SD-quality WCDMA multimedia video service - the power consumption of digital processor is 3.9W(48%), the power consumption of RF front-end is 3.2W (36%), and the power consumption of interface is 1.8W(16%). Power saving of applying purposed MDVS technique is 35% in digital processor, and power saving of OFDM technique is 10-12dB in RF front-end.

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열차제어기술 체계화 방안에 대한 연구 (A Study of Systematization for Train Control Technique)

  • 이재호;신덕호;이강미
    • 한국철도학회:학술대회논문집
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    • 한국철도학회 2009년도 춘계학술대회 논문집
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    • pp.2010-2016
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    • 2009
  • Before introducing high speed train, train signal system was operated passively depending on a driver by signal lamp display. Now it is changing to onboard signaling system because of train speed increased(conventional track is 230km/h, high speed track is 380km/h), high speed. low speed freight train operation mixed, operation for high speed train in conventional track and dense operation. ie. ETCS(European Train Control System) Level 1 is introducing. Also, in case of high speed train, the train control system of France was introduced and has operated from 2004, now we have a difficulty for rising speed more than 300km/h because of commercial operation speed limited as 300km/h. Therefore, it need to establish the train control technique according to trackside surroundings and develope standard system like European ERTMS/ETCS, China CTCS(Chinese Train Control System), Japan D-ATC(Digital Automatic Train Control). In this paper, we derive the systematization method for Korea train technique by network-oriented, information-oriented, intelligence-oriented and combination-oriented corresponding train development direction. Proposed method has a merit to prevent cross by mixed operation with existing system and improvement after validity demonstration and system development and supply train system to meet user requirement in exporting.

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