• Plant Biotechnology Reports
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• 제5권2호
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• pp.177-186
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• 2011

This study describes for the first time in Pinus genus a plant regeneration system via a combined pathway of somatic embryogenesis and organogenesis from immature seeds of radiata pine. Somatic embryos were obtained from embryogenic line 2162 of Pinus radiata D. Don on EDM basal medium containing $60{\mu}M$ ABA and 6% sucrose. The explants used for organogenesis experiments were either freshly collected somatic embryos or somatic embryos germinated for 1 week. Germination medium was half-strength LP medium, supplemented with 0.2% activated charcoal. Different induction periods and BA concentrations were assayed for shoot induction. After induction treatments, explants were elongated on the same medium used for germination stage. Rooting medium was quarter-strength LP medium supplemented with three different auxin treatments: $1.5mg\;L^{-1}$ 1-naphthalene acetic acid (NAA), $1.5mg\;L^{-1}$ indole-3-butyric acid (IBA) and $1mg\;L^{-1}$ IBA with $0.5mg\;L^{-1}$ NAA (MIX). The effect of the photon flux ($120mmol\;m^{-2}\;s^{-1}$ and darkness) in the first week of the explants in the rooting media was also tested. This methodology could offer an alternative to overcome some problems associated with somatic embryogenesis such as the seasonality of embryogenic tissue (ET) initiation or a low embryo production from the ET, a particularly important issue in the case of genetically transformed ETs.

• Journal of Forest and Environmental Science
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• 제29권4호
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• pp.275-281
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• 2013

To establish in vitro nodal culture conditions of Echinosophora koreensis Nakai, one of rare and endangered species famous for beautiful flowers in the Korean Peninsula, the influence of plant growth regulators (PGRs) on shooting and rooting from in vitro shoots was investigated. In shoot multiplication, addition of 6-benzylaminopurine (BA) to the half-strength Driver and Kuniyuki's media in the range of 2.22 to 8.88 ${\mu}M $induced 2.5 to 2.7 shoots per axillary bud; and addition of 2.27 ${\mu}M $ thidiazuron (TDZ) produced 3.2 shoots, during 4 weeks of culture, while zeatin and isopentenyl adenine (2ip) were not effective on shoot multiplication as observed from several combination treatments of BA with other PGRs. Shoots established were smaller than 2 cm in length, in most of the treatments. while in BA 8.88 ${\mu}M $ treatment more than 30% of shoots were longer than 2 cm and shorter than 4 cm. In rooting, naphthalene acetic acid (NAA) from 5.37 to 21.48 ${\mu}M $ showed the rooting rate from 40.0 to 62.5%. Indole butyric acid (IBA) addition had little effect on rooting (<10%), although some roots in IBA-containing media were longer than those in NAA. Micropropagation from axillary buds of nodular explants was applicable and promising to multiplication and conservation of Echinosophora koreensis Nakai.

• Journal of Plant Biotechnology
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• 제48권1호
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• pp.54-61
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• 2021

This work describe an efficient method for the shoot induction and plant regeneration of seedling-derived apical bud explants of Tilia mandshurica Rupr. & Maxim. The highest rate of shoot induction (82.2%) was obtained when apical bud explants from juvenile seedlings (5 months old) were cultured on Murashige and Skoog (MS) medium containing 1.0 mg/L 6-benzylaminopurine (BAP). However, apical bud explants obtained from mature trees (12 years old) did not produce any shoots, even with BAP supplementation. Among the three cytokinins tested for shoot multiplication (BAP, zeatin, and kinetin), BAP was the most effective; the highest number of shoots per explant (2.1) was observed on MS medium supplemented with 1.0 mg/L BAP. In contrast, the longest average shoot length (3.0 cm) was observed after growth on MS medium with 2.0 mg/L zeatin. No multiplication occurred when apical bud explants were cultured with kinetin-supplemented media. During rooting of in vitro-elongated shoots, the highest rooting rate (100%) was observed in half-strength MS medium supplemented with 0.5 ~ 1.0 mg/L indole-3-butyric acid (IBA) or 3.0 mg/L 1-naphthaleneacetic acid (NAA). During the acclimatization process, plantlets that were rooted on the IBA (0.5 mg/L)-supplemented medium had the highest survival rate (100%) and maximum root length (18.5 cm). These findings suggest that a low concentration (0.5 mg/L) of IBA is appropriate for the rooting and acclimatization of T. mandshurica. Plants were successfully transferred to the greenhouse with a 100% survival rate. This protocol will be useful for the large-scale propagation of Tilia species.

• 생물환경조절학회지
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• 제29권1호
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• pp.80-88
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• 2020

본 연구는 시설 딸기(Fragaria × ananassa Duch. cv. Maehyang)의 생육과 런너 생산을 위한 옥신계 식물 생장조절제인, IBA의 처리방법 및 농도에 따른 효과를 알아보기 위해 수행되었다. 딸기 묘는 2019년 4월 12일에 코이어 배지가 충진된 화분(150×135 ×90nm)에 정식 하였다. IBA는 0, 50, 100, 150, 200 mg·L^-1농도로 각각 엽면살포 및 배지관주 처리로 식물체당 50mL씩 처리하였다. IBA 처리는 2019년 4월 29일에 시작하였다. IBA의 엽면살포 및 배지관주 처리는 처리 시작일로부터 2주 간격으로 9주간 처리하였다. 처리 9주 후, 모주의 엽병장은 대조구에서 가장 짧았다. 자묘의 수는 엽면살포처리에서 감소하는 경향을 보였다. 측아의 수는 IBA 처리에서 감소하는 경향을 보였으며, 엽면살포 100 mg·L^-1처리에서 가장 적었다. 1차 묘와 2차 묘의 생체중과 건물중은 처리 간 유의적인 차이가 없었다. 하지만, 3차 묘에서 생체중과 건물중은 배지관주 100 mg·L^-1처리에서 가장 무거웠다. 그러므로, 3차 묘의 생육과 측아 발생 억제를 고려하였을 때, 배지관주 100 mg·L^-1처리가 IBA를 딸기에 처리함에 있어, 3차 묘의 생육과 자묘 생산에 적절한 처리방법 및 농도인 것으로 보이며 위 결과는 딸기 육묘기에 노동력 절감 및 묘소질 향상을 위한 식물생장조절제 활용의 기초자료로서 활용할 수 있을 것으로 판단된다.

• 한국자원식물학회지
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• 제35권6호
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• pp.770-777
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• 2022

The main objective of this study was to identify the most appropriate condition for root formation of in vitro micropropagated pear (Pyrus spp.) plants. In vitro propagation was induced on Murashige and Skoog (MS) medium with 2.0 mg/L of N6-benzyladenine (BA) and 0.2 mg/L of Indole-3-butyric acid (IBA) medium. The short pre-treatment of explants with a high concentration (1 mg/L) of NAA and IBA (R0 medium) in dark for three days, followed by transfer to five different media (R1 to R5) resulted in good rooting responses in the pear 'Oharabani (P. pyrifolia × P. communis)' genotype. For the rooting experiments, the highest rooting percentage (83.3 ± 8.3%), average root length (3.6 ± 1.9 mm), total root number (31 ± 4.0), and average root number per plant (2.6 ± 2.1) were obtained on half strength (1/2) of MS medium supplemented with 30 g/L sucrose without hormones and activated charcoal (AC) (R1 medium). The highest rooting percentage was obtained at 83.3% from explants on R1 and R3 media. The rooting procedure described in this study resulted in good root formation and significantly shorting the root induction time to within 14 days of culture. Further studies are underway to test the suitability of the protocol developed in this study for other pear genotypes.

• Plant Biotechnology Reports
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• 제4권2호
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• pp.109-116
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• 2010

Morinda citrifolia adventitious roots were cultured in shake flasks using Murashige and Skoog medium with different types and concentrations of auxin and cytokinin. Root (fresh weight and dry weight) accumulation was enhanced at 5 $mg\;l^{-1}$ indole butyric acid (IBA) and at 7 and 9 $mg\;l^{-1}$ naphthalene acetic acid (NAA). On the other hand, 9 $mg\;l^{-1}$ NAA decreased the anthraquinone, phenolic and flavonoid contents more severely than 9 $mg\;l^{-1}$ IBA. When adventitious roots were treated with kinetin (0.1, 0.3 and 0.5 $mg\;l^{-1}$) and thidiazuron (TDZ; 0.1, 0.3 and 0.5 $mg\;l^{-1}$) in combination with 5 $mg\;l^{-1}$ IBA, fresh weight and dry weight decreased but secondary metabolite content increased. The secondary metabolite content (including 1,1-diphenyl-2-picrylhydrazyl activity) increased more in TDZ-treated than in kinetin-treated roots. Antioxidative enzymes such as catalase (CAT) and guaiacol peroxidase (G-POD), which play important roles in plant defense, also increased. A strong decrease in ascorbate peroxidase activity resulted in a high accumulation of hydrogen peroxide. This indicates that adventitious roots can grow under stress conditions with induced CAT and G-POD activities and higher accumulations of secondary metabolites. These results suggest that 5 $mg\;l^{-1}$ IBA supplementation is useful for growth and secondary metabolite production in adventitious roots of M. citrifolia.

• 생물환경조절학회지
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• 제29권3호
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• pp.252-258
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• 2020

본 연구는 국내 '설향' 딸기 품종의 삽목 육묘 시 초기 활착을 증진시키고 묘소질을 향상시키기 위한 삽수 채취시기와 오옥신계 식물생장조절제 IBA, NAA의 적정 처리농도 구명을 위하여 수행되었다. 딸기 삽수는 6월 7일과 7월 5일의 두 차례에 걸쳐 채취했으며, IBA와 NAA는 각각 0.025, 0.05, 0.1%의 농도로 침지 처리하였다. 삽목한 묘는 터널재배 후 18일 동안 6회에 걸쳐 생존율을 조사하였다. 삽수 채취시기와 관계없이 NAA 처리에서는 묘 고사율이 높아, 딸기 삽목 육묘 시에는 NAA 처리가 부적합하였다. 삽수 채취시기와 식물생장조절제가 묘 생육에 미치는 영향을 조사한 결과 6월 채취묘는 IBA 0.1%에서, 7월 채취묘는 IBA 0.05%에서 묘의 활력이 우수하였다. 광합성 효율을 조사한 결과 6월 채취묘는 IBA 0.1%에서, 7월 채취묘는 IBA 0.05%에서 양자수율이 높았다. 따라서, 딸기 삽목묘의 생육을 증진시키기 위해서는 NAA보다 IBA를 사용하는 것이 적절하며, 이때 6월 삽수 채취 시에는 0.1%, 7월 삽수 채취 시에는 0.05%의 농도로 이용하는 것이 보다 적합한 것으로 판단되었다.

• 한국약용작물학회지
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• 제6권4호
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• pp.251-257
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• 1998

우리나라 황칠나무의 자생지인 전남 완도지방에서 황칠나무의 재배시 삽목번식법을 체계적으로 확립 하고자 시험을 실시한 결과는 다음과 같다. 1. 삽목의 종류로는 숙지삽과 녹지삽이 가능하였으며, 숙지삽 보다는 녹지삽이 캘러스형성이 양호하고 발근율이 높은 경향이었다. 삽목시기는 숙지삽은 2월${\sim}$3월 중순경 실시하고, 녹지삽은 2월${\sim}$3월경 실시하는 것이 발근율이 높아 적당한 시기로 판단된다. 2. 황칠나무의 경삽시 삽식형태는 관삽(normalcutting)보다는 단자삽(earthen-ball cutting) 이 캘러스 형성율이 높고 발근이 양호하였다. 3. 상토는 통기성과 보수성이 양호한 사양토가 발근에 효과적이었으며, 경제성이 있을 것으로 생각되어 적당한 상토로 생각된다. 4. 식물생장조절제는 IBA(indole butyric acid)를 100ppm처리할 경우 캘러스 형성율이 높고 발근이 촉진되는 경향이었다.

• 농업생명과학연구
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• 제43권6호
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• pp.59-65
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• 2009

Combinations of plant growth regulators, darkness treatments, and the order of expanding leaves for explants were evaluated for optimizing in vitro shoot regeneration rate of 'Whangkeumbae' pear. In a MS medium, supplemented with $8.88{\mu}M$ 6-benzylaminopurine (BA) and $0.49{\mu}M$ indole-3-butyric acid (IBA), green foci were observed on the surface of the callus 8 days after culture initiation. Some adventitious buds were later induced from those green foci, resulting in the appearance of normal shoots. In a medium containing $22.20{\mu}M\;BA$, the surface of the callus became compact and greenish, and many adventitious buds were formed over the entire area of the callus surface. When comparing BA concentration via histological observation, the section which had been treated with $22.20{\mu}M\;BA$ exhibited closer cell aggregation than those with $8.88{\mu}M\;BA$. The darkness treatment enhanced the formation of adventitious shoots for up to 3 weeks. The youngest two expanding leaves, proximal to the shoot apex, were proved to be the most regenerative, and yielded the highest shoot number per regenerating leaf. A fourth strength MS medium, which was supplemented with $0.54{\mu}M\;NAA$, yielded good quality plantlets, with regard to root number and root length.

• Journal of Ecology and Environment
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• 제46권3호
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• pp.218-242
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• 2022

Background: Promising specific growth regulators are employed in the tissue cultures of various bamboo species. Specific natural hardening mixtures support the acclimatization and adaptation of bamboo under protected cultivation. Results: The growth regulators like 2, 4-Dichlorophenoxyacetic acid (2, 4-D), Naphthaleneacetic Acid (NAA), Thidiazuron (TDZ), 6-Benzylaminopurine (BAP), Kinetin, Gelrite, Benzyl Adenine (BA), Indole Butyric Acid (IBA), Coumarin, Putrescine, Gibberellic acid (GA₃), Indole Acetic Acid (IAA) has been widely used for callus induction, root regeneration and imposing plant regeneration in various species of bamboo such as Bambusa spp. and Dendrocalamus spp. Different combinations of growth regulators and phytohormones have been used for regenerating some of the major bamboo species. Natural hardening materials such as cocopeat, vermicompost, perlite, cow dung, farmyard manure, compost, soil, garden soil, and humus soil have been recommended for the acclimatization and adaptation of bamboo species. Standard combinations of growth regulators and hardening mixtures have imposed tissue culture, acclimatization, and adaptation in major bamboo species. Conclusions: Bamboo contributes to soil fertility improvement and stabilization of the environment. Bamboo species are also involved in managing the biogeochemical cycle and have immense potential for carbon sequestration and human use. This paper aims to review the various growth regulators, natural mixtures, and defined media involved in regenerating major bamboo species through in vitro propagation. In addition, the ecological benefits of safeguarding the environment are also briefly discussed.