• Journal of The Korean Society of Grassland and Forage Science
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• v.27 no.4
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• pp.229-234
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• 2007

To select the most proper soil for root initiation from stem cuts of Birdsfoot trefoil (Lotus corniculatus L.), eight-week-old stem cuts were cultured on three types of soil [commercial bed soil, decomposed granite (DCG), and river sand] for one month. The results showed that the root initiation ratios on DCG (77.8%) and river sand (70.0%) were relatively high, but the ratio on commercial bed soil (41.1%) was very low. To examine the effect of rare earth (RE) and Indole-3-Butyric Acid (IBA) on root initiation from stem cuts of Birdsfoot Trefoil, stem cuts were cultured on two types of soil (DCG and river sand) with treatment of RE and IBA for one month. The root initiation ratios turned out to be 90.0% (DCG with 60 ppm of RE), 80.0% (river sand with 20 ppm of RE), 96.7% (DCG with 40 ppm of IBA), and 96.7% (river sand with 40 ppm of IBA). These results suggested that the most efficient way for root initiation of Birdsfoot trefoil was to culture the stem cuts on river sand or DCG over 30 days with IBA treatment (40 ppm).

• Plant Biotechnology Reports
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• v.5 no.1
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• pp.35-43
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• 2011

This communication describes for the first time an efficient and reproducible protocol for large-scale multiplication of Bambusa nutans. Nodal segments collected from field-grown clumps and cultured on Murashige and Skoog (MS) medium supplemented with $4.4{\mu}M$ benzylaminopurine (BA) and $2.32{\mu}M$ kinetin (Kin) gelled with 0.2% gelrite yielded 80% aseptic cultures with 100% bud-break. The in vitro-formed shoots obtained after bud-break were successfully multiplied in MS liquid medium supplemented with $13.2{\mu}M$ BA, $2.32{\mu}M$ Kin, and $0.98{\mu}M$ indole-3-butyric acid (IBA). Sub-culturing of shoots every 3 weeks on fresh multiplication medium yielded a consistent proliferation rate of 3.5-fold. Shoot clusters containing three to five shoots were successfully rooted with 100% success on half-strength MS liquid medium supplemented with $9.8{\mu}M$ IBA, $2.85{\mu}M$ indole-3-acetic acid (IAA), $2.68{\mu}M$ naphthaleneacetic acid (NAA), and 3% sucrose. Plantlets grown in vitro were acclimatized and subsequently transferred to the field. Inter-simple sequence repeat analysis has confirmed the genetic uniformity of the tissue-cultured plants up to 27 passages.

• Journal of Plant Biotechnology
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• v.5 no.2
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• pp.107-113
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• 2003

The effect of sodium sulphate on shoot induction and multiple shoot formation from nodal explants of Vitex negundo L. was tested on Murashige and Skoog's (MS) medium fortified with different auxins, cytokinins and sucrose. Highest percentage $(97.78\%)$ of explants for shoot induction and multiple shoot (20.68/explant) production were observed in the combination treatment of $N^6-Benzyl$ adenine (BA) $(17.80\;{\mu}M/L)$, ${\alpha}-Naphthalene$ acetic acid (NAA) $(2.15\;{\mu}M/L)$ and $5\%$ sucrose supplemented with 100 mg/L sodium sulphate. In vitro raised shoots were rooted on the half-strength MS medium fortified with different concentrations of NAA, Indole-3-acetic acid (IAA), and Indole-3-butyric acid (IBA) alone and in combinations. Among the treatments, $4.90\;{\mu}M/L$ of IBA was found most effective $(95.56\%)$ in inducing roots. The rooted plantlets were shifted to glasshouse for acclimatization and later transferred to the field with cent percent survival. Furthermore, in vitro flowering was observed in the shoots cultured on MS medium supplemented with BA $(8.90\;{mu}M/L)$ and NAA $(1.61\;{\mu}M/L)$.

• Food Science and Preservation
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• v.16 no.4
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• pp.459-462
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• 2009

'Fuyu' (Diospyros kaki L.) is an important sweet persimmon cultivar, and the fruits are often stored in a modified atmosphere after harvesting in South Korea. However, blossom-end browning and darkening of fruit often occur after harvest or during storage, which decreases fruit quality in the fresh fruit market. High fruit calcium concentration would reduce oxidation of phenolic compounds in the cytoplasm such oxidation is responsible for fruit browning. This study investigated the effects of soluble calcium fertilization and foliar application, and indole-3-butyric acid (IBA) fertilization on fruit quality and browning. Trees received one of the following five treatments: 1) control (no calcium or IBA); 2) calcium fertilization (Ca FG, 2 mL per tree); 3) calcium foliar application (Ca FA, 2 mL); 4) calcium and IBA fertilization (Ca+IBA) 5) IBA fertilization (IBA, 2 mL. Fruit calcium concentration was highest in trees treated by Ca FA, and lowest in control trees. Generally, fruit calcium concentration was high in the stem end but low in the blossom end, which usually first develops fruit-browning symptoms. There were no apparent differences in fruit qualities such as firmness, soluble solid content, and weight among treatments. Fruit browning occurred at frequencies of about 14%, 20%, and 50% on Ca FA, Ca FG, and control trees, respectively. Therefore, the improved fruit calcium level seen when trees received Ca or IBA application tended to prevent fruit browning, which increased fruit quality and storage properties.

• Journal of Plant Biotechnology
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• v.6 no.3
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• pp.181-188
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• 2004

Zingiber officinale buds from the rhizomes were used to produce in vitro shoots. These explants produced the largest number of multiple shoots, 9.8 shoots per explant, when were cultured on MS (Murashige and Skoog 1962) medium supplemented with 2.0 mg/L benzyladenine (BA) and 2.0 mg/L indole butyric acid (IBA). This medium was also found to be suitable for in vitro propagation of other Zingiberaceae species: Alpinia conchigera, Alpinia galanga, Curcuma domestica, C. zedoaria and Kaempferia galanga. Both C. domestica and C. zedoaria produced more multiple shoots when were cultured in the liquid proliferation medium, MS medium containing 2.0 mg/L BA and 2.0 mg/L IBA. To maintain the in vitro plantlets of Zingiberaceae species, they were required to subculture every four weeks. After executing proper acclimatization protocol, in vitro plantlets of Alpinia galanga, A. conchigera, Curcuma domestica, C. zedoaria, Kaempferia galanga and Zingiber officinale could be successfully planted in the field with high percentage of survival.

• Journal of Forest and Environmental Science
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• v.30 no.2
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• pp.218-225
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• 2014

Studies were carried out to standardize and develop a suitable macro-propagation technology for large scale production of superior clonal stock through stem cuttings in Commiphora wightii Arnott (Bhandari), a data deficient medicinal plant of arid region. For the purpose, three experiments were conducted. The first experiment was tried to elucidate the impact of various cutting diameters (0.50-0.75 cm, 0.75-1.00 cm, 1.00-1.50 cm, and >1.50 cm) in combination with varying growing conditions (sunlight, shade house and mist chamber) on shoot sprouting and rooting without using exogenous plant growth regulators. Cutting diameter (size 0.75-1.00 cm) in mist chamber has shown maximum sprouting (90.00%) and rooting (73.33%), primary root (6.67) and secondary root (16.67) followed by 1.00-1.51 cm in mist chamber. Minimum sprouting (40.00%), rooting (33.33%), number of shoot (1.33), primary root (1.00) and number of secondary root (1.00) was recorded in cutting diameter (size >1.50 cm) in sunlight. Second experiment was performed to find out optimum growth regulator concentration of rooting hormone (100, 200, 500 and 1000 ppm) of Indole-3-acetic acid (IAA) and Indole-3-butyric Acid (IBA) on adventitious root formation on cuttings diameter (size 0.25-0.50 cm) in comparison to control. Maximum rooting percentage (93.33%) was recorded in 200 ppm followed by 500 ppm (86.66%) of IBA as compared to control, which showed only 60 per cent sprouting. Third experiment was performed with newly formed juvenile micro-cuttings treated with varying concentrations of IAA and IBA. The juvenile cuttings (size 6-10 cm, basal dia <0.25 cm) were selected as micro-cuttings. The cuttings treated with IBA (500 ppm) showed 64.30% rooting as compared to other treatments. Results of above experiments indicate that cuttings (size 0.75-1.00 cm dia) may be developed in mist chamber for better performance. While using heavier cuttings, no growth promoting hormones is required however; growth regulator 200 ppm concentration of IBA rooting hormone was observed optimum for promoting macro-propagation in stem cuttings of lower diameter class (0.25-0.50 cm).

• Korean Journal of Medicinal Crop Science
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• v.14 no.2
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• pp.92-95
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• 2006

A method for plant regeneration via organogenesis from Pelagonium inquinans leaf disc has been developed. Mature leaf explants were collected from field-grown plants and used for the induction of adventitious shoot regeneration on Murashige and Skoog (MS) medium supplemented with 3% (w/v) sucrose plus plant growth regulators. Maximum shoot organogenesis, with $11.8{\pm}1.5$ shoots (98.6%) per leaf disc, was obtained with $2\;mg/l$ $N^6-benzyladenine$ (BA) and $0.5\;mg/l$ ${\alpha}-naphthyleneacetic$ acid (NAA) in 30 days. For rooting, the in vitro proliferated and elongated shoots were excised into 1.5-2 cm in length microcutting, which were plated individually on an half-strength MS (1/2MS) medium supplemented with 2% (w/v) sucrose plus various concentrations of indole-3-butyric acid (IBA). Shoots rooted with a frequency of 100% following culture on 1/2MS medium containing $0.5\;mg/l$ IBA.

• Korean Journal of Plant Resources
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• v.22 no.4
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• pp.281-286
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• 2009

Rhodiola sachalinensis is one of the most popular oriental medicines in East Asia. It is a perennial herb, belonging to the family Crassulaceae, which is mainly distributed in mountains at the altitudes of 1700-2500 m in Baek-Du mountain. Cultivation of this species for the production of medicine materials is not easy in nature, because of restrict habitats. In vitro production of roots can be applicable for the production of medicinal materials. Here, we investigated the optimal conditions for induction and proliferation of adventitious roots in in vitro culture system. Leaf, stem and root segments from R. sachalinensis were cultured on Murashige and Skoog(MS) medium supplemented with the various concentrations of IBA(Indole-3-butyric acid)(0.5, 1.0, 3.0, and 5.0 mg/L) and sucrose(10, 30, 50, and 100 g/L). The optimal explant for adventitious root induction was leaf segment. Induction of adventitious roots was highest on MS medium supplemented with 5.0 mg/L IBA and sucrose 30 g/L. In liquid culture, fresh weight of adventitious roots was highest(15.65 g) on 1/3 strength MS liquid medium supplemented with 5.0 mg/L IBA with 30 g/L sucrose. These results revealed the first attempt for the production of adventitious roots in R. sachalinensis.

• Horticultural Science & Technology
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• v.28 no.4
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• pp.678-684
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• 2010

In an attempt to improve biomass and bioactive compound production, we cultured adventitious roots of $Eleutherococcus$ $koreanum$ in 250 mL Erlenmeyer flasks using Murashige and Skoog (MS) medium with different concentrations of auxins (IBA, NAA, IAA) and cytokinins (BA, kinetin, TDZ). Root biomass (fresh and dry weight) was enhanced at $5mg{\cdot}L^{-1}$ indole-3-butyric acid (IBA) after 5 weeks of culture. The content of total phenolics and flavonoids was also increased with $5mg{\cdot}L^{-1}$ IBA compared to ${\alpha}$-naphtalene acetic acid (NAA) or indole-3-acetic acid (IAA) treatments. The combination of $5mg{\cdot}L^{-1}$ IBA with $0.1mg{\cdot}L^{-1}$ thidiazuron (TDZ; N-phenyl-N'-1,2,3,-thidiazol-5-ylurea) enhanced root fresh and dry weight (1.4- and 1.6-fold, respectively) as well as the content of total phenolics and flavonoids compared to the relative control (without cytokinin). On the contrary, $N_6$-benzyladenine (BA) and 6-furfurylaminopurine (kinetin) did not significantly affect root biomass and bioactive compound production in adventitious roots of $E.$ $koreanum$. These results suggested that $5mg{\cdot}L^{-1}$ IBA combination with $0.1mg{\cdot}L^{-1}$ TDZ supplementation was most suitable for both biomass and bioactive compound production from adventitious roots of $E.$ $koreanum$.

• Journal of Plant Biotechnology
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• v.35 no.3
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• pp.209-213
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• 2008

This study was conducted to regenerate shoots from internode sections(about 1mm in thickness) of Ardicia pusilla de Candolle. Internode sections were cultured on MS medium supplemented with TDZ or both TDZ and IBA. At one month after culture, primodium, which looks like protocorm like body(PLB) of orchid, appeared around swollen internodes. And then it grew and changed into the shape similar to granule of orange at two or three months after culture. At four to five months after culture, explants covered with them became a cluster, and then multiple shoots were regenerated from them. Primodia formation was the best when internode was cultured on MS medium supplemented with 0.25 $mg{\cdot}L^{-1}$ thidiazuron(TDZ) and 0.5 $mg{\cdot}L^{-1}$ indole-3-butyric acid(IBA). That internodes were cultured on MS medium supplemented with either higher concentration of TDZ than that of IBA, or equal concentration of TDZ and IBA, or TDZ only was little effective for primodia formation.