Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지
DOI QR코드

DOI QR Code

Shoot regeneration from internode sections of Ardisia pusilla DC.

  • Lee, Su-Young (Dept. of Horticultural Biotechnology, National Horticultural Research Institute, Rural Development Administration) ;
  • Kim, Young-Soon (Dept. of Horticultural Biotechnology, National Horticultural Research Institute, Rural Development Administration) ;
  • Han, Bong-Hee (Dept. of Horticultural Biotechnology, National Horticultural Research Institute, Rural Development Administration)
  • Published : 2008.09.30
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to regenerate shoots from internode sections(about 1mm in thickness) of Ardicia pusilla de Candolle. Internode sections were cultured on MS medium supplemented with TDZ or both TDZ and IBA. At one month after culture, primodium, which looks like protocorm like body(PLB) of orchid, appeared around swollen internodes. And then it grew and changed into the shape similar to granule of orange at two or three months after culture. At four to five months after culture, explants covered with them became a cluster, and then multiple shoots were regenerated from them. Primodia formation was the best when internode was cultured on MS medium supplemented with 0.25 $mg{\cdot}L^{-1}$ thidiazuron(TDZ) and 0.5 $mg{\cdot}L^{-1}$ indole-3-butyric acid(IBA). That internodes were cultured on MS medium supplemented with either higher concentration of TDZ than that of IBA, or equal concentration of TDZ and IBA, or TDZ only was little effective for primodia formation.

Keywords

References

  1. Achkor H, Diaz M, Fernάndez MR, Biosca JA, Pares X, Martinez MC (2003) Enhanced formaldehyde detoxification by overexpression of glutathione-dependent formaldehyde dehydrogenase from Arabidopsis. Plant Physiol 132: 2248-2255 https://doi.org/10.1104/pp.103.022277
  2. Burdyn L, Luna C, Tarrago J, Sanseberro P, Dudtt N, Gonzάlez A, Mroginski L (2006) Direct shoot regeneration from leaf and internode explants of Aloysia polystachya [gris.] Mold. (Verbenaceae). In Vitro Cell Dev Biol_Plant 42: 235-239 https://doi.org/10.1079/IVP2006751
  3. Cuenca B, Ballester A, Vieitez AM (2000) In vitro adventitious bud regeneration from internode segments of beech. Plant Cell Tissue and Culture 62: 213-220
  4. Goo DH, Kwon OK, Lee YR, Huh EJ (2008) Micropropagation of Ardisia pusilla and Ardisia Japonica in vitro. Acta Hort 766: 237-241
  5. Kang GH, Oh OS, Goo DH, Eun JS, Kim HM (2005) In vitro mass propagation of Ardisia pusilla DC. J Plant Biotechnol 32: 281-285 https://doi.org/10.5010/JPB.2005.32.4.281
  6. Kelkar SM, Krishnamurthy KV (1998) Adventitious shoot regeneration from root, internode, petiole and leaf explants of Piper colubrinum Link. Plant Cell Reports 17: 721-725 https://doi.org/10.1007/s002990050472
  7. Kim, KJ (2007) Air filtration using Korean native plant. The tenth anniversary symposium of foundation of Korean wild plant research. pp 15-31
  8. Kulkarni, AA, Thengane SR, Krishnamurthy KV (2000) Direct shoot regeneration from node, internode, hypocotylsand embryo explants of Withania somnifera. Plant Cell Tissue and Culture 62: 203-209 https://doi.org/10.1023/A:1006413523677
  9. Lee CH (2006) The use of 6-benzylaminopurine for lateral branching of Ardisia pusilla. J Kor Soc Hort Sci 46: 396-401
  10. Lin CC (1986) In-vitro culture of flower stalk internodes of phalaenopsis and doritaenopsis. Lindleyana 1: 158-163
  11. Lu CY (1993) The use of thidiazuron in tissue culture. In vitro Cell Dev Biol 29: 92-96 https://doi.org/10.1007/BF02632259
  12. Luft JH (1973) Compounding of Luft's epon embedding medium for use in electron microscopy with reference to anhydride: Epoxide ratio adjustment Mikroskopie 29: 337-342
  13. Matt A, Jehle JA (2005) In vitro plant regeneration from leaves and internode sections of sweet cherry cultivars (Prunus avium L.). Plant Cell Rep 24: 468-476 https://doi.org/10.1007/s00299-005-0964-6
  14. Ministy of Agr. & For. 2006. Flower production in Korea
  15. Poovaiah CR, Weller SC, Jenks MA (2006) In vitro adventitious shoot regeneration of native spearmint using intermodal explants. Hortscience 41: 414-417
  16. Sanikhani M, Frello S, Serek M (2006) TDZ induces shoot regeneration in various Kalanchoe blossfeldiana Poelln. cultivars in the absence of auxin. Plant Cell Tissue and Organ Culture 85: 75-82 https://doi.org/10.1007/s11240-005-9050-6