• 한국동물위생학회지
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• 제21권3호
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• pp.277-284
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• 1998

Serum samples were collected from 100 breeders and their progeny 600 broilers. The breeders and broilers were vaccinated against infectious bronchitis(IB), infectious bursal disease(IBD) and Newcastle disease(ND) viruses according to general vaccination program. The antibodies in serum samples against IB, IBD and ND viruses were detected by ELISA using commercial ELISA kit. Geometric mean titer(GMT) of ELISA was monitored from 1-day-old to 35-day-old broilers and compared to that of breeder chickens. The GMT of ELISA to IB, IBD and ND was declined half level of the day old broiler's antibody titers at about 4, 9 and 4 days of age. The GMT of ELISA to IB, IBD and ND was declined than that of protective antibody titer at about 12, 11, and 15 days of age. Thereafter, the GMT of ELISA to IB, ND were declined and disappeared according to age of broilers. The GMT of ELISA to IBD was declined according to age of broilers, but at 25 days of age increased and 31 days of age increased than that of protective antibody titer. Taken together, these studies led to conclusion that time-course of antibody titers of broilers from vaccinated breeders and that of progeny broliers which vaccinated according to vaccine program. Those are very important data to design vaccine program to breeders and broilers.

• 한국동물위생학회지
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• 제14권1호
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• pp.27-40
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• 1991

In order to investigate the biological properties, pathogenicity and immune responses in artficially infected SPF chickens with Avian infectious bronchitis virus that was isolated from chickens showing IB like signs in southern region of Chung buk. Results obtained throuth the experiments are summarized as follows. 1. From 15 IB suspected cases, two strains of IB virus were isolated, one each from the tracheas and lungs. 2. Infectious bronchitis specific embryo lesions were observed after four serial passages of the isolates in chicken embryos. 3. The field isolates and M-41 strain of IB virus interfered with the replication of Newcastle disease virus in chicken embryos. 4. When specific pathogen free chickens, two week old, were inoculated with the IB virus isolates, clinical respiratory signs as dyspnea, coughing were observed. Airsacculitis was observed by necropsy. 5. AGP antibody positive rates of inoculated SPF chickens were highest on day 14 and lowest on day 36, while HI antibody responses were detected on day 14 in all Groups, the reinoculated Group was shown highest titers. 6. By Indirect immunofluorescence antibody assay of artificially infected SPF chickens, the viral antigens were detected in tissues of larynx, trachea and lung on the 4 th to 7 th days post inoculation.

• 한국동물위생학회지
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• 제21권2호
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• pp.133-139
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• 1998

Serum samples collected from 30 breeders and their progeny 30 chicks. The antibodies against infectious bronchitis(IB), infectious bursal disease (IBD) and Newcastle disease(ND) viruses were detected by ELISA using commercial ELISA kit. The breeders were vaccinated against IB, IBD and ND viruses according to general vaccination program. Geometric mean titers(GMT) of ELISA were monitored from 1-day old to 17-day old chicks and compared with breeder chickens. The GMT of ELISA to IB, IBD and ND were declined half level of the breeder antibody titer at 6-, 8- and 7-day old. And, the GMT of ELISA to IB, IBD and ND were declined than that of protective titer at 6-, 1-, and 4-day old. Thereafter, the GMT of ELISA was declined and disappeared according to ages of chicks. Taken together, this study led to conclusion that time-course of maternal antibody titers of chicks from vaccinated breeders, and this is very important data for vaccination to chicks.

• 대한수의학회지
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• 제30권1호
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• pp.79-84
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• 1990

A single inoculation of combined vaccines of Newcastle disease and avian infectious bronchitis of chicken, in a form of gel-oil emulsion type (gel-OEV) was tested their immunogenecity in chickens. The results were summerized as follows: 1. Average minimum and maximum ELISA antibody titers of ND were recorded 2407 and 13144 respectively. In the case of IB, 1824 and 4496 were recorded as minimum and maximum titers. 2. The distribution of average proportional groups, in the lowest and the highest, were 1.6 and 7.0 in ND ELISA and 1.4 and 2.8 in IB ELISA antibody titers. 3. ND ELISA antibody titers were significantly increased upto 7th week after the vaccination. On the other hand, IB ELISA antibody titers were raised upto 4th week after the vaccination.

• 한국동물위생학회지
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• 제24권1호
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• pp.21-29
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• 2001

To compare of serum antibody titers using ELISA and HI, serum samples were collected from 100 breeders and their progeny 550 broilers. The breeders and broilers were vaccinated with infectious bronchitis(IB)- and Newcastle disease(ND)-viruses according to general vaccination program. The antibodies in serum samples against IB and ND viruses were detected by enzyme-linked immunosorbent assay(ELISA) using commercial ELISA kit and hemagglutination inhibition(HI) test. Geometric mean titer(GMT) of ELISA and In titers were monitored from 1-day-old to 35-day-old broilers and compared to those of breeder chickens. The antibody titers of breeders vaccinated with ]B virus showed 47,800, ELISA and 7.2, HI, respectively. Progeny chicks, 1-day-old, vaccinated with IBV showed high antibody titers than those of breed chickens. Those chicks were maintained protective antibody levels until 11-day-old. From 14-day-old, the antibody level decreased below protective levels. In ND, breeders serum antibody titers ELISA and Eiu were 30,200 GMT and 8.7 HI titer, respectively. On 1-day-old chicks, antibody levels was decreased to half in ELISA(16,270) compared with those of breeders, but In titers was 7.4. Progeny broilers, protective antibody level was maintained until 14- day-old by ELISA, but at 11-day-old by HI titers. After then, ND antibody titer was continuously decreased underdefense level. These result indicated that the ELISA method be more sensitive than HI titration to detect serum antibody level for IBV and NDV.

• Parasites, Hosts and Diseases
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• 제51권4호
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• pp.485-488
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• 2013

The performance values of available techniques used in serodiagnosis of toxoplasmosis are satisfactory but they raise problems of equivocal and discordant results for very low IgG titers. Recently marketed, LDBio-Toxo II IgG Western blot (IB) showed an excellent correlation with the dye test. We estimated the proportion of equivocal and discordant results between the enzyme immunoassay Platelia Toxo IgG (EIA-IgG) and fluorescent antibody test (FAT) and assessed the usefulness of the IB as a confirmatory test. Out of 2,136 sera collected from pregnant women, 1,644 (77.0%) tested unequivocally positive and 407 (19.0%) were negative in both EIA-IgG and FAT. The remaining 85 (4%) sera showed equivocal or discordant results. Among them, 73 (85.9%) were positive and 12 (14.1%) were negative in IB. Forty-one (89.1%) equivocal sera in EIA-IgG and 46 (86.8%) equivocal sera in FAT were positive in IB. Reducing the cut-off values of both screening techniques improved significantly their sensitivity in detecting very low IgG titers at the expense of their specificity. In conclusion, equivocal results in routine-used techniques and their discordance in determination of the immune status in pregnancy women were not uncommon. IB test appeard to be highly useful in these situations as a confirmatory technique.

• 한국가금학회:학술대회논문집
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• 한국가금학회 2003년도 제20차 정기총회 및 학술발표회
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• pp.75-76
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• 2003

육계사료내 마늘분말의 첨가 급여가 육계의 성장과 콜레스테롤 대사에 미치는 영향을 규명하기 위해 본 연구를 실시하였다. 1일령 육계에게 마늘분말을 첨가하지 않은 대조사료(항생제 첨가구 또는 무첨가구), 마늘분말을 1 ％, 3 ％ 및 5 ％ 수준으로 첨가한 실험사료를 6주간 급여하였다. 증체량은 항생제 무첨가 대조구에 비해 마늘분말 3 ％ 및 5 ％ 첨가구에서 유의하게 증가하였다. 모든 마늘분말 첨가구에서 조직내 콜레스테롤 함량은 다소 감소하는 경향을 나타내었으며, 간내 HMG-CoA reductase mRNA의 발현이 저하되는 결과가 관찰되었다. IB 항체 생성량은 항생제 무첨가 대조구에 비해 모든 마늘분말 첨가구에서 유의하게 증가하는 결과가 관찰되었다.

• 한국동물위생학회지
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• 제40권2호
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• pp.89-94
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• 2017

This study was conducted from May to December 2016 to investigate the prevalence of antibody titers for Newcastle disease (ND), Infectious Bronchitis (IB), Egg drop syndrome (EDS), Avian pneumovirus (APV), Fowl Typhoid (FT), Mycoplasma gallisepticum (MG), Mycoplasma synoviae (MS) in laying hens in Incheon area. In ND, 418 (99.5%) of the 420 outbreaks were positive. IB showed 330 (78.5%) positive antibody out of 420 cases. EDS showed 419 (99.7%) positive seropositive rates among 420 cases. In the case of APV, 357 cases (85%) were positive in 420 cases. In the case of PT, the positive rate of antibody was 128 (30.4%) out of 420 cases. Among the 420 cases of MG, 222 cases (52.8%) were positive for antibodies. In the case of MS, 395 out of 420 cases (94.0%) were positive. This result suggests that the positive rate of antibody for PT in Incheon area was low. And it was also clear that MS without vaccine is now rampant in Incheon.

• 한국가금학회지
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• 제46권4호
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• pp.235-247
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• 2019

본 연구에서는 국내 산란계의 주요 질병에 대한 면역상태 및 역가수준을 파악하기 위해 2015년부터 2017년까지 3년간 충북대 수의과대학 조류질병학 실험실에 의뢰된 산란계의 혈청을 성장기와 산란기를 고려하여 주령별로 구분하여 분석하였고, 계절에 따른 검사결과를 분석하였다. 검사대상 질병은 검사 의뢰건 수가 많고, 혈청검사 결과가 유의미한 질병인 LPAI, ND, IB, aMPV, EDS'76, IBD, CIA, MS로 한정하였다. AI와 ND의 경우 전체적인 GMT, CV의 양상은 비슷하게 진행되었다. 반면 LPAI의 경우 주령별 계군양성율이 불균일하고 편차가 최대 약 50%의 큰 차이를 보였지만 ND의 경우 균일한 양성률을 보이며 편차는 2% 미만으로 낮게 확인되었다. 또한, 전체적인 GMT 역시 LPAI가 ND에 비하여 낮게 형성됨을 확인하였다. 이는 각 질병의 특성과 백신접종의 차이에 따른 결과로 판단된다. IB는 음성계군과 전체계군을 비교할 때 전체 계군의 역가수준이 전반적으로 높았고, CV값은 낮은 경향을 확인할 수 있었으며, 이를 통해 야외 IB virus의 노출을 추정할 수 있었다. aMPV는 2011년에 백신 출시 이후 과거에 비해 높은 항체양성률과 GMT역가 상승을 확인할 수 있었다. EDS'76은 전주령 구간 균일한 역가분포를 나타내었고, 백신에 의한 역가형성을 확인할 수 있었다. IBD는 0~1.5주령의 어린 일령에서 전반적인 IBD 모체이행항체가 높은 수준의 양성률을 보였고 3주령 이후 높게 형성되는 양성률이 모체이행항체수준을 높이는 것으로 추정되고, 향후 지속적인 혈청학적 모니터링이 진행되어야 하겠다. MS는 2017년 백신 출시 이후 양성률과 역가가 높게 나타나는 결과를 보였지만 의뢰건 수가 적고 백신 도입 초기이기 때문에 지속적인 모니터링이 필요하다.

• 한국동물위생학회지
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• 제27권3호
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• pp.281-288
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• 2004

As all other intensively farmed domestic species, most mortality in ostriches is closely to rearing conditions. While ostriches is also highly sensitive to stress, species-specific infectious disease play only a minor role. But investigation of ostrich's disease is not peformed almost in Korea. The study was performed to investigate the titers of antibody for Newcastle disease(ND), Infectious bronchitis(IB), Egg drop syndrome '76(EDS), Avian influenza(AI), salmonellosis, Mycoplasma gallisepticum infection(MG), Mycoplasma synoviae infection(MS), Infectious bursal disease(IBD), Brucellosis, Toxoplasmosis, Japanese encephalitis(JE), Porcine parvovirus infection, Encephalomyocarditis and Porcine reproductive respiratory syndrome (PRRS). The results obtained in the 62 ostrich sera slaughtered in Gyeongbuk province were summarized as follows: The average of antibody positive rates to ND, IB, EDS, AI(H9Nl), JE, Porcine parvovirus infection and Encephalomyocarditis by HI test were $75.8\%,\;100\%,\;0\%,\;0\%,\;51.6\%,\;50\%\;and\;56.5\%$ respectively. The antibody positive rates to salmonellosis, MG, MS by plate agglutination test were $12.9\%,\;25.8\%,\;and\;0\%$ respectively. Antibodies to disease agent such as IBD and AI by agar gel precipitation(AGP) test, Brucellosis by tube agglutination, toxoplasmosis by latex agglutination test and PRRS by IFA were all negative.