• Title/Summary/Keyword: I-SSR

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Analysis of Genetic Diversity and Population Structure for Core Set of Waxy and Normal Maize Inbred Lines using SSR Markers (SSR 분자마커를 이용한 찰옥수수 및 종실용 옥수수 자식계통들의 핵심집단에 대한 유전적 다양성 및 집단구조 분석)

  • Sa, Kyu Jin;Kim, Jin-Ah;Park, Ki Jin;Park, Jong Yeol;Goh, Byeong Dae;Lee, Ju Kyong
    • Korean Journal of Breeding Science
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    • v.43 no.5
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    • pp.430-441
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    • 2011
  • Maize is divided into two types based on the starch composition of the endosperm in the seed, normal maize(or non-waxy maize) and waxy maize. In this study, genetic diversity and population structure were investigated among 80 waxy maize and normal inbred lines(40 waxy maize inbred lines and 40 normal maize inbred lines) using 50 SSR markers. A total of 242 alleles were identified at all the loci with an average of 4.84 and a range between 2 and 9 alleles per locus. The gene diversity values varied from 0.420 to 0.854 with an average of 0.654. The PIC values varied from 0.332 to 0.838 with an average of 0.602. To evaluate the population structure, STRUCTURE 2.2 program was employed to confirm genetic structure. The 80 waxy and normal maize inbred lines were separated with based on the membership probability threshold 0.8, and divided into groups I, II and admixed group. The 13 waxy maize inbred lines were assigned to group I. The 45 maize inbred lines including 7 waxy maize inbred lines and 38 normal maize inbred lines were assigned to group II. The 22 maize inbred lines with 20 waxy maize inbred lines and 2 normal maize inbred lines were contained in the admixed group. The cluster tree generated using the described SSR markers recognized three major groups at 31.7% genetic similarity. Group I included 40 waxy maize inbred lines and 11 normal maize inbred lines, and Group II included 27 normal maize inbred lines. Group III consist of only 2 normal maize inbred lines. The present study has demonstrated the utility of SSR analysis for the study of genetic diversity and the population structure among waxy and normal maize inbred lines. The information obtained from the present studies would be very useful for designing efficient maize breeding programs in Maize Experiment Station, Kangwon Agricultural Research and Extension Services.

Chloroplast genome of the conserved Aster altaicus var. uchiyamae B2015-0044 as genetic barcode

  • Lee, Minjee;Yi, Jae-Sun;Park, Jihye;Lee, Jungho
    • Journal of Species Research
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    • v.10 no.2
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    • pp.154-158
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    • 2021
  • An endemic endangered species, Aster altaicus var. uchiyamae (Danyang aster) B2015-0044, is cultivated at the Shingu Botanical Garden, which serves as the ex situ conservation institution for this species. In this work, we sequenced the chloroplast genome of A. altaicus var. uchiyamae B2015-0044. We found that the chloroplast (cp) genome of B2015-0044 was 152,457 base pairs(bps) in size: 84,247 bps of large single copy regions(LSC), 25,007 bps of inverted repeats(IRs), and 18,196 bps of small single copy regions. The B2015-0044 cp genome contains 79 protein-coding genes (PCGs), 4 RNA genes, 29 tRNA genes, and 3 pseudogenes. These results were identical to a previously reported cp genome (Park et al., 2017), except for two sites in introns and three in intergenic spacer (IGS) regions. For the intronic differences, we found that clpP.i1 had a 1-bp small simple repeat (SSR) (T) and petD.i had a 3-bp SSR (ATT). We found 1-bp SSRs in the IGSs of trnT_ggu~psbD and psbZ~trnG_gcc, C and A, respectively. The IGS of(ndhF)~rpl32 had a SNP. Based on our results, the cp genome of the A. altaicus var. uchiyamae can be classified into two genotypes, [C]1-[A]12-[T]12-[ATT]4-C and [C]2-[A]11-[T]11-[ATT]2-A.

On the Performance of Turbo Codes-Based Hybrid ARQ with Segment Selective Repeat in WCDMA

  • Shi Tao;Cao Lei
    • Journal of Communications and Networks
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    • v.8 no.2
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    • pp.212-219
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    • 2006
  • In this paper, a new turbo codes-based hybrid automatic repeat request (TC-HARQ) scheme with segment selective repeat (SSR) is proposed. The main strategy is, upon retransmission, to repeat the data that are most important for the next round of decoding based on the distribution of residual errors after current decoding. The performance in terms of reliability and throughput is analyzed. To adapt to correlated fading channels where an inter-leaver is always employed before transmission, we further modify the SSR strategy so that data having experienced correlated deep fading are selected for retransmission. Finally, this proposed scheme is applied to the wideband code division multiple access (WCDMA) system under frequency selective fading channels. Simulation results demonstrate that in all single and multiple user cases, SSR-based TC-HARQ leads to significant throughput improvement with similar bit error rate (BER) performance as compared to type-I TC-HARQ.

Genetic Diversity and Spatial Genetic Structure of Populus koreana Population in Mt. Odae, Korea (오대산 물황철나무(Populus koreana) 집단의 유전다양성 및 공간적 유전구조 분석)

  • Shin, Sookyung;Song, Jeong-Ho;Lim, Hyo-In;Jang, Kyung-Hwan;Hong, Kyung-Nak;Lee, Jei-Wan
    • Journal of Korean Society of Forest Science
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    • v.103 no.1
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    • pp.59-64
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    • 2014
  • This study describes analysis of genetic diversity and spatial genetic structure of Korean poplar (Populus koreana Rehder) in Mt. Odae using I-SSR markers. P. koreana is a deciduous broad-leaved tree species that primarily grows in the alpine valleys of China, Russia and North Korea. In South Korea, P. koreana is found limitedly in Gangwon province. Especially, the population in Mt. Odae is located on the southern limit line, its importance is emphasized from the genetic resource conservation perspective. The Shannon's diversity (I=0.230) and the expected heterozygosity (He=0.151) were relatively low as compared with those of other plant species. Spatial autocorrelation analysis using Tanimoto's distance showed that the genetic patch was founded within 400 m. It is suggested that individual trees for ex situ conservation should be sampled with a minimum distance of 400 m between trees.

Diversity and Geographical Relationships by SSR Marker in Subgenus Soja Originated from Korea (SSR 마커에 의한 한국 원산 Soja 아속의 다양성과 지리적 유연관계)

  • Cho Yang-Hee;Yoon Mun-Sup;Lee Jeong-Ran;Baek Hyung-Jin;Kim Chang-Yung;Kim Tae-San;Cho Eun-Gi;Lee Hee-Bong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.3
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    • pp.239-247
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    • 2006
  • This study was carried out to investigate polymorphism, gene diversity, and geographical relationships of 81 Korean wild (Glycine soja) and 130 cultivated soybeans (G. max) using seven simple sequence repeat (SSR) markers. A total of 144 alleles were observed in 211 accessions with an average of 20.6. Each SSR loci showed 13 (Satt532) to 41 (Sat_074) multialleles. The range of alleles within the loci was wider in wild soybean than the cultivated soybeans. The average genetic diversity values were 0.88 and 0.69 in wild and cultivated soybeans, respectively. In a scatter diagram of wild and cultivated soybeans based on canonical discriminant analysis, CAN1 accounted for 84.2% while CAN2 did 8.5%. Two species were grouped into three: group I (G. max), group II (G. soja), and group III (complex of G. max and G. soja). The geographical relationships of wild soybean were distinguished into two groups: Gyeonggi for Group I, and Gyeongsang, Jeolla, Gangwon, and Chungcheong for Group II. Those of cultivated soybeans were distinguished into Gyeonggi, Gangwon, and Gyeongsang for Group I, and Jeolla and Chungcheong for Group II. Therefore, the geographical relationships of wild soybeans were well typified based on the ecosystems of the Korean peninsula.

Evaluation of slope stability with Fabric Form (섬유거푸집을 적용한 비탈면의 안정성 평가)

  • Ahn, Kwang-Kuk;Choi, Young-Keun
    • Proceedings of the Korean Geotechical Society Conference
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    • 2005.10a
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    • pp.689-697
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    • 2005
  • The soil nailing system at permanent slope reinforcement is used with various facing methods in Korea. Also, pressure-injected grout technique is variously applied to many structures. However, most design of the pressure-injected grout technique have been carried out empirically because of complicated mechanisms associated with the behavior of surrounding soils and the hardening process of cement grout. Therefore this study, a newly modified soil nailing technology named as the PGSN (Pressure Grouting Soil Nailing) system with fabric form is developed to increase the global stability. Up to now, the PGSN system has been estimated mainly focusing on an establishment of the design procedure. In the present study, numerical study are carried out to evaluate potential failure surface and minimum factor of safety including facing stiffness and expanded radius of cemented grout by SSR (Shear Strength Reduction) technique. Also, results of numerical analysis are carried out for the typical section of soil nails slope using $FLAC^{2D}$ program for expanded effective radius by pressure grouting.

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Characteristics of Leaf Morphology and Genetic Variation of the Rare Woody Plant, Berchemia racemosa var. magna (희귀수종 먹넌출 엽의 형태적 특성과 유전변이)

  • Song, Jeong-Ho;Lim, Hyo-In;Jang, Kyung-Hwan;Han, Jingyu
    • Korean Journal of Plant Resources
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    • v.26 no.5
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    • pp.613-618
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    • 2013
  • This study was conducted to investigate the morphological characteristics of leaf and the genetic diversity of Berchemia racemosa var. magna which is only found in Anmyeon Island of South Korea. ANOVA test showed that there were significant differences among individuals within population in all 10 leaf characteristics. Average characteristics of 39 individuals were 11.8 cm in leaf length, 7.1 cm in leaf width, 1.67 in leaf index, 5.4 cm in upper 1/3 width, 6.2 cm in lower 1/3 width, 3.6 cm in petiole length, 0.19 mm in leaf thickness, 11.5 ea. in number of veins (left), 11.4 ea. in number of veins (right) and 61.7 $cm^2$ in leaf area, respectively. Except for leaf thickness (18.8%), petiole length (21.7%) and leaf area (22.0%), the coefficients of variation of most leaf characteristics were relatively low (<15.0%). A total of 50 bands was generated from 8 selected I-SSR primers. The estimates of genetic variation were 1.719 in effective number of alleles ($A_e$), 26.0% in proportion of polymorphic bands (P), 0.410 in expected heterozygosity ($H_e$) and 0.598 in Shannon's diversity index (S.I.), respectively. In spite of the small number and the limited distribution, the B. racemosa var. magna population in Anmyeon Island showed high genetic diversity.

Needle Characteristics and Genetic Variation of Pinus pumila Natural Population in Mt. Seorak (설악산 눈잣나무 천연집단의 침엽특성과 유전변이)

  • Song, Jeong-Ho;Lim, Hyo-In;Jang, Kyung-Hwan
    • Korean Journal of Plant Resources
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    • v.25 no.5
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    • pp.517-522
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    • 2012
  • This study was conducted to investigate the morphological and anatomical characteristics of needle and the genetic diversity of Pinus pumila Regel which is a unique and the southern peripheral population in South Korea. ANOVA test showed that there were significant differences among individuals within population in all 8 needle characteristics. Average characteristics of 66 individuals were 53.59 mm in needle length, 0.78 mm in needle width, 68.98 in needle index, 0.65 mm in needle thickness, 4.56 ea. in maximum stomata row, 3.80 ea. in minimum stomata row, 8.36 ea. in total stomata row and 1.71 ea. in resin canals, respectively. Resin canal per needle of this species ranged from one to three, depending on external type. Especially, arrangement types were 69.47% in two resin canals and 30.45% in a single resin canal. A total of 78 bands was generated from 9 selected I-SSR primers. The estimates of genetic variation were 61.5% in proportion of polymorphic bands (P), 1.698 in effective number of alleles ($A_e$), 0.388 in expected heterozygosity ($H_e$) and 0.567 in Shannon's information index (S.I.), respectively.

Suppression for Logistic Regression Model (로지스틱 회귀모형에서의 SUPPRESSION)

  • Hong C. S.;Kim H. I.;Ham J. H.
    • The Korean Journal of Applied Statistics
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    • v.18 no.3
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    • pp.701-712
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    • 2005
  • The suppression for logistic regression models has been debated no longer than that for linear regression models since, among many other reasons, sum of squares for regression (SSR) or coefficient of determination ($R^2$) could be defined into various ways. Based on four kinds of $R^2$'s: two kinds are most preferred, and the other two are proposed by Liao & McGee (2003), four kinds of SSR's are derived so that the suppression for logistic models is explained. Many data fitted to logistic models are generated by Monte Carlo method. We explore when suppression happens, and compare with that for linear regression models.