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http://dx.doi.org/10.7732/kjpr.2012.25.5.517

Needle Characteristics and Genetic Variation of Pinus pumila Natural Population in Mt. Seorak  

Song, Jeong-Ho (Department of Forest Genetic Resources, Korea Forest Research Institute)
Lim, Hyo-In (Department of Forest Genetic Resources, Korea Forest Research Institute)
Jang, Kyung-Hwan (Department of Forest Genetic Resources, Korea Forest Research Institute)
Publication Information
Korean Journal of Plant Resources / v.25, no.5, 2012 , pp. 517-522 More about this Journal
Abstract
This study was conducted to investigate the morphological and anatomical characteristics of needle and the genetic diversity of Pinus pumila Regel which is a unique and the southern peripheral population in South Korea. ANOVA test showed that there were significant differences among individuals within population in all 8 needle characteristics. Average characteristics of 66 individuals were 53.59 mm in needle length, 0.78 mm in needle width, 68.98 in needle index, 0.65 mm in needle thickness, 4.56 ea. in maximum stomata row, 3.80 ea. in minimum stomata row, 8.36 ea. in total stomata row and 1.71 ea. in resin canals, respectively. Resin canal per needle of this species ranged from one to three, depending on external type. Especially, arrangement types were 69.47% in two resin canals and 30.45% in a single resin canal. A total of 78 bands was generated from 9 selected I-SSR primers. The estimates of genetic variation were 61.5% in proportion of polymorphic bands (P), 1.698 in effective number of alleles ($A_e$), 0.388 in expected heterozygosity ($H_e$) and 0.567 in Shannon's information index (S.I.), respectively.
Keywords
Pinus pumila; Genetic variation; Needle; Resin canal; I-SSR markers;
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