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Analysis of Genetic Diversity and Population Structure for Core Set of Waxy and Normal Maize Inbred Lines using SSR Markers  

Sa, Kyu Jin (Department of Applied Plant Sciences, College of Agriculture and Life Science, Kangwon National University)
Kim, Jin-Ah (Department of Applied Plant Sciences, College of Agriculture and Life Science, Kangwon National University)
Park, Ki Jin (Maize Experiment Station, Kangwon Agricultural Research and Extension Services)
Park, Jong Yeol (Maize Experiment Station, Kangwon Agricultural Research and Extension Services)
Goh, Byeong Dae (Maize Experiment Station, Kangwon Agricultural Research and Extension Services)
Lee, Ju Kyong (Department of Applied Plant Sciences, College of Agriculture and Life Science, Kangwon National University)
Publication Information
Korean Journal of Breeding Science / v.43, no.5, 2011 , pp. 430-441 More about this Journal
Abstract
Maize is divided into two types based on the starch composition of the endosperm in the seed, normal maize(or non-waxy maize) and waxy maize. In this study, genetic diversity and population structure were investigated among 80 waxy maize and normal inbred lines(40 waxy maize inbred lines and 40 normal maize inbred lines) using 50 SSR markers. A total of 242 alleles were identified at all the loci with an average of 4.84 and a range between 2 and 9 alleles per locus. The gene diversity values varied from 0.420 to 0.854 with an average of 0.654. The PIC values varied from 0.332 to 0.838 with an average of 0.602. To evaluate the population structure, STRUCTURE 2.2 program was employed to confirm genetic structure. The 80 waxy and normal maize inbred lines were separated with based on the membership probability threshold 0.8, and divided into groups I, II and admixed group. The 13 waxy maize inbred lines were assigned to group I. The 45 maize inbred lines including 7 waxy maize inbred lines and 38 normal maize inbred lines were assigned to group II. The 22 maize inbred lines with 20 waxy maize inbred lines and 2 normal maize inbred lines were contained in the admixed group. The cluster tree generated using the described SSR markers recognized three major groups at 31.7% genetic similarity. Group I included 40 waxy maize inbred lines and 11 normal maize inbred lines, and Group II included 27 normal maize inbred lines. Group III consist of only 2 normal maize inbred lines. The present study has demonstrated the utility of SSR analysis for the study of genetic diversity and the population structure among waxy and normal maize inbred lines. The information obtained from the present studies would be very useful for designing efficient maize breeding programs in Maize Experiment Station, Kangwon Agricultural Research and Extension Services.
Keywords
SSR marker; Genetic diversity; Population structure; Waxy and normal maize inbred;
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