• Journal of the Korean Society of Marine Environment & Safety
• /
• v.27 no.6
• /
• pp.708-720
• /
• 2021

As hypoxia adversely affects the marine environment in northwestern Gamak Bay every summer, the present study determined its comprehensive occurrence mechanisms using the Multiple Regression Analysis (MRA) and suggested management directions based on the primary MRA factors. The first hypoxia occurred by thermocline related to weather conditions, with organic matter deposited inside the bay on 26^th June, 2017. Additionally, on 12^th July, halocline was also developed by increased rainfall, and the hypoxia was most expanded horizontally and vertically. The primary factors were the stratification and deposited organic matter. In contrast, the hypoxia correlated to phytoplankton growth and deposited organic matter on 8^th August was diminished with remarkably less precipitation. However, the stable halocline was caused by massive precipitation, and the reproduced phytoplankton re-generated the expanded hypoxia on 16^th August despite a short sampling interval. Subsequently, the hypoxia influenced by the deposited organic matter spread shallowly along the seafloor on 13^th September as the extinction period. These results suggest that stratification alleviation technologies, and the improvement and removal of the organic matter deposited on the surface sediment are necessary.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.49 no.6
• /
• pp.522-527
• /
• 2004

Barley growing in paddy fields often suffers from wet-injury due to oxygen deficiency in rhizospere caused by excessive water in the soil. This study was conducted to investigate responsiveness of growth, development and anaerobic glycolysis enzymes to acute hypoxia in barley seedlings. Barley seedlings at the third leaf stage were subjected to hypoxia (1 ppm dissolved oxygen) by sparging the culture solution with nitrogen gas for up to seven days. Length and fresh weights of the shoot and root were affected little by hypoxia for up to 5 days. But root dry weight was slightly decreased by hypoxia for 7 days. In the root, alcohol dehydrogenase and lactate dehydrogenase activities increased drastically under hypoxia, reaching at their maximum levels in 3 to 5 days of hypoxia and decreasing slightly thereafter. However, the activities of both enzymes changed little in the shoot. Increases of their activities in the root were contributed by all the isozymes found in barley. These results suggest that barley seedlings first adapt to hypoxia by rapidly activating fermentative glycolysis to stabilize cellular pH and to increase energy production for the following morphological adaptative changes.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.53 no.4
• /
• pp.440-446
• /
• 2008

Successful germination and establishment of seedlings in flooded paddy are critical in direct seeding cultivation of rice. In this study, we examined the relationship between antioxidant enzymes and alcohol dehydrogenase (ADH) activities and coleoptile elongation under submergence of deep water with two rice cultivars, Iksan429 and Woodrose, which show characteristic coleoptile elongation under hypoxic condition. The growth of shoot under submerged in water was faster than the root. The survival duration was longer in Iksan429 than in Woodrose under submerged in water. The alcohol dehydrogenase (ADH) activities were significantly increased under hypoxia compared to in aerated condition. The ADH activity was increased in Iksan429 more than in Woodrose under hypoxia. The superoxide dismutase (SOD) activity in Iksan429 was gradually increased up to 5 days after treatment (DAT) then decreased slowly till 14 DAT under water, whereas in Woodrose it was dramatically decreased after 5 DAT. The peroxidase (POX) activity in Iksan429 was significantly increased until 7 DAT under hypoxia, whereas it was not significantly different in Woodrose during hypoxic treatment. However, in non-treated condition, POX activity in Woodrose was increased more than Iksan429. The changes of catalase (CAT) activities showed no differences in both cultivars. We suggest that the overexpression of ADH, SOD and POX activities is responsible for the hypoxic tolerance and plays an important role in the surviving of rice seedling.

• Journal of Life Science
• /
• v.17 no.1 s.81
• /
• pp.143-149
• /
• 2007

Scutellaria baicalensis GEORGI(SB) is used in oriental medicine for the treatment of incipient strokes. Although it has been reported that SB is neuroprotective in a hypoxia model, its mechanism is poorly understood. Here, we investigated the effect of SB on the modulation of retinoic acid receptor a (RARa). Rat cerebrocortical cells were grown in neurobasal medium. On DIV12 cells were treated with SB $(20{\mu}g/ml)$ and given a hypoxic shock $(2%\;O_2/5%\;CO_2,\;3hr)$ on DIV14. In situ hybridization using cRNA probe revealed that RARa mRNA punctae are distributed, in addition to nucleus, throughout neuronal dendrites, where SB upregulated its density by 69.8% (p=0.001) and 129.8% (p=0.001) in both normoxia and hypoxia, respectively. At the protein level, SB upregulated RARa in the neuronal soma by 78.8% (p=0.004) and 23.6% (p=0.001) in both normoxia and hypoxia, respectively. These results indicate that SB upregulates RARa in both normoxia and hypoxia, which might contribute to the neuroprotection.

• Journal of Life Science
• /
• v.18 no.3
• /
• pp.311-317
• /
• 2008

Pathophysiological oxidative stress results in neuronal cell death mainly due to the generation reactive oxygen species (ROS). In low oxygen situation such as hypoxia and ischemia, excessive ROS is generated. Coptidis Rhizoma (CR) is a traditional medicine used for the incipient stroke. In this report we show that CR water extracts $(1\;{\mu}g/ml)$ exhibited protective effects of neuronal cell death in a hypoxic model (2% $O_2/5%\;CO_2,\;37^{\circ}C,$ 3 hr) of cultured rat cortical cells. We further show that CR water extracts significantly reduced the intensity of green fluorescence after staining with $H_2DCF-DA$ on one hour and three days after hypoxic shock and in normoxia as well. Our results indicate that CR water extracts prevent neuronal death by suppressing ROS generation.

• Journal of Korean Society of Coastal and Ocean Engineers
• /
• v.34 no.3
• /
• pp.47-57
• /
• 2022

We carried out studies on prediction in concentration of dissolved oxygen (DO) with LSTM model and prediction in occurrence of hypoxia water mass (HWM) with decision tree. As results of study on prediction in DO concentration, a large number of Hidden node caused high complexity of model and required enough Epoch. And it was high accuracy in long Sequence length as prediction time step increased. The results of prediction in occurrence of HWM showed that the accuracy of nonHWM case was 66.1% in 30 day prediction, it was higher than 37.5% of HWM case. The reason is that the decision tree might overestimate DO concentration.

• Journal of fish pathology
• /
• v.34 no.2
• /
• pp.213-224
• /
• 2021

Experiments were performed to investigate changes in metabolic rate (MO₂), critical oxygen saturation (S_crit) and hematological parameters of black rockfish, Sebastes schlegeli exposed to hypoxia at 15, 20 and 25℃. The MO₂ was measured at an interval of 10 min using intermittent-flow respirometry. The normoxic standard metabolic rate (SMR) was 116.5±5.5, 188.6±4.2 and 237.4±6.8 mg O₂/kg/hr, and S_crit was 22.1±1.2, 30.6±1.5 and 41.9±1.4% air saturation at 15, 20 and 25℃, respectively. Q₁₀ values were 2.62 between 15 and 20℃, 1.58 between 20 and 25℃, and 2.04 over the full temperature range. In the investigation of blood (hematocrit and hemoglobin) and biochemical parameters (plasma cortisol, glucose, electrolyte and osmolality), the rockfish were subjected to S_crit for each temperature during 4 hr. All of hematological parameters of the rockfish exposed to hypoxic water were significantly higher than those of normoxic control. Moreover, blood and biochemical parameters of the rockfish maintained to normoxic water showed the tendency of increase with temperature, and were significantly higher at 25C. As a result of this experiment, it was found that physiological stress due to hypoxia increased at high temperature.

• The Korean Journal of Malacology
• /
• v.24 no.1
• /
• pp.59-66
• /
• 2008

The ark shell, Scapharca broughtonii were collected from the Jinhae bay in Kyungnam, Korea from April 2006 to October 2007. To assess how much they have tolerance against hypoxia under the compound condition of temperature and salinity, survivorship, oxygen consumption rate and external features of Scapharca broughtonii were measured, The 7 days-$LC_{50}$ of dissolved oxygen (DO) for Scapharca broughtonii at $15^{\circ}C$-ordinary sea water and $26{\pm}1$ psu was 1.98mg/L (confidence limit: 1,45-2.39 mg/L) and 2.63mg/L (confidence limit: 1.76-3.37mg/L) respectively. Whereas 3 days-$LC_{50}$ at $25^{\circ}C$-ordinary sea water was 3.89mg/L (confidence limit: 3.36-4.55 mg/L) and 4 days-$LC_{50}$ at $25^{\circ}C-26{\pm}1$ psu was 3.74mg/L (confidence limit: 3.19-4,43 mg/L). Oxygen consumption rate with each experimental group was decreased during the period exposed to hypoxia. To recovery, they were replaced to ordinary and oxygen consumption rate was increased a little, but all died, The color of the umbo of shell in Scapharca broughtonii was changed dark during hypoxia. These data will provide important fundamental information for examining the causes of mass mortality of shellfish in the summer.

• Journal of Life Science
• /
• v.18 no.12
• /
• pp.1631-1636
• /
• 2008

Rhei Rhizoma (RR; 大黃) consists of the underground parts (rhizome and root) of Rheum officinale Baill. and Rheum palmatum L. (Polygonaceae), and is widely used in Southeast Asian folk medicine to alleviate liver and kidney damages. In this study, we investigated into the efficacy and mechanism of RR water extract in supporting neuronal survival in a hypoxia model of cultured rat hippocampal neurons. RR exhibited no cytotoxicity up to 10 ${\mu}g$/ml and exhibited neurosupportive effects at 2.5 ${\mu}g$/ml in normoxia. When RR was added to the culture media on 10 days in vitro (DIV10) and given a hypoxic shock (2% $O_2$/5% $CO_2$, 3 hr, $37^{\circ}C$) on DIV13, RR exhibited neuroprotective effects on 5 days post-shock. $H_2DCF$ stainings indicated that RR effectively prevents ROS production in both normoxia and hypoxia. JC-1 stainings showed that RR prevents dissipation of MMP in hypoxia. These results indicate that RR protects neurons by suppressing ROS production and MMP loss.

• Journal of the Korean Society of Marine Environment & Safety
• /
• v.20 no.5
• /
• pp.511-525
• /
• 2014

Goal of this study is estimating of validity of calculated vertical diffusion coefficient for Masan bay is semi-enclosed bay by using eco-hydrodynamic model that is used to analysis of physical structure of coastal waters and calculates the vertical diffusion coefficient. physical structure of coastal waters is calculated by EFDC model, vertical diffusion coefficient calculated as the density gradient is bigger, the vertical diffusion coefficient as density gradient is increases, the vertical diffusion coefficient is decreased. Validity of vertical diffusion coefficient estimated by reproducibility of concentration of dissolved oxygen that calculated in ecosystem model is constructed by Stella program. The Results of model in 2008~2009 were $R^2$ value of 2008 is 0.529~0.700 and $R^2$ value is 0.542~0.791. This results were similar to observed data and simulated to hypoxia at that time. The 'vertical diffusion coefficient' represents stratification and physical stable of a water body, and will be useful for prediction of Hypoxia outbreak.